LOCUS       RR209498                8331 bp    DNA     circular  SYN 
DEFINITION  Aff4 (Myc-DDK-tagged ORF) - Rat AF4/FMR2 family, member 4 (Aff4), (10 ug)
ACCESSION   NM_001107001
VERSION     NM_001107001.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4488..4579 
                     /label="Myc-DDK"  
     misc_feature    4609..5194 
                     /label="PolyA signal"  
     misc_feature    4666..4685 
                     /label="Restriction Enzyme"  
     misc_feature    5470..6142 
                     /label="ColE1"  
     misc_feature    6572..7366 
                     /label="Kan/Neo"  
     misc_feature    7401..7703 
                     /label="SV40 ori"  
     misc_feature    7789..8266 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4470..4476 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4470 
                     /label="Aff4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAACCGTGAA GACCGGAATG TGCTGCGCAT GAAAGAAAGG GAAAGGCGGA 
     1081 ATCAGGAAAT TCAGCAGGGT GAAGACGCCT TCCCACCTAG TTCTCCTCTC TTTGCTGAGC 
     1141 CGTACAAAGT TACTAGCAAA GAAGATAAGT TATCAAGTCG TATTCAGAGT ATGCTTGGAA 
     1201 ACTACGATGA AATGAAGGAT TACATAGGAG ACAGATCTAT ACCAAAGCTT GTTGCAATTC 
     1261 CCAAGCCTGC AGTACCGACG ACAACAGACG AGAAAGCAAA CCCAAATTTT TTTGAACAGA 
     1321 GACATGGAGG ATCTCATCAG AGTAGCAAGT GGACTCCTGT AGGACCTGCA CCCAGCACCT 
     1381 CTCAGTCTCA GAAGCGCTCC TCTGGCTTGC AGAGTGGACA CAGTAGCCAG CGGAGCAGTG 
     1441 CAGGTGGGAG TGGCGCTAGT GGTAGTGGCC AGAGGCATGA CCGTGACTCC TACAGCAGTA 
     1501 GCAGGAAAAA AGGCCAGCAC GGGTCAGAGC ACTCCAAATC ACGATCCTCA AGCCCTGGAA 
     1561 AACCCCAGGC TGTTTCTTCA CTAAGTTCTA GTCATTCCAG GTCTCATGGG AATGACCACC 
     1621 ATAGCAAGGA ACATCAGCGC TCCAAATCAC CTCGGGACCC TGATGCCAAC TGGGATTCTC 
     1681 CTTCCCGGGT ACCTTTTTCA AGTGGGCAAC ATTCAAGTCA GTCTTTCCCA CCTTCTTTGA 
     1741 TGTCAAAATC TAGTTCAATG TTACAGAAAC CCACTGCCTA TGTACGGCCC ATGGATGGAC 
     1801 AGGAGTCTGT GGAACCAAAG CTGTCCTCTG AGCACTACAG CAGCCAGTCT CATGGTAGTA 
     1861 ACATGACTGA GCTGAAGCCC AGCAGCAAAG CACATCTTAC CAAGCTGAAA ATACCTTCCC 
     1921 GACCTCTGGA TGCATCAGTT TCTGGTGATG TAAGCTGTGT GGATGAAATA CTTAAAGAGA 
     1981 TGACGCACTC ATGGCCTCCC CCTCTAACGG CTATTCATAC ACCATGCAAA ACAGAACCTT 
     2041 CCAAATTTCC TTTTCCAACT AAGGAGTCTC AGCAGTCTAA CTTTGGCTCT GGAGAACAAA 
     2101 AAAGATACAG TACTTCTGCT AAAACATCAA ATGGACACCA GTCAAAATCT ATGTTAAAAG 
     2161 ATGACTTAAA ACTAAGCAGC AGTGAAGACA GTGATGGGGA ACAGGATTGT GATAAGACAA 
     2221 TGCCAAGGAG TACACCAGGA AGTGAGGGAG CAGATAACTC CAGGGATGAC TCAAGCAGCC 
     2281 ACAGTGGGTC TGAAAGCAGC TCTGGGTCCG ACTCCGAGAG TGAAAGCAGC TCCAGTGACA 
     2341 GTGAGGCAAA TGAGCCATCC CAGAGCGCAT CCCCTGAGCC TGAACCACCA CCAACCAACA 
     2401 AGTGGCAACT TGATAATTGG TTGAACAAAG TGAACCCACA TAAAGTGTCC CCAGCTTCCT 
     2461 CTGTAGATAG CAACATCCCA TCGTCTCAAG CCTACAAGAA AGAAGGCCGA GAGCAGGGCA 
     2521 CTGCCAGTAA CTACACAGAT CCAGGTGGGG CCAAAGAAGC GAGCTCTGCC ACTCCGGGAC 
     2581 GAGACTCTAA AACTATCCAA AAAGGATCAG AAAGTGGCCG TGGGAGGCAG AAGTCTCCTG 
     2641 CACAGAGTGA CAGTACCACA CAGAGGAGAA CTGTTGGCAA AAAACAACCC AAAAAAACTG 
     2701 AGAAGTCAGC TGCTGAAGAG CCCCGAGGAG GCCTGAAGAT AGAAAGTGAG ACCCCTGTGG 
     2761 ACATGGCTAC CAGCATGCCC TCCAGCAGGC ATAAGGCAGC CACCAAGGGC TCAAGGAAAC 
     2821 CTAACATCAA AAAGGAGTCG AAATCTTCCC CTCGACCTAC AGCAGAGAAA AAGAAATACA 
     2881 AGTCAGCAAG TAAATCCTCC CAGAAATCTC GGGAAATCAT AGAAACAGAT AGCTCCTCCT 
     2941 CAGATTCAGA TGAGAGGGAG AGCCTCCCTC CCTCCTCCCA GACTCCTAAG TACCCTGAGA 
     3001 GCAATAGGAC TCCTGTCAAA CCCTCCTCGG CGGAGGAAGA CGATAGCTTT TTCCGGCAGC 
     3061 GGATGTTCTC TCCTATGGAA GAGAAGGAGC TCCTTTCCCC ACTCAGTGAC CCTGATGACA 
     3121 GGTACCCACT CATTGTGAAG ATAGACCTGA ATCTTTTGAC TAGAATACCC GGGAAGCCTT 
     3181 ACAAAGAAAC AGAGCCACCC AAGGGGGAAA AGAAAAATGT TCCAGAAAAA CACTCAAGAG 
     3241 AGGTGCAGAA GCAAGCCTCA GAAAAGGCTT CCAACAAAGG CAAGAGGAAA CACAAGAATG 
     3301 ATGATGATAA CCGAGCCAGT GAGAGCAAAA AACCCAAGAC GGAGGACAAG AACTCATCAG 
     3361 CCCACAAGCC ATCCAGCAGC AAAGAGTCAT CGAAGCAGAG TAGTACAAAA GAAAAGGACT 
     3421 TGCTACCTTC ACCTGCTGGG CCTGTTCCTT CAAAAGATTC AAAAACAGAG CATGGCTCTC 
     3481 GGAAGAGGAC TGTCAGTCAG TCATCATCCT TAAAGTCCAG CAGTACCAGC AACAAAGAGA 
     3541 ACAGTGGCAG CAGCAAGAGC ACTTCCTCCA CAGCAAAACA GAAGAAGACT GAAGGGAAGG 
     3601 TTTCCAGCAG CTCCAAAGAG GCCAAGGAGA AGGCTCCAAA TAGCTCCTCT AATTGTCCTC 
     3661 CGTCTACACC AACTCCTGAT TCTTCGAAGC CTCGAAGAAC GAAGCTCGCC TTTGATGACA 
     3721 GAAATTATTC AGCAGACCAT TATTTACAAG AAGCAAAAAA ACTCAAACAC AATGCAGATG 
     3781 CTTTGTCTGA TAGGTTTGAG AAAGCTGTGT ACTACCTTGA TGCCGTAGTG TCTTTCATTG 
     3841 AATGTGGGAA TGCATTAGAG AAGAACGCTC AGGAATCCAA GTCTCCATTC CCTATGTACT 
