LOCUS       RR209547                7896 bp    DNA     circular  SYN 
DEFINITION  Grin3b (Myc-DDK-tagged ORF) - Rat glutamate receptor, ionotropic, N-methyl-D-aspartate 3B (Grin3b), (10 ug)
ACCESSION   NM_133308
VERSION     NM_133308.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4051..4142 
                     /label="Myc-DDK"  
     misc_feature    4172..4757 
                     /label="PolyA signal"  
     misc_feature    4229..4248 
                     /label="Restriction Enzyme"  
     misc_feature    5033..5705 
                     /label="ColE1"  
     misc_feature    6135..6929 
                     /label="Kan/Neo"  
     misc_feature    6964..7266 
                     /label="SV40 ori"  
     misc_feature    7352..7829 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4026..4033 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4026 
                     /label="Grin3b"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGAGTGTG CGGACGCTGT GGCTCAGCGT GGCCCTGGCG CTGGCGGTGG 
     1081 GGTCCCGAGT GGTGCGCGGT CACCCTCAGC CCTGCCGGGT TCCCACGCGC GCTGGGGCCT 
     1141 CCGTGCGCCT GGCGGCGCTC CTGCCCCGGG CGCCCGCCGC CCGCGCCCGC GTCCTAGCTG 
     1201 CCCTGGCCAC CCCTGCGCCG CGGCTGCCGC ACAACCTGAG TCTGGAACTG GTGGCCGTCG 
     1261 CGTCCCCGAC CCGGGACCCC GCGTCGCTAG CTCGAGGTCT GTGCCAGGTT CTGGCACCGC 
     1321 CTGGCGTGGT GGCCTCTATA GCCTTTCCCG AGGCGCGGCC CGAGCTGCGG CTACTGCAGT 
     1381 TCCTGGCAGC CGCCACAGAG ACCCCAGTGG TGAGCGTCCT GCGGAGGGAG GTGCGCACGG 
     1441 CCCTCGGAGC CCCGACTCCG TTCCATCTGC AGCTGGACTG GGCTAGTCCC CTGGAGACCA 
     1501 TACTGGATGT GCTGGTGTCC CTGGTACGGG CACATGCCTG GGAGGACATT GCTCTAGTAC 
     1561 TCTGCCGTGT CCGGGACCCT GGCAGCCTGG TGACACTCTG GACTAACCAT GCTAGCCAGG 
     1621 CTCCAAAGTT TGTGCTGGAC CTGAGCCGGC TGGACAGCAG GAATGACAGC CTTCGGGCTG 
     1681 GACTGGCCCT GTTGGGGGCG CTGGAAGGAG GGGGAACCCC AGTGCCTGCA GCAGTCCTCC 
     1741 TAGGCTGCAG CACTGCCCGT GCACATGAGG TCCTAGAGGC TGCACCACCG GGTCCCCAGT 
     1801 GGTTGCTGGG CACACCATTG CCCGCTGAGG CACTGCCCAC GACTGGTCTG CCACCTGGCG 
     1861 TGCTGGCGCT GGGGGAAACC GAACAACACT CTCTGGAAGC TGTCGTCCAC GACATGGTGG 
     1921 AGCTTGTGGC TCAGGCACTC AGTAGCATGG CCCTTGTACA CCCAGAGCGG GCACTGCTTC 
     1981 CAGCTGTGGT GAACTGTGAT GACCTGAAAA CAGGCGGATC TGAGGCAACA GGGCGCACCT 
     2041 TGGCTCGGTT TCTCGGCAAC ACCTCATTTC AGGGCCGAAC AGGGGCCGTG TGGGTGACAG 
     2101 GCTCCTCTCA GGTGCATGTG TCTCGGCATT TCAAGGTATG GAGCCTGCGC CGGGATCCGC 
     2161 TGGGTGCCCC AGCCTGGGCA ACCGTGGGCA GCTGGCAGGA TGGACAGCTG GACTTCCAGC 
     2221 CAGGGGCAGC CGCTCTCCGA GTCCCATCTC CGTCTGGCAC CCAGGCCCGA CCAAAGCTGC 
     2281 GTGTGGTAAC CCTGGTGGAA CACCCGTTTG TGTTCACCAG GGAATCTGAT GAAGACGGAC 
     2341 AGTGCCCAGC TGGGCAGCTG TGTCTGGACC CAGGCACCAA TGACTCAGCC AGGCTGGATG 
     2401 CCCTCTTTGC TGCACTGGTG AATGGCTCAG TACCTCGAAC GCTGAGAAGA TGCTGCTATG 
     2461 GCTACTGCAT CGACCTGCTG GAGCGGCTGG CCGAGGACCT GGCCTTTGAC TTTGAGCTCT 
     2521 ATATTGTGGG GGATGGCAAG TACGGGGCCC TGCGTGATGG GCGCTGGACG GGCCTGGTGG 
     2581 GTGACCTGCT GGCTGGCCGG GCACACATGG CTGTGACCAG CTTCAGCATC AACTCAGCTC 
     2641 GCTCTCAGGT GGTGGATTTC ACCAGCCCTT TCTTCTCCAC CAGCCTGGGG ATTATGGTGC 
     2701 GCACGAGAGA CACGGCCTCG CCCATCGGGG CTTTCATGTG GCCCCTGCAC TGGTCCATGT 
     2761 GGGTGGGCGT GTTTGCTGCT CTGCACCTCA CAGCGCTCTT TCTCACCCTG TACGAATGGC 
     2821 GAAGTCCCTA CGGGCTCACG CCGCGCGGCC GCAACCGTGG CACTGTCTTC TCTTACTCCT 
     2881 CCGCGCTCAA CCTGTGCTAT GCCATTCTCT TTGGACGCAC TGTCTCCAGT AAGACGCCCA 
     2941 AGTGCCCTAC TGGACGCTTC CTCATGAACC TCTGGGCAAT CTTCTGCCTG CTGGTGCTTT 
     3001 CCAGTTACAC GGCCAACCTG GCTGCTGTCA TGGTTGGGGA CAAAACCTTT GAGGAGCTGT 
     3061 CTGGAATCCA TGATCCCAAG CTGCACCACC CTTCCCAAGG CTTTCGCTTT GGCACCGTAT 
     3121 GGGAGAGCAG CGCGGAGGCC TACATCAAGG CAAGCTTCCC TGAGATGCAC GCACACATGC 
     3181 GTCGGCACAG CGCACCCACC ACTCCACATG GGGTGGCCAT GCTCACGAGC GACCCGCCCA 
     3241 AGCTCAACGC CTTCATCATG GATAAATCAC TACTGGACTA TGAGGTGTCC ATAGATGCGG 
     3301 ACTGCAAGCT GCTCACCGTT GGCAAACCCT TTGCTATCGA GGGCTACGGC ATAGGGCTAC 
     3361 CCCAAAACTC GCCGCTCACC TCCAACCTGT CGGAGTTCAT CAGTAGGTAC AAGTCTTCAG 
     3421 GCTTCATTGA TCTGCTCCAT GACAAGTGGT ACAAGATGGT GCCTTGCGGG AAGCGGGTGT 
     3481 TCGCCGTGAC GGAGACGCTG CAGATGGGGG TCTACCACTT CTCAGGATTG TTTGTCCTGC 
     3541 TGTGCCTCGG GCTGGGCAGC GCGCTTCTCA CCTCTCTGGG TGAGCATGTC TTCTACCGCC 
     3601 TGGTGCTGCC GCGCATCCGC AGGGGTAATA AGCTGCAGTA TTGGCTTCAC ACGAGCCAGA 
     3661 AGATCCACCG AGCCCTCAAT ACAGGACCAC CCGAGGGGCA ACAGGAGAGG GCAGAGCAGG 
