LOCUS       RR209659                7173 bp    DNA     circular  SYN 
DEFINITION  Iws1 (Myc-DDK-tagged ORF) - Rat IWS1 homolog (S. cerevisiae) (Iws1), (10 ug)
ACCESSION   NM_001034918
VERSION     NM_001034918.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3330..3421 
                     /label="Myc-DDK"  
     misc_feature    3451..4036 
                     /label="PolyA signal"  
     misc_feature    3508..3527 
                     /label="Restriction Enzyme"  
     misc_feature    4312..4984 
                     /label="ColE1"  
     misc_feature    5414..6208 
                     /label="Kan/Neo"  
     misc_feature    6243..6545 
                     /label="SV40 ori"  
     misc_feature    6631..7108 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3312..3318 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3312 
                     /label="Iws1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACTCGGAG TACTACAGCG GTGACCAGTC AGATGATGGT GGTGCAACCC 
     1081 CAGTACAGGA CGAGCGGGAT TCAGGGTCAG ATGGTGAGGA TGATGTAAAC GAGCAGCACT 
     1141 CCGGATCCGA CACTGGAAGT GTAGACCGCC ATTCAGAGAA TGAAACTAGT GATAGAGAAG 
     1201 ATGGCTTGAC CAAGATACAT AATGGAACAG ACTCTGAGAA TGATGAGCCC TCCAATGTTC 
     1261 ATGCCAGTGA CTCTGAAAGT GAGGAGCTTC ACAGGCCAAA AGACAGTGAC TCTGAATCCG 
     1321 AGGAACATGC AGAGTCTCCT GCCAGTGATT CTGAAAACGA AGCTGTCCAC CAGCAGGGGA 
     1381 GTGACTCTGA AAAGGAAGAG CTTCTTAATG GGCATGCAAG TGACTCTGAA AAGGAAGAGG 
     1441 GTAGAAAGCA TGCAGCCAGT GACTCTGAAA CCGAGGACAC TCTGCAGCCT CAGGGCAGCG 
     1501 AGTCTGACAG TGAAGATCCC CCAAGGCCAC AGGCCAGTGA TTCAGAAAGC GAGGAGCCTC 
     1561 CCAAACCTCG GATCAGTGAT TCAGAAAGCG AGGAGCTCCC CAAACCTCGG ATCAGTGACT 
     1621 CAGAAAGTGA GGATCCTCCA AGGCCCCAGG TCAGTGACTC AGAAAGTGAG GAGCTCCCCA 
     1681 AACCCCGAGT CAGTGATTCA GAAAGTGAGG ATCCCCCAAG GCCACAGGCT AGTGACTCGG 
     1741 AAAGTGAGGA GCTCCCCAAA CCCCGAGTCA GCGACTCGGA AAGTGAGGAC CCTCAGAAAG 
     1801 GACCTGCTAG TGATTCTGAA GCTGAGGATG CCTCCAGACA TAAAGAAAAG CCAGAGTCAG 
     1861 AGGACAGTGA TGGGGAGAAT AAGAGGGAAG ACTCTGAAGT GCAGAATGAG TCAGACGGCC 
     1921 ATGCAGACAG AAAAGGACTC CACAGCTCTG ACAGCGAGGA GGAAGAACCC AAGAGGCAAA 
     1981 AGATTGACAG TGATGACGAC GGAGAAAAGG AGGGGGATGA GAAAGTAGCA AAGCGGAAGG 
     2041 CTGCCGTGCT TTCTGATAGT GAGGACGAAG ACAAAGCATC AGCAGCAAAG AAGAGTCGAG 
     2101 TTATCTCTGA TGCTGATGAT TCTGATAGTG ATGTTGTATC AGACAAATCA GGCAAAAGAG 
     2161 AGAAGACGGT AGCATCTGAT AGTGAAGAAG AAGTCGGGAA AGAAGAGTCT TCTGTAAAGA 
     2221 AGAGTGAAGA GAAGGATCTG TTTGGGAGTG ATAGTGAATC AGGCAATGAA GAAGAAAATC 
     2281 TTATTGCAGA TATATTTGGA GAATCTGGTG ATGAAGAGGA AGAGGAATTT ACAGGATTTA 
     2341 ACCAAGAAGA TTTGGAGGAA GAGAAAAATG AAACACAGCT AAAAGAAGCA GAAGATTCTG 
     2401 ATTCCGACGA TAACATAAAG AGAGGAAAGC ATATGGACTT TCTGTCAGAT TTTGAGATGA 
     2461 TGTTACAACG AAAAAAGAGC ATGTGTGGCA AACGCAGACG AAATCGAGAT GGGGGCACTT 
     2521 TTATTAGTGA CGCAGATGAT GTCGTGAGTG CCATGATTGT CAAGATGAAC GAAGCTGCTG 
     2581 AGGAAGACAG GCAATTGAAT AATCAAAAAA AGCCTGCTCT GAAAAAATTA ACCTTATTGC 
     2641 CTACTGTGGT TATGCACCTT AAAAAGCAGG ACCTTAAAGA AACATTTATT GACAGTGGTG 
     2701 TGATGTCTGC CATTAAAGAG TGGCTCTCAC CTCTACCAGA TAGGAGTTTG CCTGCACTGA 
     2761 AGATTCGGGA GGAATTGTTA AAGATTCTGC AAGAGCTACC TAGTGTGAGC CAAGAGACCC 
     2821 TGAAGCACAG TGGGATTGGC CGAGCAGTGA TGTATCTGTA TAAACATCCT AAGGAATCAA 
     2881 GGTCCAACAA GGACATGGCA GGGAAACTAA TCAATGAGTG GTCTCGGCCC ATATTTGGTC 
     2941 TTACCTCAAA CTATAAAGGA ATGACAAGAG AAGAAAGGGA GCAGAGAGAT CTAGAACAAA 
     3001 TGCCACAGCG ACGAAGAATG AGCAGCACTG GTGGTCAGAC ACCACGAAGA GACCTGGAAA 
     3061 AGGTACTAAC AGGAGAGGAA AAGGCTCTTA GACCTGGAGA TCCTGGCTTC TGTGCCCGTG 
     3121 CGAGAGTCCC AATGCCATCA AACAAGGACT ATGTTGTCAG GCCCAAGTGG AATGTAGAAA 
     3181 TGGAGTCATC CAGGCCTGGT ATTCTTAAAA AGGGTCTAAG TCGATTGGAA AAGCATAAGA 
     3241 GGCGGTTTGC AGAGCAGAAG AGGCTCAGCC AAATGCACCG TGCTGTCAAG TTCAGCATCG 
     3301 AAGGCAACAG AATGCCCCTG ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     3361 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     3421 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     3481 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     3541 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     3601 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     3661 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     3721 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     3781 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     3841 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     3901 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     3961 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     4021 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     4081 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     4141 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     4201 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     4261 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     4321 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     4381 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     4441 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     4501 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     4561 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     4621 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     4681 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     4741 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     4801 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     4861 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     4921 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     4981 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     5041 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     5101 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     5161 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     5221 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     5281 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     5341 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     5401 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     5461 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     5521 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     5581 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     5641 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     5701 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     5761 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     5821 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     5881 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     5941 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     6001 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     6061 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     6121 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     6181 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     6241 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     6301 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     6361 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     6421 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     6481 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     6541 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     6601 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     6661 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     6721 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     6781 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     6841 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     6901 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     6961 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     7021 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     7081 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     7141 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//