LOCUS       RR209119                8271 bp    DNA     circular  SYN 
DEFINITION  Inpp5f (Myc-DDK-tagged ORF) - Rat inositol polyphosphate-5-phosphatase F (Inpp5f), (10 ug)
ACCESSION   NM_001107554
VERSION     NM_001107554.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4428..4519 
                     /label="Myc-DDK"  
     misc_feature    4549..5134 
                     /label="PolyA signal"  
     misc_feature    4606..4625 
                     /label="Restriction Enzyme"  
     misc_feature    5410..6082 
                     /label="ColE1"  
     misc_feature    6512..7306 
                     /label="Kan/Neo"  
     misc_feature    7341..7643 
                     /label="SV40 ori"  
     misc_feature    7729..8206 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4410..4416 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4410 
                     /label="Inpp5f"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCTCTTT CAGGCCAAGG ACCACTACAT CCTGCAGCAG GGCGAGCGTG 
     1081 CCCTGTGGTG CAGCCGGCGC GACGGCGGCC TGCAGCTCCG ACCGGCCACT GACCTACTTC 
     1141 TTGCCTGGAA CCCCATTTGT TTGGGATTGG TAGAAGGAGT AATTGGGAAG ATTCAACTCC 
     1201 ATTCAGATCT TCCGTGGTGG CTTATTTTAA TTCGGCAGAA AGCATTGGTG GGCAAACTCC 
     1261 CAGGAGACCA TGAGGTCTGT AAAGTCACCA AGATTGCTGT ATTGTCACTT TCTGAAATGG 
     1321 AACCTCAGGA GCTTGAGCTG GAGCTCTGCA AGAAGCATCA TTTTGGAATT AACAAGCCTG 
     1381 AGAAGATTGT ACCATCTCCT GATGACTCCA AGTTTCTACT GAAGACATTC ACAAATATCA 
     1441 AATCCAATGT GTCTGCTCCC AACAAAAAGA AAGTTAAAGA AAGTAAAGAA AAGGAGAAAC 
     1501 TGGAGAGGCG TTTGCTTGAG GAGTTGCTGA AGATGTTCAT GGACTCAGAG TCCTTCTATT 
     1561 ATAGCTTGAC CTATGACCTA ACCAACTCAG TGCAGAGGCA GAGCACTGGG GAGAAGGATG 
     1621 GCCGCCCCCT GTGGCAGAAG GTTGATGATC GATTTTTCTG GAATAAGTAC ATGATCCAAG 
     1681 ACCTCTCTGA GATTGGTACT CCGGACGTGG ACTTCTGGAT CATCCCCATT ATCCAGGGTT 
     1741 TTGTGCAGAT TGAAGAACTG GTGGTTAATT ACGAGCCATC TGATGATGAC AAGAGCAGCC 
     1801 CAGAGACGCC CCCTCAGGAG GCCACTTGTG TAGATGACAT TCACCCGCGA TTTCTAGTGG 
     1861 CTCTTATATC TCGCAGGAGT AGGCACAGAG CAGGAATGCG GTATAAACGA AGAGGAGTGG 
     1921 ATAAAAATGG AAATGTTGCA AATTATGTGG AGACTGAGCA GTTAATTCAT GTTCATAATC 
     1981 ATACTCTGTC GTTTATTCAA ACTCGAGGCT CCGTGCCCGT CTTTTGGAGC CAGGTTGGGT 
     2041 ATCGCTATAA TCCAAGACCT CGACTAGACA AAAGTGAGAA GGAGACAGTC GACTGTTTCT 
     2101 GTGCCCATTT TGAAGAACAA CTGAAAATTT ACAAAAAGCA GGTTATTGTT AACTTGGTGG 
     2161 ACCAGGCTGG AAGGGAGAAG ATCATTGGTG ATGCTTACCT GAAGCAGGTG CTGCTCTTCA 
     2221 ACAACCCCAG GCTCACCTAC GTCTCCTTTG ATTTCCACGA GCACTGCCGA GGAATGAAGT 
     2281 TTGAGAATGT TCAAACCCTA ACGGATGCCA TTCATGACAT CATCCTTGAT ATGAAGTGGT 
     2341 GTTGGGTTGA TCAAGCTGGT GTGATATGTA AGCAAGAAGG AATTTTTCGA GTTAATTGCA 
     2401 TGGACTGCCT GGATCGCACC AACGTGGTCC AAGCTGCCAT TGCACGAGTG GTCATGGAAC 
     2461 AGCAGCTTAA AAAACTAGGT GTGATGCCCC CTGAGCAGCC ACTACCTGTG AAATGTAATC 
     2521 GGACCTACCA GATAATGTGG GCTAACAACG GCGACTCCAT CAGCAGACAG TATGCTGGCA 
     2581 CAGCTGCTCT AAAGGGCGAC TTTACAAGAA CAGGAGAAAG GAAGTTGGCA GGAGTTATGA 
     2641 AAGATGGAGT TAACTCAGCA AATAGGTACT ACCTCAGCCG GTTCAAGGAT GCTTACAGGC 
     2701 AAGCTGTCAT AGATTTGATG CAAGGCATTC CAGTGACGGA AGATCTTTAT TCCATATTTA 
     2761 CCAAGGAGAA GGAGCATGAA GCTTTGCAGA AGGAGACCCA GAGAAGCCAC CAAGAGCTCA 
     2821 TCAGCCAGCT CTTACAGAGC TACATGCAGT TGCTGCTGCC GGGGGATGAG AAGTTCCACG 
     2881 GGGGCTGGGC CCTCATTGAC TGTGACCCCA GCCTCACTGA TGCTGCTCAC AGAGATGTGG 
     2941 ACGTGCTACT GCTGCTTTCT AATTCTGCCT ACTATGTGGC CTATTATGAT GATGAAGTTG 
     3001 ATAAAGTAAG CCAGTATCAA CGACGGAGCC TTGAAGACCT GGAAAAGATA GAAATAGGTC 
     3061 CCGAGCCTAC TCTTTTTGGC AAACCAAAGT TCTCCTGCAT GCGGCTGCAC TACACGTGTA 
     3121 AAGAAGCAGG TGGTTACTTC CACACACTGA GAGCCGTACC ACGCAGTCCT GAAGAAGACG 
     3181 GGAAAGATAC TCTCCAGTGC ATTGCGGAGA TGCTGCAGAT CACCAAGCAG GCCATGGGGC 
     3241 TGCATGTGCC CATAATCGAG AGGAAACTCG AGAGGAAGAG CAGCAAGCCT CATGAAGACA 
     3301 TCATTGGTAT CAGATCTCAA AACCAAGGCT CTCTGGCCCA GGGGAAAAGC TTTTTAATGA 
     3361 GCAAATTTTC ATCTCTAAAT CAAAAAGTAA AGCAGACCAA ATCCAATGTG AACATTGGCA 
     3421 ACCTACGCAA ATTAGGAAAC TTTACAAAGC CTGAAGTGAA AGTGAACTTT CTAAAACCAA 
     3481 ACTTAAAAGT CAACCTTTGG AAATCAGATA GTAGTCTTGA GACCATGGAG AACCCAGGAG 
     3541 TGATGGGTAA CAAGGTCCAG GGGGAGTCTG ATGGGGACGT GTCCTCAGAT AATGACTCTT 
     3601 ACCACTCTGA TGAACTCCTT ACAAACTCCA GGTCTGAGGA AGGCAAGCAG CTAGCCAACT 
     3661 CTTTGGAGAG CGCAGGGCCA ACAGACTACC TTCTCCCGAG CTGTGGCATC ATCGCCTCAG 
     3721 CGCCTCGGTT AGGCAGCCGG TCCCAGTCGG CCAGCAGCAC CGACGTTAGC ATCCATGCTC 
     3781 CCTCGGAAGC TACTGTTGGT CATGGACATG AGCTCGGAAA AGGCCTGGAG TCTCCCCTGA 
