LOCUS       RR209710                9216 bp    DNA     circular  SYN 
DEFINITION  Mlh3 (Myc-DDK-tagged ORF) - Rat mutL homolog 3 (E. coli) (Mlh3), (10 ug)
ACCESSION   NM_001108043
VERSION     NM_001108043.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5371..5462 
                     /label="Myc-DDK"  
     misc_feature    5492..6077 
                     /label="PolyA signal"  
     misc_feature    5549..5568 
                     /label="Restriction Enzyme"  
     misc_feature    6353..7025 
                     /label="ColE1"  
     misc_feature    7455..8249 
                     /label="Kan/Neo"  
     misc_feature    8284..8586 
                     /label="SV40 ori"  
     misc_feature    8672..9149 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5346..5353 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5346 
                     /label="Mlh3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATCAGGTGT CTGTCAGATG AAGTACAAGC CAAGTTGCGT TCTGGTTTAG 
     1081 CCCTAAGCTC CTTAGGCCAG TGTGTTGAGG AACTTACCCT TAACAGTATT GATGCTGAGG 
     1141 CAACATGTGT GGCCATCAGA GTGAATATGG AAACCTTCCA AGTTCAAGTG ATAGACAACG 
     1201 GACTTGGGAT GGCGGGGGAT GACGTGGAGA AGGTGGGGAA CCGGTATTTT ACTAGTAAAT 
     1261 GCCACTCAGT ACGGGACCTG GAGAACCCAA CGTTTTACGG TTTCCGAGGC GAGGCCTTGG 
     1321 CAAGTATAGC TGACATGGCT AGTGCTGTGG AAATTTCATC CAAGAAAAGC ACAACATTGA 
     1381 AGACCTTTGT GAAGATGTTT CAGAACGGAA AAGCCCTCGC CGCCCGTGAG GCGGACTTGA 
     1441 CCAGACCGAG TGTGGGGACT ACAGTGACGG TCTATAACCT GTTTTACCAG TTTCCTGTGC 
     1501 GGAGGAAAAG CATGGACCCT AGACTAGAGT TTGAGAAGGT TAGGCAGAGG GTGGAAGCCC 
     1561 TCTCACTTAT GCACCCCTCC ATTTCTTTCT CTTTGAGGAA TGACGTTTCT GGTTCCATGA 
     1621 TTCTTCAGCT CCCTAAAACC AAAGACATAT GTTCTCGGTT TTGTCAAATT TATGGATTGG 
     1681 GCAAGTCCCA AAAGTTAAGA GAAATACATT ATAAGTATAA GGAATTTGAG TTTAATGGCT 
     1741 ACATCAGCTC TGAAGCACAC TACAATAAGA ATATGCAGTT TTTGTTTGTG AACAGAAGAC 
     1801 TAGTTTTAAG AACAAAGTTG CATAAACTTA TTGACTTTTT ATTAAGGAAA GAAAGTATTA 
     1861 TATGCAGGCC AAAGAATGGC TCTGCCAGTA GGCAAATGAA TTCAAGTCCT CGACACCGTT 
     1921 CTGCCTCAGA ACTCCATGGG ATATATGTAA TCAACGTGCA GTGCCCGTTC TGTGAGTATG 
     1981 ATGTCTGCAT AGAGCCAGCC AAAACTCTGA TTGAATTCCA GAACTGGGAT ACCGTGTTGA 
     2041 TTTGTGTTCA GGAAGGAATA AAAAAGTTTT TAAAGCAAGA AAAATTATTT GTAGAATTAT 
     2101 CAAGTGAAGA TATTAAGGAA TTTAATGAAG ATAATGGTTT TAGTTTGATT GGTACAACAC 
     2161 TTCAGACACA GTCTACTCAT GAGAAGTGTG ACCAGAGCAG TTTCCAGGAA GCGTGTAATA 
     2221 ATATTTTGGA TTCCTATGAA ATGTTTAATT TGCAGTCAAA AGCTGTGAAA AGAATAGCTA 
     2281 CTTTAGAAAA TAGAACCACA CAAAATTCTG GCGATTCAGG AGCTATCAGA AAAAAGACAG 
     2341 AGGACTCATA CACTTATGAA TCAGATGACC TGGGCCATAG AAAAACTGTA GAGTCTGCGT 
     2401 TATACAACAG TGACAGTGCT TACTTAGGAT CAAGGATGTC AGAAGAAGAC ATAGCCAAAA 
     2461 CATCACACGC TGGAGAAAAT GAGAAATGGA AAAAATCTTT GTTGGAAAAT AGGACTTCAG 
     2521 AAAGGCCACA TGAAACCAGT CCAAAAATGT TTTCAAGCCC CATTCAAACT CATCATCCCC 
     2581 TTGAGGAGAG TGAGGCAGAT CTAGAAATAG AGATGAAAAG TAGTACTGTT AATGTCATGG 
     2641 CTGCCAACAT TCCTCCAAAT AGTGAAATTC AGAGTCAACT GGAGCAACTT GAAGATGCTC 
     2701 CTGAAGTGGA GTGCCAACCT CTGCCCTTTG AGACAACATT ATTAAGAGCA CAGGGTACTC 
     2761 TGAAAAAGAA GGAGAGAAAA AAGGAGCTCA GTAGCCATGG AAGAGTAAAT GTTTTTAGTT 
     2821 ATGGACAGGT TAAATTGTGC TCCACTGGCT TTATCACTCA TGTGGTACAA CGCGAACCCG 
     2881 CCAAGTCACC TGAAACAGAA CAGTTGTTTA AAAATTATGC TCGACCTGGT CCTGTAAGTG 
     2941 CCCAGGAAGC ATTTGGAAAT AGAACACACC ATGCAAGTGA GACTCCAGAC AGCAGTAGTT 
     3001 TAACAAGCAC TTTAAGTAAA GAATCCACTC AGCCGCCCCA CAAAAGATTC TGCAGAACAA 