     3721 AGCGCAGCGG CCCCAAGGAC GAGCTGCCTG CCACCGATGG TGCAGGGCGC TGGAGGCGGG 
     3781 TGCGCCGGGC TGTGGAACGG GAGCGACGCG TGCGTTTCCT GCTGGAACCT GGGGAGGCTG 
     3841 GCGGAGACCG CCCGTGGCTC TGCTCCAACG GGCCCGGGCT GCAAGCGGAG CTGCGGGAGC 
     3901 TGGAGCTGCG CATTGAGGCT GCACGGGAGC GGCTGCGCAG TGCGCTGTTG CGGCGCGGGG 
     3961 AGCTGCGGGC CCTGCTTGGG GATGGCACCC GGCTCAGGCC ACTGCGCCTG TTGCATGCGG 
     4021 CGCCTGCTGA GAGCAGCGGA CCGACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4081 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4141 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4201 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4261 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4321 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4381 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4441 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4501 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4561 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4621 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     4681 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     4741 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     4801 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     4861 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     4921 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     4981 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5041 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5101 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5161 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5221 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5281 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5341 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     5401 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     5461 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     5521 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     5581 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     5641 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     5701 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     5761 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     5821 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     5881 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     5941 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6001 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6061 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6121 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6181 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6241 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6301 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6361 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     6421 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     6481 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     6541 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     6601 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     6661 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     6721 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     6781 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     6841 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     6901 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     6961 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7021 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7081 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7141 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7201 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7261 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7321 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7381 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     7441 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     7501 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     7561 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     7621 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     7681 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     7741 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     7801 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     7861 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//