LOCUS       RR209678                8187 bp    DNA     circular  SYN 
DEFINITION  Kcnh8 (Myc-DDK-tagged ORF) - Rat potassium voltage-gated channel, subfamily H (eag-related), member 8 (Kcnh8), (10 ug)
ACCESSION   NM_145095
VERSION     NM_145095.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4344..4435 
                     /label="Myc-DDK"  
     misc_feature    4465..5050 
                     /label="PolyA signal"  
     misc_feature    4522..4541 
                     /label="Restriction Enzyme"  
     misc_feature    5326..5998 
                     /label="ColE1"  
     misc_feature    6428..7222 
                     /label="Kan/Neo"  
     misc_feature    7257..7559 
                     /label="SV40 ori"  
     misc_feature    7645..8122 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4326..4332 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4326 
                     /label="Kcnh8"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCGGTTATG AAAGGATTGC TGGCGCCACA GAACACCTTC CTGGACACTA 
     1081 TTGCCACCCG TTTCGACGGA ACACATAGCA ACTTCATCCT GGCCAATGCC CAAGTGGCAA 
     1141 AAGGTTTCCC CATAGTCTAC TGTTCAGATG GCTTCTGTGA GCTGGCTGGG TTTGCTCGAA 
     1201 CTGAAGTCAT GCAGAAGAGT TGCAGCTGCA AGTTTCTGTT TGGGGTGGAG ACCAACGAGC 
     1261 AGCTGATGCT TCAGATCGAA AAGTCCCTGG AGGAGAAGGT AGAGTTCAAA GGAGAAATTA 
     1321 TGTTCTACAA GAAGAATGGG GCTCCATTTT GGTGCCTGTT GGATATCGTT CCTATAAAGA 
     1381 ATGAGAAAGG AGATGTGGTC CTTTTCCTGG CCTCATTCAA AGATATAACA GACACGAAAG 
     1441 TGAAGATTAC TTCAGAAGAT AAAAAAGAAG ACAGAGCCAA AGGAAGATCA AGAGCAGGGA 
     1501 GCCACTTCGA CTCAGCCCGC AGACGGAGCC GAGCAGTCCT TTATCACATC TCAGGACACC 
     1561 TGCAAAGAAG AGAAAAGAAC AAATTGAAAA TAAATAATAA CGTGTTTGTA GATAAACCGG 
     1621 CGTTTCCAGA GTATAAGGTT TCCGATGCAA AAAAGTCCAA GTTCATCCTG CTGCACTTCA 
     1681 GCACTTTTAA AGCTGGCTGG GACTGGCTCA TTTTGCTGGC AACGTTTTAT GTTGCTGTGA 
     1741 CAGTCCCTTA CAACGTGTGC TTCATTGGCA ATGAGGATCT GTCCACAACT CGGAGCACAA 
     1801 CGGTCAGTGA CATCGCTGTG GAGATTCTCT TCATTATAGA TATTATTCTA AATTTCCGAA 
     1861 CAACTTATGT CAGCAAGTCT GGCCAAGTTA TCTTTGAAGC GAGATCCATT TGCATCCACT 
     1921 ACGTCACCAC CTGGTTCATC ATTGATCTGA TTGCTGCCCT GCCCTTTGAC CTCCTGTATG 
     1981 CTTTCAATGT CACAGTGGTG TCCCTCGTAC ATCTTCTGAA GACTGTTCGG CTGCTCCGTC 
     2041 TTTTGCGCCT CCTGCAGAAG CTGGACCGTT ATTCTCAGCA CAGCACAATT GTCCTCACCC 
     2101 TGCTCATGTC CATGTTTGCT CTCCTTGCAC ACTGGATGGC ATGTATCTGG TATGTCATTG 
     2161 GAAAAATGGA GAGGGAGGAC AACAGCCTTC TCAAGTGGGA AGTCGGTTGG CTTCACGAGC 
     2221 TGGGAAAGAG ACTGGAATCT CCGTACTACG GCAACAACAC GCTGGGCGGC CCGTCCATCC 
     2281 GCAGTGCCTA TATCGCAGCC TTGTACTTCA CTCTCAGCAG CCTCACCAGC GTGGGATTTG 
     2341 GGAATGTGTC CGCTAACACG GATGCAGAGA AGATCTTCTC CATCTGTACC ATGTTGATTG 
     2401 GAGCCCTGAT GCATGCCTTG GTATTTGGGA ATGTGACTGC CATCATACAG AGAATGTACT 
     2461 CTAGATGGAG CCTGTACCAT ACTAGAACCA AGGACCTAAA GGACTTCATC CGTGTGCATC 
     2521 ACCTGCCCCA GCAACTCAAG CAGAGGATGC TTGAGTACTT TCAGACAACT TGGTCTGTCA 
     2581 ACAATGGAAT AGATTCAAAT GAGCTTTTGA AAGACTTTCC AGATGAGCTG CGCTCTGACA 
     2641 TCACAATGCA TCTGAACAAG GAGATCTTAC AGCTGTCCCT GTTTGAATGT GCTAGCCGGG 
     2701 GCTGCCTCAG GTCTCTGTCT CTCCATATTA AAACCTCATT CTGTGCCCCA GGAGAGTATC 
     2761 TGCTGCGCCA GGGAGATGCG TTGCAGGCCA TCTACTTCGT GTGCTCAGGC TCTATGGAGG 
     2821 TTCTTAAAGA CAGCATGGTG TTGGCTATTC TAGGGAAGGG GGATTTAATT GGAGCAAATT 
     2881 TATCAATTAA AGACCAAGTG ATCAAGACCA ACGCTGACGT GAAGGCTCTG ACCTACTGTG 
     2941 ATCTACAGTG CATCATCCTG AAAGGTCTCT TTGAGGTGCT GGGCCTTTAC CCAGAGTACG 
     3001 CACACAAATT CGTAGAAGAC ATCCAGCACG ACCTCACATA CAACCTTCGA GAAGGTCATG 
     3061 AGAGTGATGT AATATCAAGA TTATCGAACA AATCTACAGT CCCACAGGCA GAGCCCAAGG 
     3121 GGAATGGAAG CATCAAGAAG AGACTCCCAT CCATTGTGGA AGATGAGGAA GAGGAGGAAG 
     3181 TGGAGGAAGA GGAGACCACC TCCCTTTCTC CCATCTACAC AAGGGGATCC TCTGTTTCAC 
     3241 ACAGCAAAAA GACTGGAAGC AGTAAGAGCT ATCTAGGTTT GAGCTTAAAG CAACTGACCT 
     3301 CAGGAACAGT TCCATTCCAC TCACCTATCA GAGTCTCCAG TGCCAACTCC CCTAAAACCA 
     3361 AGCAGGAAGC TGACCCACCT AACCATGGCA CACGGAAAGA GAAGAATCTG AAAGTTCAGC 
     3421 TCTGCAGCCT GGGTACTGCT GGAACCCCAG AGCTCAGTCC GAGGATTGTC GATGGAATTG 
     3481 AAGATGGCAA CAGCAGTGAG GAAACTCAGA CTTTTGATTT TGGCTCTGAA CAAATCAGGC 
     3541 CAGAGCCCAG GATTTCCCCT TCCCTTGGAG AATCAGAGAT TGGAGCTGCG TTTCTGTTCA 
     3601 TCAAGGCTGA AGAAACCAAG CAGCAGATAA ACAAGCTCAA CAGTGAGGTC ACAACATTGA 
     3661 CTCAGGAGGT CTCCCAGCTA GGGAAAGATA TGAGAAGCAT CATGCAACTT CTGGAAAACA 
     3721 TCTTGTCACC TCAGCAGCCA TCACAATTTT GTTCTCTGCA TCCCACTTCA ATCTGTCCTT 
     3781 CCAGAGAAAG TTTCCAGACT AGGGTGAGCT GGAGTGCTCA CCAGCCTTGC CTACACTTGC 
