LOCUS       RR209443                8841 bp    DNA     circular  SYN 
DEFINITION  Spag9 (Myc-DDK-tagged ORF) - Rat sperm associated antigen 9 (Spag9), (10 ug)
ACCESSION   NM_001108290
VERSION     NM_001108290.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4998..5089 
                     /label="Myc-DDK"  
     misc_feature    5119..5704 
                     /label="PolyA signal"  
     misc_feature    5176..5195 
                     /label="Restriction Enzyme"  
     misc_feature    5980..6652 
                     /label="ColE1"  
     misc_feature    7082..7876 
                     /label="Kan/Neo"  
     misc_feature    7911..8213 
                     /label="SV40 ori"  
     misc_feature    8299..8776 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4980..4986 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4980 
                     /label="Spag9"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCTGGAG GACGGTGTGG TATACCAGGA GGAGCCCGGA GGCTCCGGGG 
     1081 CCGTGATGTC AGAGCGGGTG TCCGGCCTGG CCGGCTCTAT CTACCGCGAG TTCGAGCGGC 
     1141 TCATCGGGCG CTACGACGAG GAGGTGGTCA AGGAGCTGAT GCCACTGGTG GTGGCCGTGC 
     1201 TGGAGAACCT GGACTCGGTG TTCGCGCAGG ACCAGGAGCA CCAGGTGGAG CTGGAGCTGT 
     1261 TGCGGGACGA CAACGAGCAG CTCATCACCC AGTACGAGCG GGAGAAGGCG CTGCGCAAGC 
     1321 ACGCCGAGGA GAAATTCATT GAATTTGAAG ACTCTCAAGA ACAGGAGAAA AAGGATTTGC 
     1381 AGACACGAGT GGAGTCTCTA GAATCTCAAA CAAGACAACT CGAACTCAAA GCAAAGAACT 
     1441 ATGCTGACCA GATCAGCAGA CTTGAAGAGA GAGAAGCAGA ACTGAAGAAG GAGTATAATG 
     1501 CACTGCACCA GAGACACACG GAGATGATCC ATAATTACAT GGAACACTTG GAAAGAACCA 
     1561 AACTTCATCA GCTCTCAGGG AGTGAGCAGC TAGAGGCCAC AGCTCACAGT AGAATTAGAA 
     1621 AAGAGCGTCC TATCTCATTA GGGATTTTCC CTCTACCTGC TGGAGACGGG TTGCTCACAC 
     1681 CTGACACTCA GAAGGGAGGC GAGACCCCAG GATCAGAGCA ATGGAAATTT CAAGAATTAA 
     1741 GTCAACCACG GTCTCATACC AGCCTGAAGG ATGAACTTTC TGATATTAGT CAGGGTGGAT 
     1801 CTAAAGCTAC CACTCCAGCT TCAACAGCAA ATTCGGATGT GTCAGCAATT CCTCCTGATA 
     1861 CTCCGTCAAA GGAAGATAAT GAAGGACTTG TAAAAGGCAC AGATACACCA AATAAGGCAG 
     1921 AGATAAGCAA ACACATAGAA GTACAAGTTG CTCAAGAGAC TAGGAATGTG TCTACAGATT 
     1981 CTGCCGAAAA TGAAGAGAAA TCAGAAGTTC AGGCAATCAT TGAATCCACT CCCGAACTGG 
     2041 ATATGGACAA AGACCTCAGT GGATATAAAG GTTCAAGCAC ACCCACCAAA GGCATAGAGA 
     2101 ACAAAGCTTT CGATCGAAAT ACAGAGTCTC TCTTTGAAGA ACTGTCTTCA GCTGGCTCAG 
     2161 GCCTTATTGG AGATGTGGAT GAAGGAGCAG ATTTACTAGG AATGGGCCGT GAAGTGGAGA 
     2221 ATCTCATACT AGAGAACACG CAGCTGCTGG AAACCAAAAA TGCTTTGAAT GTAGTGAAGA 
     2281 ATGACTTAAT AGCAAAAGTG GATGAGCTGA CCTGTGAGAA GGATGTGCTT CAGGGAGAGC 
     2341 TGGAAGCTGT GAAACAAGCC AAGCTGAAGC TAGAGGAAAA GAACAGAGAA CTGGAGGAGG 
     2401 AGCTCAGGAA AGCTCGTGCA GAAGCTGAAG ATGCAAGGCA AAAAGCAAAA GATGATGATG 
     2461 ATAGCGACAT TCCTACCGCC CAGAGGAAGC GGTTTACCAG AGTGGAAATG GCCAGAGTTC 
     2521 TCATGGAGAG AAACCAGTAC AAAGAGAGGC TGATGGAGCT TCAAGAAGCT GTGCGATGGA 
     2581 CAGAGATGAT CCGGGCCTCC CGAGAAAATC CAGCTATGCA AGAAAAAAAA AGATCAAGCA 
     2641 TCTGGCAATT TTTCAGCCGA CTTTTTAGCT CTTCAAGTAA TGCAGCAAAG AAGCCCGAAC 
     2701 CCCCTGTTAA TCTGAAGTAC AATGCCCCCA CCTCTCACGT GACTCCCTCC GTGAAGAAAA 
     2761 GGAGCAGCAC TTTATCTCAG CTCCCTGGTG ACAAGTCCAA GGCGTTCGAT TTCCTTAGTG 
     2821 AGGAAACTGA GGCTAGCTTA GCCTCCCGCA GAGAGCAAAA GAGAGAGCAG TATCGGCAGG 
