LOCUS       RR209284                8205 bp    DNA     circular  SYN 
DEFINITION  Pde3b (Myc-DDK-tagged ORF) - Rat phosphodiesterase 3B, cGMP-inhibited (Pde3b), (10 ug)
ACCESSION   NM_017229
VERSION     NM_017229.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4362..4453 
                     /label="Myc-DDK"  
     misc_feature    4483..5068 
                     /label="PolyA signal"  
     misc_feature    4540..4559 
                     /label="Restriction Enzyme"  
     misc_feature    5344..6016 
                     /label="ColE1"  
     misc_feature    6446..7240 
                     /label="Kan/Neo"  
     misc_feature    7275..7577 
                     /label="SV40 ori"  
     misc_feature    7663..8140 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4344..4350 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4344 
                     /label="Pde3b"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGGAAGGAC GAGCGCGAGC GGGACACGCC AGCCATGAGG TCCCCGCCGC 
     1081 CGCCGCCGCC GCCGGCCACG GCCACGGCCG CCTCGCCCCC CGAGAGCCTG CGCAACGGCT 
     1141 ACGTGAAGAG CTGCGTGAGC CCGCTGCGGC AGGACCCTCC GCGCAGCTTC TTCTTCCACC 
     1201 TCTGCCGCTT CTGCAACGTG GAGCCGCCGG CGGCCTCGCT CCGCGCCGGG GCACGCCTCT 
     1261 CGCTCGCCGC TCTGGCCGCC TTTGTCCTGG CCGCGCTGCT GGGCGCGGGG CCCGAGCGCT 
     1321 GGGCGGCCGC GGCGACCGGG CTTCGGACGC TGCTGAGCGC TTGCTCGCTC AGCCTCAGCC 
     1381 CGCTCTTCAG CATCGCCTGT GCCTTCTTCT TCCTCACCTG CTTCCTGACG CGCGCGCAGC 
     1441 GCGGCCCGGA CCGCGGCGCC GGCTCCTGGT GGCTGCTGGC GCTGCCTGCC TGCTGCTACC 
     1501 TGGGCGACTT CGCGGCGTGG CAGTGGTGGT CGTGGCTGCG CGGGGAGCCG GCGGCGGCGG 
     1561 CGGCCGGCCG GCTCTGCCTG GTGCTGAGCT GCGTGGGGCT GCTGACGCTC GCGCCTCGCG 
     1621 TGAGGCTGCG GCACGGAGTC CTGGTGCTGC TCTTCGCCGG CCTGGTGTGG TGGGTGTCCT 
     1681 TCTCCGGCCT CGGGGCTCTG CCGCCCGCGC TCAGGCCTCT GCTGTCGTGC CTGGTCGGGG 
     1741 GCGCGGGATG CCTGCTAGCC CTGGGCTTGG ACCACTTCTT TCACGTCCGG GGAGCCTCAC 
     1801 CTCCCCCGCG CTCGGCGAGT ACCGCGGATG AAAAAGTGCC TGTGATCAGA CCCCGGAGGA 
     1861 GGTCCAGCTG CGTGTCGCTG GGAGAAAGCG CGGCCGGTTA CTATGGCAGT GGCAAGATGT 
     1921 TCAGGAGACC GTCGTTGCCT TGTATTTCTC GAGAACAGAT GATTCTCTGG GACTGGGACT 
     1981 TGAAGCAGTG GTGTAAACCT CATTACCAGA ATTCTGGAGG TGGAAATGGA GTTGATCTTT 
     2041 CAGTGCTAAA TGAAGCTCGC AATATGGTGT CAGACCTTCT GATTGACCCA AGCCTTCCCC 
     2101 CACAAGTCAT TTCTTCTTTG CGGAGTATCA GTAGCTTGAT GGGTGCTTTC TCAGGTTCCT 
     2161 GTAGGCCAAA GATTAATTCT TTCACACCAT TTCCTGGATT TTATCCCTGT TCTGAAGTAG 
     2221 AAGATCCAGT AGAGAAAGGC GATCGAAAAC TTCACAAGGG GTTGAGTAGC AAACCCAGTT 
     2281 TCCCGACTGC ACAGCTGAGG AGGAGCTCAG GAGCGTCAGG ATTGCTGACC AGTGAGCACC 
     2341 ACTCACGGTG GGATCGCAGC GGTGGCAAAA GGCCTTACCA AGAACTCAGT GTTTCAAGTC 
     2401 ATGGATGCCA CCTAAATGGG CCTTTTAGTT CAAATCTTAT GACAATTCCA AAGCAGAGGT 
     2461 CATCATCTGT GTCGCTGACG CACCATGCAG GTCTGAGAAG AGCTGGTGCT TTGCCCAGCC 
     2521 CCAGTCTTCT GAATTCTTCA AGCCATGTAC CCGTGTCTGC TGGCTGTCTA ACTAATCGAT 
     2581 CACCCGTAGG ATTTCTTGAT ACCAGTGATT TTCTTACTAA GCCAAGTGTT ACTTTACATA 
     2641 GATCACTGGG CAGTGTATCC AGTGCAGCAG ATTTCCATCA GTACCTTAGG AACTCTGACA 
     2701 GCAGTCTGTG TAGCAGCTGT GGACACCAAA TACTCAAGTA TGTTTCGACA TGTGAACCTG 
     2761 ATGGTACAGA CCACCACAAT GAAAAATCAG GTGAAGAAGA CAGCACTGTT TTCTCAAAAG 
     2821 AACGATTGAA CATTGTGGAA ACTCAAGAAG AAGAGACAGT GAAGGAAGAC TGCAGGGAAT 
     2881 TATTTTTGGA AGGTGATGAT CACCTAATGG AAGAGGCCCA GCAACCGAAT ATTGACCAGG 
     2941 AAGTGTTATT GGACCCGATG TTAGTGGAAG ATTATGATTC ATTAATAGAA AAGATGAGCA 
     3001 ACTGGAACTT TCAGATTTTT GAGCTTGTAG AAAAAATGGG AGAAAAATCA GGAAGGATTC 
     3061 TCAGTCAGGT TATGTATACT TTATTTCAAG ATACTGGTTT ATTGGAAACA TTTAAAATTC 
     3121 CCACTCAAGA ATTTATGAAT TATTTTCGTG CACTAGAAAA TGGCTACCGA GATATTCCAT 
     3181 ATCACAATCG TGTGCATGCC ACAGATGTCC TACATGCTGT TTGGTATTTG ACAACACGAC 
     3241 CAATTCCTGG CTTACAGCAG CTCCATAATA ACCATGAAAC AGAAACCAAA GCAGATTCAG 
     3301 ATGCTAGACT TAGTTCTGGA CAGATTGCTT ACCTTTCTTC GAAGAGTTGC TGTATTCCAG 
     3361 ATAAGAGTTA TGGCTGCCTG TCTTCAAACA TTCCTGCGTT AGAACTGATG GCTTTATATG 
     3421 TGGCAGCTGC CATGCACGAT TATGATCACC CAGGAAGAAC AAATGCATTC CTAGTGGCTA 
     3481 CAAATGCACC TCAGGCAGTT TTATACAATG ACAGATCTGT TCTAGAAAAT CATCATGCCG 
     3541 CATCAGCGTG GAATCTGTAT CTTTCTCGCC CAGAATACAA CTTCCTCCTT AACCTTGATC 
     3601 ACATGGAATT CAAGCGTTTT CGATTTTTAG TCATAGAAGC AATCCTTGCT ACAGATCTCA 
     3661 AAAAACATTT TGATTTCCTT GCTGAATTCA ATGCCAAGGC CAATGATGTA AATAGTAACG 
     3721 GTATAGAATG GAGCAGTGAA AACGATCGAC TCTTAGTCTG CCAGGTGTGC ATCAAATTAG 
     3781 CAGACATCAA CGGCCCAGCA AAAGATCGGG ATCTTCATTT GAGATGGACA GAAGGCATTG 
