LOCUS       RR209701                8874 bp    DNA     circular  SYN 
DEFINITION  Wdr33 (Myc-DDK-tagged ORF) - Rat WD repeat domain 33 (Wdr33), (10 ug)
ACCESSION   NM_001107398
VERSION     NM_001107398.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5031..5122 
                     /label="Myc-DDK"  
     misc_feature    5152..5737 
                     /label="PolyA signal"  
     misc_feature    5209..5228 
                     /label="Restriction Enzyme"  
     misc_feature    6013..6685 
                     /label="ColE1"  
     misc_feature    7115..7909 
                     /label="Kan/Neo"  
     misc_feature    7944..8246 
                     /label="SV40 ori"  
     misc_feature    8332..8809 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5013..5019 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5013 
                     /label="Wdr33"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTACAGAA ATTGGCTCTC CTCCACGATT TTTCCATATG CCGAGGTTCC 
     1081 AGCACCAGGC TCCCCGACAG TTGTTCTATA AGCGGCCTGA CTTTGCACAG CAGCAGGCAA 
     1141 TGCAGCAGCT CACCTTTGAC GGGAAGCGGA TGAGAAAGGC CGTGAACCGG AAAACCATAG 
     1201 ACTACAACCC CTCTGTCATT AAGTATTTGG AGAATAGGAT ATGGCAAAGA GATCAGAGAG 
     1261 ATATGCGGGC AATTCAGCCG GATGCAGGTT ATTATAATGA TCTGGTCCCA CCTATAGGAA 
     1321 TGTTGAATAA TCCTATGAAT GCAGTAACAA CGAAATTTGT TAGGACATCA ACAAACAAAG 
     1381 TAAAATGCCC AGTGTTTGTT GTGAGGTGGA CCCCGGAAGG AAGACGGTTG GTCACTGGAG 
     1441 CTTCTAGTGG GGAGTTCACC TTGTGGAACG GGCTGACTTT CAACTTTGAA ACCATATTGC 
     1501 AGGCTCATGA TAGCCCTGTG AGGGCCATGA CTTGGTCACA TAATGACATG TGGATGTTGA 
     1561 CAGCAGATCA TGGAGGATAT GTGAAATATT GGCAATCCAA CATGAACAAC GTCAAGATGT 
     1621 TCCAGGCACA TAAGGAGGCG ATTAGAGAGG CCAGTTTCTC ACCCACGGAT AATAAATTTG 
     1681 CTACATGCTC TGATGACGGC ACTGTTAGAA TCTGGGATTT TCTTCGTTGC CATGAGGAAA 
     1741 GAATTCTCCG AGGTCATGGT GCTGATGTGA AGTGTGTAGA TTGGCATCCA ACCAAAGGGT 
     1801 TGGTTGTTTC AGGAAGTAAA GATAGCCAGC AGCCAATCAA GTTCTGGGAC CCCAAGACTG 
     1861 GGCAGAGTCT TGCAACACTT CACGCCCATA AAAACACGGT GATGGAAGTG AAATTAAACC 
     1921 TCAACGGCAA CTGGCTCCTC ACGGCCTCTC GTGACCATCT CTGTAAGCTT TTCGATATCC 
     1981 GAAACCTGAA AGAAGAGCTT CAGGTATTCC GAGGTCACAA GAAGGAAGCC ACAGCTGTAG 
     2041 CCTGGCATCC TGTCCACGAA GGACTTTTTG CCAGCGGAGG ATCCGACGGC TCCTTGTTAT 
     2101 TCTGGCACGT TGGGGTGGAG AAGGAAGTAG GTGGGATGGA GATGGCCCAC GAAGGGATGA 
     2161 TCTGGAGTTT GGCTTGGCAT CCTCTGGGAC ATATTCTCTG CTCGGGCTCA AATGACCATA 
     2221 CCAGCAAATT CTGGACTCGA AATCGGCCAG GTGACAAAAT GCGAGATCGA TATAATCTGA 
     2281 ACCTTTTACC TGGCATGTCA GAAGACGGAG TAGAGTATGA TGACCTGGAA CCTAATAGCC 
     2341 TGGCAGTGAT TCCAGGGATG GGAATCCCAG AACAACTGAA ACTGGCCATG GAGCAGGAGC 
     2401 AGATGGGGAA AGATGAATCA AATGACATTG AAATGACAAT TCCAGGCTTG GATTGGGGAA 
     2461 TGGAAGAAGT AATGCAAAAG GATCAGAAAA AAGTTCCTCA GAAGAAAGTC CCTTATGCAA 
     2521 AGCCCATTCC TGCTCAGTTC CAGCAGGCCT GGATGCAAAA TAAAGTTCCA ATTCCTGCTC 
     2581 CAAATGAGGT GCTGAATGAC AGGAAAGAGG ATATTAAGCT AGAAGAGAAG AAAAAAACAC 
     2641 AAGCAGAAAT CGAGCAAGAA ATGGCCACGC TGCAGTATAC CAACCCACAG CTTTTAGAGC 
     2701 AACTTAAAAT TGAAAGGCTT GCCCAGAAGC AGGCCGATCA GATCCAGCCT CCTCCCTCGT 
     2761 CTGGTACTCC TCTCCTCGGA CCTCAGCCCT TTTCTGGTCA AGGCCCAATG TCTCAGATCC 
     2821 CTCAAGGTTT TCAGCAGCCT CACCCATCTC AGCAGATGCC ATTGGTGGCT CAAATGGGGC 
