LOCUS       RR209577                8985 bp    DNA     circular  SYN 
DEFINITION  Pragmin (Myc-DDK-tagged ORF) - Rat pragma of Rnd2 (Pragmin), (10 ug)
ACCESSION   NM_001107315
VERSION     NM_001107315.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5142..5233 
                     /label="Myc-DDK"  
     misc_feature    5263..5848 
                     /label="PolyA signal"  
     misc_feature    5320..5339 
                     /label="Restriction Enzyme"  
     misc_feature    6124..6796 
                     /label="ColE1"  
     misc_feature    7226..8020 
                     /label="Kan/Neo"  
     misc_feature    8055..8357 
                     /label="SV40 ori"  
     misc_feature    8443..8920 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5124..5130 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5124 
                     /label="Prag1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCTGCGTGC AGTGACTTTG TGGAGCACAT CTGGAAACCC GGATCCTGCA 
     1081 AGAACTGCTT CTGCTTGAGG AGTGACCATC AGCTCACAGC TGGCCACCCC AAAGCTAGAG 
     1141 CCAGCAGCCT ACCCGCTGGA GCTCGCCTGC CTGCCAGGCC TGAGATTTGC CGCTTAGAGG 
     1201 ATGAAGGTGT GAATGGCTTG GCCTACTCAA AGCCCACCAT TGCGGTAAAG CCCACCATGA 
     1261 TGACTTCTGA GACTGCCGAC TTGTGGACTG AGGCCAGCCT GAGCGCTGAA GTTCCAAAGG 
     1321 TCAACTGGAG ACGAACTCCA GGCAAGCTCC TGCTCCAGAA ACAGGAAGAT GGGCCAATAG 
     1381 TTTACCTCGG CAGCTTCCGA GGAATGCAGA AGGCTGCAGG CCCTCTCGCC TGCACAGATA 
     1441 GCAACTCTCG TTGTCCCCCA GCCTACACCA TGGTCGGACT GCATAACCTG GAGGCCCGAG 
     1501 TGGATAGGAA CACCGCCCTC CAACCAGTGA ATTTCCAGGA AGAGAAGGCT GGGAGGGAAG 
     1561 AGCTGCCCTC AGCTCAGGAG AGCTTTCGTC AGAAGCTAGC TGCCTTTACA GGGATGACGT 
     1621 CCAGCTGCCT CAAGGGCCCC AGACCCTGCA CTTCTCCACA GCCCTTGCGG GAGTCCCTGC 
     1681 CCTCAGAGGA TGACAGTGAC CAAAGGTGCT CACCCTCAGG AGACAGCGAA GGTGGGGAGT 
     1741 ACTGCTCCAT TTTGGACTGT CGTCCAGAAT CCAGAGATGC TGTGCATAAC ACCGAGGGCT 
     1801 CTGGCCGACG GCGAGGGGAC TGCTCACCCA TATGCTGGGA GCAGGGAACA TGTACAAGAC 
     1861 CAACAGAAGA GGAAAAGCAG GCTCTGAACT TCCCCAGAGA GTGTTGTGGT CAGGGATCCA 
     1921 CAGCAAACCC ACCCCACCTG GGCCCCAAGA AGCCGTCCCT TAACTCAGAG GCTGCCAGCT 
     1981 CTTCAGATGG TCTGTCCTGT GGCAGCAGCC GCAGTGGTGC CAACAGCCCC TTTGCTCCAC 
     2041 ACCTGGAGAA TGACTACTGC TCTCTTGTGA AGGAGCCAAC CTCGGTGAAG CAGCAGGACT 
     2101 CTGGCTGCCA CTTAGTCAAC TCTGGCAAAT ATGTGGGTCA AGCCGTGGAC CTCCAGCCCC 
     2161 CAGCCCTTCC TAGGGAGGCT GTGCAGCCTG AACCCATCTA CGCCGAAAGT GCCAAGAGGA 
     2221 AGAAGGCAGC TCCGGTGCCT CAGAGGCCCG AGCCCAAGAA AGAGCAGGTC TCGTCTGGCC 
     2281 AAGTATGGAC AGGCGATACC TGGTCTCAGA AGACACCCTC TGGCTGGAGC CAGGAAAGGG 
     2341 AAGGCCCAAA CGCTGCTCCT CAGGTGGCAA CCACTATTAC TGTTATTGCT GCTCACCCAG 
     2401 AAGAGGACCA TAGGACTATT TATCTGAGCA GCCCAGACTC TGCCGTGGGG GTGCAGTGGC 
     2461 CACGTGGGTC TTTGAACCAG GACTTACATG GTAGTGGAGA AGAGCCTTTG GTCGTGCAGG 
     2521 GGCTAAGCTC AAGGGAGAGC CATCCACATA ATATGACAGA AAACTCGTCC AAAGAAAAGC 
     2581 CTGCCATACC CCCCAAGCTG TCCAAAAGCA GCCCGGGAGG GTCCCCGGTG TCACCTGCAG 
     2641 CCCCCCCTCT GACTGACCAC AGTGATGGGA ACACCAGTGG CAGCAGTGTC GGATCTCAGC 
     2701 CTTCGTCTAG AGTCCCTACT AACCTAACTT CTTCCTGCCA GACCAATGGT GTTGCCGCCG 
     2761 GTGACCCTGC CAAGTGTCCC CCACAAGCCA ACTCTTCAGT CTTGGACCAG AGGCGGCCAA 
     2821 GATACCAGAC TGGTGCATGG AGCCGCCAGT GCCGGATAGA GGAGGAGGAA GAGGTGGGGC 
     2881 AGGAGTTGTT GAGTCAAAGT TGGGGGAGAG AGTTGGAAAA TGGTATTGCA GACCATTCAA 
