LOCUS       RR209345                8139 bp    DNA     circular  SYN 
DEFINITION  Nnt (Myc-DDK-tagged ORF) - Rat nicotinamide nucleotide transhydrogenase (Nnt), nuclear gene encoding mitochondrial protein, (10 ug)
ACCESSION   NM_001013157
VERSION     NM_001013157.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4296..4387 
                     /label="Myc-DDK"  
     misc_feature    4417..5002 
                     /label="PolyA signal"  
     misc_feature    4474..4493 
                     /label="Restriction Enzyme"  
     misc_feature    5278..5950 
                     /label="ColE1"  
     misc_feature    6380..7174 
                     /label="Kan/Neo"  
     misc_feature    7209..7511 
                     /label="SV40 ori"  
     misc_feature    7597..8074 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4278..4284 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4278 
                     /label="Nnt"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCACATCTA CTGAAAACGG TGGTGACTGG CTGTTTATGT CCTTCTGTTA 
     1081 GCAATTTGGG GTCCTGTAAG GTTCTACCTG GGAAGAAAGA CTTTTTACGA ACATTTCGTA 
     1141 CCCACCAGGC ACTGTGGTGT AAATCCCCGG CAAAACCAGG AATTCCATAT AAGCAGTTGA 
     1201 CAGTTGGGGT CCCCAAGGAG ATTTTCCAAA ATGAGAAGCG AGTTGCCTTG TCTCCTGCTG 
     1261 GTGTCCAGGC CCTGGTCAAG CAGGGTTTTA ACGTTGTTGT GGAATCAGGA GCAGGCGAAG 
     1321 CTTCCAAATT CCCAGATGAT CTCTACAGAG CAGCAGGGGC CCAGATCCAA GGAACGAAGG 
     1381 AAGTTCTGGC TTCTGATCTG GTGGTCAAAG TGAGGGCCCC CATGGTGAAC CCGACGTTAG 
     1441 GTGCTCATGA AGCTGACTTT TTAAAGCCCT CAGGAACTCT AATTAGCTTC ATTTACCCAG 
     1501 CCCAAAATCC AGACTTGCTA AATAAACTCT CCGAAAGAAA AACTACAGTT TTGGCGATGG 
     1561 ACCAGGTTCC GAGGGTGACA ATAGCTCAGG GCTATGATGC TCTCAGCTCC ATGGCCAACA 
     1621 TTTCTGGTTA TAAGGCTGTT GTTTTAGCAG CCAATCATTT TGGAAGGTTT TTCACTGGTC 
     1681 AGATCACAGC TGCTGGAAAA GTCCCCCCAG CGAAGATCCT GATAGTTGGT GGTGGCGTTG 
     1741 CCGGGCTCGC TTCTGCAGGG GCAGCAAAGT CAATGGGCGC AGTTGTCCGA GGATTTGATA 
     1801 CAAGAGCTGC CGCCTTGGAA CAGTTCAAAT CCCTTGGTGC TGAACCCTTG GAGGTGGACT 
     1861 TGAAGGAGTC TGGTGAGGGC CAAGGAGGAT ACGCGAAGGA GATGTCCAAA GAGTTTATTG 
     1921 AGGCGGAAAT GAAGCTCTTT GCTCAGCAAT GCAAGGAAGT GGACATCCTT ATCAGCACAG 
     1981 CTCTGATTCC AGGTAAGAAA GCTCCTGTTC TATTTAGTAA AGAAATGATC GAGTCCATGA 
     2041 AGGAAGGTTC AGTAGTTGTG GATTTAGCTG CTGAGGCCGG CGGAAACTTT GAAACGACTA 
     2101 AACCGGGAGA ACTTTACGTT CATAAGGGAA TCACTCATAT TGGCTACACA GACCTCCCCA 
     2161 GCCGCATGGC CACTCAGGCT AGCACTCTGT ACTCCAACAA CATCACCAAG CTCCTGAAGG 
     2221 CCATCAGCCC CGACAAGGAC AATTTTCACT TTGAAGTGAA GGATGACTTT GACTTTGGTA 
     2281 CAATGAGTCA CGTCATTCGA GGAACCGTGG TGATGAAGGA TGGCAATGTG ATTTTCCCAG 
     2341 CTCCCACACC AAAAAATATT CCCAAAGAAG CCCCAGCCAA ACAGAAGACC GTGGCTGAGC 
     2401 TGGAAGCAGA GAAGGCAGGC ACTGTCTCAA TGTACACGAA GACACTGAGG ACAGCATCCG 
     2461 TGTATTCAGC AGGTCTCACC GGGATGCTAG GTTTGGGCAT CGTGGCCCCC AATCTAGCCT 
     2521 TTTCTCAGAT GGTGACCACC TTTGGCCTGG CGGGCATTAT TGGTTACCAC ACTGTCTGGG 
     2581 GAGTGACCCC TGCTCTCCAC TCACCACTGA TGTCTGTGAC CAATGCTATC TCAGGTTTGA 
     2641 CTGCAGTTGG TGGGCTGGCG TTGATGGGAG GGCATTTTTA TCCCTCCACA ACTTCCCAGA 
     2701 GTCTTGCTGC TCTTGCTACA TTCATATCGT CAGTCAACAT TGCAGGTGGT TTTCTGGTGA 
     2761 CTCAGAGAAT GTTGGACATG TTCAAGCGAC CCACGGACCC TCCAGAATAC AATTACCTGT 
     2821 ATCTGCTCCC TGGCGGCACC TTCGTGGGGG GATATTTAGC AGCTCTCTAC GGTGGTTATA 
     2881 ACATCGAAGA AATCATGTAC CTGGGCTCAG GTTTGTGCTG TGTTGGGGCC CTGGGCGGCC 
     2941 TTTCCACTCA GGGAACAGCT CGACTCGGCA ATGCCTTGGG CATGATTGGG GTTGCAGGAG 
     3001 GCCTGGCAGC TACCCTTGGA GGCCTGAAGC CGGACCCACA GCTGTTAGCT CAGATGTCCG 
     3061 GAGCAATGGC GATGGGCGGC ACCATCGGAC TGGCGATTGC AAAGCGGATC CAGATCTCCG 
     3121 ACCTGCCTCA ACTGGTTGCT GCTTTCCACA GTTTAGTGGG TCTGGCGGCA GTGCTCACAT 
     3181 GCATGGCCGA GTACATCGTG GAGTATCCAC ATTTCGCAAT GGATGCCACT TCAAATTTCA 
     3241 CCAAGATTGT GGCCTACATT GGCACTTACA TCGGCGGAGT CACCTTTAGT GGGTCTCTGG 
     3301 TTGCCTACGG AAAATTACAG GGTATTCTGA AGTCAGCCCC TCTGCTGCTC CCTGGAAGGC 
     3361 ACGCGCTCAA TGCAGGCTTG CTGGCTGCTA GCGTGGGGGG AATAATCCCA TTTATGGCTG 
     3421 ACCCTAGTTT TACCACAGGC ATCATGTGTC TGGGCTCTGT GTCCGCACTC TCTACTCTGA 
     3481 TGGGTGTGAC CTTGACAGCT GCCATTGGCG GTGCTGACAT GCCTGTTGTT ATCACTGTGC 
     3541 TAAACAGCTA CTCCGGCTGG GCTCTGTGTG CGGAGGGCTT CCTGCTCAAC AACAACCTGT 
     3601 TGACCATTGT GGGAGCACTC ATCGGCTCCT CGGGCGCCAT CTTGTCGTAC ATCATGTGTG 
     3661 TGGCAATGAA CCGTTCCCTG GCAAATGTAA TTCTCGGAGG CTATGGTACG ACTTCAACAG 
     3721 CTGGTGGAAA ACCCATGGAA ATTTCCGGCA CACATACAGA AATCAACCTG GACAATGCAG 
