LOCUS       RR209512                6193 bp    DNA     circular  SYN 
DEFINITION  Npffr2 (Myc-DDK-tagged ORF) - Rat neuropeptide FF receptor 2 (Npffr2), (10 ug)
ACCESSION   NM_023980
VERSION     NM_023980.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2350..2441 
                     /label="Myc-DDK"  
     misc_feature    2471..3056 
                     /label="PolyA signal"  
     misc_feature    2528..2547 
                     /label="Restriction Enzyme"  
     misc_feature    3332..4004 
                     /label="ColE1"  
     misc_feature    4434..5228 
                     /label="Kan/Neo"  
     misc_feature    5263..5565 
                     /label="SV40 ori"  
     misc_feature    5651..6128 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2332..2338 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2332 
                     /label="Npffr2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCAAGAGA TGGGACTCAA ACTCTTCAGG AAGCTGGGAT CACATCTGGA 
     1081 GTGGCAATGA CACACAGCAT CCTTGGTATT CAGATATCAA CATCACATAC ATGAACTACT 
     1141 ATCTCCACCA GCCCCACGTG ACAGCTGTCT TCATTAGCTC CTACTTCCTG ATCTTCTTCC 
     1201 TGTGCATGGT GGGAAACACT GTCGTTTGCT TTGTTGTAAT AAGGAATAGG TACATGCACA 
     1261 CGGTCACTAA TTTCTTCATC TTCAACCTCG CAATAAGTGA CTTACTGGTT GGAATATTCT 
     1321 GCATGCCTAT CACATTGCTG GACAACATCA TAGCAGGATG GCCGTTTGGA AGCAGCATGT 
     1381 GCAAGATCAG CGGGCTGGTG CAAGGGATAT CGGTTGCCGC TTCTGTCTTC ACCTTGGTTG 
     1441 CCATAGCCGT AGACAGATTC CGGTGTGTGG TCTACCCCTT TAAGCCCAAG CTCACTGTCA 
     1501 AGACAGCCTT TGTCATGATC GTGATCATCT GGGGCCTGGC CATCACCATT ATGACCCCAT 
     1561 CTGCAATCAT GTTACATGTA CAGGAAGAAA AATACTACCG TGTGAGGCTC AGCTCCCACA 
     1621 ATAAAACCAG CACAGTCTAC TGGTGTCGGG AGGATTGGCC AAACCAGGAA ATGAGGAGGA 
     1681 TCTACACCAC CGTGCTCTTT GCCACTATCT ACCTGGCTCC ACTCTCCCTC ATTGTTATCA 
     1741 TGTATGCAAG GATTGGGGCT TCCCTCTTCA AGACCTCAGC ACACAGCACA GGTAAGCAGC 
     1801 GCCTGGAGCA GTGGCATGTA TCCAAGAAGA AACAGAAGGT CATCAAGATG CTGCTGACTG 
     1861 TGGCCCTCCT TTTCATCCTT TCCTGGCTTC CCCTGTGGAC TCTGATGATG CTCTCAGACT 
     1921 ATGCTGACCT GTCACCTAAC AAACTACGTG TCATCAATAT TTATGTCTAC CCTTTTGCCC 
     1981 ACTGGCTCGC CTTCTGCAAT AGCAGTGTCA ACCCCATCAT TTATGGTTTC TTTAATGAAA 
     2041 ATTTTCGCAG TGGTTTCCAA GATGCTTTCC AGTTCTGCCA AAAGAAAGTC AAACCCCAGG 
     2101 AAGCCTATGG CCTAAGAGCT AAACGCAACC TGGACATAAA CACATCTGGC CTGTTGGTCC 
     2161 ATGAACCTGC ATCTCAAAAC CCAAGTGGGG AAAACTTGGG ATGTAGAAAA AGTGCAGACA 
     2221 ATCCCACACA GGAATCCTTG ATGGAGGAAA CGGGAGAAGC TACCAACAGT ACTGAGACTC 
     2281 AATCCCACAC AGGAATCCTT GATGGAGGAA ACGGGAGAAG CTACCAACAG TACTGAGACT 
     2341 ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT 
     2401 ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT 
     2461 TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT 
     2521 GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT 
     2581 GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG 
     2641 GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC 
     2701 AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC 
     2761 TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT 
     2821 TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA 
     2881 GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC 
     2941 CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA 
     3001 TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT 
     3061 CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG 
     3121 CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG 
     3181 GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC 
     3241 TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC 
     3301 AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC 
     3361 TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT 
     3421 CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG 
     3481 TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT 
     3541 CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG 
     3601 CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT 
     3661 GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC 
     3721 CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA 
     3781 GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG 
     3841 ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA 
     3901 TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA 
     3961 GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC 
     4021 CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG 
     4081 AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC 
     4141 CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC 
     4201 TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA 
     4261 GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC 
     4321 AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT 
     4381 CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC 
     4441 CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC 
     4501 GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC 
     4561 ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT 
     4621 GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT 
     4681 CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG 
     4741 CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG 
     4801 AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC 
     4861 AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG 
     4921 GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC 
     4981 TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG 
     5041 CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG 
     5101 AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG 
     5161 TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA 
     5221 TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA 
     5281 AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG 
     5341 CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC 
     5401 TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT 
     5461 TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG 
     5521 TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT 
     5581 TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT 
     5641 TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA 
     5701 ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC 
     5761 TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC 
     5821 CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC 
     5881 CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT 
     5941 TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG 
     6001 GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG 
     6061 TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT 
     6121 ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT 
     6181 TAACGCGAAT TTT
//