     2941 ACTCCTCTAC CTGGCACCGG CTCCATCCCA TTGATGGCGC CTCTGGGCAG AACGGCAAAA 
     3001 CTAACTCTGG GATGAGCAAA TCAGCTTCCT TTGCGTTTGA GTTCCCAAAG GACAGAGGCA 
     3061 GATTGGAGTC ATTCTCACCC CCTCCCCCAC CTCCAAAGTC AAGGCACCTG TTAAAAATGA 
     3121 ACAAGAGCAG CTCTGACTTG GAGAAAGTGA GCCAGAGCTC TGCAGAAAGC CTCAGCCCGT 
     3181 CCTTCCGAGG GGCCCACGTC AGCTTCACCA CGGGCTCCAC AGATAGCCTA GCCTCGGACT 
     3241 CCAGGACTTG CAGCGATGGA GGTCCATCGT GTGAAGCCAC CCACTCACCC ACCATCAGCG 
     3301 GGAAAAAGCT CTTTGCTCCC GTCCCTTTCC CGTCAGGCTC CACGGAGGAT GTGTCCCCCA 
     3361 GCGGTCCTGC ACAGCCTCCT CCTCTGCCCC AGAAAAAGAT AGTGAGCCGT GCTGCCTCCT 
     3421 CCCCTGATGG CTTCTTCTGG ACCCAAGGTT CCCCTAAGCC ACGCACAGCG AGTCCCAAGC 
     3481 TGAACCTAAG CCACTCGGAA ACCAATGTTT GTGCCCATGA CGAGCCTCCT TTGAGCTATT 
     3541 CCTTGAACTC TGGAAACCAT CCCCACCATG TCTTCTCCTC TTCTGAGCCT TTGGAGAAAG 
     3601 CTTTCAAAGG CAGTGTCCCC TGGGCCCCAG CTCTGGGTCC AGCAAACAGC AAAGGCGGTT 
     3661 GTGGGAGCCC CAATCTCCAG GGCAGAGCGG CCACCTCCAC CTCGTCATCC CAGCTCAGCG 
     3721 TGTCCAGCCA GGCCTCCACC GGCAGCAGCC AGCTTCAGCT GCACAGCCTG CTGAGCAGTA 
     3781 TCAGCAGCAA GGAGGGCACC TATGCCAAGC TCGGGGGCCT CTACACACAG TCCCTGGCCC 
     3841 GCCTAGTGAC CAAGTGCGAA GACCTCTTCA TGGGTGGTCT GAAAACGGAA CTGCGCTTCG 
     3901 ATGAGAACAG CTGGTCGCTC TTCAAACTGA TATGCAACAA GCCTTGCTGT GACTCGGGAG 
     3961 ATGCAATTTA CTACGGTGCC ACTTGCTCCA AGGACCCCGA CAGCATCTAC GCAGTGAAAA 
     4021 TCTGCAAAAC CCCAGAACCC AAGTCAGCTT CGTACTGTAG CCCGTCCGTG CCAGTGCACT 
     4081 TCAACATCCA GCAAGACTGC GGCCACTTTG TGGCCTCTGT GCCCTCCAGC ATGCTTGCCT 
     4141 TCCCCGACAC ATCCAGCAAG GACCCTGCGC CTGCTGCGCC CTCACACACC CCTGCCCAGG 
     4201 AGCAGGACTG TGTGGTGGTC ATCACCCGGG AGGTGCCGCA CCAGACTGCC TCGGACTTTG 
     4261 TGCGGGACTC TGTGGCCAGC CACCGCGCAG AGCCTGAGGT TTACGAGCGC CGGGTGTGCT 
     4321 TCTTGCTTCT GCAGCTCTGC AACGGGCTGG AGCATCTGAA GGAACATGGG ATCATCCACC 
     4381 GGGACCTATG TCTGGAGAAC CTGTTGCTAG TGCATTGCAA CCCTCAGAGC TCTCCCGGGC 
     4441 CCTCTGCTAA TCCCAGCGTG CCCACCACCA CGTCCCGTTG TCCTTCTGCC GCACCCGCAG 
     4501 CCACCACAGC CTGCCAGGGA GGACCTGGTG AGAAGCACCT GCCCAGGCTG ATCATCAGCA 
     4561 ACTTCCTGAA GGCCAAGCAG AAGCCGGGAG GCACCACCAA CCTGCAACAG AAGAAGAGCC 
     4621 AGGCCCGGCT GGCCCCTGAG ATCGTATCTG CCTCTCAGTA CCGTAAGTTT GATGAGTTCC 
     4681 AGACTGGCAT CCTCATCTAC GAGCTGCTCC ACCAGCCCAA CCCATTCGAG GTGCGAGCCC 
     4741 AGCTACGGGA GCGTGACTAC CGGCGGGAGG ACCTACCCCC GCTGCCCACA CTGTCGCTCT 
     4801 ACTCGCCCGG CCTGCAGCAG CTCGCCCACC TGCTCCTTGA GGCCGATCCC ATCAAGCGCA 
     4861 TCCGCATAGG TGAGGCCAAG CGGGTGCTAC AGTGCTTGCT GTGGGGGCCA CGGCGAGAGC 
     4921 TGGTAGAGCA GCCGTGCCCC TCGGAGGAGG TACTGTGCAA CACTCTGCAC AACTGGATCG 
     4981 ACATGAAGCG AGCCCTGATG ATGATGAAGT TTGCCGAGAA GGCGGTAGAA CGAAGGCGGG 
     5041 GTGTGGAGCT GGAGGATTGG CTCTGTTGCC AGTACTTGGC CTCAGCAGAG CCAGGGGCCC 
     5101 TCTTGCAGTC CCTGAAGCTC CTGCAACTTC TCACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     5161 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     5221 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     5281 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     5341 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     5401 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     5461 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     5521 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     5581 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5641 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5701 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5761 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5821 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5881 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5941 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     6001 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     6061 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     6121 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     6181 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     6241 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     6301 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     6361 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     6421 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     6481 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     6541 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     6601 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6661 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6721 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6781 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6841 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6901 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6961 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     7021 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     7081 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     7141 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     7201 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     7261 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     7321 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     7381 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     7441 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     7501 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     7561 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     7621 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7681 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7741 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7801 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7861 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7921 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7981 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     8041 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     8101 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     8161 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     8221 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8281 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8341 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     8401 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     8461 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     8521 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     8581 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     8641 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8701 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8761 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8821 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8881 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8941 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//