LOCUS       RR209166                8340 bp    DNA     circular  SYN 
DEFINITION  Jak1 (Myc-DDK-tagged ORF) - Rat Janus kinase 1 (Jak1), (10 ug)
ACCESSION   NM_053466
VERSION     NM_053466.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4497..4588 
                     /label="Myc-DDK"  
     misc_feature    4618..5203 
                     /label="PolyA signal"  
     misc_feature    4675..4694 
                     /label="Restriction Enzyme"  
     misc_feature    5479..6151 
                     /label="ColE1"  
     misc_feature    6581..7375 
                     /label="Kan/Neo"  
     misc_feature    7410..7712 
                     /label="SV40 ori"  
     misc_feature    7798..8275 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4479..4485 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4479 
                     /label="Jak1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAGTATCTA AATATAAAAG AGGACTGCAA TGCCATGGCT TTCTGTGCTA 
     1081 AAATGAGGAG CTTCAAGAAG ACGGAGGTGA AGCAGGTGGC CCCTGAGCCA GGAGTGGAAG 
     1141 TGACTTTCTA TCTGTTGGAC AGGGAGCCCC TCCGCCTGGG CAGCGGAGAG TACACCGCGG 
     1201 AGGAGCTGTG CATCAGGGCC GCACAGGAAT GCAGTATCTC TCCTCTCTGC CACAACCTCT 
     1261 TCGCCTTGTA TGATGAGAGC ACCAAGCTCT GGTATGCTCC AAACCACATC ATCACTGTGG 
     1321 ATGACAAAAT GTCTCTCCGG CTGCACTACC GCATGAGGTT CTACTTTACC AACTGGCATG 
     1381 GAACCAATGA CAATGAACAG TCTGTATGGC GACATTCTCC AAAGAAGCAG AAAAATGGCT 
     1441 ATGAGAAGAA AAAGGTTCCA GAAGCAACCC CACTCCTTGA TGCCAGTTCA CTGGAGTATC 
     1501 TGTTTGCGCA GGGACAGTAT GATTTGATCA AATGCCTGGC TCCCATTCGG GACCCCAAGA 
     1561 CAGAGCAAGA TGGACATGAT ATTGAGAATG AGTGCCTGGG CATGGCTGTC CTGGCCATCT 
     1621 CACACTATGC CATGATGAAG AAGATGCAGT TGCCAGAGCT TCCCAAAGAC ATCAGCTACA 
     1681 AGCGATATAT TCCAGAAACA TTGAATAAAT CCATCAGACA GAGGAATCTT CTTACCAGGA 
     1741 TGCGAATAAA TAATGTTTTC AAGGATTTCT TGAAGGAATT TAACAACAAG ACCATCTGTG 
     1801 ACAGCAGTGT GTCTACACAC GACCTGAAGG TGAAGTACCT GGCTACCTTG GAAACCTTAA 
     1861 CCAAGCATTA TGGAGCCGAG ATATTTGAGA CTTCTATGCT CCTGATTTCA TCAGAAAATG 
     1921 AGCTGAGTCG GTGCCATTCC AACGACAGTG GCAATGTTCT CTATGAAGTC ATGGTAACAG 
     1981 GAAATCTCGG GATCCAGTGG CGGCAGAAAC CAAATGTTCT TCCTGTTGAA AAGGAAAAAA 
     2041 ATAAACTGAA GCGGAAAAAA CTGGACTATA ATAAACACAA GAAGGAGGAT GACAGAAACA 
     2101 AACTCCGGGA AGAGTGGAAC AGTTTTTCCT ATTTCCCCGA GATTACCCAC ATTGTTATCA 
     2161 AGGAGTCTGT GGTCAGCATT AACAAACAGG ACAACAAGAA CATGGAACTA AAGCTCTCTT 
     2221 CTCATGAGGA AGCCTTGTCC TTCGTGTCCC TGGTAGATGG CTACTTCCGG CTCACTGCAG 
     2281 ATGCTCACCA TTACCTCTGT ACTGATGTGG CTCCCCCGCT GATTGTCCAC AACATACAGA 
     2341 ATGGCTGTCA CGGTCCAATC TGCACAGAAT ATGCCATCAA TAAGCTGCGG CAGGAGGGGA 
     2401 GTGAGGAGGG GATGTACGTG CTGAGGTGGA GCTGCACCGA CTTTGACAAC ATTCTTATGA 
     2461 CTGTCAGCTG CTTCGAAAAG TCCGAGGTAT TGGGTAATCA GAAGCAGTTC AAGAACTTTC 
     2521 AGATTGAGGT GCAGAAGGGC CGCTACAGCC TGCATGGCTC TGTTGACCAC TTTCCCAGCC 
     2581 TGAGAGACCT CATGAACCAC CTCAAGAAGC AGATCCTGCG CACGGACAAT ATAAGCTTTG 
     2641 TGCTGAAACG CTGCTGTCAG CCTAAGCCTC GAGAAATCTC CAACCTACTT GTAGCCACTA 
     2701 AGAAAGCCCA GGAGTGGCAG CCTGTCTACT CTATGAGCCA GCTGAGTTTC GATCGGATCC 
     2761 TTAAGAAAGA TATTATCCAA GGTGAGCACC TTGGCAGAGG CACAAGAACA CATATCTATT 
     2821 CTGGGACCCT GCTGGATTAC AAGGACGATG AAGGAATTGC TGAAGAAAAG AAGATAAAAG 
     2881 TGATTCTCAA AGTCCTAGAC CCTAGCCACC GGGACATTTC ACTGGCCTTC TTTGAGGCAG 
     2941 CCAGCATGAT GAGACAGGTT TCCCACAAAC ACATCGTGTA CCTCTACGGC GTCTGTGTCC 
     3001 GAGATGTGGA AAATATCATG GTGGAAGAGT TTGTGGAGGG GGGGCCATTG GATCTCTTCA 
     3061 TGCACCGGAA AAGTGATGCA CTTACTACCC CTTGGAAGTT TAAAGTTGCC AAACAGCTGG 
     3121 CCAGCGCCCT GAGTTACTTG GAAGATAAAG ACCTCGTTCA TGGAAATGTG TGCACTAAAA 
     3181 ACCTCCTCCT GGCCCGTGAG GGCATTGACA GTGACATTGG CCCGTTCATC AAGCTTAGTG 
     3241 ACCCTGGCAT CCCAGTCTCT GTGCTGACCA GGCAAGAGTG CATAGAGCGA ATTCCCTGGA 
     3301 TCGCTCCTGA GTGTGTTGAA GACTCCAAGA ACCTGAGTGT GGCTGCTGAC AAGTGGAGCT 
     3361 TTGGAACCAC ACTCTGGGAA ATCTGCTACA ATGGCGAGAT CCCACTCAAG GACAAGACCC 
     3421 TCATTGAGAA AGAGAGGTTT TATGAAAGCC GCTGCAGGCC AGTGACACCA TCTTGCAAGG 
     3481 AGCTAGCTGA CCTCATGACT CGCTGCATGA ACTATGATCC CAACCAGAGA CCTTTCTTCC 
     3541 GAGCCATCAT GAGGGACATT AACAAGCTGG AGGAGCAGAA TCCAGACATT GTTTCAGAAA 
     3601 AGCAGCCAAT AACAGAGGTG GATCCCACTC ATTTTGAAAA GCGTTTCCTA AAGAGGATTC 
     3661 GTGACTTGGG AGAGGGTCAC TTTGGGAAGG TTGAGCTCTG CAGATATGAT CCTGAGGGAG 
     3721 ACAACACAGG GGAACAGGTA GCTGTCAAGT CCCTGAAGCC TGAGAGTGGA GGTAACCACA 
     3781 TAGCTGATCT GAAGAAGGAG ATAGAGATCT TACGGAACCT CTACCACGAG AACATTGTGA 
     3841 AGTATAAAGG AATCTGCATG GAAGACGGAG GGAATGGTAT CAAGCTCATC ATGGAGTTTC 