     3841 AGAAAAGCCC TTCTGCTGAC AACATACACA CATTGACTGG CTTTGCAAAG CCCATGGACG 
     3901 TTTACTGCCA GAGATTCGTG CAGGATGCAC AGAACAAAAT GAACGCGCTG TCGGAGGTCA 
     3961 GGGCTGTAGC TCAGAACAGC GAGGAGGGAA ATCACAAGAC TAACCCTGTT TCTAATGAAG 
     4021 AAACCCAGTC AGAATCAGTG GAACAGATGC CTTCTCGGCC CTCTCAGTTA AATGTCTCTT 
     4081 GTTCTGTGAC AGGCCCACAG TTGTTATCAG TTGAACCAGC ACATCCAGCG GTATCTCAGA 
     4141 AGACCCCCGG CTCCGGGTCC AGCCTGCTGG ACCTTGAGGC AGGGCTTTGC GTAACTCCTT 
     4201 CTTCAGAGAG CAGCAGCAGA GCAGTCTCTC CCTTTGCAAA GATCCGCAGC TCCATGGTCC 
     4261 AGGTTGCTAA TATTACCCAA GCTGGGCTAA CTCATGGGAT AAACTTGGCA GTTGCCAAAG 
     4321 TTCAGAAGAG TCCAGCAGAA CCCGAAGTGG TTAACGAAAT TCAACAAAAT GAACTTAAAA 
     4381 ATATGTTTAC ACAATGCCAG ACACGAATAA TTCAGATTAC GCGTACGCGG CCGCTCGAGC 
     4441 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4501 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4561 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4621 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4681 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4741 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4801 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4861 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     4921 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     4981 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5041 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5101 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5161 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5221 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5281 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5341 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5401 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5461 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5521 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5581 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5641 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5701 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     5761 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     5821 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     5881 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     5941 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6001 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6061 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6121 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6181 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6241 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6301 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6361 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6421 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6481 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6541 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6601 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6661 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6721 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     6781 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     6841 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     6901 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     6961 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7021 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7081 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7141 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7201 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7261 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7321 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7381 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7441 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7501 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7561 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7621 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7681 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7741 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     7801 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     7861 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     7921 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     7981 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8041 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8101 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8161 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8221 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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