LOCUS       RR209404                6717 bp    DNA     circular  SYN 
DEFINITION  Cel (Myc-DDK-tagged ORF) - Rat carboxyl ester lipase (Cel), (10 ug)
ACCESSION   NM_016997
VERSION     NM_016997.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2874..2965 
                     /label="Myc-DDK"  
     misc_feature    2995..3580 
                     /label="PolyA signal"  
     misc_feature    3052..3071 
                     /label="Restriction Enzyme"  
     misc_feature    3856..4528 
                     /label="ColE1"  
     misc_feature    4958..5752 
                     /label="Kan/Neo"  
     misc_feature    5787..6089 
                     /label="SV40 ori"  
     misc_feature    6175..6652 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2856..2862 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2856 
                     /label="Cel"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGCGCCTG GAGGTTCTGT TTCTTGGCCT CACCTGCTGC TTGGCAGCTG 
     1081 CTTGTGCTGC AAAGTTGGGT GCTGTGTACA CAGAAGGCGG TTTTGTGGAG GGCGTCAACA 
     1141 AGAAACTCAG TCTCTTGGGT GGTGACTCTG TTGACATCTT CAAGGGCATC CCCTTTGCTA 
     1201 CCGCCAAGAC CCTGGAGAAT CCTCAGCGTC ACCCTGGCTG GCAAGGGACA CTGAAGGCTA 
     1261 CAGACTTCAA GAAACGATGT CTACAAGCCA CCATCACCCA GGATGATACC TATGGGCAAG 
     1321 AAGACTGCCT CTATCTCAAC ATCTGGGTCC CTCAGGGCAG GAAGCAAGTG TCTCATGACC 
     1381 TGCCTGTGAT GGTCTGGATC TATGGAGGTG CCTTCCTCAT GGGGTCTGGC CAGGGAGCCA 
     1441 ATTTTCTCAA GAATTACCTG TATGATGGGG AAGAGATCGC CACTAGAGGC AATGTCATTG 
     1501 TGGTCACCTT CAACTACCGT GTCGGACCCT TGGGTTTCCT TAGCACCGGA GATGCTAACC 
     1561 TTCCAGGTAA CTTTGGACTT CGAGATCAGC ACATGGCTAT TGCCTGGGTG AAGAGGAACA 
     1621 TTGCAGCCTT TGGAGGAGAC CCCGATAACA TCACCATCTT TGGGGAATCT GCTGGAGCTG 
     1681 CCAGTGTCTC TCTGCAGACC CTCTCCCCAT ACAACAAGGG CCTCATCCGG CGAGCCATCA 
     1741 GTCAGAGTGG TGTGGCACTG AGCCCCTGGG CCATCCAGGA GAATCCACTT TTCTGGGCCA 
     1801 AAACGATCGC TAAGAAGGTG GGATGCCCCA CAGAGGATAC CGCCAAGATG GCTGGGTGTC 
     1861 TGAAGATCAC AGATCCCCGA GCCTTGACAC TGGCCTATAG GTTGCCCTTG AAAAGCCAGG 
     1921 AGTACCCCAT TGTGCACTAC CTGGCCTTCA TCCCTGTCGT CGATGGTGAC TTCATTCCTG 
     1981 ATGATCCCAT CAACCTGTAC GACAACGCTG CTGACATTGA CTACTTAGCG GGTATTAATG 
     2041 ACATGGATGG CCACCTGTTT GCTACAGTTG ACGTGCCCGC CATCGACAAG GCCAAGCAGG 
     2101 ATGTCACAGA GGAGGACTTC TACAGGCTAG TCAGTGGACA CACTGTCGCC AAGGGGCTTA 
     2161 AAGGCACCCA AGCCACCTTT GACATCTACA CTGAGTCCTG GGCCCAGGAC CCGTCCCAGG 
     2221 AGAACATGAA GAAGACAGTG GTGGCCTTTG AGACTGACAT ACTCTTCCTG ATCCCCACAG 
     2281 AGATGGCTCT GGCCCAGCAC AGAGCCCATG CCAAGAGTGC CAAGACCTAC TCTTACCTGT 
     2341 TTTCCCACCC TTCACGAATG CCTATCTACC CAAAATGGAT GGGGGCAGAC CACGCTGATG 
     2401 ACCTCCAGTA CGTCTTTGGG AAGCCCTTTG CCACCCCACT GGGCTACCGG GCCCAAGACA 
     2461 GGACTGTCTC CAAGGCCATG ATTGCCTACT GGACCAACTT TGCCAAGAGT GGGGACCCCA 
     2521 ACATGGGCAA CTCACCCGTG CCCACACACT GGTACCCTTA TACCACGGAG AATGGTAACT 
     2581 ACCTGGACAT CAATAAGAAA ATAACCAGCA CCTCCATGAA GGAGCACCTA AGGGAAAAGT 
     2641 TCCTCAAGTT CTGGGCTGTG ACATTCGAGA TGCTGCCCAC TGTGGTTGGT GACCACACTC 
     2701 CCCCTGAGGA TGACTCAGAG GCTGCCCCCG TCCCACCTAC AGACGACTCC CAGGTTGTTC 
     2761 CTGTCCCACC TACAGATGAC TCTCAGACAA CACCGGTGCC CCCAACAGAC AACTCTCAGG 
     2821 CTGGTGACTC TGTGGAGGCT CAGATGCCTG TCGCCATTGG CTTCACGCGT ACGCGGCCGC 
     2881 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     2941 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     3001 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     3061 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     3121 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     3181 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     3241 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     3301 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     3361 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     3421 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     3481 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     3541 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     3601 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     3661 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     3721 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     3781 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     3841 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     3901 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     3961 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     4021 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     4081 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     4141 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     4201 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     4261 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     4321 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     4381 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     4441 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     4501 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     4561 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     4621 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     4681 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     4741 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     4801 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     4861 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     4921 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     4981 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     5041 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     5101 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     5161 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     5221 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     5281 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     5341 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     5401 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     5461 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     5521 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     5581 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     5641 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     5701 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     5761 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     5821 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     5881 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     5941 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     6001 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     6061 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     6121 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     6181 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     6241 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     6301 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     6361 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     6421 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     6481 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     6541 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     6601 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     6661 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//