LOCUS       RR209318                8424 bp    DNA     circular  SYN 
DEFINITION  Lars (Myc-DDK-tagged ORF) - Rat leucyl-tRNA synthetase (Lars), (10 ug)
ACCESSION   NM_001009637
VERSION     NM_001009637.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4579..4670 
                     /label="Myc-DDK"  
     misc_feature    4700..5285 
                     /label="PolyA signal"  
     misc_feature    4757..4776 
                     /label="Restriction Enzyme"  
     misc_feature    5561..6233 
                     /label="ColE1"  
     misc_feature    6663..7457 
                     /label="Kan/Neo"  
     misc_feature    7492..7794 
                     /label="SV40 ori"  
     misc_feature    7880..8357 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4554..4561 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4554 
                     /label="Lars"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGAAAGA AAAGGAACAG CCAAAGTAGA CTTCTTGAAG AAGATTGAGA 
     1081 AAGAAGTCCA GCAGAAATGG GAAGCGGAGA AAGTGTTTGA AGTCAGCGCG TCTTCACTGG 
     1141 AGAAGCAGAA GCAGAGCAGC AAGGGCAAAT ACTTTGTCAC CTTTCCATAT CCATACATGA 
     1201 ATGGACGCCT TCATTTGGGA CACACGTTTT CCTTATCCAA GTGTGAGTTT GCAGTAGGAT 
     1261 ACCAGAGATT AAAAGGAAAA AGCTGTCTGT TCCCCTTCGG GTTACACTGT ACCGGTATGC 
     1321 CTATTAAGGC ATGTGCTGAC AAGCTGAAAA GGGAGATAGA GCTGTATGGC TGCCCTCCTG 
     1381 ATTTTCCAGA GGAGGAGGAG GAAGAGGAAG AAAGCAGTGC TAAACCTGGA GATATAGTAA 
     1441 TGAAGGATAA AGCCAAAGGG AAAAAGAGTA AAGCCGCTGC TAAAGCAGGA TCTTCTAAGT 
     1501 ACCAGTGGAA CATTATGAAA TCCCTTGGGC TCTCTGATGA AGACATCGCG AAGTTTTCTG 
     1561 AAGCAGAACA TTGGCTTGAT TATTTCCCAC CACTGGCTGT TCAGGATCTG AAGAAAATAG 
     1621 GTTTGAAGGT AGACTGGCGG CGTTCCTTCA TCACCACGGA TGTGAATCCT TACTATGATT 
     1681 CTTTTGTCAG GTGGCAATTT TTAACTTTAA GAGAAAGAAA TAAAATTAAA TTCGGGAAGC 
     1741 GGTACACTAT TTATTCCCCA AAAGATGGAC AGCCTTGCAT GGATCATGAC AGACAAACCG 
     1801 GAGAGGGTGT TGGACCTCAG GAATATACCC TAGTCAAACT GAAGGTGCTT GATCCATACC 
     1861 CATCAAAATT AAGTGGCCTG AAAGGGAAAA ATATCTTCCT AGTAGCTGCC ACACTCAGAC 
     1921 CTGAGACTAT GTTTGGACAG ACAAATTGTT GGGTTCGGCC TGATATGAAA TACATTGGGT 
     1981 TTGAGACAGC AAATGGAGAC ATATTCATCT GTACCCAGAG AGCAGCCAGG AATATGTCAT 
     2041 ACCAAGGCTT CACCAAGCAC AATGGCGTGG TGCCGGTTGT GAAGGAGTTA ATGGGAGAGG 
     2101 AAATCCTTGG GGCTTCACTT TCTGCACCCC TGACATGCTA TAAGGTGATC TATGTTCTCC 
     2161 CTATGCTGAC CATTAAGGAG GATAAAGGCA CTGGGGTGGT CACAAGCGTT CCTTCTGATT 
     2221 CCCCGGATGA TTTTGCGGCA CTCAGAGACT TGAAGAAAAA GCAAGCCTTA CGAGCAAAGT 
     2281 ATGCAGTCAG AGATGACATG GTCCTGCCCT TTGAACCGGT GCCAGTCCTT GAAATCCCAG 
     2341 GGATTGGAAA TCTTCCTGCT GTGACCGTTT GTGACGAACT GAAAATTCAG AGCCAGAATG 
     2401 ACCGTGAAAA ACTTGCAGAG GCAAAGGAGA AATTGTATCT AAGAGGATTT TATGATGGTG 
     2461 TAATGCTAGT GGATGGATTT AAAGGGCAGA AGATCCAACA TGTAAAGAAG ACTATTCAGA 
     2521 AAAACATGAT TGATGCTGGA GATGCGCTTA TCTACATGGA GCCAGAAAAA CAAGTGGTGT 
     2581 CGAGGTCTGC AGATGAGTGT GTTGTGGCCC TGTGTGACCA ATGGTACTTG GATTATGGAG 
     2641 ATGAGAACTG GAAGAAACAG ACATTTCAGT GCCTGAAGAA CATGGAAACA TTCTGCGAGG 
     2701 AGAGCAGGAA GAATTTTGAA GCTTCCTTAG ACTGGCTGCA AGAACATGCC TGCTCAAGAA 
     2761 CTTACGGTTT AGGCACGCGT CTGCCTTGGG ATGAGCAGTG GTTGATTGAA TCCCTCTCAG 
     2821 ACTCCACTAT TTACATGGCG TTTTACACAG TTGCACACCT GTTGCAGGGG GGTGACCTGC 
     2881 AGGGTCAGGC GGAGTCTCCA CTGGGCATCA GACCCCAGCA GATGACGAGG GATGTTTGGG 
     2941 ATTATGTGTT CTTCAAGGAT GCTCCATTTC CCAAGACTCA GATCCCAAAG GAAAAGCTGG 
     3001 ATCAGCTGAA GCAGGAATTT GAGTTTTGGT ATCCAGTGGA CCTTCGAGCC TCTGGCAAGG 
     3061 ATCTGATTCC AAATCATCTT TCATATTACA TTTACAATCA TGTGGCCATG TGGCCAGAAC 
     3121 AAAGCGACAA GTGGCCTGTA TCCGTTCGAG CAAATGGACA CCTGCTGCTC AACTCCGAGA 
     3181 AGATGTCAAA ATCTACGGGA AACTTCCTCA CTTTGAGCCA AGCTGTTGAC AAGTTTTCCG 
     3241 CTGATGGAAT GCGCCTGGCT CTGGCTGATG CTGGCGACAC TGTTGAAGAT GCTAACTTTG 
     3301 TGGAAGCTAT GGCAGACGCA GGCATTCTCC GTCTGTACAC ATGGGTAGAG TGGGTCAAAG 
     3361 AAATGCTTGC CAACTGCAGC AGCCTGAGAA GTGGACCTGC CAACTCATTC AATGATCGTG 
     3421 TGTTTGCCAG TGAAATGAAT GCAGGAATTA TAAAAACAGA TCAAAACTAT GAAAAGATGA 
     3481 TGTTTAAAGA AGCGTTGAAA ACAGGTTTTT TTGAGTTTCA GGCTGCGAAA GACAAGTACC 
     3541 GTGAACTGGC CACAGAGGGG ATGCACAGAG AGCTTGTGTT CCGGTTTATT GAAGTTCAAA 
     3601 CAATCCTTCT GACTCCATTC TGTCCACATT TGTGTGAGCA CATCTGGACA CTGCTGGGAA 
     3661 AGCCTGATTC AATTATGCAT GCCTCATGGC CTGTGGCAGG TCCCGTGGAT GAATCCCTGA 
     3721 TTCGTTCCTC ACAGTACCTC ATGGAAGTAG CACACGACCT TAGATTACGG CTCAAGAATT 
     3781 ACATGACGCC AGCTAAAGGG AAGAAGACGG ACAAGCAACC AGTTCAGAGG CCCTCACACT 
     3841 GCACCATCTA TGTGGCAAAG AACTACCCTG TCTGGCAGCA CATTACCCTG ACAACCCTAC 
