LOCUS       RR209370                7662 bp    DNA     circular  SYN 
DEFINITION  Ern2 (Myc-DDK-tagged ORF) - Rat endoplasmic reticulum to nucleus signaling 2 (Ern2), (10 ug)
ACCESSION   NM_001108919
VERSION     NM_001108919.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3819..3910 
                     /label="Myc-DDK"  
     misc_feature    3940..4525 
                     /label="PolyA signal"  
     misc_feature    3997..4016 
                     /label="Restriction Enzyme"  
     misc_feature    4801..5473 
                     /label="ColE1"  
     misc_feature    5903..6697 
                     /label="Kan/Neo"  
     misc_feature    6732..7034 
                     /label="SV40 ori"  
     misc_feature    7120..7597 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3801..3807 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3801 
                     /label="Ern2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGAGACCA GTCCAGAGTT TCCAACTGTG GTCTGCCCTC GGGTTCCTGC 
     1081 TGCAGCTTGC AATCTTTCTG GGGAAACTCG GTCCACAGGT CCAATCCGTC AGGCCAGAGA 
     1141 GCCTTCTGTT TGTCTCTACC TTGGATGGAA GTCTCCATGC ACTGAACAAG CAGACAGGGG 
     1201 ACTTGAAGTG GACAGTGAAG GATGACCCCA TCATCCAGGG ACCAATGTAT GTCACAGAAA 
     1261 TGGCCTTCCT CTCTGACCCA GCTGATGGCA GCCTGTACAT CTTGGGAACC CAGAAACAAC 
     1321 AGGGGCTGAT GAAACTGCCA TTTACCATCC CAGAGCTGGT TCACGCCTCT CCTTGTCGCA 
     1381 GCTCTGATGG TGTCTTCTAC ACGGGCCGGA AGCAGGATGC CTGGTTTGTG GTGGACCCTG 
     1441 AGTCAGGGGA GACTCAGATG ACACTGACCA CAGAGGGCTT TTCTACTCCT CAACTCTACA 
     1501 TTGGCCGGAC ACAGTACACA GTCTCCATGC ATGACCCACG GACCCCAACC CTGCGCTGGA 
     1561 ACACAACCTA CCGCCGCTAC TCTGCGCCCC CCGTGGACGG CTCACCTGGG AAATACATGA 
     1621 GCCACTTGAC ATCCTGTGGG ATGGGCCTGC TTCTCACTGT GGATCCAGGA AGTGGAACTG 
     1681 TGCTGTGGAC ACAGGACCTG GGAGTGCCTG TGACAGGGAT CTACACATGG CATCAGGATG 
     1741 GTCTGCGACA GCTGCCCCAT CTTACCCTGG CTCGGGACAC CCTACATTTC CTTGTCCTCC 
     1801 GCTGGGGCCA CATCCGTCTT CCTGCCTCCA GCTCCCAGGA CACAGTCACC CAGTTCTCTT 
     1861 CCTTAGACAC CGAACTTTTG ATGACACTGT ATGTGGGGAA AGAAGAAGCT GGTTTCTATG 
     1921 TCTCTAAAGC ACTGGTCCAT GCTGGGGTGG CCCTTGTGCC CCGTGGACTG ACCCTGGCAC 
     1981 CCATGGATGG CCCCACAACA GATGAGGTAA CAGTTCAAGT CTCGGGGGAG CGAGAGGGCT 
     2041 CACCCAGCAC TGCTGTCAGA TACCCCTCGG GCAGCGTGGC CCTTCCCAGC CAGTGGCTAC 
     2101 TCATTGGATA CCATGAACCT CCCCCTGTCC TGCACACCAC CATGCTAAGG GTTCAGCGTA 
     2161 CCCCAGGGAA AGGGTCTGCT GACACAAGAG ACTCAGACGA CCTCCATGCT CCAGCTCTCT 
     2221 CCTTTGAGTT CCTAAACCTG AAGAGGGAGG AACCTTGGGA TCCAGAACTA CATCCTGAAG 
     2281 AGAAAGCTGA CACTTATCCA GGGCTAGGAT CCCAAGACCT GCTAGCAGCT AGCTTCACTG 
     2341 CCATTCTCTT GGGAGCCTGG GTCCTCTACC TGATGAGGCA GCAGCAGCAG AGTCCTTCAG 
     2401 CACCTGCAGG CCCTTCAGAC CTCCCACAAG ACACTCAGGT GCAGCTCTCA AGGGACACCC 
     2461 TACAGAACCA GAGGAGATTT CAAAGCCCCC CAGAGCCAGT CCAGCCAGCC CATGATCCTG 
     2521 AAGCAGAGCA GCCCACTGTA GTGGGGAAGA TTTCCTTCAA CCCCAAGGAT GTGCTGGGCC 
     2581 GTGGGGCAGG AGGAACTTTT GTTTTCCGAG GACAGTTTGA GGGACGGGCA GTAGCTGTGA 
     2641 AGAGGCTCCT CCGAGAGTGC TTCAGCTTGG TTCAGCGAGA GGTGCAGCTG CTGCAAGAGT 
     2701 CTGACAGGCA CCCCAATGTG CTGCGCTACT TCTGCACGGA GCAGGGCCCC CAGTTCCACT 
     2761 ACATTGCCTT GGAACTCTGC CAGGCCTCCT TGCAAGAGTA TGTGGAGAGC CCAGACCTAG 
     2821 ACCGCTGGGG CCTTGGGCCT ACCATGGTGC TGCAACAGAT GATGTCCGGC CTGGCCCACC 
     2881 TGCATTCTTT ACACATAGTA CACCGTGACC TCAAGCCGGG CAACATTCTC ATGGCTGGGC 
     2941 CTGACAGCCA GGGCCAAGGC AGAGTGGTCA TCTCTGACTT CGGCCTCTGC AAGAAGTTAC 
     3001 CTGCTGGCCG TTGTAGCTTC AGTCTCCATT CTGGTATTCC CGGCACAGAA GGCTGGATGG 
     3061 CTCCAGAGCT ACTGCAACTG CCCCCAGACA GCCCGACCAG CGCGGTGGAT ATCTTCTCTG 
     3121 CAGGCTGTGT GTTTTATTAT GTGCTTTCTG GTGGCAGCCA CCCCTTCGGA GAGAGTCTCT 
     3181 ACCGCCAGGC CAACATCCTC TCAGGGGACC ACTGTCTGGC TCAACTGCAG GAAGAGACCC 
     3241 ATGACAAGGT TGTGGCACTG GACTTAGTGA AAGCCATGCT GAGCCTGCTG CCCCAGGATC 
     3301 GTCCCTCAGC AGGATGGGTG CTGGCTCACC CCCTCTTTTG GAGCAGAGCT AAGGAACTCC 
     3361 AGTTCTTCCA GGATGTCAGT GACTGGTTGG AGAAGGAACC AGAGCAGGGA CCTCTCGTGA 
     3421 CGGCGCTGGA GGCAGGAAGC TACAAGGTGG TCCGGGAAAA CTGGTACAAG CACATCTCAG 
     3481 CACCTCTGCA GGAAGACCTG AAAAGGTTCC GGTCATATAA AGGGACATCA GTGCGAGACC 
     3541 TGCTCCGTGC CATGAGGAAC AAGAAGCACC ACTACAGGGA GCTCCCAACC GAGGTGCGCC 
     3601 AGACACTGGG CCAACTTCCT GCTGGCTTCG CCCAATACTT CACACAGCGC TTTCCCAGGC 
     3661 TGCTACTGCA CACTCACCGT GCCATGAGGA CCTGTGCTTC TGAGAGCCTC TTCCTGCCCT 
     3721 ACTATCCAGC AGCATTGGAA GCCAGGGGCT CCTGCCAGGT GCCACAAAGA GCGGAGACAC 
     3781 CCAGAGGCCC CAAAGGCAGA CCAAGGAGTA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     3841 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     3901 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     3961 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4021 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4081 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4141 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4201 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     4261 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     4321 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     4381 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     4441 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     4501 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     4561 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     4621 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     4681 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     4741 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     4801 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     4861 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     4921 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     4981 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     5041 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     5101 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     5161 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     5221 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     5281 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     5341 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     5401 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     5461 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     5521 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     5581 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     5641 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     5701 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     5761 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     5821 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     5881 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     5941 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6001 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6061 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     6121 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     6181 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     6241 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     6301 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     6361 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     6421 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     6481 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     6541 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     6601 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     6661 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     6721 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     6781 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     6841 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     6901 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     6961 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7021 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7081 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     7141 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     7201 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     7261 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     7321 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     7381 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     7441 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     7501 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     7561 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     7621 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
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