LOCUS       RR209230                8730 bp    DNA     circular  SYN 
DEFINITION  Pex1 (Myc-DDK-tagged ORF) - Rat peroxisome biogenesis factor 1 (Pex1), (10 ug)
ACCESSION   NM_001109220
VERSION     NM_001109220.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4887..4978 
                     /label="Myc-DDK"  
     misc_feature    5008..5593 
                     /label="PolyA signal"  
     misc_feature    5065..5084 
                     /label="Restriction Enzyme"  
     misc_feature    5869..6541 
                     /label="ColE1"  
     misc_feature    6971..7765 
                     /label="Kan/Neo"  
     misc_feature    7800..8102 
                     /label="SV40 ori"  
     misc_feature    8188..8665 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4869..4875 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4869 
                     /label="Pex1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTGGAGCAGT GATCGCTTAG CCGGTGCGGG GTCTGGTGGG GCGGTCGTGA 
     1081 CGGTGGCCTT CACGAATGCT CGGGACTGCT TCCTCCATCT GCCACGGCAC CTCGTGGCCC 
     1141 AGCTTCACCT GCTGCAGAAT CAAGCTATAG AAGTAACCGG GGATCACCAG CCTACCTACT 
     1201 TGAGCTGGGT GGAAGGCAGG CATTTTAATG ATCAAAGTGA AAATGTGGCA GAAATTAATA 
     1261 GACAAGTTGG CCAGAAGCTT GGACTCTCGA GCGGGGATCA GGTGTTTCTC AGGCCGTGTT 
     1321 CCCATGTGGT CTCATGCCAG CAGGTTGAGG TGGAGCCTCT CTCGGCAGAC GACTGGGAGA 
     1381 TACTGGAGCT GCACGCCATT TCCCTTGAAG AACACCTTCT AGATCAGATT CGAATAGTTT 
     1441 TCCCTAAAGC CATCGTTCCC ATCTGGGTTG ACCAGCAGAC CTACATATTT ATCCAAATTG 
     1501 TCGCTCTGAT GCCAACTGCC CCTTATGGAA GGCTAGAAAC TAACACCAAA CTCCTTATTC 
     1561 AGCCAAAGAC CCGCCAAGCC AAAGAGAGCA CATTTCCAAA AGAAGGCGAT GCACGTGGAC 
     1621 AAGCTCATAA TTATGGGCGA GAACAGAAAG GAACGGTAAA GGAATTGCAA ACCAAGCAGC 
     1681 TTCATAAGAA CACAGAGGCT GTCCCTGTAT CTAATGGAAG AAACCCGGAA GTCCCAGGTG 
     1741 ACTCAGTGAA GCCACGCTGG TGGGCTGTGC TAGGAAGCAT GTTTTCCTTT GGGCCTGACA 
     1801 ACAAACAAGA GTCAGCTTGG GGCTCATTGG AACTCGGTGC TTTCAAAAAC ATGCAGTCAC 
     1861 AGACTGTCCC TCTAGACAGT ATTTTCAGAG TGTGCCAAGT TCAGCCTCCT AGTGTGCATA 
     1921 ACACCCCTGC TAACTCCGTG TTTCACAAAC ACTGCACCGT CCATGTATTT CCATGGGACC 
     1981 AAGAATATTT TGATGTGGAG CCCAGCTTTA CTGTGGTCTA TGGGAAACTA GTTAAGCTAC 
     2041 ATTCTCCAAA ACAACAGCAG GACAAAAGTA AGCAGGGCGT CCTGTTACCT GAAAAAGAGA 
     2101 AGCAACTGTC CCAGTCTCCA GGTCATAAGC GGATCGGCTC CGATGGCAGT GAGGAAGCTG 
     2161 CTGAGACCTG TGTGCTGAAG GTAGTCTGGA ATGGACTGGA GGAGCTGAAG AATGCCACGG 
     2221 AGTTCACCAA AAGTCTAGAG CCTCTCCACC ATGGGAAAGT CTGGATTCCA GCTGACCTGA 
     2281 GAAAGAGACT CAACATAGAA ATGCATGCAG TAGTCAGGAT AACTCCACTG GAAACCACCC 
     2341 CTGTCATCCC AAGAGCCCTG AGATTACAAC CTGCAGAGAA CTTACCTAGA GACGTGAGTG 
     2401 AAGAAGAGGT AAAAGCTGTG TTCTCATCGT GGCTACAGGA GTCTGCCACC ACCATGTTTC 
     2461 CTTTGATAAT ATCAAAGCAA GAATGTATTA AGCTGGAAAT TAAAGATGGG TTGAAAGAGT 
     2521 TCTCTCTGAG CATAGTTCAT TCTCAGGAAA AAGAGAAGGA ACAAGGAAGA ACTGTTTTTG 
     2581 TGTTGAGTCC CATTCTGCTG CAGAAGATCT CAGTACAAGT CCTTCTAGAG CCCATGATCG 
     2641 GAGAACACAG TGAGGAAATT GACATTCTTC TTCCCTCTTT AACACTGAGC TCTTTGGGCG 
     2701 GTGTGAGTTC CTTAAGTGTA TCTGCCATGG AACACATCAC TCACAGTCTC CTGGGACGCC 
     2761 CTTTGTCTCG GCAGCTGATG GCCCTCGTTG CCGGACTGAG GAATGGGGCT CTTTTGATCA 
     2821 CTGGAGGAAA GGGAAGCGGG AAATCAACGT TAGCTAAAGC CATCTGTAAG GAAGCACATG 
     2881 ACGCTCTGGA TGCCCACGTG GAGATGGTCG ACTGCAAAGC TTTACGAGGA AAAAGGCTTG 
     2941 AAAGCATACA AAAAGCCCTA GAGGTGGCTT TCTCAGAGGC AGCCTGGAGG CAGCCATCCG 
     3001 TCATTCTGCT GGATGACCTG GACCTCATTG CTGGATTACC GAGCACCCCA GAGCATGAGC 
     3061 ACAGCCCTGA AGCAGTGCAG AGCCAACGGC TTGCACACGC TTTGAACAAT ATGATCAAAG 
     3121 AGTTTGTTTC CATGGGAAGC TTGGTCGCGC TCATCGCCAC GAGCCAGCTC CAGCACTCTC 
     3181 TCCATCCTTC CCTTGTCTCT GCTCAAGGAG TCCACACATT TCAGTGCATC CAGCACATCC 
     3241 AGCCTCCTGA TCAGGAACAG AGATGTGAAA TTCTGCATAG TGTTGTGAAG AATAAACTGG 
     3301 GCTGTGATAT AAGCAAGTCC CCTGACTTGG ACCTGAAGTG CATAGCTAAA GAGACGGAGG 
     3361 CGTTTGTGGC TCGAGACTTT ACAGTTCTCG TGGACCGAGC CATACACTCC TCTCTCTCTC 
     3421 GCCAGCAGAA CCCCACCAGG GAAGGATTGA CTTTAACAAC AGCAGACTTC CAAAAGGCTC 
     3481 TCCGTGGATT TCTTCCTGCT TCTCTGCGAA ATGTCAACTT GCATAAACCT AGAGACCTGG 
     3541 GCTGGGACAA GATCGGTGGA TTACATGAAG TTCGGCAGAT ACTCATGGAT ACTATCCAGT 
     3601 TACCAGCCAA GTACCCAGAA TTATTTGCAA ACTTACCCAT ACGACAGAGG ACGGGAATAC 
     3661 TGCTTTATGG CCCTCCTGGG ACAGGAAAAA CTTTACTCGC TGGGGTGGTT GCAAGAGAGA 
     3721 GTGGGATGAA CTTTATTAGT ATTCAGGGAC CAGAGTTACT CAGCAAATAC ATTGGAGCAA 
     3781 GTGAGCAAGC TGTTCGAGAT GTCTTCATCA GAGCACAGGC TGCAAAGCCC TGTATTCTTT 
     3841 TCTTTGATGA GTTTGAATCC ATTGCTCCTC GAAGAGGTCA TGACAACACA GGGGTTACAG 
     3901 ACCGAGTAGT TAACCAGTTG CTGACACAGT TAGATGGAGT AGAAGGCTTA CAGGGAGTTT 
     3961 ATGTGTTGGC TGCTACTAGT CGCCCTGATT TGATTGACCC CGCCTTGTTA CGGCCCGGCC 
     4021 GACTGGATAA ATGTGTATAC TGCCCTCCTC CAGATCAGGT GTCTCGTCTT GAGATTTTAA 
     4081 CTGTCCTCAG CAAGTCTCTA CCTCTGGCGG ATGACGTGGA CCTTCAGCAC GTGGCCTCTG 
