LOCUS       RR209139                9162 bp    DNA     circular  SYN 
DEFINITION  Col17a1 (Myc-DDK-tagged ORF) - Rat collagen, type XVII, alpha 1 (Col17a1), (10 ug)
ACCESSION   NM_001106366
VERSION     NM_001106366.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5319..5410 
                     /label="Myc-DDK"  
     misc_feature    5440..6025 
                     /label="PolyA signal"  
     misc_feature    5497..5516 
                     /label="Restriction Enzyme"  
     misc_feature    6301..6973 
                     /label="ColE1"  
     misc_feature    7403..8197 
                     /label="Kan/Neo"  
     misc_feature    8232..8534 
                     /label="SV40 ori"  
     misc_feature    8620..9097 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5301..5307 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5301 
                     /label="Col17a1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATGTGACC AAGAAAAGCA TGCGAGATGG CACTGAAGTC ACTGAGAGAA 
     1081 TTGTCACGGA AACAGTAACC ACAAGACTCA CATCCTTACC ACCAAAAGGG AGCACCAGCA 
     1141 ATGGATACAC TAAGACAGGC TCTCTGGGTG GAGGAAGCCG GCTAGAGAAA CAAAGTTTGA 
     1201 CCCATGGAAG CAGCGGCTAC ATCAACTCAA GCGGAAGCCT CCGAGGCAAC GCTTCCACCT 
     1261 CCAGTTACAG GAGAGCTCAC TCACCTGCCT CGACCCTGCC CAACTCTCCC GGCTCCACCT 
     1321 TTGAAAGGAA AACTCACATG AGCCGCCATG GAACTTACGA AGGGAGCTCC AGTGGCAACT 
     1381 CCTCACCTGA GTATCCACGG AAGGAACTCG CCTCTTCTTC AACCAGAGGA CGAAGCCAGA 
     1441 CACGAGAGAG CGAAATTCGA GTTCGACTAC AGAGTGCGTC GCCATCCACC AGATGGACAG 
     1501 AACTGGATGA AGTGAAGCGC TTGCTGAAGG GGAGCCGATC TGCCAGTGCC AGCCCCACCA 
     1561 GGAACACCTC CAACACACTC CCTATCCCCA AGAAAGGCAC CGTAGAAACC AAAACGGTGA 
     1621 CAGCGAGCTC CCACTCAGTG TCAGGAACCT ATGATTCAAC AATACTGGAC AGCAACTTTC 
     1681 CCCCCCACAT GTGGTCTTCC ACCTTGCCGG CAGGGTCCTC CATGGGGACC TATCACAACA 
     1741 ACATAGCGAC CCAGAGCACA TCCCTCGTCA ACACCAATGC CTACTCAGCA GGATCAGTCT 
     1801 TTGGAGTGCC AAATAACATG GCGTCCTGCT CTCCCACCCT GCACCCAGGA CTCAGCAGCT 
     1861 GCTCCTCAGT GTTTGGCATG CAGAACAATC TGGCCCCCTG CTCTTCTGTC CTCTCCCATG 
     1921 GCACAACCAC GGCTTCCACA GCATATGGTG TGAAGAAAAA CGTGCCCCAG CCACCCACTG 
     1981 TCACCAGCAC CGGCGTGTCC ACCTCTGCCG CCTGCACCAC CAGTGTCCAG AGCGACGACC 
     2041 TCTTGCATAA AGACTGCAAG TTCCTGATTC TGGAGAAAGA CAACGTGCCC GCCAAGAAGG 
     2101 AGATGGAGCT CTTGATCATG TCCAAGGACA GTGGGAAGGT CTTCACTGCC TCCCCCGCCA 
     2161 GCATCTCTTC AACTTCTTTT TCAGAGGACA CCCTGAAAAA GGAGAAGCAG GCTGCGTATG 
     2221 CCGCTGACAC ATGTCTAAAG GCAGATGTGA ACGGAGACCT AAACACTGTG TCTACGAAGA 
     2281 GCAAAGCCAT CTCGGCAGAA AACCACAGCT ACGACCGAGG TGTCGGTGGT GGCAGAGGCA 
     2341 AAAGCGGAGT CGCCGGCGGT GGTGGTGGTG GTGGCGGCGG CGGCAGCGGC GGCGGAACAT 
     2401 GGGGGGCTGC ACCTGCCTGG TGCCCCTGCG GCTCCTGCTG CAGCTGGTGG AAGTGGCTGC 
     2461 TGGGCCTGCT GCTCACTTGG CTGCTGCTGC TGGGTCTGCT CTTCGGCCTC ATTGCCCTGG 
     2521 CGGAGGAGGT AAGGAAGCTG AAGGCCCGCG TGGAGGAGCT GGAAAAGACC AAGGTGCTAT 
     2581 ATCATGACAT CGTCAAGACG GACCGAAGCA GCAGGGACCG CCTCCAGGGT GAGGCACCCA 
     2641 GCCTGGGACC TGGATTAAGC AAGGCTGAAA TGGACGACTA CGGCCAGGAG GCCATCTGGC 
     2701 TGTTTGTAAG GAACAAGCTG ATGACAGAGC AGGAGAACGG AAATCTCAGA GGAAGTCCTG 
     2761 GTCCAAAAGG TGACACGGGG AGTCAAGGAC CTAAAGACCG TGGCCTTCCT GGGATCCCAG 
     2821 GTACCCCTGG GCCCCTGGGC CACCCCGGCC CGGAAGGACC AAAGGGACAA AAAGGCAGCA 
     2881 TTGGAGATCC AGGCATGGAA GGACCCATAG GCCAGAGAGG ACTAGCAGGC CCCATGGGAC 
     2941 CTCCTGGTGA ACCTGGGCCT CCGGGGTCTG GAGAGAAAGG AGACAGAGGG ATTGCTGGAG 
     3001 AACAAGGTCC TCGGGGTCTT CCCGGTGTCC CGGGTTCTCC GGGTCTCAGA GGTACCAGTG 