     3901 CAGACACAGT GGAGCTTATT AAATACACTA TGAAACTAAA GAATTATTTG GCGCCAGATG 
     3961 CTACAGCTGC AGACAAGAGA CTCACAGTAC TTTGCCTGAG ATGCCAGTCA TTGTTGTACC 
     4021 TGAGGCTGTT CAAACTGAAG AAGGAGAACG CTCTGAAGTA CTCCAAGACG CTGACTGAGC 
     4081 ACCTGAAGAA TTCTTACAGT AATTCTCAAG CACCTTCACC TGGCTTGGGG AGCAAAGCTG 
     4141 TTGGGATGCC CTCCCCTGTT TCTCCAAAGC TGTCACCAGG CAATTCTGGA AGTTACTCTT 
     4201 CTGGGGGAAG TAGTGCTTCA GCAAGTGGTT CTTCGGTGAC CATTCCACAG AAAATCCACC 
     4261 AGATGGCAGC AAGCTATGTC CAGGTTACAT CTAACTTCCT CTATGCCACA GAAATTTGGG 
     4321 ACCAAGCTGA ACAGCTTTCC AAAGAACAAA AAGAATTCTT TGCTGAACTG GACAAAGTGA 
     4381 TGGGCCCTCT CATCTTTAAT GCAAGCATCA TGACAGATCT GGCTCGTTAC ACCCGGCAGG 
     4441 GACTGCATTG GCTTCGGCAG GATGCCAAGC TGATATCTAC GCGTACGCGG CCGCTCGAGC 
     4501 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4561 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4621 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4681 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4741 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4801 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4861 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4921 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     4981 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5041 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5101 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5161 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5221 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5281 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5341 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5401 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5461 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5521 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5581 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5641 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5701 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5761 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     5821 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     5881 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     5941 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6001 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6061 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6121 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6181 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6241 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6301 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6361 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6421 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6481 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6541 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6601 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6661 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6721 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6781 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     6841 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     6901 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     6961 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7021 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7081 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7141 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7201 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7261 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7321 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7381 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7441 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7501 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7561 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7621 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7681 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7741 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7801 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     7861 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     7921 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     7981 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8041 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8101 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8161 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8221 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8281 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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