     3841 TGAATGAGTT TTATGAGCAG GGAGATGAAG AAGCAACCCT GGGTCTACCT ATTAGTCCCT 
     3901 TCATGGATCG TTCTTCTCCT CAACTAGCAA AACTCCAAGA GTCATTTATC ACCCACATTG 
     3961 TGGGCCCCCT GTGCAACTCC TATGATGCTG CTGGCTTGCT GCCTGGTCAG TGGATAGAAG 
     4021 CAGAAGAGGG TGACGATACA GAAAGTGATG ATGATGATGA TGATGATGAT GATGATGATG 
     4081 ATGATGATGA TGAAGAGTTA GATTCAGATG ATGAAGAAAC AGAAGACAAT CTAAATCCTA 
     4141 AACCACAAAG AAGGAAAGGC AGGCGGCGAA TATTTTGCCA ACTAATGCAC CACCTCACTG 
     4201 AAAACCACAA GATCTGGAAG GAAATCATAG AAGAGGAAGA AAAATGTAAA GCTGAGGGGA 
     4261 ACAAACTGCA GGTGGATAAT GCCTCCTTAC CTCAGGCAGA TGAGATTCAG GTCATTGAAG 
     4321 AAGCAGATGA AGAGGAAGAA CAAATGTTTG AAACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4381 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4441 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4501 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4561 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4621 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4681 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4741 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     4801 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     4861 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     4921 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     4981 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5041 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5101 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5161 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5221 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5281 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5341 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5401 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5461 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     5521 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     5581 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     5641 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     5701 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     5761 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     5821 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     5881 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     5941 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6001 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6061 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6121 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6181 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6241 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6301 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6361 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6421 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6481 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6541 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     6601 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     6661 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     6721 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     6781 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     6841 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     6901 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     6961 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7021 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7081 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7141 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7201 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7261 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7321 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7381 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7441 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7501 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7561 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     7621 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7681 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     7741 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     7801 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     7861 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     7921 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     7981 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8041 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8101 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8161 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//