     4141 TCACTGAGTC CTTCACTGGA GCGGATCTGA AAGCTCTGCT GTACAATGCA CAGCTGGAGG 
     4201 CCTTGCAGGG ACGGCTACTG CCCGGCGGGC TCCACGATGG AGGATCCAGC TCTGACAGTG 
     4261 ACCTCAGTCT GTCTTCAATG GTCTTTCTTA ACCACAGCAG TGGTTCCGAC GACTCAGCTG 
     4321 GAGATGGAGA ATGTGGCTTA GATCAATCCC TCGTTTCTCT CGAGATGTCG GAGATCCTTC 
     4381 CAGATGAATC AAAATTCAAT ATGTACCGGC TGTACTTTGG CAGCTCCTAT GAATCGGAAC 
     4441 TTGGAAACGG AGCCTCTTGT GACTTGAGCT CACACTGTCT GTCTGCACCG AGCTCCATGA 
     4501 CTCAGGACTT ACCTGCCACT CCTGGGAAAG ACCCATTGTT TACACAGCAT CCTGTGTTCA 
     4561 GGACACCTTC CCAAGAAGGC TCCCAAGACC TTACACAGGA GCAGAGAGAC CAGCTGAGAG 
     4621 CAGACATCAG CATCATCAAA GGCAGATACC AGAGCCAAAG TGGAGAGGAT GAATCCCTTA 
     4681 ACCAGCCTGG ACCAATCAAA ACCAGTTTTG CTATTAGTCA GGCACATTTA ATGACTGCGC 
     4741 TTGCCCACAC GAGACCGTCC ATTAATGAGG ATGAAGGCAA GGAATTTGCT GAGCTGTATG 
     4801 AAAACTTTCA AAATCCGAAG AAGAGAAAAA ATCAAAGTGG AACAGTGTTT CGAACTGGAC 
     4861 AGAAAGTAAC GTTAGCAACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4921 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     4981 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     5041 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     5101 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     5161 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     5221 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     5281 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     5341 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5401 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5461 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5521 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5581 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5641 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     5701 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5761 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5821 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     5881 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     5941 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     6001 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     6061 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     6121 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     6181 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     6241 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     6301 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     6361 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6421 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6481 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6541 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6601 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     6661 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6721 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6781 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6841 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6901 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     6961 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     7021 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     7081 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     7141 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     7201 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     7261 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     7321 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     7381 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7441 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7501 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7561 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7621 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     7681 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7741 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7801 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7861 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7921 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     7981 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8041 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8101 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     8161 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     8221 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     8281 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     8341 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     8401 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     8461 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     8521 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8581 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     8641 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     8701 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//