     3781 TCGAGATGAT CCGGGAAGCT AACAGCATTG TTATTACTCC AGGATATGGG CTCTGTGCAG 
     3841 CTAAAGCTCA GTACCCCATT GCCGATTTGG TGAAGATGCT TACCGAACAA GGCAAGAAAG 
     3901 TCAGGTTTGG AATCCACCCA GTTGCGGGCC GAATGCCTGG TCAGCTTAAT GTGCTCCTGG 
     3961 CCGAGGCAGG AGTGCCCTAT GATATTGTGC TAGAAATGGA TGAGATCAAC AGTGATTTCC 
     4021 CAGATACCGA TCTGGTTCTT GTCATTGGAG CTAATGACAC CGTGAATTCA GCGGCTCAGG 
     4081 AAGACCCCAA TTCTATTATT GCAGGCATGC CAGTCCTCGA GGTCTGGAAG TCCAAACAGG 
     4141 TCATTGTCAT GAAGAGGTCC CTTGGTGTTG GCTACGCTGC AGTGGACAAT CCAATCTTCT 
     4201 ACAAACCTAA CACAGCCATG CTTCTGGGGG ACGCCAAGAA GACATGTGAT GCTCTGCAGG 
     4261 CCAAAGTTCG AGAATCCTAT CAGAAGACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4321 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4381 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4441 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4501 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4561 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4621 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4681 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4741 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     4801 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     4861 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     4921 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     4981 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5041 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5101 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5161 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5221 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5281 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5341 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5401 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5461 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     5521 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     5581 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     5641 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     5701 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     5761 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     5821 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     5881 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     5941 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     6001 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6061 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6121 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6181 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6241 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6301 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6361 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6421 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6481 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6541 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     6601 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     6661 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     6721 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     6781 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     6841 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     6901 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     6961 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     7021 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7081 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7141 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7201 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7261 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7321 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7381 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7441 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7501 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7561 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     7621 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     7681 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     7741 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     7801 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     7861 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     7921 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     7981 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     8041 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8101 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//