     2881 CTCCAGGTCC ACAGGGCCAG TTCAGAGCCC CTGGACCCCA GGGACAAATG GGACCACAAG 
     2941 GCCCTCCATT GCATCAAGGA GGTGGGGGGC CACAAGGATT CATGGGACCA CAAGGACCCC 
     3001 AGGGCCCTCC CCAGGGTCTG CCACGGCCTC AGGACATGCA TGGTCCACAA GGAATGCAGA 
     3061 GGCACCCTGG ACCTCATGGC CCTTTGGGAC CTCAAGGGCC ACCCGGACCT CAAGGTAGTT 
     3121 CTGGTCCTCA AGGTCATATG GGTCCTCAGG GTCCACCAGG ACCTCAGGGT CACATTGGCC 
     3181 CCCAAGGCCC ACCTGCTCCT CAGGGCCATA TGGGCCCACA GGGTCCTCCT GGTACTCAAG 
     3241 GAATGCAGGG ACCACCTGGT CCCAGAGGAA TGCAAGGACC TCCTCATCCT CATGGGATAC 
     3301 AGGGTGGGCC AACATCTCAA GGGATCCAGG GACCTCTGAT GGGACTGAAT CCAAGAGGAA 
     3361 TGCAGGGACC CCCAGGACCT CGGGAGAATC AGGGTCCTGC TCCTCAAGGG ATAATGATTG 
     3421 GCCATCCACC TCAGGAGATG AGAGGACCAC ATCCTCCAAG TGGACTGCTG GGACATGGCC 
     3481 CTCAGGAAAT GAGAGGTCCT CAGGAGATGC GGGGCATGCA GGGGCCTCCA CCACAGGGGT 
     3541 CAATGTTGGG CCCTCCCCAG GAACTTCGAG GACCCTCAGG TTCACAGGGG CAGCAGGGGC 
     3601 CACCCCAGGG CTCTTTGGGA CCTCCACCCC AGGGTGGCAT GCAAGGACCC CCTGGACCTC 
     3661 AGGGACAACA GAACCCGGCA AGAGGGCCAC ATCCATCTCA AGGGCCAATA CCATTCCAGC 
     3721 AGCAGAAAGC ACCTCTGCTA GGTGATGGGC CCCGGGCCCC CTTCAATCAG GAAGGACAGA 
     3781 GTACAGGTCC CCCACCCTTG ATACCAGGCC TAGGGCAACA GGGAGCACAA GGCCGCATTC 
     3841 CTCCTCTGAA CCCTGGACAA GGACCCGGCC CTAACAAAGG TGACTCTCGA GGCCCTCCCA 
     3901 ACCATCACCT GGGCCCCATG TCAGAGAGGC GGCATGAGCA GAGCGGTGGT CCTGAGCATG 
     3961 GTCCTGACAG GGGACCTTTC CGGGGTGGCC AGGACTGCAG GGGTCCTCCT GACAGGCGTG 
     4021 GCTCTCACCC TGATTTCCCT GATGACTTCA GCAGACCAGA TGACTTCCAT CCTGACAAGC 
     4081 GCTTTGGCCA CCGGTTACGG GAATTCGAGG GACGAGGAGG ACCCTTACCA CAAGAAGAAA 
     4141 AGTGGAGGCG AGGGGGCCCT GGACCTCCCT TTCCACCTGA TCACAGGGAG TTCAATGAGG 
     4201 GGGACGGCCG GGGTGCAGCC CGAGGCCCTC CAGGGGCATG GGAAGGCCGC AGACCTGGAG 
     4261 ACGAACGATT CCCCCGGGAT CCTGATGACC CACGATTTCG AGGGCGCAGA GAAGAAAGCT 
     4321 TTAGAAGAGG TGCTCCCCCA AGACACGAAG GCCGTGCTCC TCCAAGAGGA AGGGACAACT 
     4381 TTCCTGGTCC TGATGACTTT GGTCCAGAAG AGGTTTTCGA TGCTTCTGAT GAAGCAGCCC 
     4441 GAGGACGAGA TCTCAGAGGT CGAGGCCGTG GCACCCCTAG AGGGGGATCC AGGAAGTGCT 
     4501 TACTCCCCAC TCCTGATGAG TTTCCTCGCT TTGAAGGAGG ACGGAAGCCA GACTCCTGGG 
     4561 ATGGAAACCG AGAGCCCGGA CCAGGTCATG AACACTTTCG TGATGCTCCC CGTCCTGATC 
     4621 ATCCCCCTCA CGATGGCCAT TCACCAGCTA GCAGAGAGCG CTCCTCTTCT CTCCAAGGCA 
     4681 TGGACATGGC CTCCCTACCA CCCCGGAAGC GCCCCTGGCA TGATGGGTCG GGCACTTCTG 
     4741 AACACAGAGA GATGGAGGCC CAAGGAGGCC CCTCGGAGGA CCGAGGAAGC AAAGGCCGAG 
     4801 GGGGCCCAGG ACCTTCCCAG AGAGTTCCAA AATCCGGGCG TTCCAGCTCT TTGGACGGAG 
     4861 ACCATCATGA TGGATACCAC AGAGATGAAC CTTTTGGTGG CCCTCCAGGC AGCAGCTCAT 
     4921 CTTCTCGAGG GGGCCGGAGT GGCAGTAACT GGGGTAGAGG GAGTAACATG AACTCTGGCC 
     4981 CTCCAAGGAG AGGCACTTCA AGAGGTAGTG GGAGGGGTCG GACGCGTACG CGGCCGCTCG 
     5041 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     5101 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     5161 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     5221 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     5281 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     5341 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     5401 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5461 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5521 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5581 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5641 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5701 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5761 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5821 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5881 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5941 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     6001 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     6061 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     6121 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     6181 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     6241 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     6301 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     6361 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6421 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6481 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6541 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6601 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6661 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6721 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6781 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6841 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6901 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6961 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     7021 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     7081 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     7141 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     7201 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     7261 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     7321 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     7381 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7441 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7501 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7561 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7621 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7681 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7741 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7801 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7861 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7921 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7981 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     8041 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     8101 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8161 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     8221 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     8281 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     8341 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     8401 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8461 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8521 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8581 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8641 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8701 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8761 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8821 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//