     3061 ATACAGGTTA TGGGACAGAG AACAAACCTG TAGCAACAGA TGACAACTTG GCCCTTTTTC 
     3121 AGGGAAGCCG TAAAGAATCA CACACAGATT GCTTTTTGCC TGATACAACC TCCTTTCCGT 
     3181 GGTGTAGAAA TGTTTCCGGT GGTTATAAGA AAAAAGGTAA AGTGGGTTCC TTCAAACACG 
     3241 TAGTCCGTAG GAAGCTAAGC TTGCGTTCAC AAATAGGGTC TTTAGAGAAG TTTAAGAGGC 
     3301 AATATGGGAA GGTTAACAAT TCCCTAGCTG CAGAAGAGGA CAATGACACT GACTTCAGGA 
     3361 CCCATCTTGA GCCTCACAAT GAACCCGATG TTCTTCTGAA AGATAAGAGC CTCTCAGATA 
     3421 TGTCTGATGG TGGTGAGATC ACTCCATCAG AGCACAGTGA TGGGTGTCAA CCATTACGTC 
     3481 CCATCCTGTA CCCAGAGACA CTTTCCAAAG AAGATCGCTT AGAACAGAGG CCTAGTTTGA 
     3541 GAGAAAGTCC TATCACTCTG GCGGAATTGC TCCACGGTAA CAGAAAACCT TCTGAGGTTA 
     3601 AGAAGTCCAC TGAAGCCCTG GCTTCTAAAT TATCCAGACT CAAGGATTCT GAAAAAGAGA 
     3661 TGCAAACCAT GGAGATGGCA GGTCCTGTTA GTGAACGTCC AGACTCAGAT CCCAGTTGGA 
     3721 AAGACAATAG CCAGTGCGCT AGGCTGGACT TAAATTTTTG TGAATTATAT AAAAATAAAC 
     3781 TTGAAAAAAC AGAGAGTGAA GTGCTTCCAA TGCCAGATTC TGCCCCAGAG GATGATTCCA 
     3841 TCAATAAAAA CAGCGTGCTG TGTTCTAACA GTGTAACAGA TGACAGGGAG AAACCAGAAA 
     3901 CTTCCTTGCT GTTACCCTGT AGTGATTCTA AAATCAGCAA AGATTCAGAT GTTCTTATCA 
     3961 GAGTCTCAGA GCAACAGAGT GGAAGCCCTG ACTCTGCCAG CAGGGAGGTA ACGCGTCAGG 
     4021 TAGAAGATAG CGCCGGCAGC CAATGTGGAA TCTGTTCCCA GAGCCATGGG TTTAAAGCAA 
     4081 GATCTTGTTC TGACAATGAA GAATCAAGTA CGCACAGTAT GGACTGGCAG CAGCATTTTG 
     4141 ACGTAACCCT GGGAAGAACG GTTTACATCA ACAGAATGAC CGGGCTTAGC ACGTTTGTTG 
     4201 CTCCTACTGA TGACATTCAG ACTCCTTGTA CTAAAGATCT GACAACCGTG GCTGTGGATG 
     4261 TCCTACTTGG GAATGGGGTC CAGTACAGGT GTCATCCTTT TAGAAGCGAC CTTGTTCTTC 
     4321 CTTTCCTCCC AAGAGCTCAG GAAGAGAGGA CCAGGCGGAG ACACAACAGA GATGCTGTAG 
     4381 ATGCTGCTGC TGTCAGTGAA CCACTTCAGT CTTTGTTTTC AGAATGGAGC AATCCAGTGT 
     4441 TTGCCCGATA CCCAGAGGTT GCTGTTGACG TCAGCAGTGG CCAGGCTGGG AGCTTAGCCG 
     4501 TTAAGATTCA CAATGTTCTA TATCCCTATC GCTTCACCAA AGAGATGATC CACTCAGTGC 
     4561 AGGTTCTCCA GCAAGTGGAT AACAAGTTTA TCGCCTGCTT AATGAGCTCG AGGACGGACG 
     4621 GGAGTGGCCA AGCAGGTGGA AACCTGTTAG TCCTGGTGGA CCAGCACGCT GCCCATGAGC 
     4681 GCGTTCGTTT GGAGCAGCTA ATCACTGATT CCTATGAGAA ACAAGCTCCA CAAAGTGCTG 
     4741 GCCGGAAGAA ATTACTGTCT TCCACAATAA TTCCTCCACT AGCGATCACC GTGTCAGAGG 
     4801 AACAAAGGAG ACTCTTACGG TCTTACCACA AACATTTGGA AGATCTGGGA CTTGAGTTGC 
     4861 TCTTTCCAGA TGCTAGTGAC TCTTCGATCC TGGTGGGAAA AGTGCCACTC TGCTTTGTAG 
     4921 AAAGAGAAGC TAATGAACTT CGAAGAGGAC GATCTCCTGT GACTAAGAGT ATTGTGGAGG 
     4981 AATTGATTCG AGAACAGCTG GAGCTGCTCC AGACCACGGG AGGTATCCAA GGGACACTGC 
     5041 CGCTGACTGT CCAGAAGGTG TTGGCCTCCC AGGCCTGCCA CGGGGCCATT AAGTTTAATG 
     5101 ATCGTCTGAG TCTGGAGGAG AGCTGTCGGC TTATCGAAGC TCTGTCCTTG TGCCAGCTGC 
     5161 CATTTCAGTG TGCTCATGGG CGACCTTCAA TGCTGCCCTT AGCTGACCTG GACCACTTGG 
     5221 AGCAGGAAAA ACAGGTTAAA CCCAATCTTA CTAAACTTCG CAAAATGGCT CATGCCTGGC 
     5281 ATCTCTTTGG AAAAGCAGAA GGCTGTGATA CAAAGCAGGA GCCACAACAG CCCGTACCTC 
     5341 CTTGTGAGCC ACCGAGCGGA CCGACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     5401 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     5461 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     5521 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     5581 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     5641 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     5701 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     5761 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     5821 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5881 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5941 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     6001 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     6061 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     6121 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     6181 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     6241 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     6301 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     6361 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     6421 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     6481 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     6541 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     6601 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     6661 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     6721 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     6781 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     6841 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6901 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6961 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     7021 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     7081 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     7141 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     7201 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     7261 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     7321 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     7381 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     7441 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     7501 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     7561 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     7621 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     7681 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     7741 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     7801 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     7861 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7921 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7981 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     8041 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     8101 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     8161 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     8221 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     8281 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     8341 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     8401 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     8461 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8521 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8581 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     8641 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     8701 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     8761 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     8821 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     8881 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     8941 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     9001 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     9061 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     9121 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     9181 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//