     3901 TGCCTTCGGG AAGCCTAAAG GAATATCTGC CAAAGAATAA GAACAAAATC AACCTCAAAC 
     3961 AGCAGCTAAA ATATGCCATC CAGATTTGTA AGGGGATGGA CTATCTGGGC TCTCGGCAAT 
     4021 ATGTTCACCG GGACTTAGCA GCAAGAAATG TCCTTGTTGA GAGTGAACAC CAAGTGAAGA 
     4081 TCGGAGACTT TGGTTTAACC AAAGCAATTG AGACCGATAA GGAGTACTAC ACAGTCAAGG 
     4141 ATGACCGGGA CAGCCCAGTG TTTTGGTACG CTCCGGAGTG TTTAATCCAG TGTAAATTTT 
     4201 ATATCGCCTC TGATGTCTGG TCTTTTGGAG TGACACTGCA CGAGTTGCTC ACGTACTGTG 
     4261 ACTCAGATTT TAGTCCCATG GCCTTGTTCC TGAAAATGAT AGGCCCAACT CATGGCCAGA 
     4321 TGACAGTAAC ACGGCTTGTG AATACTCTGA AAGAAGGAAA GCGCCTGTCC TGTCCACCCA 
     4381 ACTGTCCCGA TGAGGTTTAT CAGCTTATGA GAAAATGCTG GGAATTCCAA CCATCTAATC 
     4441 GGACAACCTT TCAGAACCTT ATTGAAGGAT TTGAAGCACT TTTAAAAACG CGTACGCGGC 
     4501 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4561 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4621 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4681 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4741 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4801 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4861 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4921 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4981 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     5041 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     5101 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     5161 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5221 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     5281 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     5341 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     5401 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     5461 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5521 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5581 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5641 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     5701 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     5761 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     5821 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     5881 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     5941 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     6001 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     6061 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     6121 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     6181 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6241 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6301 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6361 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6421 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6481 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6541 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6601 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6661 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6721 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     6781 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     6841 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     6901 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     6961 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     7021 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     7081 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     7141 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     7201 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7261 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7321 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7381 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7441 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7501 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7561 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7621 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7681 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7741 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     7801 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     7861 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     7921 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     7981 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     8041 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     8101 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     8161 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     8221 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     8281 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//