LOCUS       RR209216                6955 bp    DNA     circular  SYN 
DEFINITION  Tp73 (Myc-DDK-tagged ORF) - Rat tumor protein p73 (Tp73), (10 ug)
ACCESSION   NM_001108696
VERSION     NM_001108696.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3112..3203 
                     /label="Myc-DDK"  
     misc_feature    3233..3818 
                     /label="PolyA signal"  
     misc_feature    3290..3309 
                     /label="Restriction Enzyme"  
     misc_feature    4094..4766 
                     /label="ColE1"  
     misc_feature    5196..5990 
                     /label="Kan/Neo"  
     misc_feature    6025..6327 
                     /label="SV40 ori"  
     misc_feature    6413..6890 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3094..3100 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3094 
                     /label="Tp73"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCATCACCT AGGCAACTGG ACGCTTATGC CACTGCCCTG CCACTGCAAG 
     1081 CCACAGGACC TCCAGCACCT CTGCCCAAGT CTACGGACCC ATCCAGGGCC CAGGTTCAGC 
     1141 CAAGAATGAG CAACGGCGTC GGGGAGATGG CCCAGTCCTC TTCCTCCTCC TCTTCCTCCT 
     1201 CCACCTTCGA GCACCTGTGG AGTTCTCTGG AGCCAGACAG TACCTACTTT GACCTTCCCC 
     1261 AGCCCAGCCA AGGGAATAGC GAGGCAACGG GCAGCGAGGA GTCCAACATG GATGTCTTCC 
     1321 ACCTGCAAGG CATGACCTCC TCCGTCATGG CCCAGTTCAA TTTGCTCAGC AGCGCCATGG 
     1381 ACCAGATGGG CAGCCGCGCG GCCCCGGCCA GCCCCTACAC CCCGGAGCAC GCCGCCAGCG 
     1441 CACCCACCCA CTCGCCCTAC GCGCAGCCCA GCTCCACCTT CGACACCATG TCTCCAGCGC 
     1501 CTGTCATCCC TTCCAACACC GACTACCCTG GTCCCCACCA CTTCGAGGTC ACCTTCCAGC 
     1561 AGTCGAGCAC AGCCAAGTCG GCCACCTGGA CATACTCCCC ACTTTTGAAG AAGTTGTACT 
     1621 GTCAGATTGC CAAGACATGC CCCATCCAGA TCAAAGTGTC CACACCACCA CCTCCGGGCA 
     1681 CGGCCATCCG GGCCATGCCC GTCTACAAGA AGGCAGAGCA TGTGACCGAC ATTGTTAAGC 
     1741 GCTGTCCCAA CCACGAGCTT GGAAGGGACT TCAATGAAGG ACAGTCTGCT CCGGCTAGTC 
     1801 ACCTCATCCG TGTGGAAGGC AACAACCTCT CCCAATATGT GGATGACCCT GTCACCGGCA 
     1861 GGCAGAGTGT GGTTGTGCCA TATGAACCCC CACAGGTAGG AACAGAATTC ACCACCATCT 
     1921 TGTACAACTT CATGTGTAAC AGCAGCTGCG TAGGGGGCAT GAATCGGAGA CCCATCCTCG 
     1981 TCATCATCAC CCTGGAGACC CGGGATGGAC AGGTACTGGG CCGGCGGTCT TTCGAGGGCC 
     2041 GCATCTGCGC CTGTCCTGGC CGTGACCGAA AGGCCGATGA AGACCATTAC CGGGAGCAAC 
     2101 AGGCTCTGAA TGAAAGCACC ACCAAGAACG GGGCTGCCAG CAAACGTGCG TTCAAGCAGA 
     2161 GCCCCCCTGC CATCCCTGCC CTGGGTACCA ACGTAAAGAA GAGACGCCAT GGTGACGAGG 
     2221 ACATGTTCTA CATGCATGTT CGAGGCCGGG AGAACTTTGA GATCTTGATG AAAGTCAAGG 
     2281 AGAGCCTAGA GCTGATGGAG CTTGTGCCGC AGCCTTTGGT TGACTCCTAC CGACAGCAAC 
     2341 AGCAGCAGCA GCTCCTACAG AGGCCGAGTC ACCTGCAGCC TCCCTCCTAT GGGCCCGTGC 
     2401 TGTCCCCAAT GAACAAGGTA CACGGTGGTG TCAACAAACT GCCCTCTGTC AACCAGCTGG 
     2461 TAGGCCAGCC TCCCCCACAC AGCTCTGCAG CTGGGCCCAA CCTGGGGTCC ATGGGTTCCG 
     2521 GGATACTCAA CAGCCACAGC CACAGCATGC CAGCCAATGG AGAGATGAAT GGAGGCCACA 
     2581 GCTCCCAGAC CATGGTTTCC GGATCCCACT GCACCCCGCC ACCCCCGTAC CATGCCGACC 
     2641 CCAGCCTCGT CAGTTTTTTG ACAGGGTTGG GGTGTCCGAA CTGTATCGAG TGCTTCACTT 
     2701 CCCAAGGGTT ACAGAGCATC TACCACCTGC AGAACCTCAC CATCGAGGAC CTTGGGGCCC 
     2761 TGAAGATCCC TGACCAGTAC CGTATGACCA TCTGGAGGGG CCTACAGGAC CTGAAGCAGA 
     2821 GCCATGACTG CGGTCAGCAG CTTCTACGCT CCAGCAGCAA CGCGGCCACC ATCTCCATCG 
     2881 GCGGCTCTGG CGAGCTGCAG CGGCAGCGGG TCATGGAAGC CGTGCATTTC CGCGTGCGCC 
     2941 ACACCATCAC GATCCCCAAC CGCGGAGGCG CGGGTGGCGT GACGGGCCCC GACGAGTGGG 
     3001 CAGACTTTGG CTTTGACCTG CCTGACTGCA AGTCCCGTAA GCAGCCCATC AAAGAGGAGT 
     3061 TCACAGAGAC AGAGAGCCAC GTTCACAGAG ACAGAGAGCC ACACGCGTAC GCGGCCGCTC 
     3121 GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT 
     3181 GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG 
     3241 GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT 
     3301 GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT 
     3361 CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC 
     3421 CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT 
     3481 CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT 
     3541 GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG 
     3601 TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG 
     3661 GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT 
     3721 TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC 
     3781 CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC 
     3841 TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA 
     3901 AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA 
     3961 AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC 
     4021 TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA 
     4081 CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC 
     4141 CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT 
     4201 CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT 
     4261 GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG 
     4321 AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA 
     4381 GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT 
     4441 ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA 
     4501 GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT 
     4561 GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA 
     4621 CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT 
     4681 CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA 
     4741 GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC 
     4801 CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT 
     4861 GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC 
     4921 GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG 
     4981 CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG 
     5041 CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA 
     5101 CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA 
     5161 GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC 
     5221 AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG 
     5281 GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT 
     5341 GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC 
     5401 ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC 
     5461 GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC 
     5521 TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT 
     5581 GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG 
     5641 CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC 
     5701 CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG 
     5761 CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG 
     5821 CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC 
     5881 TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC 
     5941 GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT 
     6001 GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA 
     6061 AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA 
     6121 TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG 
     6181 GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA 
     6241 TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT 
     6301 GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC 
     6361 ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG 
     6421 CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA 
     6481 ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG 
     6541 CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC 
     6601 CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC 
     6661 CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT 
     6721 CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC 
     6781 GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC 
     6841 TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT 
     6901 TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//