LOCUS       RR209171                8265 bp    DNA     circular  SYN 
DEFINITION  Nckap1 (Myc-DDK-tagged ORF) - Rat NCK-associated protein 1 (Nckap1), (10 ug)
ACCESSION   NM_031618
VERSION     NM_031618.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4422..4513 
                     /label="Myc-DDK"  
     misc_feature    4543..5128 
                     /label="PolyA signal"  
     misc_feature    4600..4619 
                     /label="Restriction Enzyme"  
     misc_feature    5404..6076 
                     /label="ColE1"  
     misc_feature    6506..7300 
                     /label="Kan/Neo"  
     misc_feature    7335..7637 
                     /label="SV40 ori"  
     misc_feature    7723..8200 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4404..4410 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4404 
                     /label="Nckap1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCGCGCTCC GTGCTGCAGC CCAGTCAGCA GAAGCTGGCG GAGAAGCTCA 
     1081 CCATCCTCAA CGACCGGGGC GTCGGCATGC TCACGCGCCT CTACAACATC AAGAAGGCAT 
     1141 GTGGAGATCC CAAGGCTAAA CCATCCTACC TTATTGACAA AAACCTGGAA TCTGCTGTGA 
     1201 AATTCATAGT CAGAAAATTT CCTGCTGTAG AAACACGCAA CAACAATCAA CAGCTTGCAC 
     1261 AACTACAGAA AGAAAAATCA GAGATTCTGA AAAATCTGGC ATTATATTAC TTTACATTTG 
     1321 TAGATGTTAT GGAATTTAAG GACCATGTCT GTGACTTGCT GAATACTATC GACGTCTGCC 
     1381 AAGTCTTCTT TGATATTACT GTGAACTTTG ATTTAACAAA GAACTACTTA GACTTGACTG 
     1441 TGACCTACAC AACATTAATG ATACTGCTGT CCCGGATTGA AGAAAGGAAG GCCATCATTG 
     1501 GACTGTACAA TTATGCACAT GAAATGACCC ACGGAGCAAG TGACCGAGAA TACCCACGCC 
     1561 TTGGTCAGAT GATTGTGGAC TATGAACACC CTTTAAAGAA GATGATGGAA GAATTCGTAC 
     1621 CCCACAGCAA GTCTCTTTCA GATGCGCTAA TTTCTCTTCA GATGGTGTAT CCTAGACGGA 
     1681 ATCTTTCAGC AGATCAGTGG AGGAATGCCC AGTTGTTGAG TCTCATCAGT GCACCCAGTA 
     1741 CAATGCTTAA TCCTGCACAG TCTGACACCA TGCCTTGTGA ATATCTCTCT TTGGATGCAA 
     1801 TGGAGAAATG GATTATCTTT GGCTTTATTC TGTGCCATGG GATGCTAAAT ACCGAGGCTA 
     1861 CAGCACTGAA CCTTTGGAAG CTAGCTCTTC AGAGTAGCTC CTGCCTCTCT CTCTTTCGGG 
     1921 ATGAAGTTTT CCACATTCAC AAAGCTGCAG AAGATTTATT TGTAAACATT CGGGGCTATA 
     1981 ATAAACGGAT TAACGACATA AGAGAATGTA AGGAGGCGGC TGTATCCCAC GCTGGCTCAA 
     2041 TGCACAGAGA GAGACGCAAG TTTTTACGGT CTGCACTGAA AGAACTGGCA ACTGTCCTCT 
     2101 CGGATCAGCC TGGCCTGCTT GGTCCCAAGG CACTTTTTGT TTTCATGGCA TTATCTTTTG 
     2161 CCCGTGATGA AATTATCTGG CTACTCCGTC ATGCAGACAA CATGCCAAAG AAGAGTGCAG 
     2221 ATGACTTTAT AGATAAGCAC ATTGCTGAGC TGATCTTTTA CATGGAGGAG CTCAGAGCAC 
     2281 ATGTCAGGAA GTACGGGCCT GTCATGCAGA GGTACTACGT GCAGTACCTC TCCGGCTTCG 
     2341 ATGCTGTTGT CCTTAACGAG CTTGTGCAGA ATCTCTCTGT TTGCCCTGAA GATGAATCAA 
     2401 TCATCATGTC CTCCTTTGTT AACACCATGA CTTCCCTAAG TGTGAAACAA GTTGAAGATG 
     2461 GGGAAGTGTT TGATTTCAGA GGAATGAGAT TAGATTGGTT TAGGTTACAG GCATACACTA 
     2521 GTGTCTCCAA AGCTTCACTT AGCCTTGCAG ATCACCGAGA ACTCGGAAAG ATGATGAACA 
     2581 CAATAATATT CCATACAAAG ATGGTCGATT CCTTGGTGGA AATGTTGGTG GAAACATCCG 
     2641 ATCTCTCCAT ATTTTGTTTT TATAGCCGTG CTTTTGAGAA GATGTTTCAG CAGTGCTTGG 
     2701 AGTTGCCTTC TCAGTCGCGA TACTCCATCG CCTTTCCTCT GCTCTGCACT CACTTCATGA 
     2761 GCTGCACACA TGAGCTGTGT CCCGAAGAGC GCCATCATAT AGGGGATCGT AGCCTTTCCC 
     2821 TGTGTAATAT GTTCCTCGAT GAGATGGCCA AACAAGCTCG GAACCTCATC ACTGATATTT 
     2881 GCACAGAACA GTGTACTCTT AGTGACCAGT TACTGCCGAA GCATTGTGCC AAAACCATCA 
     2941 GTCAAGCAGT GAATAAGAAG TCAAAAAAAC AGACTGGCAA GAAAGGGGAA CCTGAAAGGG 
     3001 AAAAGCCGGG TGTCGAGAGC ATGAGGAAAA ACAGGCTGGT AGTGACCAAC CTTGATAAGT 
     3061 TGCACACTGC ACTTTCGGAG TTATGCTTCT CCATAAATTA TGTTCCAAAC ATGGCAGTGT 
     3121 GGGAACACAC CTTCACACCG CGGGAGTACT TGACTTCTCA TCTGGAAATC CGATTCACTA 
     3181 AATCAATTGT TGGAATGACC ATGTATAATC AGGCTACACA GGAGATTGCA AAGCCATCAG 
     3241 AACTTCTGAC AAGCGTAAGA GCATATATGA CTGTACTCCA GTCCATAGAG AACTACGTGC 
     3301 AGATTGATAT CACCAGAGTA TTTAATAATG TTCTCCTTCA ACAAACACAA CACTTAGACA 
     3361 GCCATGGAGA ACCAACCATC ACAAGCCTGT ATACAAATTG GTACTTGGAA ACGTTATTAA 
     3421 GACAAGTCAG CAATGGCCAT ATAGCTTATT TTCCTGCAAT GAAAGCATTT GTGAATTTAC 
     3481 CCACAGAAAA TGAATTAACA TTCAATGCAG AGGAATACTC TGATATATCA GAAATGAGGT 
     3541 CGTTGTCAGA GCTCTTAGGC CCATATGGGA TGAAGTTCCT AAGCGAGAGT CTTATGTGGC 
     3601 ATATTTCATC ACAAGTTGCT GAACTTAAGA AACTTGTGGT GGAGAATGTT GATGTTCTAA 
     3661 CACAAATGAG GACCAGCTTT GACAAACCAG ACCAGATGGC TGCACTCTTT AAAAGATTAT 
     3721 CATCTGTTGA CAGTGTCTTG AAAAGGATGA CGATAATTGG TGTTATTTTA TCCTTCCGGT 
     3781 CACTGGCACA AGAAGCACTG AGAGATGTTT TGTCCTACCA CATTCCTTTT CTCGTGAGCT 
     3841 CCATTGAGGA TTTCAAGGAC CACATTCCAC GGGAAACCGA TATGAAGGTT GCAATGAATG 
