LOCUS       RR209785                8175 bp    DNA     circular  SYN 
DEFINITION  Ppp1r9a (Myc-DDK-tagged ORF) - Rat protein phosphatase 1, regulatory (inhibitor) subunit 9A (Ppp1r9a), (10 ug)
ACCESSION   NM_053473
VERSION     NM_053473.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4330..4421 
                     /label="Myc-DDK"  
     misc_feature    4451..5036 
                     /label="PolyA signal"  
     misc_feature    4508..4527 
                     /label="Restriction Enzyme"  
     misc_feature    5312..5984 
                     /label="ColE1"  
     misc_feature    6414..7208 
                     /label="Kan/Neo"  
     misc_feature    7243..7545 
                     /label="SV40 ori"  
     misc_feature    7631..8108 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4305..4312 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4305 
                     /label="Ppp1r9a"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTGAAAGCT GAATCTTCAG GTGAACGAAC CACTCTCAGA AGTGCCTCTC 
     1081 CTCACAGGAA TGCATACAGA ACTGAGTTCC AGGCACTGAA AAGTACTTTT GACAAACCCA 
     1141 AACCTGATGG GGAACAGAAA ACGAAAGAAG GTGAGGGCTC CCAGCAGAGT AGGGGTAGGA 
     1201 AGTATGGCTC CAATGTCAAC AGAATTAAGA ACCTGTTCAT GCAGATGGGT ATGGAACCCA 
     1261 ACGAGAATGC TGCTATCATT GCCAAAACGA GAGGGAAAGG TAGGCCCTCA TCCCCTCAGA 
     1321 AGAGGATGAA GCCCAAAGAA TTTGTGGAGA AAACCGATGG CTCAGTGGTT AAACTGGAGT 
     1381 CCTCTGTCTC TGAACGGATT AGCAGATTTG ACACAATGCA CGATGGCCCT TCGTATGCTA 
     1441 AGTTCACAGA GACTCGAAAG ATGTTTGAGA GAAGTGGGCA TGAGTCAGGG CAAAATAATC 
     1501 GCCATTCCCC AAAGAAAGAG AAAGCGGGAG AGGCTGAGCC ACAGGATGAG TGGGGTGGCT 
     1561 CCAAGTCCAA TAGAGGCAGC AGTGATTCCT TGGACAGCCT GAGCCCTCGG ACGGAGGCTG 
     1621 TCTCTCCAAC TGTGAGCCAA CTGAGTGCCG TGTTTGAGAA CAGTGAGTCT CCAGGTGCCA 
     1681 TCACTCCTGG GAAGGCTGAG AACAGCAACT ACTCGGTGAC TGGGCATTAC CCCCTGAACT 
     1741 TACCATCTGT TACCGTAACC AATCTGGATA CCTTTGGCCG TCTCAAGGAT TCTAATTCAA 
     1801 GGCCTTCTTC AAACAAACAA GCTACCGACA CAGAAGAGCC TGAGAAGAGT GAGGCTGTGC 
     1861 CAGTACCGGA AGTGGCTCAG AAAGGTACCT CTTTAGCTTC ATTGCCTAGT GAAGAGAGAC 
     1921 AGCTGAGCAC GGAAGCTGAG GACGTCACAG CTCAACCAGA CACTCCGGAC AGCACTGATA 
     1981 AAGACAGTCC TGGAGAACCT TCTGCTGAAA GCCAGGCAAT GCCCAAGTCT AACACCCTCT 
     2041 CCCGACCGAA GGAGCCGTTA GAAGATGCTG AAGCTAATGT GGTCGGGAGT GAGGCAGAGC 
     2101 AGCCACAGAG GAGAGACCTG ACAGGTGGTG GTGACCTCAC CTCCCCGGAC GCGTCTGCTT 
     2161 CCAGCTGTGG GAAAGAAGTA CCTGAAGACT CGAACAGTTT TGAGGGCTCC CATGTATACA 
     2221 TGCATAGTGA CTATAACGTG TATCGGGTGA GATCCAGGTA TAACTCGGAC TGGGGAGAGA 
     2281 CGGGCACAGA GCAGGATGAG GGGGACGACA GCGATGAGAA CAATTACTAT CAGCCCGATA 
     2341 TGGAGTACTC GGAAATTGTT GGCTTGCCGC AAGAGGAAGA AATCCCAGCA AATAGGAAAA 
     2401 TTAAGTTTAG TTGTGCTCCG ATTAAGGTTT TCAACACGTA CTCCAATGAA GACTATGACA 
     2461 GGAGGAATGA TGACGTTGAC CCTGTGGCTG CATCTGCTGA GTACGAACTT GAAAAGCGAG 
     2521 TAGAAAAGCT GGAACTTTTC CCAGTGGAAC TGGAGAAAGA TGAGGATGGG CTTGGCATAA 
     2581 GCATTATTGG AATGGGCGTT GGTGCTGATG CTGGACTGGA GAAGCTGGGG ATATTCGTCA 
     2641 AGACAGTGAC TGAAGGAGGT GCTGCTCAGC GGGATGGCAG GATCCAAGTC AATGACCAGA 
     2701 TTGTGGAAGT GGATGGAATC AGCCTGGTGG GGGTCACACA GAATTTCGCT GCAACTGTTC 
     2761 TGCGGAACAC CAAGGGCAAC GTCAGGTTTG TTATTGGACG AGAAAAACCA GGACAAGTGA 
     2821 GTGAGGTTGC CCAGTTGATC AGCCAGACCC TGGAACAAGA GAGGAGACAG AGGGAGCTGC 
     2881 TCGAACGCCA CTACGCCCAG TATGATGCTG ACGACGATGA GACCGGAGAA TATGCCACTG 
     2941 ATGAAGAAGA AGATGAGGTT GGACCCATTC TTCCTGGTGG TGACATGGCC ATTGAAGTCT 
     3001 TCGAGCTCCC TGAGAATGAG GACATGTTTT CCCCATCTGA CTTGGATACA AGCAAGCTCA 
     3061 GTCACAAGTT CAAAGAGTTG CAAATCAAAC ATGCAGTTAC AGAAGCAGAG ATTCAAAAAT 
     3121 TGAAGACCAA GCTGCAAGCA GCAGAGAATG AGAAAGTAAG GTGGGAACTA GAAAAGAACC 
     3181 AACTGCAGCA GAATATTGAG GAGAATAAAG AAAGGATGGT AAAGCTGGAG AGCTACTGGA 
     3241 TCGAGGCGCA GACATTATGT CACACCGTGA ACGAGCATCT CAAAGAGACC CAAAGCCAGT 
     3301 ATCAGGCCTT GGAAAAGAAG TACAACAAAG CCAAGAAGTT GATCAAGGAT TTTCAACAAA 
     3361 AAGAGCTCGA TTTCATCAGG AGACAAGAAG TAGAAAGAAA GAAGCTGGAG GAGGTGGAAA 
     3421 AAGCTCACCT TGTTGAAGTC CAAGGCCTGC AAGTTCGGAT TAGAGATTTG GAGGCTGAGG 
     3481 TGTTCAGACT ACTAAAGCAA AATGGGACCC AAGTTAACAA CAACAACAAC ATCTTTGAGA 
     3541 GAAGACCATC TCCTGGGGAA GTCTCAAAAG GAGACACTAT GGAGAATGTG GAAGTCAAGC 
     3601 AAACATCCTG TCAGGACGGC TTGAGCCAAG ACTTGAATGA AGCAGTCCCA GAGACAGAGC 
     3661 GCCTGGATTC GAAAGCGCTG AAAACTCGGG CCCAGCTCTC TGTGAAGAAC AGGCGCCAGA 
     3721 GGCCCACAAG GACAAGGCTC TATGACAGCG TCAGCTCCAC TGATGGGGAG GACAGCCTGG 
     3781 AGCGGAAGAA TTTCACCTTC AATGATGACT TCAGTCCAAG CAGTACCAGT TCAGCAGACC 
