LOCUS       RR209256                7794 bp    DNA     circular  SYN 
DEFINITION  Cul4b (Myc-DDK-tagged ORF) - Rat cullin 4B (Cul4b), (10 ug)
ACCESSION   NM_001106951
VERSION     NM_001106951.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3951..4042 
                     /label="Myc-DDK"  
     misc_feature    4072..4657 
                     /label="PolyA signal"  
     misc_feature    4129..4148 
                     /label="Restriction Enzyme"  
     misc_feature    4933..5605 
                     /label="ColE1"  
     misc_feature    6035..6829 
                     /label="Kan/Neo"  
     misc_feature    6864..7166 
                     /label="SV40 ori"  
     misc_feature    7252..7729 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3933..3939 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3933 
                     /label="Cul4b"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCACGTTCA ACTAAGTCTA GAGAAAGAAG AGAGAATGAT GCCAACTCAG 
     1081 AAGATAACAG TTCAGAAACC TCCAATCAAG AGCGCCGAAG GTGTCTCCAA GGCCCGCCCC 
     1141 GTCCTCCCTA TCCACCTCTT TTACCCCCGG TGTTCCCTCC CCCAACCCCC CCACCTCAAG 
     1201 TCCGGCGGAC CCGGGGGCTT CAGGATCTGG GAGCTATGAA AAGTGTCTGC CCAGGCACTT 
     1261 CCGGTTTTTC TTCCCCCAAT CCCTCTGCTG CTTCTGCTGC TGCTCAGGAG GTCAGATCTG 
     1321 CCACTGATGG TAATACCAGC ACCACTCCGC CCACCTCTGC CAAGAAGAGA AAGTTAAACA 
     1381 GCAGCAGCAG CAGTAGCAGC AGTAACAGTA GTAACGAGAG AGAAGACTTT GATTCCACCT 
     1441 CTTCCTCCTC CACCCCTCCA CAACCCAGAG ATTCGGCGTC CCCTTCAACC TCATCCTTCT 
     1501 GCCTCGGGGT TCCAGTGGCC ACTTCCAGCC ACGTACCTAT ACAGAAGAAG CTGCGTTTTG 
     1561 AAGACACCCT GGAGTTTGTA GGGTTTGATA CGAAAATGGC TGAGGAATCT TCCTCCTCAT 
     1621 CTTCCTCATC TTCACCAACT GCTGCAACCT CACAGCAGCA ACAGCAGCAA CTTAAAAATA 
     1681 AGAGTATATT AATTTCTTCG GTGGCTTCAG TGCACCATGC AAACGGCCTG GCTAAATCTT 
     1741 CTACCACGGT CTCTAGCTTT GCTAACAGCA AGCCTGGCTC AGCTAAGAAG TTAGTGATCA 
     1801 AGAACTTTAA AGATAAGCCT AAATTACCAG AAAACTATAC AGATGAGACA TGGCAAAAGC 
     1861 TAAAAGAAGC AGTGGAAGCC ATTCAGAATA GTACTTCAAT TAAGTACAAT TTAGAAGAAC 
     1921 TCTACCAGGC TGTAGAAAAT CTTTGTTCTT ACAAGATTTC TGCCAATTTG TATAAGCAGC 
     1981 TGAGGCAGAT CTGTGAAGAT CACATCAAAG CACAGATTCA TCAGTTCAGA GAGGATTCAT 
     2041 TGGATAGTGT TCTTTTTCTA AAAAAGATTG ATAGATGCTG GCAAAATCAT TGTAGGCAAA 
     2101 TGATAATGAT CAGGAGCATT TTTTTGTTTC TGGATAGAAC TTATGTTCTT CAGAATTCAA 
     2161 TGCTGCCCTC TATTTGGGAC ATGGGATTAG AGTTATTTAG GGCTCATATT ATAAGTGATC 
     2221 AAAAAGTCCA GACAAAAACA ATCGATGGCA TCCTTCTCTT GATTGAGAGA GAGCGGAATG 
     2281 GTGAAGCAAT TGATAGAAGT TTACTTCGAA GCCTTTTAAA TATGCTGTCT GACTTGCAAA 
     2341 TTTACCAAGA CTCTTTTGAA CAACGATTTT TGCAAGAAAC TAATCGGCTT TATGCGGCCG 
     2401 AAGGTCAGAA GTTAATGCAA GAAAGAGAGG TTCCTGAGTA TCTTCATCAT GTTAATAAAC 
     2461 GCCTAGAAGA AGAAGCAGAC AGACTTATTA CTTACTTAGA TCAGACCACC CAGAAGTCAC 
     2521 TGATTGCTTC TGTTGAAAAA CAACTTCTAG GTGAACACTT AACAGCAATT CTTCAGAAAG 
     2581 GTTTAAATAG CCTCCTTGAT GAAAACCGAA TTCAAGATCT GTCTCTCCTG TATCAGCTCT 
     2641 TCAGTAGAGT TCGGGGAGGA GTTCAGGTTC TCCTACAGCA CTGGATTGAG TATATCAAGG 
     2701 CTTTTGGAAG TACCATTGTT ATTAATCCTG AAAAAGATAA AACTATGGTT CAAGAATTAT 
     2761 TGGACTTTAA AGATAAAGTT GACCATATAA TTGATACCTG CTTTCTGAAA AATGAAAAAT 
     2821 TTATCAATGC CATGAAAGAA GCATTTGAAA CCTTCATTAA TAAAAGACCA AATAAGCCAG 
     2881 CTGAACTGAT AGCTAAGTAT GTGGATTCAA AACTTCGTGC AGGCAACAAA GAAGCTACAG 
     2941 ATGAAGAGCT TGAGAAAATG TTGGATAAAA TAATGATTAT ATTTAGATTT ATCTATGGCA 
     3001 AAGATGTTTT TGAGGCCTTC TATAAGAAAG ACTTAGCCAA ACGCCTATTA GTTGGCAAGA 
     3061 GTGCATCAGT GGATGCTGAA AAATCAATGC TGTCCAAACT GAAACACGAA TGTGGAGCCG 
     3121 CTTTTACCAG CAAGCTTGAA GGCATGTTTA AAGACATGGA GCTTTCTAAA GATATCATGA 
     3181 TCCAGTTCAA ACAGTATATG CAGAACCAGA ATGTACCTGG CAATATTGAA TTAACTGTTA 
     3241 ATATCCTGAC AATGGGTTAT TGGCCAACAT ATGTGCCTAT GGAAGTCCAT TTGCCACCAG 
     3301 AGATGGTGAA GCTTCAGGAA ATTTTCAAGA CATTTTACTT AGGCAAGCAC AGTGGCAGGA 
     3361 AACTTCAATG GCAATCAACC TTGGGACACT GTGTGCTAAA AGCTGAATTT AAAGAGGGCA 
     3421 AAAAAGAACT TCAGGTCTCT CTTTTTCAAA CCCTGGTGCT ACTAATGTTT AATGAAGGAG 
     3481 AGGAGTTCAG TTTAGAGGAG ATAAAGCAAG CCACTGGAAT AGAGGATGGA GAATTAAGAA 
     3541 GAACACTGCA GTCACTAGCC TGTGGCAAAG CTAGAGTTCT GGCTAAAAAT CCAAAGGGCA 
     3601 AAGATATTGA AGATGGTGAC AAGTTTATTT GTAATGATGA TTTCAAACAC AAACTATTCA 
