LOCUS       RR209155                7944 bp    DNA     circular  SYN 
DEFINITION  Tmem132a (Myc-DDK-tagged ORF) - Rat transmembrane protein 132A (Tmem132a), (10 ug)
ACCESSION   NM_178021
VERSION     NM_178021.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4101..4192 
                     /label="Myc-DDK"  
     misc_feature    4222..4807 
                     /label="PolyA signal"  
     misc_feature    4279..4298 
                     /label="Restriction Enzyme"  
     misc_feature    5083..5755 
                     /label="ColE1"  
     misc_feature    6185..6979 
                     /label="Kan/Neo"  
     misc_feature    7014..7316 
                     /label="SV40 ori"  
     misc_feature    7402..7879 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4083..4089 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4083 
                     /label="Tmem132a"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACGGAACGT GCGGCAGCGG CCCCTCGGGG GCCCTACGGT GCCTGGCTCT 
     1081 GCCTCCTGGT GGCTCTCGCC CTGGAAGTCG TGAGAGTTGG CAGTAACCAA AATACCCTGG 
     1141 ACCCCATCTA CCTGCCAGTG GCCCTGGAAC TCCTGGATGC CCCTGAACAT TTTCGGGTGC 
     1201 AGCAGGTGGG CCACTACCCA CCTGCCAACT CTTCTCTGGG CTCCCGCTCT GAGACCTTCC 
     1261 TGCTCATGCA GCCCTGGCCC AGGGCACAGC CACTTCTCCG GGCCTCCTAC CCACCTTTTG 
     1321 CCACTCAGCA GGTGGTCCCT CCTAGGGTCA CTGAGCCCCA CCGGCGACCT GTCCCTTGGG 
     1381 ATGTGCGGGC TGTTTCCGTG GAAGCAGCGG TGACTCCAGC AGAGCCCTAT GCCCGTGTCC 
     1441 TCTTCCACCT CAAAGGGCAG GATTGGCCAC CAGGACCTGG CAGCTTGCCC TGTGCCCGAC 
     1501 TACATGCCAC ACACCCTGCA GGCACTGCTC ACCAAGCCTG CCGCTTCCAG CCATCTCTGG 
     1561 GTGCCTGTGT AGTGGAACTG CAGTTCCCCT CTCAATGGTT CTCCCAAAGT GCCACCACAA 
     1621 GGGCAGAACT GGCCTACACT CTGGAGCCTG CAGGTGAGGG CCCTGGGGGC TGTGGCCCTG 
     1681 GCACGGAGGA GGAACCCAAG GAGCAGGCTC TTCCAGTGGG TAGTGTGGAG CTGCACCCTG 
     1741 AAGACCCCCC ACAGCACCAG GAGGTCCCGC TGGATGAAGT AGTCACTTTA CGTGCACCCG 
     1801 ATGTGCCAGT GAGGCCTGGC CAGCTCTTCA CTGCCACCCT TCTGCTTCGA CACAATTTCA 
     1861 CAGCCAGCCT TCTTACTCTG CGGATCAAGG TGAAGAAAGG GCTACAGGTG ACAGCTGCCC 
     1921 GCCCAGCCCA GCCCACCTTC TGGACCGCTA AGCTGGACCG CTTCAAGGGC TCAAAGCACC 
     1981 ACACCAGTCT GATCACCTGT CACCGCACTG GGCCTGCAGG GCCGGATTCC AGGCCCCTGG 
     2041 AACTGCCCGA GTTCCTGTGG GTAGACTTTG CGGTGGAGAA TAGCACCGGT GGGGGTGTGG 
     2101 CAGTCACTCG ACCTGTCACG TGGCAGCTGG AGTACCCAGG TCAGGCCCCT GAAGCAGAGA 
     2161 AGGACAAAAT GGTGTGGGAG ATCCTGTTTT CTGAGCGGGA CATCAGAGCC CTCATCCCTC 
     2221 TGGCCAAGGC TGAGGAGCTG GTGAACACGG CTCCGTTGAC TGGAGTGCCA CAGCGTATCC 
     2281 CTGTGCGTCT TGTCACTGTG GACAGTGGGG GAGCCCTGGA GGAGGTGACA GAGCACATTG 
     2341 GCTGTGAATC AGCCAATACC CAGGTCCTGC AGGTATCTGA GGCCTGTGAT GCTGTGTTCG 
     2401 TGGCTGGTCA GGAGAGTCGG GGAGCCAAGG GGGTTCGGGT GGACTTCTGG TGGCGCCGCC 
     2461 TCCGGGCCTC ACTGAAGCTG ACTGTATGGG CCCCACTCCT ACCCTTGCGC ATTGAGCTGA 
     2521 CTGACACCAC TCTTGAACAA ATCCGAGGCT GGCGGGTCCC AGGCCCAGCA GAAGGGCAGT 
     2581 TAGAGCCGGA GGCTGCTGCA GAGGAGGTGG AGCGGCGCTC CCGCGGCTGC CGCCTGCAGT 
     2641 ACCAGAGAGC TGGAGTACGC TTCTTAGTGC CCTTTGCGGC TCACCCGCTG GACGGTGGCC 
     2701 GCCGCCTCAC CCACCTACTC GGTCCTGACT GGTTGCTGGA TGTGAGTCAC CTCGTGGCAG 
     2761 CCCATGCCCA CGTGCAGGAT CCTCGCATAG CCACCTTGGA AGGTGGCCGC ATCCTAGTGG 
     2821 GACGAGAGCC TGGAGTCACC TCTATCGAGG TTCGATCCCC GCTATCTGAC TCCATCCTGG 
     2881 GGGAGCAGGC ACTGGCTGTG ACAGATGACA AGGTCTCGGT GCTGGATCTG CGAGTGCAAC 
     2941 CGGTGATGGG CATCTCACTG TCCCTGAGCC GGGGCGTGTC CCACCCTGGA GAGGTCACAG 
     3001 CGACTTGCTG GGCCCAGTCG GCTCTTCCAG CCCCAAAGCA GGAGGTGGCC CTCTCCCTGT 
     3061 GGCTGTCCTT TTCTGACCAC ACTTTGGCTC CAGCCGAGCT CTACGACCGC AATGACCTGG 
     3121 GACTGTCGGT CTCAGCTGAG GAACCCAGTG CTGTTGTGCC AGCTGAAGAG CAGAGAGCCC 
     3181 AACTTGGTGT GGTGGTGAGT GGCGTGGGTG CCAAAGGGCT GCCCCTACAC GTGGCTCTGC 
     3241 ACCCACCGGA ACCCTGCCGC CGGGGCCGCC ATCGTGTTCC CCTAGCCTCA GGCACAGCCT 
     3301 GGCTGGGGCT GCCCCCTTTG CCCACACCAG CTCCTGCACT CCCATCCAGC CCTGCCCGGA 
     3361 CCCCACCATT CACAGAAGCC TCTGTGGAAG GAAAGCGGCA GGTAGCAGGT GATATGGGGA 
     3421 GCCATGTGGG TCCGGGCATT AGGGGCAAGT TTGAGCGGGC AGAGGAGGAA GCTGGGAAGG 
     3481 AAGAGAATGA GGCCAAAGAA GAGGAGGAAG ATGAGGAAGA GATGGTGCCT GCCCCTCAGC 
     3541 GTGTCACCGA CCTAGAGCTG GGAATGTATG CTCTGCTGGG CATCTTCTGT TTGGCCTTCC 
     3601 TCATCTTCCT GGTCAATGGT GTGGTCTTTG TGTTGCGCTA CCAGCGCAAA GAGCCTCCCG 
     3661 ACAGCGTTAC AGACCCTGCC TCCCCTCAGC CACATAACTG GGTCTGGCTG GGCACCAATC 