     3841 TGAGCGGCTT AGGAGCAGAA CCCAAAACGC CAGGGCTCTC CCAGTCCTTG GCACTGTCCT 
     3901 CAGATGAGAG CCTGGACATG ATAGATGATG AGATCCTTGA CGATGGACAG TCTCCCAAAC 
     3961 ACACTCAGAG TCAGAGCCGG GCCGTCCATG AGTGGAGTGT GCAGCAGGTT TCACACTGGT 
     4021 TAGTGGGCCT CAGTCTGGAC CAGTATGTGT CTGAATTCAG TGCTCAGAAC ATCAGTGGTG 
     4081 AGCAGCTCCT GCAGCTGGAT GGCAATAAAC TGAAGGCTCT TGGAATGACA TCATCCCAGG 
     4141 ATCGGGCACT GGTTAAAAAA AAACTGAAGG AAATGAAGAT GTCTCTCGAG AAGGCTCGCA 
     4201 AGGCCCAGGA GAAAATGGAA AAACAAAGAG AAAAGCTGAG GAGGAAGGAA CAAGAGCAAA 
     4261 TGCAGAGGAA GTCTAAAAAG TCGGAAAAGA TGACTTCCAC AACCGAGCAA CCGAGCGGAC 
     4321 CGACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4381 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4441 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4501 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4561 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4621 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4681 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4741 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     4801 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     4861 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     4921 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     4981 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5041 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5101 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5161 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5221 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5281 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5341 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5401 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5461 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     5521 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     5581 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     5641 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     5701 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     5761 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     5821 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     5881 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     5941 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6001 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6061 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6121 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6181 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6241 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6301 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6361 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6421 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6481 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6541 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     6601 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     6661 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     6721 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     6781 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     6841 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     6901 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     6961 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7021 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7081 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7141 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7201 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7261 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7321 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7381 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7441 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7501 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7561 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     7621 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     7681 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     7741 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     7801 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     7861 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     7921 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     7981 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     8041 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8101 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8161 TTTAACGCGA ATTTT
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