LOCUS       RR209162                7791 bp    DNA     circular  SYN 
DEFINITION  Adcy1 (Myc-DDK-tagged ORF) - Rat adenylate cyclase 1 (brain) (Adcy1), (10 ug)
ACCESSION   NM_001107239
VERSION     NM_001107239.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3946..4037 
                     /label="Myc-DDK"  
     misc_feature    4067..4652 
                     /label="PolyA signal"  
     misc_feature    4124..4143 
                     /label="Restriction Enzyme"  
     misc_feature    4928..5600 
                     /label="ColE1"  
     misc_feature    6030..6824 
                     /label="Kan/Neo"  
     misc_feature    6859..7161 
                     /label="SV40 ori"  
     misc_feature    7247..7724 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3921..3928 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3921 
                     /label="Adcy1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCTCGGCG GTTGCCGAAC AGGGGGTTTG GCAACTCCTT TTGGTCACCT 
     1081 TCGTGTCCTA TGCCTTGCTG CCCGTGCGCA GCCTACTGGC CATCGGCTTC GGGCTCGTGG 
     1141 TGGCTGCCTC GCACTTACTG GTCACAGCCG CCTTGGTCCC TGCCAAGCGC CCACGTCTCT 
     1201 GGAGGACGCT TGGTGCCAAT GCCCTGCTCT TCTTTGGTGT GAACATGTAT GGCGTCTTCG 
     1261 TGCGGATCTT GACTGAGCGC TCCCAGAGGA AGGCCTTCCT GCAGGCTCGA AACTGCATTG 
     1321 AGGACCGGCT GCGGCTGGAA GATGAGAATG AGAAACAGGA GCGGTTGCTC ATGAGCCTCT 
     1381 TGCCCCGGAA TGTCGCCATG GAGATGAAGG AAGACTTCCT GAAGCCCCCT GAGAGGATAT 
     1441 TTCACAAGAT TTACATCCAG AGGCACGACA ATGTGAGCAT CCTGTTTGCA GACATCGTGG 
     1501 GTTTCACAGG TCTGGCGTCC CAGTGCACGG CCCAGGAGCT GGTGAAACTG CTCAATGAAC 
     1561 TCTTTGGCAA GTTCGATGAG TTAGCCACGG AAAACCACTG TCGCCGAATC AAGATCCTTG 
     1621 GAGACTGCTA CTACTGTGTG TCTGGCCTCA CCCAGCCCAA GACGGATCAC GCCCACTGCT 
     1681 GTGTGGAGAT GGGACTTGAC ATGATCGATA CCATCACATC CGTGGCTGAG GCTACTGAAG 
     1741 TAGACCTGAA CATGCGTGTG GGCCTACACA CTGGCAGGGT CCTCTGTGGT GTCCTGGGCC 
     1801 TACGCAAGTG GCAGTACGAC GTATGGTCCA ACGACGTCAC CCTGGCCAAT GTCATGGAGG 
     1861 CTGCTGGCTT ACCAGGGAAG GTCCACATCA CGAAGACAAC CCTTGCGTGC TTGAACGGGG 
     1921 ACTACGAGGT GGAGCCGGGC CACGGCCATG AGAGGAACAC CTTTCTGAGA ACTCACAACA 
     1981 TCGAGACCTT CTTTATTGTG CCATCCCATC GGAGGAAGAT ATTTCCAGGT TTGATTCTCT 
     2041 CGGATATCAA ACCAGCCAAG AGGATGAAGT TCAAGACCGT CTGCTACCTG CTGGTGCAGC 
     2101 TCATGCATTG CCGGAAAATG TTCAAGGCTG AGATCCCCTT CTCCAACGTA ATGACCTGTG 
     2161 AGGATGATGA CAAGCGAAGG GCATTGAGAA CGGCCTCGGA GAAGCTCAGA AACCGTTCGT 
     2221 CATTCTCTAC CAACGTGGTC TACACCACTC CGGGTACGCG TGTCAACAGG TACATCAGCC 
     2281 GCCTTCTTGA AGCCCGCCAG ACAGAGCTGG AGATGGCCGA CCTGAACTTC TTTACCCTCA 
     2341 AGTACAAGCA TGTTGAACGG GAACAAAAAT ACCACCAGCT TCAAGATGAG TACTTCACCA 
     2401 GCGCCGTTGT CCTCGCCCTC ATCCTGGCTA CCTTATTTGG GCTTATCTAC CTTCTGGTAA 
     2461 TCCCACAGAG CGTGGCTGTC CTGCTGCTGC TGGTGTTTTC TATCTGCTTC TTGGTGGCCT 
     2521 GTACCCTGTA CCTGCACATC ACGAGGGTCC AGTGTTTTCC AGGGTGCCTG ACCATCCAGA 
     2581 TCCGCACCGC TCTGTGTGTG TTCATCGTGG TCTTAATCTA CTCAGTAGCT CAAGGATGTG 
     2641 TGGTGAGCTG TCTGCCTTGG GCCTGGAGCT CTCACTCCAA CAGCTCCCTA GTGGTCCTCG 
     2701 CAGCTGGTGG CCGGCGCACT GTGCTGCCTG CCCTGCCCTG TGAGTCTGCA CACCATGGCC 
     2761 TGCTCTGCTG CCTGGTGGGC ACCCTCCCAC TAGCCATATT CCTGCGGGTG TCCTCCTTGC 
     2821 CAAAGATGAT CCTGCTGTCT GGGCTGACGA CTTCCTACAT CCTTGTCCTG GAGCTCAGTG 
     2881 GATACACCAA GGTTGGGGGC GGTGCCCTCT CTGGGCGCAG CTATGAGCCC ATCATGGCTA 
     2941 TCCTGCTGTT CTCATGCACG CTGGCCCTGC ACGCCAGGCA GGTGGACGTC AGGCTACGGC 
     3001 TGGACTACCT CTGGGCAGCA CAGGCGGAGG AGGAACGGGA CGACATGGAG AGGGTAAAGC 
     3061 TGGACAACAA GAGGATCCTC TTCAACCTCC TGCCAGCGCA CGTCGCACAG CACTTCCTCA 
     3121 TGTCCAATCC CCGGAACATG GATCTCTATT ACCAGTCCTA CTCCCAGGTG GGCGTCATGT 
     3181 TCGCCTCCAT CCCCAACTTC AACGACTTCT ACATAGAACT GGATGGCAAC AACATGGGCG 
     3241 TTGAGTGTCT GCGGCTCCTC AATGAGATCA TTGCTGACTT TGATGAGCTC ATGGACAAGG 
     3301 ACTTCTACAA GGACCTGGAG AAGATCAAGA CCATTGGGAG TACCTATATG GCTGCCGTGG 
     3361 GGCTGGCGCC CACAGCAGGA ACCAGGGCTA AGAAGTCCAT CTCTTCCCAT CTGAGCACGC 
     3421 TGGCAGACTT TGCCATCGAC ATGTTTGATG TCCTGGATGA GATCAACTAC CAGTCCTACA 
     3481 ACGACTTCGT TCTCCGAGTC GGCATCAACG TTGGCCCCGT GGTCGCTGGA GTGATCGGTG 
     3541 CTCGCAGGCC CCAGTACGAC ATCTGGGGAA ACACAGTCAA TGTGGCCAGT CGGATGGATA 
     3601 GCACTGGGGT CCAGGGAAGA ATTCAGGTGA CTGAGGAAGT CCACCGACTG CTGAACAGGT 