     3721 AGGAGGAACT GAGTCGGCAG CTGGACCGAT GCTCTTCCTC GAGCCCACCC AAGGGGGAGG 
     3781 GGGGCTGCCC CTGTGAAAGT GGGGCAGGAG GGGATACTTC AACCGTGGCT CCCAGTGCTT 
     3841 CAGAGAGCCC TGCTGGCTCC ACGAGCACCC TGGCCCGCAA AGAAGCTGGG GGACGTCGGA 
     3901 AGCGAGTCGA ATTTGTGACA TTTGCACCAG CACCCCCAGC CCAGGAGGCG CCTGAGGAGC 
     3961 CTGTAGGGGC CCCGGCTGTA CAGTCCATCC TGGTAGCAGG TGAGGAGGAC ATCCGCTGGG 
     4021 TGTGCGAGGA CATGGGGCTG AAGGACCCAG AGGAGCTTCG GAACTACATG GAGAGGATCC 
     4081 GGGGCAGCTC CACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4141 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4201 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4261 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4321 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4381 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4441 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4501 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4561 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4621 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     4681 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     4741 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     4801 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     4861 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     4921 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     4981 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5041 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5101 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5161 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5221 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5281 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5341 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5401 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5461 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5521 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     5581 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     5641 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     5701 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     5761 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     5821 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     5881 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     5941 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6001 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6061 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6121 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6181 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6241 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6301 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6361 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6421 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6481 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6541 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     6601 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     6661 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     6721 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     6781 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     6841 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     6901 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     6961 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7021 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7081 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7141 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7201 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7261 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7321 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7381 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7441 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7501 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7561 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     7621 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     7681 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     7741 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     7801 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     7861 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     7921 TAACAAAAAT TTAACGCGAA TTTT
//