     3901 GGAGCCACTT TGAGGCTAAC AATGGAAAAT TGCCTGATAA CAAAGTCATC GCTTGTGAAC 
     3961 TAGGCAGTTT GCCAGAACTG AAGAAATATA TGAAGAAAGT TATGCCATTT GTTGCCATGA 
     4021 TTAAGGAAAA CATGGAGAAG AAAGGGCCGC GAGTCTTGGA TTTAGAGTTG GAATTTGATG 
     4081 AGCAGGCAGT CCTTATGGAG AATATAGTCT ACCTAACCAA TTCTCTTGAG CTGGAACATA 
     4141 TAGACGTCAA GTTTGCCTCT GAAGCTGAAG ATAAAGTCAG GGAAGAGTGC TGTCCTGGGA 
     4201 AGCCACTTAA TGTTTTCAGA ACAGAACCTG GTGTGCCAGT TTCCCTAGTA AATCCTCAGC 
     4261 CATCCAGCGG GCACTTCTCT ACCAAAATCG ACATCAGACA AGGAGATAGC TGTGAGTCCA 
     4321 TAATCAGACG CCTGACGAAA ATGGACCGAG GAATCAAAGA TCTCTCCAAA GTGAAACTCA 
     4381 TGCGCTTTGA TGATCCTCTG TTGGGGCCTC GACGAGTTCC AGTCCTGGGG AGAGAGCAGA 
     4441 ACGAGAAGAC ACTCATTTCT GAGAATGCCA TTTTCCACGT GGACCTAGTG AGCAAGAAAG 
     4501 TTCACCTTAC TGAGAACGGG CTGCGCGCAG ACATCGGTGA CACAATAGTC TATCTGGTTC 
     4561 ACAGCGGACC GACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4621 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4681 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4741 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4801 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4861 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4921 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4981 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     5041 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5101 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5161 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5221 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5281 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5341 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5401 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5461 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5521 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5581 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5641 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5701 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5761 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5821 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5881 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5941 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     6001 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6061 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6121 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6181 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6241 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6301 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6361 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6421 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6481 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6541 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6601 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6661 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6721 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6781 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6841 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6901 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6961 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     7021 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     7081 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7141 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7201 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7261 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7321 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7381 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7441 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7501 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7561 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7621 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7681 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7741 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7801 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7861 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7921 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7981 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     8041 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     8101 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8161 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8221 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8281 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8341 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8401 TAACAAAAAT TTAACGCGAA TTTT
//