     2881 TGAAGGCTCA TGTTCAGAAG GAAGATGGCC GAGTGCAAGC TTTCGGTTGG AGTCTGCCTC 
     2941 AGAAGTACAA GCAGGTAGCC AATGGTCAAG GGGAAAATAA GATGAAAAAT TTACCTGTGC 
     3001 CTGTCTATCT CAGACCACTA GACGAAAAAG ATGCGTCTAT GAAGCTGTGG TGTGCTGTAG 
     3061 GAGTCAACTT ATCCGGCGGC AAGACAAGAG ACGGCGGGTC TGTGGTTGGA GCAAGTGTGT 
     3121 TTTACAAGGA CGTTTCTGCT CTGGAATCTG AAGGCAGTAA ACAGCGGAGT GCGTCTCAGA 
     3181 GTAGTTTAGA TAAGTTGGAT CAAGAACTCA AGGAACAGCA GAAAGAATTA AAAAATCAAG 
     3241 AAGAATTGTC CAGTCAAGTC TGGATCTGTA CCAGCACCCA TTCAACTACA AAGGTTATCA 
     3301 TCATTGATGC TGTTCAACCT GGCAACATCC TAGATAGTTT CACTGTTTGC AATTCTCATG 
     3361 TTCTGTGCAT TGCCAGTGTC CCAGGGGCTC GAGAGACAGA CTACCCTGCA GGAGAAGAGC 
     3421 TTTCTGACTC TGGTCAGGTA GACAGAGCGT CTCTGTGTGG GAGCATGACC AGTAACAGCT 
     3481 CTGCAGAGAT GGACAGCTTG CTGGGAGGCA TCACAGTGGT CGGGTGCTCC ACAGAAGGAG 
     3541 TGACGGGAGC TGCTGCTTCC CCAAGTACCA ATGGTGCTTC TCCGGTGATT GAAAAGCCAC 
     3601 CAGAGGTGGA GGCTGAAAAC AGTGAGATTG ATGAAAACAT TCCAACAGCA GAGGAAGCCA 
     3661 CTGAAGCCAC AGAGGGCAAT GCAGGATCCA CTGAAGACGC TGTGGACATC TCCCAGCCTG 
     3721 GCGTGTACAC AGAACATGTG TTTACAGATC CTCTGGGAGT TCAGATCCCA GAAGACCTCT 
     3781 CCCCAGTGTT TCAGTCAAGT AATGACTCAG ATGTGTACAA AGATCAAATA TCAGTATTGC 
     3841 CAAATGAACA AGACCTTGCG AGAGAAGAAG CTCAGAAAAT GAGTAGTCTT TTACCAACCA 
     3901 TGTGGCTTGG AGCTCAAAAT GGCTGTTTGT ATGTCCACTC GTCTGTAGCC CAGTGGAGGA 
     3961 AATGTCTCCA TTCCATTAAG CTCAAAGACT CAATCCTCAG TATTGTACAC GTGAAAGGAA 
     4021 TTGTGCTGGT GGCCCTGGCT GATGGCACCC TTGCAATCTT CCACAGAGGA CTTGATGGAC 
     4081 AGTGGGACCT GTCAAACTAT CACCTCTTAG ACCTCGGACG CCCTCATCAC TCCATAAGAT 
     4141 GCATGACCGT GGTGCATGAC AAAGTCTGGT GTGGCTATAG GAACAAAATC TATGTGGTTC 
     4201 AGCCAAAGGC TATGAAGATA GAGAAATCAT TTGATGCACA CCCCAGGAAG GAGAGCCAAG 
     4261 TACGGCAGCT TGCGTGGGTG GGTGATGGCG TATGGGTTTC CATCCGCTTG GATTCCACGC 
     4321 TCCGCCTCTA TCATGCACAT ACATACCAAC ATCTCCAGGA TGTGGACATT GAACCTTATG 
     4381 TAAGCAAAAT GTTAGGTACT GGAAAACTGG GTTTCTCTTT TGTGAGGATA ACAGCTCTTA 
     4441 TGGTGTCTTG CAATCGTTTG TGGGTGGGGA CTGGAAACGG TGTCATTATC TCCATCCCAT 
     4501 TGACAGAAAC TGTAATCCTC CACCAGGGAC GTTTACTGGG GCTGAGGGCA AATAAAACCT 
     4561 CAGGGACTCC AGGTAATCGT CCTGGAAGTG TAATCCGTGT ATATGGTGAT GAAAACAGTG 
     4621 ACAAAGTGAC TCCAGGGACA TTTATACCCT ACTGTTCGAT GGCACATGCG CAGCTTTGTT 
     4681 TCCATGGGCA CCGGGATGCT GTGAAATTCT TTGTGGCAGT CCCCGGTCAG GTCGTTAGCC 
     4741 CACAGAGTAG CAGTAGTGGA GCAGATCTAA CAGCTGACAA AGCAGGCTCG TCTGCACAGG 
     4801 AGCCCAGTAG CCAGACGCCC TTGAAGTCTA TGCTTGTCAT CAGTGGAGGA GAAGGCTACA 
     4861 TTGACTTCCG AATGGGTGAT GAAGGTGGAG AATCTGAACT TCTTGGAGAG GATCTTCCAC 
     4921 TGGAACCTTC AGTCACCAAA GCAGAAAGGA GTCACCTGAT AGTGTGGCAG GTGATGTGTG 
     4981 GCAATGAAAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     5041 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     5101 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     5161 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     5221 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     5281 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     5341 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     5401 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     5461 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5521 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5581 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5641 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5701 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5761 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5821 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5881 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5941 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     6001 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     6061 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     6121 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     6181 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     6241 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     6301 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     6361 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     6421 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     6481 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6541 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6601 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6661 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6721 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6781 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6841 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6901 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6961 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     7021 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     7081 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     7141 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     7201 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     7261 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     7321 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     7381 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     7441 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     7501 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7561 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7621 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7681 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7741 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7801 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7861 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7921 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7981 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     8041 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     8101 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8161 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8221 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     8281 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     8341 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     8401 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     8461 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     8521 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     8581 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8641 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8701 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8761 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8821 CAAAAATTTA ACGCGAATTT T
//