LOCUS       RR209260                7272 bp    DNA     circular  SYN 
DEFINITION  Ganab (Myc-DDK-tagged ORF) - Rat glucosidase, alpha, neutral AB (Ganab), (10 ug)
ACCESSION   NM_001106334
VERSION     NM_001106334.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3429..3520 
                     /label="Myc-DDK"  
     misc_feature    3550..4135 
                     /label="PolyA signal"  
     misc_feature    3607..3626 
                     /label="Restriction Enzyme"  
     misc_feature    4411..5083 
                     /label="ColE1"  
     misc_feature    5513..6307 
                     /label="Kan/Neo"  
     misc_feature    6342..6644 
                     /label="SV40 ori"  
     misc_feature    6730..7207 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3411..3417 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3411 
                     /label="Ganab"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCCGTGGAC TTATGGCTTT TGAGCATCAG AGGGCTCCCA GGGTCCCTTT 
     1081 CTCGGATAAA GTTAGTCTCA CGCTCGGTAG CATGTGGGAT AAGATCAAGA ACCTTTTCTC 
     1141 TAGGCAAGAA CCAAAAGATG CAGCTGAAGG CAACGGAGCC GAGCCTGAAG CAAGGCCTGG 
     1201 GGATGGTGAC AAGGCAACCA GAGGAGACCC AGGAAAAGGC TGAGAAAGAT GAGCCAGGAG 
     1261 CCTGGGAGGA GACATTCAAA ACACACTCTG ACAGCAAGCC TTACGGCCCC ACGTCGGTAG 
     1321 GTTTGGACTT TTCTCTGCCA GGAATGGAAC ATGTGTATGG GATCCCTGAG CATGCAGACA 
     1381 GCCTGAGACT GAAGGTCACT GAGGGTGGTG AGCCGTACCG TCTGTACAAC TTGGATGTGT 
     1441 TCCAGTATGA GCTGAACAAC CCCATGGCTT TGTATGGGTC TGTGCCTGTG CTCTTGGCAC 
     1501 ACAGCTTTCA TCGGGACTTG GGTATCTTCT GGCTTAATGC TGCTGAGACA TGGGTTGATA 
     1561 TATCGTCCAA CACAGCTGGG AAGACCCTGT TTGGGAAGAT GCTGGATTAC CTGCAGGGGT 
     1621 CTGGGGAGAC TCCACAGACA GACATTCGTT GGATGTCCGA GAGCGGCATT GTTGATGTTT 
     1681 TCCTCATGCT CGGCCCTTCA GTCCTTGATG TCTTCCGGCA GCATGCTAGC CTTACAGGGA 
     1741 CCCAGGCACT ACCCCCACTC TTCTCCCTGG GCTACCACCA GAGTCGCTGG AACTACCGGG 
     1801 ATGAGGCTGA TGTTTTGGAA GTGGATCAGG GTTTTGATGA TCACAACATG CCTTGTGATG 
     1861 TGATATGGTT GGACATTGAA CATGCTGATG GCAAGCGGTA CTTCACTTGG GACCCCACCC 
     1921 GATTTCCTCA GCCCCTCAAT ATGCTTGAGC ACTTGGCTTC TAAAAGGCGG AAGCTGGTGG 
     1981 CCATCGTGGA TCCCCACATC AAGGTAGACT CTGGTTACCG AGTTCATGAA GAATTGCGAA 
     2041 ACCATGGGCT GTATGTTAAA ACTCGGGATG GCTCTGATTA CGAGGGCTGG TGCTGGCCAG 
     2101 GTTCGGCTAG TTACCCTGAC TTCACTAACC CACAGATGAG GGCCTGGTGG GCTAACATGT 
     2161 TCAACTTTCA CAACTATGAG GGTTCAGCTC CTAATCTTTT TGTCTGGAAT GACATGAATG 
     2221 AACCGTCTGT GTTCAATGGT CCTGAAGTCA CCATGCTGAA GGATGCTGTG CATTATGGCG 
     2281 GCTGGGAGCA CCGGGACATC CATAACATCT ATGGCTTATA TGTGCACATG GCAACTGCTG 
     2341 ACGGGCTAAT ACAGCGCTCT GGTGGCATAG AGCGGCCCTT TGTCCTGAGT AGGGCCTTCT 
     2401 TCTCAGGCTC CCAGCGCTTT GGAGCTGTGT GGACAGGGGA CAACACTGCA GAGTGGGATC 
     2461 ATTTGAAGAT CTCTATCCCT ATGTGTCTCA GCCTGGCACT GGTGGGGCTT TCCTTCTGTG 
     2521 GCGCGGATGT GGGTGGCTTC TTCAAGAACC CAGAGCCAGA ACTGCTTGTG CGCTGGTACC 
     2581 AAATGGGTGC CTACCAGCCA TTCTTTCGGG CTCATGCTCA CTTGGACACT GGGCGGCGAG 
     2641 AGCCGTGGCT GTTAGCGTCT CAATACCAAG ATGCAATCCG AGATGCCTTG TTCCAGCGAT 
     2701 ACTCTTTGCT GCCCTTCTGG TATACCCTCT TCTATCAAGC TCACCAGGAA GGGTTTCCTG 
     2761 TCATGAGGCC CCTCTGGGTA CAGTATCCTG AGGATATGTC TACCTTCAGT ATGGAGGATC 
     2821 AGTTCATGCT TGGGGACGCA CTCCTTATTC ACCCTGTGTC GGATGCTGGG GCCCATGGAG 
     2881 TGCAGGTCTA TTTGCCTGGC GAAGAAGAGG TGTGGTATGA CATTCAAAGC TATCAGAAGC 
     2941 ATCATGGTCC CCAGACCTTG TATCTGCCAG TGACCCTGAG CAGTATACCT GTGTTCCAGC 
     3001 GTGGCGGGAC AATTGTCCCT CGATGGATGC GCGTGAGGCG TTCTTCAGAC TGTATGAAGG 
     3061 AAGATCCTAT CACTCTCTTT GTTGCTCTCA GTGCCCAGGG CACGGCCCAA GGAGAGCTCT 
     3121 TTCTCGATGA TGGACACACT TTTAACTATC AGAATCGCCA TGAGTTCCTG TTGCGTCGAT 
     3181 TCTCTTTCTC TGGCAACACA CTTGTCTCTA GTTCAGCAGA CCCCAAAGGC CACCTTGAGA 
     3241 CACCTGTCTG GATTGAGCGA GTGGTCATAA TGGGGGCTGG AAAGCCAGCA GCCGTGGTGC 
     3301 TCCAGACCAA AGGCTCTCCT GAAAGTCGCC TGTCCTTCCA GCATGACCCT GAGACCTCTG 
     3361 TGCTGACATT GCGTAAACCT GGCGTCAATG TGGCATCTGA CTGGAGTATT CATCTTCGAA 
     3421 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     3481 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     3541 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     3601 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     3661 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     3721 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     3781 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     3841 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     3901 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     3961 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     4021 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     4081 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     4141 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     4201 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     4261 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     4321 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     4381 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     4441 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     4501 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     4561 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     4621 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     4681 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     4741 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     4801 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     4861 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     4921 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     4981 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     5041 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     5101 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     5161 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     5221 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     5281 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     5341 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     5401 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     5461 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     5521 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     5581 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     5641 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     5701 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     5761 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     5821 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     5881 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     5941 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     6001 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     6061 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     6121 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     6181 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     6241 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     6301 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     6361 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     6421 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     6481 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     6541 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     6601 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     6661 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     6721 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     6781 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     6841 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     6901 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     6961 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     7021 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     7081 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     7141 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     7201 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     7261 AACGCGAATT TT
//