LOCUS       RR209418                8460 bp    DNA     circular  SYN 
DEFINITION  Prtg (Myc-DDK-tagged ORF) - Rat protogenin homolog (Gallus gallus) (Prtg), (10 ug)
ACCESSION   NM_001037651
VERSION     NM_001037651.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4617..4708 
                     /label="Myc-DDK"  
     misc_feature    4738..5323 
                     /label="PolyA signal"  
     misc_feature    4795..4814 
                     /label="Restriction Enzyme"  
     misc_feature    5599..6271 
                     /label="ColE1"  
     misc_feature    6701..7495 
                     /label="Kan/Neo"  
     misc_feature    7530..7832 
                     /label="SV40 ori"  
     misc_feature    7918..8395 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4599..4605 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4599 
                     /label="Prtg"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGCCTCCC GTGCGCCCGG GGATGCTGCC CCTGCTGCTG TTGCTGCTAC 
     1081 TGCCGCCGCT CGGCTCGGTT CCAGGAGTGT GGAGCTTTAG CGAACTGTTC TTCATGAAAG 
     1141 AACCACAGGA CGCAACGGTC ACAAGGAAAG ACCCAGTCTT CTTAGATTGC CAGGCCCATG 
     1201 GAGAAGGTCC CATTAAGGTC ACCTGGTTGA AAAATGGAGC AAAATTGTCT GAAAATAAAC 
     1261 GGATCCAGGT TCTATCTAAC GGCTCTTTAT ACATCAGTGA GGCGGAAGGC AGGCGGGGAG 
     1321 AGCAATCGGA TGAAGGATTT TATCAGTGCT TGGCTGTAAA CAAATATGGG GCCATTCTTA 
     1381 GTCAGAAAGC TCATCTGACA TTGTCAACCA TCTCTGCGTT CGAGGTCCAT CCAGTGTCTA 
     1441 CCGAAGTCCC CGAAGGGGGA GTTGCTAGAT TTTCATGCAA GATTTCATCA ACGCCCCCTG 
     1501 CGGTCATAAC ATGGGAATTT AATAGGACAG CCCTGCCTAT GACCATGGAC AGCAGGGTGA 
     1561 CTGCCCTGCC CTCAGGAGTG CTGCAGATCT ATGACGCTGG CCCGGAGGAT GCTGGGAAAT 
     1621 ACCGCTGTGT TGCAGCCACG CATGCTCATA AGCGTAAAAG CATGGAGGCC TCGCTAACCA 
     1681 TAGTTCCAGC TAATGAGACC AGATCCTTCT ACATGCCCAC CATTATAGCC AGTCCACAGA 
     1741 ATGTAACCGC GTCTCTACAT CAGACAGTTG TGCTAGAGTG TATGGCTACA GGATATCCCA 
     1801 AGCCCATCAT TTCCTGGAGC CGTCTTGATC ACAAGTCCAT TGATGTCTTC AATACTCGGG 
     1861 TACTCGGAAA TGGCAATCTC ATCATATCAG ATGTCAAGCT TCAGCATGCT GGAGTGTATG 
     1921 TTTGTCGGGC CACCACCCCA GGCACTCGCA ACTTCACAGT TGCTATGGCA ACGTTAACTG 
     1981 TATTAGCTCC TCCTTCATTT GTGGAATGGC CTGAAAGTTT AACAAGGCCA CGAGCTGGCA 
     2041 CTGCACGGTT TGTGTGTCAA GCAGAAGGAA TTCCCTCACC CAAAATGTCC TGGTTGAAAA 
     2101 ATGGGAGAAG GATACATTCA AATGGCAGGA TTAAAATGTA CAACAGCAAA TTGGTCATTA 
     2161 ACCAGATTAT CCCAGAAGAT GATGCTATCT ATCAGTGCAT GGCTGAAAAC AGCCAAGGAT 
     2221 CTGTTCTGTC TCGAGCCAGA CTGACTGTGG TGATGTCGGA AGATAGACCC AGCGCTCCTT 
     2281 ACAACGTTCA TGCCGAAACC ATGTCGAGCT CTGCTATCCT TCTAGCATGG GAGCGACCAC 
     2341 TGTACAATTC AGACAAGGTC ATTGCCTACT CTGTACACTA CATGAAGGCT GAAGGTCTAA 
     2401 ATAACGAGGA ATATCAGGTG GTCCTTGGAA ATGACACAAC TCATTATATC ATCGATGACT 
     2461 TGGAACCAGA CAGCAACTAT ACTTTCTACA TCGTGGCGTA CATGCCCATG GGAGCCAGCC 
     2521 AGATGTCGGA TCATGTGACA CAGAACACGC TAGAAGACGT GCCCCTAAGG CCTCCCGAAA 
     2581 TTAGCTTGAC GAGTCGAAGT CCCACCGACA TTCTCATCTC CTGGCTGCCC ATCCCGGCCA 
     2641 AGTACCGACG GGGCCAAGTG GTGCTGTACC GGCTGTCCTT CCGCCTGAGC ACTGAGAATT 
     2701 CCATCCAAGT TGTAGAACTC CCGGGGACTG TGCACGAGTA CCTTCTGGAA GGCCTGGAGC 
     2761 CAGACAGTGT CTATCTGGTC CGGATTACTG CTGCTACCCG AGTGGGGCTG GGAGAGTCAT 
     2821 CGGTGTGGAC GTCACACAGG ACGCCCAAAG CCACGAGCGT GAAAGCCCCT AAGTCTCCTG 
     2881 AACTGCATTT GGAGCCACTG AATTGTACTA CCATCTCTGT AAGGTGGCTT CAAGACACCG 
     2941 AGGACCCGGC TGCCATTCGG GGCTACAAGC TGTTTTATAA AGAAGAAGGG CAGCAGGAAC 
     3001 ATGGACCGAT CTTCCTGGAC ACCGGTGACC TGCTGTATAC GCTCAGTGGC TTGGACCCCA 
     3061 GAAGAAAATA CCATGTGAGG CTACTGGCTT ACAACAATCT GGAAGATGGC TACCAGGCCG 
     3121 ACCAGACCGT CAGTACTCCC GGATGCGTGT CTGTCCGTGA CCGCATGGTC CCTCCGCCAC 
     3181 CGCCACCCCA CCATCTGTAC GCGAAGGCTA ATACCTCCTC TTCTATCTTC CTGCACTGGA 
     3241 GGAGGCCTGC GTTCACCACC GCCCAAGTCA TTAACTACAC CATCCGCTGT AACCCCGTTG 
     3301 GCCTGCAGAA CGCCTCTCTG GTTCTCTACC TGCAAACATC AGAAACCCAC ATGCTGGTTC 
     3361 AAGGACTAGA GCCAAACACC AAGTATGAGT TCGCTGTCCG ACTGCACGTG GATCAGCTCT 
     3421 CCAGTCCCTG GAGTCCCGTG GTCTACCACA CCACACTGCC GGAAGCACCC ACAGGCCCCC 
     3481 CAGTGGGGGT GAAGGTGACG CTGATAGAAG ATGACACTGC CCTTGTCTCT TGGAAACCCC 
     3541 CAGATGGCCC AGAGACAGTG GTGACGCGCT ACACCATCTT GTATGCGTCC AGGAAGGCCT 
     3601 GGATCGCCGG CGAGTGGCAG GTCCTACATC GAGAAGGGGC AATAACCATG GCTCTGCTGG 
     3661 AAAACCTGGT GGCAGGAAAC GTGTACATTG TCAAGATCTC AGCCTCCAAC GAGGTGGGAG 
     3721 AAGGGCCCTT CCCAAATTCC GTGGAGCTGG CCGTCCTCCC CAAGGACGCT TCAGAATCGA 
     3781 ACCAGAGGCC TAAGCGTTTG GATTCTTCTG ATGCCAAAGT TTATTCAGGC TATTACCACC 
     3841 TGGACCAAAA GTCAATGACC GGCATTGCAG TAGGCGTTGG CATAGCCTTG ACCTGTATCC 
     3901 TCATCTGTGT TCTCATCTTG ATATACCGAA GCAAAGCCAG GAAGTCATCC GCCTCTAAGA 
