LOCUS       RR209467                8667 bp    DNA     circular  SYN 
DEFINITION  Adcy5 (Myc-DDK-tagged ORF) - Rat adenylate cyclase 5 (Adcy5), (10 ug)
ACCESSION   NM_022600
VERSION     NM_022600.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4824..4915 
                     /label="Myc-DDK"  
     misc_feature    4945..5530 
                     /label="PolyA signal"  
     misc_feature    5002..5021 
                     /label="Restriction Enzyme"  
     misc_feature    5806..6478 
                     /label="ColE1"  
     misc_feature    6908..7702 
                     /label="Kan/Neo"  
     misc_feature    7737..8039 
                     /label="SV40 ori"  
     misc_feature    8125..8602 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4806..4812 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4806 
                     /label="Adcy5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCCGGCTCC AAAAGCGTGA GCCCCCCGGG CTACGCTGCA CAGACAGCAG 
     1081 CGTCGCCCGC GCCCCGGGGA GGCCCGGAGC ATCGCGCCGC GTGGGGAGAA GCCGATTCCC 
     1141 GCGCCAATGG CTACCCCCAC GCCCCCGGGG GATCGACCCG CGGCTCCACC AAGCGATCTG 
     1201 GGGGAGCGGT GACCCCACAA CAGCAGCAGC GCCTGGCTAG CCGTTGGCGC GGTGGCGACG 
     1261 ACGATGAAGA CCCTCCACTA AGCGGTGACG ACCCTCTGGT CGGAGGCTTC GGCTTCAGCT 
     1321 TCCGTTCTAA GTCGGCCTGG CAGGAGCGCG GCGGCGACGA CGGCGGTCGC GGCAGCAGGC 
     1381 GGCAGCGGCG GGGCGCGGCT GGAGGGGGCA GCACCCGGGC GCCCCCTGCG GGCGGCAGTG 
     1441 GCAGTTCGGC GGCGGCCGCA GCAGCCGCAG GTGGCACGGA GGTGCGCCCC CGCTCGGTGG 
     1501 AGGTGGGCCT GGAGGAGCGT CGAGGAAAAG GCCGAGCGGC CGAGGAGCTG GAGCCCGGAA 
     1561 CTGGCACCGT CGAGGATGGA GACGGGTCCG AGGATGGAGG CAGTTCCGTG GCGTCAGGCT 
     1621 CTGGGACCGG CACGGTGCTG TCGTTGGGCG CCTGCTGCCT GGCCTTGCTG CAGATATTCC 
     1681 GCTCTAAGAA GTTCCCGTCG GACAAACTGG AGCGTCTGTA CCAGCGCTAT TTCTTCCGCC 
     1741 TGAACCAGAG CAGCCTCACC ATGCTCATGG CCGTGCTGGT GCTCGTGTGC CTGGTCATGC 
     1801 TCGCTTTCCA CGCGGCACGG CCCCCGCTCC AGGTAGTCTA CCTGGCCGTG TTGGCAGCTG 
     1861 CTGTGGGCGT GATCCTTATC ATGGCTGTGC TCTGCAACCG TGCAGCCTTC CACCAGGACC 
     1921 ACATGGGCCT GGCCTGCTAT GCGCTCATTG CAGTGGTGCT GGCCGTCCAG GTAGTGGGCC 
     1981 TGTTGCTGCC ACAGCCACGC AGCGCCTCCG AGGGCATCTG GTGGACCGTG TTCTTCATCT 
     2041 ATACCATCTA CACCCTGCTG CCTGTGCGCA TGAGGGCTGC GGTGCTCAGC GGGGTGCTTC 
     2101 TGTCGGCTCT CCACTTGGCC ATCTCTCTGC ACACCAACGC CCAGGACCAG TTTCTGCTGA 
     2161 AACAGCTTGT CTCCAACGTC CTCATCTTCT CTTGCACCAA CATCGTGGGT GTGTGCACTC 
     2221 ACTACCCAGC CGAGGTCTCC CAGAGACAAG CCTTCCAGGA GACCCGGGAG TGCATCCAAG 
     2281 CTCGGCTCCA CTCACAGCGG GAGAACCAGC AACAGGAGCG TCTCCTGCTG TCTGTCCTTC 
     2341 CCCGTCATGT TGCCATGGAG ATGAAAGCAG ACATCAACGC CAAACAGGAG GATATGATGT 
     2401 TCCACAAGAT TTACATCCAG AAACATGACA ATGTGAGCAT CCTGTTTGCT GACATCGAAG 
     2461 GCTTCACTAG CCTGGCATCC CAGTGTACTG CCCAAGAACT GGTCATGACC CTCAACGAGC 
     2521 TCTTCGCCCG CTTTGACAAG TTGGCTGCGG AGAATCACTG CTTACGGATT AAGATCCTCG 
     2581 GGGATTGTTA CTACTGTGTC TCGGGGCTGC CTGAAGCCAG AGCTGACCAC GCCCACTGCT 
     2641 GCGTAGAGAT GGGAATGGAC ATGATCGAGG CCATCTCGTC GGTCCGGGAG GTGACAGGGG 
     2701 TGAACGTGAA CATGCGTGTG GGAATTCACA GCGGGAGAGT ACACTGCGGT GTCCTTGGCC 
     2761 TCAGAAAGTG GCAATTCGAC GTGTGGTCTA ACGATGTCAC ACTGGCCAAC CACATGGAAG 
     2821 CTGGCGGCAA GGCAGGCCGC ATCCACATCA CCAAGGCCAC ACTCAACTAC CTGAACGGGG 
     2881 ACTATGAGGT GGAGCCAGGC TGTGGTGGTG AGCGCAATGC CTACCTCAAG GAGCACAGCA 
     2941 TCGAGACCTT CCTCATCCTG CGCTGTACCC AGAAGCGGAA AGAAGAGAAG GCCATGATCG 
     3001 CCAAGATGAA CCGCCAGAGA ACCAACTCCA TTGGACACAA TCCGCCTCAC TGGGGAGCTG 
     3061 AGCGCCCCTT CTACAACCAC TTGGGTGGCA ACCAGGTGTC CAAGGAAATG AAGAGGATGG 
     3121 GCTTTGAGGA CCCCAAGGAC AAGAATGCCC AGGAAAGTGC CAACCCTGAG GATGAAGTGG 
     3181 ACGAGTTTCT GGGTCGAGCC ATCGATGCCA GGAGTATTGA CAGACTGCGA TCCGAACACG 
     3241 TCCGAAAGTT CCTCTTGACC TTTAGGGAGC CCGACTTAGA GAAGAAGTAC TCCAAGCAGG 
     3301 TGGATGACCG ATTTGGTGCC TATGTGGCCT GTGCCTCGCT TGTTTTCCTC TTCATCTGCT 
     3361 TTGTCCAGAT CACCATTGTG CCCCACTCCC TGTTCATGCT GAGCTTCTAC CTGTCGTGTT 
     3421 TCCTGCTACT GGCCTTGGTG GTGTTTATAT CTGTGATCTA CGCCTGTGTG AAGCTCTTCC 
     3481 CTACTCCCCT GCAGACACTC TCCAGGAAGA TAGTGCGATC CAAGAAGAAC AGCACCCTGG 
     3541 TCGGGGTGTT CACCATCACC CTGGTGTTCC TCTCGGCTTT TGTCAACATG TTCATGTGCA 
     3601 ACTCTAAGAA CCTGGTGGGT TGCCTGGCAG AGGAGCACAA CATCACGGTG AACCAGGTGA 
     3661 ACGCATGTCA TGTGATGGAG TCGGCCTTCA ACTACAGCCT GGGCGACGAG CAGGGCTTCT 
     3721 GTGGCAGCCC CCAGTCCAAC TGCAACTTCC CAGAGTACTT CACCTACAGC GTGCTGCTCA 
     3781 GCCTGCTGGC CTGCTCCGTG TTCCTGCAGA TCAGCTGCAT CGGGAAGCTG GTGCTCATGC 
     3841 TGGCCATCGA GCTCATCTAC GTGCTCATCG TAGAGGTGCC CGGTGTCACA CTCTTTGACA 
     3901 ACGCTGACCT TCTGGTCACC GCCAATGCCA TAGACTTCAG CAACAACGGG ACCTCCCAGT 
     3961 GCCCTGAGCA TGCGACCAAG GTGGCGCTGA AGGTGGTGAC GCCCATCATC ATCTCTGTCT 
     4021 TCGTGCTGGC TCTGTATCTG CATGCTCAGC AGGTGGAATC CACTGCTCGC CTTGACTTCC 