     3661 GGATAAAGAT CAATCAAATC CAGATGAAAG AAACGGTTGA AGAACAAGCA AGCACTACAG 
     3721 AAAGAGTATT TCAAGATAGA CAGTACCAAA TTGATGCTGC GATCGTCCGA ATTATGAAGA 
     3781 TGAGAAAAAC ACTTAGCCAC AACCTTCTTG TTTCAGAAGT CTACAATCAG CTAAAATTTC 
     3841 CAGTAAAACC TGCTGATCTT AAGAAGAGAA TAGAATCCCT AATTGACCGG GACTACATGG 
     3901 AAAGAGATAA AGAAAACCCA AATCAGTACA ACTATATTGC AACGCGTACG CGGCCGCTCG 
     3961 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4021 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4081 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4141 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4201 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4261 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4321 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4381 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4441 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4501 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4561 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     4621 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     4681 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     4741 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     4801 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     4861 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     4921 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     4981 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5041 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5101 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5161 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5221 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5281 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5341 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     5401 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     5461 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     5521 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     5581 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     5641 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     5701 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     5761 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     5821 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     5881 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     5941 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6001 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6061 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6121 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6181 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6241 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6301 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6361 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     6421 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     6481 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     6541 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     6601 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     6661 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     6721 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     6781 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     6841 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     6901 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     6961 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7021 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7081 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7141 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7201 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7261 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7321 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7381 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     7441 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     7501 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     7561 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     7621 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     7681 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     7741 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//