LOCUS       RR209715                9270 bp    DNA     circular  SYN 
DEFINITION  Nos1 (Myc-DDK-tagged ORF) - Rat nitric oxide synthase 1, neuronal (Nos1), (10 ug)
ACCESSION   NM_052799
VERSION     NM_052799.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5427..5518 
                     /label="Myc-DDK"  
     misc_feature    5548..6133 
                     /label="PolyA signal"  
     misc_feature    5605..5624 
                     /label="Restriction Enzyme"  
     misc_feature    6409..7081 
                     /label="ColE1"  
     misc_feature    7511..8305 
                     /label="Kan/Neo"  
     misc_feature    8340..8642 
                     /label="SV40 ori"  
     misc_feature    8728..9205 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5409..5415 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5409 
                     /label="Nos1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAAGAGAAC ACGTTTGGGG TTCAGCAGAT CCAACCCAAT GTAATTTCTG 
     1081 TTCGTCTCTT CAAACGCAAA GTGGGAGGTC TGGGCTTCCT GGTGAAGGAA CGGGTCAGCA 
     1141 AGCCTCCCGT GATCATCTCA GACCTGATTC GAGGAGGTGC TGCGGAGCAG AGCGGCCTTA 
     1201 TCCAAGCTGG AGACATCATT CTCGCAGTCA ACGATCGGCC CTTGGTAGAC CTCAGCTATG 
     1261 ACAGTGCCCT GGAGGTTCTC AGGGGCATTG CCTCTGAGAC CCACGTGGTC CTCATTCTGA 
     1321 GGGGCCCTGA GGGCTTCACT ACACATCTGG AGACCACCTT CACAGGGGAT GGAACCCCCA 
     1381 AGACCATCCG GGTGACCCAG CCCCTCGGTC CTCCCACCAA AGCCGTCGAT CTGTCTCACC 
     1441 AGCCTTCAGC CAGCAAAGAC CAGTCATTAG CAGTAGACAG AGTCACAGGT CTGGGTAATG 
     1501 GCCCTCAGCA TGCCCAAGGC CATGGGCAGG GAGCTGGCTC AGTCTCCCAA GCTAATGGTG 
     1561 TGGCCATTGA CCCCACGATG AAAAGCACCA AGGCCAACCT CCAGGACATC GGGGAACATG 
     1621 ATGAACTGCT CAAAGAGATA GAACCTGTGC TGAGCATCCT CAACAGTGGG AGCAAAGCCA 
     1681 CCAACAGAGG GGGACCAGCC AAAGCAGAGA TGAAAGACAC AGGAATCCAG GTGGACAGAG 
     1741 ACCTCGATGG CAAATCGCAC AAAGCTCCGC CCCTGGGCGG GGACAATGAC CGCGTCTTCA 
     1801 ATGACCTGTG GGGGAAGGAC AACGTTCCTG TGGTCCTTAA CAACCCGTAT TCAGAGAAGG 
     1861 AACAGTCCCC TACCTCGGGG AAACAGTCTC CCACCAAGAA CGGCAGCCCT TCCAGGTGCC 
     1921 CCCGTTTCCT CAAGGTCAAG AACTGGGAGA CGGACGTGGT CCTCACCGAC ACCCTGCACC 
     1981 TGAAGAGCAC ACTGGAAACG GGGTGCACAG AGCACATTTG CATGGGCTCG ATCATGCTGC 
     2041 CTTCCCAGCA CACGCGGAAG CCAGAAGATG TCCGCACAAA GGACCAGCTC TTCCCTCTAG 
     2101 CCAAAGAATT TCTCGACCAA TACTACTCAT CCATTAAGAG ATTTGGCTCC AAGGCCCACA 
     2161 TGGACAGGCT GGAGGAGGTG AACAAGGAGA TTGAAAGCAC CAGCACCTAC CAGCTCAAGG 
     2221 ACACCGAGCT CATCTATGGC GCCAAGCATG CCTGGCGGAA CGCCTCTCGA TGTGTGGGCA 
     2281 GGATCCAGTG GTCCAAGCTG CAGGTGTTCG ATGCCCGAGA CTGCACCACA GCCCACGGCA 
     2341 TGTTCAACTA CATCTGTAAC CATGTCAAGT ATGCCACCAA CAAAGGGAAT CTCAGGTCGG 
     2401 CCATCACGAT ATTCCCTCAG AGGACTGACG GCAAACATGA CTTCCGAGTG TGGAACTCGC 
     2461 AGCTCATCCG CTACGCGGGC TACAAGCAGC CAGATGGCTC TACCTTGGGG GATCCAGCCA 
     2521 ATGTGCAGTT CACGGAGATC TGTATACAGC AGGGCTGGAA AGCCCCAAGA GGCCGCTTCG 
     2581 ACGTGCTGCC TCTCCTGCTT CAGGCCAATG GCAATGACCC TGAGCTCTTC CAGATCCCCC 
     2641 CAGAGCTGGT GCTGGAAGTG CCCATCAGGC ACCCCAAGTT CGACTGGTTT AAGGACCTGG 
     2701 GGCTCAAATG GTATGGCCTC CCCGCTGTGT CCAACATGCT GCTGGAGATC GGGGGCCTGG 
     2761 AGTTCAGCGC CTGTCCCTTC AGCGGCTGGT ACATGGGCAC AGAGATCGGC GTCCGTGACT 
     2821 ACTGTGACAA CTCTCGATAC AACATCCTGG AGGAAGTAGC CAAGAAGATG GATTTGGACA 
     2881 TGAGGAAGAC CTCGTCCCTC TGGAAGGACC AAGCACTGGT GGAGATCAAC ATTGCTGTTC 
     2941 TATATAGCTT CCAGAGTGAC AAGGTGACCA TCGTTGACCA CCACTCTGCC ACGGAGTCCT 
     3001 TCATCAAACA CATGGAGAAT GAATACCGCT GCAGAGGGGG CTGCCCCGCC GACTGGGTGT 