     4081 TCTGGAAACT GCAGGCCACA GAAGAGAAGG AGGAGATGGA GGAGCTGCAG GCCTACAACC 
     4141 GGCGCTTGCT GCACAACATT CTGCCCAAGG ACGTGGCTGC CCACTTCCTG GCCCGGGAGC 
     4201 GGCGCAATGA TGAACTGTAC TACCAATCCT GCGAGTGCGT GGCTGTCATG TTTGCCTCCA 
     4261 TCGCCAACTT CTCCGAATTC TACGTGGAGC TAGAGGCCAA CAATGAGGGC GTTGAATGCC 
     4321 TACGGCTGCT CAATGAGATC ATCGCAGACT TTGATGAGAT CATCAGTGAG GATCGGTTCA 
     4381 GGCAGCTGGA AAAGATCAAG ACCATAGGTA GCACCTACAT GGCTGCCTCT GGCCTCAACG 
     4441 ACTCCACCTA TGACAAGGCA GGCAAGACCC ACATCAAGGC TCTTGCAGAC TTCGCCATGA 
     4501 AGCTGATGGA CCAAATGAAG TACATCAATG AGCACTCCTT CAACAACTTC CAGATGAAGA 
     4561 TCGGGCTTAA CATTGGACCT GTAGTGGCTG GGGTCATTGG GGCTCGCAAG CCTCAGTATG 
     4621 ACATCTGGGG CAATACAGTA AATGTGGCCA GCCGTATGGA CAGCACTGGG GTGCCTGACC 
     4681 GCATCCAGGT TACTACAGAT ATGTACCAGG TGCTGGCCGC CAACACATAC CAGCTGGAGT 
     4741 GCCGGGGTGT GGTCAAGGTC AAGGGCAAGG GTGAGATGAT GACCTACTTC CTCAATGGAG 
     4801 GGCCTCCCCT CAGTACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4861 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     4921 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4981 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     5041 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     5101 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     5161 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     5221 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     5281 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     5341 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     5401 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5461 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5521 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5581 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     5641 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5701 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5761 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     5821 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     5881 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     5941 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     6001 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     6061 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     6121 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     6181 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     6241 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     6301 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     6361 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     6421 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6481 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6541 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6601 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6661 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6721 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6781 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6841 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     6901 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6961 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     7021 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     7081 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     7141 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     7201 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     7261 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     7321 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     7381 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     7441 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7501 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7561 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7621 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7681 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7741 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7801 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7861 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     7921 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7981 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     8041 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     8101 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     8161 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     8221 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     8281 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     8341 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     8401 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     8461 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     8521 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     8581 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     8641 ATTTAACAAA AATTTAACGC GAATTTT
//