     3961 CAACGCAGAG TGGAACCCAG CCGTTATCCC GAGCCAGTGC CTCTGTAGCA GCGGGGAGTG 
     4021 ACATGGGGAA GAACCTGGAG AGAGCTACAG AAAACGAAGA GTCCTCAGTA CCCATGATGC 
     4081 CAAGCTGCTT CATTGATGCG AAGGGAGGGA CTGACCTGAT CATCAATAGC TATGGTCCTA 
     4141 TTATTAAAAA CAACCCTAAG AAAAAGTGGC GTTTTTTCCA AGACACTAAG AAGATCAAAG 
     4201 TTGAACAGAC TCAAAGAAGA ATTACTCAGA CCGTGTGCTT TTACCAGCCA GGCACCACAG 
     4261 TGCTGATCAG CGATGAAGAC TCCCCTAGCT CCCCAGGCCA GACCACCAGC TTCCCAAGAC 
     4321 CTTTTGGGCC CACCACCCTG GACACTGAGC ACTCAGCAAA CAGTGAAGGC AGCCACGAGA 
     4381 CAGGGGATTC TGGAAGGTTT TCCCATGAGT CCAATGATGA GATACATCTG TCCTCTGTCA 
     4441 TCAGCAGCAC ACCCCCCACC TCGAATTCCC TCACCTGTGG TGATTCTGAT GGGGATGCTG 
     4501 CACCGAAGAA GCATGGAGAC CCTGCCCAGC CCTTGCCAGC TGAGCAGACC TCAGCCCCAC 
     4561 AGCCAACACC TGCTGGGCTG CGACATGCTG CCGAGAGTGT TCCCGTCACG CGTACGCGGC 
     4621 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4681 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4741 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4801 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4861 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4921 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4981 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     5041 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     5101 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     5161 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     5221 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     5281 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5341 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     5401 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     5461 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     5521 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     5581 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5641 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5701 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5761 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     5821 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     5881 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     5941 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     6001 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     6061 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     6121 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     6181 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     6241 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     6301 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6361 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6421 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6481 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6541 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6601 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6661 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6721 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6781 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6841 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     6901 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     6961 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     7021 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     7081 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     7141 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     7201 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     7261 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     7321 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7381 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7441 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7501 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7561 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7621 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7681 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7741 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7801 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7861 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     7921 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     7981 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     8041 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     8101 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     8161 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     8221 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     8281 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     8341 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     8401 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//