     3061 GGATTGTGCC TCCCATGTCG GGCAGCATCA CCCCTGTCTT CCACCAGGAG ATGCTCAACT 
     3121 ATAGACTCAC CCCGTCCTTT GAATACCAGC CTGATCCATG GAACACCCAC GTGTGGAAGG 
     3181 GCACCAACGG GACCCCCACG AAGCGGCGAG CTATCGGCTT TAAGAAATTG GCAGAGGCCG 
     3241 TCAAGTTCTC AGCCAAGCTA ATGGGGCAGG CCATGGCCAA GAGGGTCAAG GCGACCATTC 
     3301 TCTACGCCAC AGAGACAGGC AAATCACAAG CCTATGCCAA GACCCTGTGT GAGATCTTCA 
     3361 AGCACGCCTT CGATGCCAAG GCAATGTCCA TGGAGGAGTA TGACATCGTG CACCTGGAGC 
     3421 ACGAAGCCCT GGTCTTGGTG GTCACCAGCA CCTTTGGCAA TGGAGACCCC CCTGAGAACG 
     3481 GGGAGAAATT CGGCTGTGCT TTAATGGAGA TGAGGCACCC CAACTCTGTG CAGGAGGAGA 
     3541 GAAAGTACCC GGAACCCTTG CGTTTCTTTC CCCGTAAAGG GCCTTCCCTC TCCCATGTTG 
     3601 ACTCTGAAGC CCACAGTCTG GTTGCTGCCC GTGACAGCCA ACACAGGAGC TACAAGGTCC 
     3661 GATTCAACAG CGTCTCCTCC TATTCTGACT CCCGAAAGTC ATCGGGCGAC GGACCCGACC 
     3721 TCAGAGACAA CTTTGAAAGT ACTGGACCCC TGGCCAATGT GAGGTTCTCA GTGTTCGGCC 
     3781 TCGGCTCTCG GGCGTACCCC CACTTCTGTG CCTTTGGGCA TGCGGTGGAC ACCCTCCTGG 
     3841 AGGAACTGGG AGGGGAGAGG ATTCTGAAGA TGAGGGAGGG GGATGAGCTT TGCGGACAGG 
     3901 AAGAAGCTTT CAGGACCTGG GCCAAGAAAG TCTTCAAGGC AGCCTGTGAT GTGTTCTGCG 
     3961 TGGGGGATGA CGTCAACATC GAGAAGGCGA ACAACTCCCT CATTAGCAAT GACCGAAGCT 
     4021 GGAAGAGGAA CAAGTTCCGC CTCACGTATG TGGCGGAAGC TCCAGATCTG ACCCAAGGTC 
     4081 TTTCCAATGT TCACAAAAAA CGAGTCTCGG CTGCTCGACT CCTCAGCCGC CAAAACCTGC 
     4141 AAAGCCCTAA GTCCAGCCGA TCGACCATCT TCGTGCGTCT CCACACCAAC GGGAATCAGG 
     4201 AGCTGCAGTA CCAGCCAGGG GACCACCTGG GTGTCTTCCC CGGCAACCAC GAGGACCTCG 
     4261 TGAATGCACT CATTGAACGG CTGGAGGATG CACCGCCTGC CAACCACGTG GTGAAGGTGG 
     4321 AGATGCTGGA GGAGAGGAAC ACTGCTCTGG GTGTCATCAG TAATTGGAAG GATGAATCTC 
     4381 GCCTCCCACC CTGCACCATC TTCCAGGCCT TCAAGTACTA CCTGGACATC ACCACGCCGC 
     4441 CCACGCCCCT GCAGCTGCAG CAGTTCGCCT CTCTGGCCAC TAATGAGAAA GAGAAGCAGC 
     4501 GGTTGCTGGT CCTCAGCAAG GGGCTCCAGG AATATGAGGA GTGGAAGTGG GGCAAGAACC 
     4561 CCACAATGGT GGAGGTGCTG GAGGAGTTCC CGTCCATCCA GATGCCGGCT ACACTTCTCC 
     4621 TCACTCAGCT GTCGCTGCTG CAGCCTCGCT ACTACTCCAT CAGCTCCTCT CCAGACATGT 
     4681 ACCCCGACGA GGTGCACCTC ACTGTGGCCA TCGTCTCCTA CCACACCCGA GACGGAGAAG 
     4741 GACCAGTCCA CCACGGGGTG TGCTCCTCCT GGCTCAACAG AATACAGGCT GACGATGTAG 
     4801 TCCCCTGCTT CGTGAGAGGT GCCCCTAGCT TCCACCTGCC TCGAAACCCC CAGGTGCCTT 
     4861 GCATCCTGGT TGGCCCAGGC ACTGGCATCG CACCCTTCCG AAGCTTCTGG CAACAGCGAC 
     4921 AATTTGACAT CCAACACAAA GGAATGAATC CGTGCCCCAT GGTTCTGGTC TTCGGGTGTC 
     4981 GACAATCCAA GATAGATCAT ATCTACAGAG AGGAGACCCT GCAGGCCAAG AACAAGGGCG 
     5041 TCTTCAGAGA GCTGTACACT GTCTATTCCC GGGAACCGGA CAGGCCAAAG AAATATGTAC 
     5101 AGGACGTGCT GCAGGAACAG CTGGCTGAGT CTGTGTACCG CGCCCTGAAG GAGCAAGGAG 
     5161 GCCACATTTA TGTCTGTGGG GACGTTACCA TGGCCGCCGA TGTCCTCAAA GCCATCCAGC 
     5221 GCATAATGAC CCAGCAGGGG AAACTCTCAG AGGAGGACGC TGGTGTATTC ATCAGCAGGC 
     5281 TGAGGGATGA CAACCGGTAC CACGAGGACA TCTTTGGAGT CACCCTCAGA ACGTATGAAG 
     5341 TGACCAACCG CCTTAGATCT GAGTCCATCG CCTTCATCGA AGAGAGCAAA AAAGACGCAG 
     5401 ATGAGGTTTT CAGCTCCACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     5461 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     5521 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     5581 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     5641 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     5701 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     5761 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     5821 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     5881 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5941 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     6001 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     6061 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     6121 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     6181 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     6241 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     6301 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     6361 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     6421 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     6481 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     6541 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     6601 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     6661 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     6721 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     6781 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     6841 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     6901 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6961 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     7021 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     7081 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     7141 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     7201 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     7261 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     7321 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     7381 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     7441 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     7501 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     7561 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     7621 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     7681 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     7741 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     7801 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     7861 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     7921 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7981 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     8041 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     8101 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     8161 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     8221 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     8281 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     8341 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     8401 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     8461 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     8521 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8581 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8641 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     8701 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     8761 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     8821 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     8881 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     8941 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     9001 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     9061 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     9121 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     9181 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     9241 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//