LOCUS       RR209270                8943 bp    DNA     circular  SYN 
DEFINITION  Tnik (Myc-DDK-tagged ORF) - Rat similar to Traf2 and NCK interacting kinase, splice variant 4 (RGD1561817), (10 ug)
ACCESSION   NM_001106422
VERSION     NM_001106422.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5100..5191 
                     /label="Myc-DDK"  
     misc_feature    5221..5806 
                     /label="PolyA signal"  
     misc_feature    5278..5297 
                     /label="Restriction Enzyme"  
     misc_feature    6082..6754 
                     /label="ColE1"  
     misc_feature    7184..7978 
                     /label="Kan/Neo"  
     misc_feature    8013..8315 
                     /label="SV40 ori"  
     misc_feature    8401..8878 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5082..5088 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5082 
                     /label="Tnik"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGAGCGAC TCCCCAGCTC GCAGCCTGGA TGAAATCGAT CTCTCCGCCC 
     1081 TGAGGGACCC TGCAGGGATC TTTGAGTTGG TGGAACTTGT CGGAAATGGT ACATATGGCC 
     1141 AAGTTTATAA GGGTCGTCAT GTCAAAACAG GCCAGCTTGC TGCCATTAAG GTTATGGACG 
     1201 TCACAGGGGA TGAAGAGGAA GAAATCAAAC AAGAAATTAA CATGTTGAAG AAATATTCTC 
     1261 ATCACAGGAA CATTGCTACA TACTACGGTG CTTTTATCAA AAAGAACCCT CCGGGCATGG 
     1321 ATGACCAACT ATGGTTGGTT ATGGAGTTCT GTGGTGCTGG CTCTGTCACT GACCTGATCA 
     1381 AGAACACGAA AGGCAACACA TTGAAAGAGG AGTGGATTGC ATACATCTGC AGGGAGATCT 
     1441 TACGGGGCCT GAGTCACCTG CACCAGCACA AAGTGATTCA TCGGGATATT AAAGGACAGA 
     1501 ATGTCTTGCT GACTGAAAAT GCAGAAGTTA AGCTAGTGGA TTTTGGCGTG AGTGCCCAGC 
     1561 TTGACCGAAC AGTGGGCAGG AGGAACACGT TCATCGGGAC CCCCTACTGG ATGGCACCCG 
     1621 AAGTCATTGC CTGTGATGAG AACCCAGATG CCACGTATGA TTTCAAGAGT GACTTGTGGT 
     1681 CTTTGGGAAT CACCGCCATT GAGATGGCAG AAGGTGCCCC CCCCCTCTGT GATATGCATC 
     1741 CCATGAGAGC GCTCTTCCTC ATCCCACGGA ACCCCGCACC TCGCCTCAAG TCTAAGAAGT 
     1801 GGTCAAAAAA ATTCCAGTCA TTTATCGAGA GCTGCTTGGT AAAGAATCAC GGCCAGCGGC 
     1861 CAGCCACAGA ACAGTTGATG AAGCACCCAT TCATACGAGA CCAGCCTAAT GAGAGGCAGG 
     1921 TCCGCATCCA GCTGAAGGAC CACATTGATC GAACAAAGAA GAAGCGAGGA GAAAAAGATG 
     1981 AGACTGAGTA TGAATACAGT GGAAGTGAGG AAGAAGAGGA AGAGAACGAC TCTGGGGAAC 
     2041 CCAGCTCCAT TCTGAACCTA CCAGGAGAGT CAACACTGCG ACGGGACTTC CTGAGACTGC 
     2101 AGCTGGCCAA CAAGGAGCGC TCAGAGGCCC TGCGGCGCCA ACAGCTGGAG CAGCAGCAGA 
     2161 GGGAGAATGA AGAACATAAG AGGCAGCTAC TGGCTGAGCG CCAGAAGCGC ATCGAGGAGC 
     2221 AGAAGGAGCA GAGGCGGAGG CTGGAGGAGC AACAAAGGCG AGAAAAGGAG CTTCGGAAGC 
     2281 AGCAGGAGCG GGAACAGCGC CGGCACTATG AGGAGCAGAT GCGCCGGGAG GAGGAGAGGA 
     2341 GGCGCGCAGA ACACGAACAG GAGTACATCA GGCGACAGCT AGAAGAAGAG CAAAGGCAGT 
     2401 TAGAAATCTT ACAGCAGCAG CTACTGCATG AGCAAGCTCT ACTTCTGGAA TATAAGCGCA 
     2461 AACAACTGGA AGAACAGAGA CAAGCAGAAA GACTGCAGCG GCAGCTAAAG CAAGAGCGGG 
     2521 ACTATCTGGT TTCCCTCCAG CACCAGCGGC AGGAGCAGAG GCCCCTGGAG AAGAAGCCAT 
     2581 TGTACCATTA CAAGGAGGGC ATGAGTCCTA GTGAGAAGCC TGCCTGGGCC AAGGAGGTGG 
     2641 AAGAACGCTC AAGACTCAAC CGACAGAGTT CACCTGCCAT GCCTCACAAG GTTGCCAACA 
     2701 GGATCTCAGA CCCCAACCTA CCCCCAAGAT CAGAGTCCTT CAGCATTAGT GGGGTTCAGC 
     2761 CTGCTCGGAC ACCCCCAATG CTCAGACCTG TTGATCCCCA GATCCCGCAG CTGGTAGCCG 
     2821 TCAAATCCCA GGGACCTGCC TTGACCGCCT CCCAGTCAGT GCATGAGCAA CCCACAAAAG 
     2881 GCCTGTCTGG GTTCCAGGAG GCTCTGAATG TGACCTCTCA CCGGGTCGAG ATGCCACGCC 