     3061 GCTCTCCTGG TCCCCAGGGC CCCCCAGGCT CTGTGGGTCC CCAAGGGCTC CGAGGTGAAG 
     3121 TGGGACTTCC GGGTGTCAAA GGTGACAAAG GACTCGTGGG ACCACCAGGA CCCAAAGGTG 
     3181 ACCAGGGTGA GAAAGGACCC AGAGGCCTCA CAGGGGAGCC TGGCATCCGA GGTCTGCCTG 
     3241 GAGCTGTGGG TGAACCTGGA GCCAAAGGAG CAATGGGTCC CGCTGGCCCC GATGGACAGC 
     3301 AAGGTTCCAG AGGTGAACAA GGCCTGACTG GGATGCCTGG AGCCCGGGGC CCACCAGGAC 
     3361 CCACCGGAGA CCCAGGGAAG CCAGGTCTCA CAGGACCCCA AGGACCTCAG GGACTTCCTG 
     3421 GTACCCCTGG CCGACCAGGG ACTAAAGGTG AACCTGGCGC TCCAGGCAGA GTCATGACTT 
     3481 CAGAGGGATC CTCAGCAATC ACTGTGCCGG GACCTCCCGG ACCTCCCGGT GCCATGGGAC 
     3541 CCCCAGGACC TCCAGGGACT CCAGGTCCGG CTGGCCCTGC TGGTCTCCCA GGACAACAAG 
     3601 GCCCACGAGG GGAGCCGGGA CTTGCTGGGG ACTCCTTCCT AAACAGTGGC AGTTCCATCT 
     3661 CCGAAGTCCT CTCTGCCCAA GGTGTTGACT TTCGAGGTCC CCCTGGCCCA CCTGGCCCAC 
     3721 GAGGGCCACC AGGGCCTTCC ATTCCAGGCC CACCAGGACC CAGAGGCCCA CCAGGGGAAG 
     3781 GCGTACCAGG CCCACCCGGC CCACCAGGAT CCTTCCTGAC TGACTCGGAA ACCTTCTTCT 
     3841 CTGGCCCTCC AGGTCCACCT GGCCCCCCAG GTCCCAAGGG AGACCAAGGT GACCCCGGTG 
     3901 TCCCAGGCAC TCCTGGCATC CCTGGTGGTC TTTCTCATGG GACGTCGTCA GGCACTATGT 
     3961 ACGCACAGGG CCCACCTGGT CCCCCAGGAC CTCCTGGGCC TCCAGGATCT CTCAGCAGCT 
     4021 CCGGCCAGGA ACTCCAGCGC TATATTGCAG AGTACATGCA AAGTGACAAC ATCAGGACTT 
     4081 ATCTCTCAGG AGTTCAGGGT CCCCCCGGCC CACCAGGTCC CCCAGGGCCT GTCATCACCA 
     4141 TCACAGGGGA GACTTTCGAC TACTCCCAGC TGGCAAGCCA GGTTGTGAGT TACTTGCGAT 
     4201 CATCAGGCTA TGGTGTTGGC TTGTCCTCTG CCTCCTCCTC AGAAGATATC TTGGCCATGC 
     4261 TGAGACGCAA TGATGTGTGG CAGTACCTAC GTCAGCACCT GGTGGGCCCT CCGGGTCCCC 
     4321 CGGGACCACC AGGAGTCAGT GGAGATGGGT CCCTCCTGTC TTTGGACTAT GGAGAGCTAA 
     4381 GCAGACACAT TCTCAACTAT ATGTCAAGTT CTGGGATCAG CTTTGGGCAT CCTGGACCAC 
     4441 CGGGCCCCCC TGGCCTGCCA GGAACATCCT ATGAGGAGCT ACTGACCATG CTCCGAGCTG 
     4501 CTGGTCTGTC CTCCATCGCA GGCCCCCCGG GCCCCCCAGG TCCCCCAGGT CCTCGAGGGC 
     4561 CCCCGGGTGT GTCAGCAGCT CTGGCCACCT ACGCAGCTGA GAACAGTGAC AACTTCCGCA 
     4621 GTGAGCTGAT TAGCTACCTT ACAAGTCCTG ATGTCCGCAG CTTCATCGTT GGCCCTCCAG 
     4681 GTCCTCCAGG GCCACAGGGA CCACCTGGAG ACTACAACTG GGGTAGCGGC TCCTCAGCCA 
     4741 GAAGGGGCAC TTCCTACAGC TCTTCCATGG GCACAGGGAG AACCAATGGT GGCTCCCTGG 
     4801 GCGAAGGTGG AGCCTTTGGT GCAGGAGATG GGGGCCCCTA CGGCACTGAC GTCGGCCCAG 
     4861 GCGGAGGCTA CGGGGCAGCT GCAGGTGGTG GTGTATATGG CACCAATGGC GAATCATTCC 
     4921 GGGGCAGCTT CACTGGAGAC CTAGATTATA ACACGCTGGC ATTGCGGGTG TCGGAGAGCA 
     4981 TGCAGCGTCA GGGTCTGCTA CAAGGGATGG CCTACACTGT CCAGGGCCCA CCAGGGCCAC 
     5041 AAGGCCCTCC TGGCATCAGC AAGGTCTTCT CTGCCTACAG CAACGTGACA CAAGACCTCA 
     5101 TGGACTTCTT CCGAACATAT GGCGCTATCC CAGGACCCCC TGGGCAGAAA GGAGACATGG 
     5161 GAACCCCAGG TCCCAAAGGT GACAGGGGCC CTGCTGGACC ACGAGGTCCT CCGGGGCCCC 
     5221 CGGGCCCCCG AGGGAACAAA GGAGAGAAAG GGGATAAAGG TGACCAAGTC TATACTGGGA 
     5281 GGAGGAAGAG AAGTATTGCC ATCAAGCCAA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     5341 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     5401 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     5461 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     5521 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     5581 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     5641 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     5701 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     5761 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     5821 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     5881 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     5941 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     6001 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     6061 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     6121 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     6181 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     6241 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     6301 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     6361 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     6421 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     6481 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     6541 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     6601 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     6661 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     6721 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     6781 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     6841 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     6901 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     6961 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     7021 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     7081 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     7141 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     7201 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     7261 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     7321 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     7381 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     7441 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     7501 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     7561 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     7621 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     7681 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     7741 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     7801 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     7861 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     7921 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     7981 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     8041 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     8101 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     8161 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     8221 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     8281 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     8341 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     8401 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8461 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8521 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     8581 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     8641 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     8701 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     8761 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     8821 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     8881 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     8941 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     9001 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     9061 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     9121 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//