LOCUS       RR209390                8502 bp    DNA     circular  SYN 
DEFINITION  Ap3d1 (Myc-DDK-tagged ORF) - Rat adaptor-related protein complex 3, delta 1 subunit (Ap3d1), (10 ug)
ACCESSION   NM_001100719
VERSION     NM_001100719.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4657..4748 
                     /label="Myc-DDK"  
     misc_feature    4778..5363 
                     /label="PolyA signal"  
     misc_feature    4835..4854 
                     /label="Restriction Enzyme"  
     misc_feature    5639..6311 
                     /label="ColE1"  
     misc_feature    6741..7535 
                     /label="Kan/Neo"  
     misc_feature    7570..7872 
                     /label="SV40 ori"  
     misc_feature    7958..8435 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4632..4639 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4632 
                     /label="Ap3d1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCCTCAAG ATGGTCAAGG GCAGTATCGA CCGCATGTTC GACAAGAACC 
     1081 TGCAGGATCT GGTCCGCGGC ATCCGCAACC ACAAGGAGGA CGAGGCCAAG TACATCTCCC 
     1141 AGTGCATTGA CGAGATCAAG CAGGAACTCA AGCAGGACAA CATTGCTGTG AAGGCCAACG 
     1201 CTGTCTGCAA GCTCACCTAC TTACAGATGC TCGGGTACGA CATCAGCTGG GCTGCCTTCA 
     1261 ACATCATTGA AGTCATGAGT GCCTCCAAGT TCACATTCAA GCGTGTCGGC TACCTTGCAG 
     1321 CCTCTCAGTG CTTCCATGAA GGTACTGACG TCATCATGCT GACCACTAAC CAGATCCGAA 
     1381 AGGACCTGAG CAGCCCTAGC CAGTACGACA CTGGAGTGGC ACTGACTGGT CTGTCCTGTT 
     1441 TTGTCACCCC AGATCTTGCC AGAGACCTGG CAAATGACAT CATGACCCTG ATGTCTCACA 
     1501 CGAAGCCATA CATCAGGAAG AAGGCAGTGC TGATCATGTA CAAAGTGTTC CTCAAGTACC 
     1561 CTGAGTCACT GCGTCCTGCC TTTCCCCGGC TTAAGGAGAA GCTGGAGGAC CCAGACCCAG 
     1621 GGGTGCAGTC TGCAGCTGTC AATGTCATCT GTGAGCTAGC GCGCCGAAAC CCTAAAAACT 
     1681 ACCTGTCTCT GGCTCCACTG TTTTTCAAGC TCATGACATC CTCTACCAAC AACTGGGTCC 
     1741 TCATCAAGAT CATCAAACTG TTCGGTGCCC TGACTCCCCT GGAGCCCAGG CTGGGCAAGA 
     1801 AGCTGATCGA GCCTCTCACC AACCTCATTC ACAGCACCTC TGCCATGTCC CTGCTGTACG 
     1861 AGTGTGTGAA CACCGTGATT GCTGTGCTCA TCTCACTGTC TTCCGGGATG CCCAACCACA 
     1921 GCGCCAGCAT CCAGCTATGC GTTCAGAAGT TGAGGATACT GATAGAGGAC TCCGACCAGA 
     1981 ACTTAAAGTA CCTGGGGCTG CTGGCCATGT CTAAAATCCT GAAGACACAT CCCAAGTCTG 
     2041 TGCAATCCCA CAAGGACCTG ATCCTGCAGT GCCTGGATGA CAAAGACGAG TCCATCCGCC 
     2101 TGCGTGCCCT TGACCTGCTC TATGGAATGG TGTCCAAAAA GAACCTGATG GAAATTGTGA 
     2161 AGAAGCTTAT GACCCACGTG GACAAGGCCG AGGGCACCAC CTATCGTGAC GAGCTGCTCA 
     2221 CCAAGATCAT TGACATCTGT AGCCAGTCCA ACTACCAGCA CATCACCAAC TTCGAGTGGT 
     2281 ACATAAGCAT CCTTGTGGAG CTGACAAGAC TGGAGGGCAC CCGGCATGGT CACCTCATTG 
     2341 CTGCACAGAT GCTGGACGTG GCCATTCGGG TCAAGGCCAT TCGAAAATTT GCTGTGTCCC 
     2401 AGATGTCTTC GCTGCTTGAT AGTGCCCACC TGGTAGCCAG CAGCACCCAA CGCAACGGCA 
     2461 TCTGTGAGGT GCTCTATGCT GCTGCCTGGA TCTGCGGGGA GTTCTCGGAG CACCTACAGG 
     2521 GGCCCCAGCA AACCCTGGAG GCCATGCTGC GGCCCAAGGT CACCACACTG CCCGGCCACA 
     2581 TCCAGGCTGT GTATGTGCAG AACGTGGTAA AGCTGTACGC GTCCATCCTG CAGCAGAAGG 
     2641 AGCAGGCTGC CGACACTGAG GGGGCACAGG AGGTCACCCA GCTACTAGTG GAGCGGCTGC 
     2701 CACAGTTTGT GCAGAGCGCC GACCTGGAGG TGCAGGAGCG GGCGTCTTGC ATCCTGCAGC 
     2761 TGGTCAAGCA TGTACAGAAG CTGCAAGCAA AAGGCGTGCC GGTGGCCGAG GAAGTGAGCG 
     2821 CCTTGTTTGC TGGGGAACTG AACCCTGTGG CCCCCAAGGC CCAGAAGAAG GTCCCAGTTC 
     2881 CTGAAGGCCT AGACCTGGAT GCCTGGATCA ATGAGCCACC TTCAGACAGT GAGTCTGAGG 
     2941 ATGAGAAACC CAAGGCCATC TTTCATGAAG AGGAGCCAAG GCACACCCGG CGCCGGCAGC 
     3001 CTGAGGAGGA TGAGGAGGAG CTGGCCCGGC GGCGAGAGGC CCGGAAGCAG GAGCAAGCCA 
     3061 ACAATCCCTT CTACATCAAG AGCTCCCCAT CCCCGCAGAA GCGATACCAA GACGCACCAG 
     3121 GCGTGGAGCA CATTCCTGTG GTACAGATCG ACCTCTCCGT GCCTCTGAAA GTTCCAGGTA 
     3181 TGCCCATGTC AGACCAGTAT GTGAAGCTGG AGGAACAGCG GCGACACCGG CAGCGGCTGG 
     3241 AGAAGGACAA GAAAAGGAAG AAGAAGGAGA AGGGCAAGCG TCGCCACAGT TCCCTGCCCA 
     3301 CGGAGAGTGA TGAGGACATC GCCCCCGCCC AGCGTGTGGA CATCATCACT GAGGAGATGC 
     3361 CTGAGAATGC TCTGCCTAGT GATGAGGATG ACAAAGACCC CAATGACCCC TACAGGGCAC 
     3421 TGGACATTGA CCTAGATAAG CCCTTGGCCG ACAGTGAGAA GCTGCCTGTC CAGAAACATA 
     3481 GAAACGCGGA GACAGTGAAG TCCCCAGAGA AGGAGGGTGT GCCTGGGGTG GAGAAGAAGA 
     3541 GCAAGAAGCC CAAGAAGAAG GAGAAGAAGC CCAAAGAGAG AGAAAGGGAG AAGAAGGACA 
     3601 AGAAGGGTGA GGACTTAGAC TTCTGGCTGT CCACCACCCC GCCACCTGCT GCTGCCCCCG 
     3661 TCCCTGCCCC ATCTACGGAA GGACTTGCTG CGAGCATTGT CACCCCCCCT AAGGGTGAGT 
     3721 GTGAGGTTCT CAAAGGGGAA GAGCCCCAGG ACGAGGAGGA CCACATGGAT CATGACCAAG 
     3781 AAAGGAAATC ATCCCGGCAC AAGAAGAAGA AACACAGGAA GGAGAAGGAG GAGAGGCCAC 
     3841 GAGACAAAAA GAAAGCCAAG AGGAAGCAGG TGGTGCAGCT AGAGAATGGT GCGCCAGCAG 
     3901 AGGAGGAAGA GGAGGAGGAG GAACCTATCC CGCCCATGTC CAGTTACTGC CTTCTGGCTG 
     3961 AGAATCCGTA CATTAAAGTG ACATATGACG TCCAAGCCAG CTTACAGAAG GACAGCCAAG 