     3901 TGTATGAGCT GTCATCTGCT GCTGGATTAC CATGCGAGAT TGATCCTGCC TTAGTAGTAG 
     3961 CTCTTTCCTC ACAAAAATCA GAGAACATTA GTCCAGAAGA AGAATATAAG ATCGCTTGCC 
     4021 TCCTGATGGT CTTTGTAGCA GTTTCCCTGC CTACACTGGC CAGCAATGTG ATGTCCCAGT 
     4081 ACAGCCCTGC AATAGAAGGA CACTGCAACA ATATTCATTG TCTGGCCAAA GCCATCAACC 
     4141 AAATTGCTGC TGCTTTGTTT ACAATTCACA AAGGAAGCAT TGAAGACAGA CTTAAAGAAT 
     4201 TCCTGGCGCT TGCGTCCTCC AGTCTACTGA AAATCGGCCA AGAGACAGAT AAAACTACAA 
     4261 CGAGAAATAG AGAATCTGTT TATTTGCTAC TGGATATGAT TGTACAAGAA TCACCGTTCC 
     4321 TGACAATGGA TCTCTTGGAA TCTTGTTTTC CTTATGTGTT GCTGAGAAAT GCATACCACG 
     4381 CTGTCTACAA ACAAAGTGTC ACATCCTCTG CAACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4441 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4501 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4561 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4621 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4681 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4741 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4801 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     4861 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     4921 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     4981 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5041 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5101 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5161 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5221 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5281 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5341 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5401 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5461 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5521 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     5581 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     5641 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     5701 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     5761 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     5821 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     5881 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     5941 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6001 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6061 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6121 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6181 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6241 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6301 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6361 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6421 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6481 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6541 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6601 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     6661 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     6721 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     6781 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     6841 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     6901 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     6961 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7021 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7081 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7141 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7201 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7261 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7321 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7381 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7441 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7501 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7561 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7621 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     7681 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7741 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     7801 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     7861 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     7921 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     7981 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8041 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8101 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8161 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8221 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
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