     3841 AGGCAAATGG AGCACATCTT TACCATGGCA ATGTCACCTC TGACATCTGG AGTGTCGACC 
     3901 CCTCCTTGGT GGGCAGCAAC CCTCAACGAA CTGAAGCTCA TGAGCAAAGT CCAGTAGATA 
     3961 GTGAACTGCA TCATTCTCCA AACCTGGCTT ATTCCCCCTC TCACTGCCAG GTTATCCAAG 
     4021 AAGGCCACTT GCAGTTCCTA AGGTGCATCT CCCCTCATTC AGATACCACA CTGACACCTT 
     4081 TGCAGTCCAT CTCAGCCACT CTCTCATCCT CTGTGTGCTC CTCATCAGAA ACATCCTTGC 
     4141 ACCTCGTTCT CCCAAGTAGG TCAGAGGAGG GCAGCATCAC TCATGGACCT GTGAGTTCTT 
     4201 TCAGTTTGGA AAACTTACCA GGATCTTGGG ACCGAGAAGG AATGATGTCA GCCTCTACAG 
     4261 AACCCTTGGA GAACTTTCCA GTAGAAGTTG TCACAAGCAC AGCGGATGTA AAAGACAGCA 
     4321 AAGCCATAAA CGTAACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4381 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     4441 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4501 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4561 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4621 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4681 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4741 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     4801 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     4861 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     4921 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     4981 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5041 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5101 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     5161 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5221 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5281 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     5341 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     5401 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     5461 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     5521 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     5581 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     5641 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     5701 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     5761 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     5821 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     5881 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     5941 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6001 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6061 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6121 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6181 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6241 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6301 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6361 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     6421 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6481 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     6541 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     6601 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     6661 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     6721 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     6781 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     6841 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     6901 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     6961 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7021 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7081 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7141 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7201 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7261 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7321 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7381 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     7441 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7501 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7561 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     7621 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     7681 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     7741 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     7801 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     7861 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     7921 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     7981 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     8041 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     8101 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     8161 ATTTAACAAA AATTTAACGC GAATTTT
//