     4021 TGACTGTGTC TATCATCCTG GAGAATCAGA GCAGCAGCTT CCTGAAGAAC ATGGAACTCA 
     4081 ACGTGCTAGA CTCACTCAAC ACCAAGATGG CCAGGCCAGA GGGCTCATCA GTGCATGATG 
     4141 GTGTGCCTGT GCCTTTCCAG CTGCCCCCCG GCGTCTCCAA TGAGGCCCAG TTTGTGTTCA 
     4201 CCATTCAGAG CATCGTCATG GCCCAGAAGC TCAAAGGCAC GCTGTCCTTC ATTGCCAAGG 
     4261 ATGATGAAGG AGCCACCCAT GAGAAGTTGG ACTTCCGGCT GCATTTCAGC TGTAGCTCGT 
     4321 ACCTGATCAC CACCCCCTGC TACAGCGATG CCTTTGCCAA GTTGCTGGAG TCAGGAGACC 
     4381 TGAGCATGAA CTCCATCAAA GTTGATGGCA TTAGTATATC TTTCCAGAAT CTTCTGGCAA 
     4441 AGATCTGCTT CTACCACCAT TTTTCCGTTG TGGAGCGAGT AGACTCCTGC GCCTCCATGT 
     4501 ACAGCCGCTC TATCCAGGGC CATCACGTCT GCCTGCTGGT GAAGAAGGGT GAGAGCTCCG 
     4561 TGTCAGTGGA CGGGAAGTGC AGCGATGCCA CGCTGCTCAG CAGCCTGCTG GAGGAAATGA 
     4621 AGACCACGCT GTCTCAGTGC AGCGGACCGA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     4681 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     4741 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4801 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4861 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4921 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4981 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     5041 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     5101 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     5161 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     5221 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     5281 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     5341 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     5401 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     5461 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     5521 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     5581 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     5641 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     5701 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     5761 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5821 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     5881 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     5941 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     6001 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     6061 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     6121 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     6181 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     6241 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     6301 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     6361 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     6421 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     6481 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6541 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6601 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     6661 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     6721 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6781 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6841 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6901 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     6961 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     7021 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     7081 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     7141 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     7201 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     7261 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     7321 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     7381 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     7441 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     7501 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7561 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7621 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     7681 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     7741 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7801 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7861 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7921 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     7981 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     8041 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     8101 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     8161 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     8221 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     8281 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     8341 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     8401 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     8461 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//