     3661 GCTCATACCA GTTTGTGTGC AGAGGCAAAG TCAGCGTCAA GGGCAAGGGA GAGATGCTAA 
     3721 CGTACTTTCT AGAAGGCAGG ACTGATGGGA GCAGTTCCCA CAGCAGGTCC CTTCGCTTGG 
     3781 AGCGAAGGAT GTTTCCTTAT GGGAGAGGCG GCGGAGGCCA GGCCAGGCGG CCCCCATTGT 
     3841 GTCCTGCAGC TGGACCCCCA ATCAAACCGG GGCTCTCTCC AGCCCCCACT AGCCAGTACC 
     3901 TGTCATCCAC AGCAGCAGGG AAGGAAGCCA GCGGACCGAC GCGTACGCGG CCGCTCGAGC 
     3961 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4021 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4081 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4141 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4201 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4261 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4321 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4381 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     4441 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     4501 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     4561 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     4621 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     4681 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     4741 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     4801 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     4861 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     4921 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     4981 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5041 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5101 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5161 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5221 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     5281 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     5341 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     5401 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     5461 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     5521 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     5581 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     5641 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     5701 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     5761 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     5821 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     5881 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     5941 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6001 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6061 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6121 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6181 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6241 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     6301 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     6361 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     6421 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     6481 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     6541 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     6601 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     6661 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     6721 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     6781 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     6841 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     6901 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     6961 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7021 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7081 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7141 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7201 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7261 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     7321 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     7381 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     7441 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     7501 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     7561 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     7621 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     7681 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     7741 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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