     2941 AGAACTCGGA TCCCACCTCG GAAAACCCTC CTCTCCCCAC GCGCATTGAG AAGTTTGACC 
     3001 GAAGCTCTTG GTTACGACAG GAAGAAGACA TTCCACCAAA GGTGCCTCAA AGAACAACTT 
     3061 CTATATCCCC AGCACTAGCC AGAAAGAATT CCCCTGGGAA TGGCAGTGCT TTAGGCCCAA 
     3121 GACTCGGATC TCAGCCTATC AGAGCAAGCA ACCCTGATCT CCGCAGGACA GAGCCAGTCC 
     3181 TGGAGAGCTC CTTGCAGCGG ACCAGCAGTG GCAGTTCTTC CAGCTCCAGC ACTCCCAGCT 
     3241 CCCAGCCCAG CTCTCAAGGA GGCTCTCAGC CTGGGTCACA AGCAGGATCT AGTGAACGAA 
     3301 CCAGAGTTCG GGCCAACAGT AAGTCAGAAG GATCGCCCGT GCTCCCCCAT GAGCCTTCCA 
     3361 AGGTGAAACC AGAAGAATCC AGAGACATCC CACGGCCCAG CCGGCCAGCT GATCTGACGG 
     3421 CGTTAGCCAA AGAATTAAGA GAACTCCGGA TTGAAGAAAC AAACCGCCCA CTGAAGAAAG 
     3481 TGACTGATTA CTCTTCCTCC AGCGAGGAGT CAGAGAGCAG TGAGGAAGAA GAGGAGGATG 
     3541 GAGAGAGTGA GACGCACGAT GGGACAGTGG CTGTCAGTGA CATACCCAGA CTAATACCCA 
     3601 CGGGTGCTCC AGGGAAGAAT GAGCAGTACA ACATGGGGAT GGTCGGCACA CATGGGCTGG 
     3661 AGACTTCGCA TGCAGATACC TTTGGCGGCA GCATTTCAAG AGAAGGAACC TTGATGATCA 
     3721 GAGAGACGGC TGAAGAGAAG AAGCGATCTG GCCACAGTGA CAGTAATGGC TTCGCAGGTC 
     3781 ACATCAATCT CCCAGACCTT GTGCAGCAGA GCCATTCGCC AGCTGGGACT CCCACTGAGG 
     3841 GCCTGGGCCG TGTCTCCACT CATTCCCAGG AGATGGACAC TGGAGCTGAA TATGGTATGG 
     3901 GAAGCAGCAC CAAAGCCTCC TTCACCCCCT TCGTGGACCC TAGAGTGTAC CAGACATCGC 
     3961 CCACTGATGA AGATGAAGAG GATGATGAGT CTTCGGCTGC TGCCCTGTTT ACTAGCGAAC 
     4021 TTCTTAGGCA AGAACAGGCC AAACTCAATG AAGCGAGGAA GATTTCAGTG GTAAATGTGA 
     4081 ACCCAACGAA CATTCGCCCT CACAGTGACA CACCGGAAAT CAGAAAATAC AAGAAACGAT 
     4141 TCAATTCCGA AATACTCTGT GCAGCTCTGT GGGGTGTGAA CCTTCTGGTG GGGACTGAAA 
     4201 ATGGCCTGAT GCTTTTGGAC AGAAGCGGCC AAGGCAAAGT CTACAACCTC ATCAACCGGA 
     4261 GGCGATTTCA GCAGATGGAC GTGCTAGAAG GACTAAACGT TCTTGTCACG ATATCAGGAA 
     4321 AGAAGAATAA GCTCCGGGTG TACTATCTCT CGTGGTTAAG AAACAGAATC CTGCACAATG 
     4381 ACCCAGAAGT AGAAAAGAAG CAGGGTTGGA TCACCGTCGG AGACTTGGAA GGCTGTATCC 
     4441 ATTACAAAGT TGTTAAATAT GAAAGGATCA AGTTCTTGGT GATTGCCTTA AAGAATGCAG 
     4501 TAGAGATATA CGCATGGGCG CCTAAACCTT ATCATAAGTT CATGGCATTT AAGTCTTTTG 
     4561 CAGATCTTCA GCATAAGCCT CTGCTCGTTG ACCTCACAGT AGAAGAAGGT CAAAGGTTAA 
     4621 AGGTCATTTT TGGCTCACAC ACTGGTTTCC ATGTAATTGA TGTTGACTCT GGAAACTCCT 
     4681 ACGATATCTA TATACCATCC CATATTCAGG GTAATATCAC TCCTCACGCT ATCGTCATCT 
     4741 TGCCTAAAAC AGACGGAATG GAGATGCTTG TCTGCTATGA GGATGAGGGG GTGTATGTGA 
     4801 ACACCTATGG CCGGATCACT AAGGATGTGG TGCTCCAATG GGGAGAAATG CCCACATCTG 
     4861 TGGCCTACAT TCATTCCAAT CAGATAATGG GCTGGGGCGA GAAAGCTATT GAGATCCGGT 
     4921 CAGTGGAAAC AGGACATTTG GATGGAGTGT TTATGCATAA ACGAGCTCAA AGGTTAAAGT 
     4981 TTCTATGTGA AAGAAATGAT AAGGTAAATC CGTTTCAACT TTGCTTTAGT GTTTGCATTT 
     5041 TAAGAGACCA CCAGGTACCT GTGAAACCTT CATTTTCCTT TATACTGACT ACGCGTACGC 
     5101 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     5161 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     5221 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     5281 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     5341 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     5401 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     5461 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     5521 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     5581 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     5641 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     5701 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5761 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5821 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     5881 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     5941 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     6001 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     6061 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     6121 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     6181 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     6241 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     6301 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     6361 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     6421 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     6481 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     6541 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     6601 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     6661 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     6721 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     6781 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     6841 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     6901 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     6961 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     7021 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     7081 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     7141 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     7201 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     7261 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     7321 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     7381 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     7441 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     7501 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     7561 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     7621 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     7681 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     7741 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     7801 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     7861 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     7921 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     7981 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     8041 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     8101 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     8161 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     8221 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     8281 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     8341 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     8401 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     8461 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     8521 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     8581 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     8641 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     8701 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     8761 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     8821 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     8881 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     8941 TTT
//