LOCUS       RR209109                8766 bp    DNA     circular  SYN 
DEFINITION  Map3k6 (Myc-DDK-tagged ORF) - Rat mitogen-activated protein kinase kinase kinase 6 (Map3k6), (10 ug)
ACCESSION   NM_001107909
VERSION     NM_001107909.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4921..5012 
                     /label="Myc-DDK"  
     misc_feature    5042..5627 
                     /label="PolyA signal"  
     misc_feature    5099..5118 
                     /label="Restriction Enzyme"  
     misc_feature    5903..6575 
                     /label="ColE1"  
     misc_feature    7005..7799 
                     /label="Kan/Neo"  
     misc_feature    7834..8136 
                     /label="SV40 ori"  
     misc_feature    8222..8699 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4896..4903 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4896 
                     /label="Map3k6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGGGCCG TGTCCCGGGG CCGGGGTTCT AGAACGGGCG GGCAGCTGCT 
     1081 GGCAGGACCC TCTGGCGGAG GCGCTGAGCC GGGGCCGCTC GTCTCCTGCG GTCGCCGGTC 
     1141 GGGGCTGCGC GCGGAGCCGG CCTCTCAGTG TGGTCTACGT GTTGACCCGC GAGCCTGGAC 
     1201 CCGGGGTGGA GCCCGGGTCG GGAACCGAGG CAGAGCCGCT GCCGCTGCGA TGCCTGCGCG 
     1261 AGGCCTGTGC TCAGCTCCAG AGCACGCGGC CACCCCCGCA ACTGCGCAGC CTGCCCTTTG 
     1321 CGACGCTGGC GCTAGGCGAC ACCGCTGCGC TAGACTCCTT CTACAACGCG GACGTAGTGG 
     1381 TGCTGGAGGT GAGCAACTCC CTGGCACAGC CTTCCTTGTT CTACCACCTC GGCGTGCGTG 
     1441 AGAGCTTCAG CATGACCAAC AATGTGCTCC TCTGTTCCCA GGCAGAGCTC CCTGACCTGC 
     1501 AGGCCCTTCG TGAGGATGTT TTCCAGAAGA ACTCGGATTG CGTTGGCAGC TACACACTGA 
     1561 TTCCTTATGT TGTGACAGCC ACTGGCCGGG TCTTATGTGG CGATGCAGGC CTCCTGAGGG 
     1621 GCATAGCCGA TGGGCTAGTA CAGGCTGGGG TGGGCACTGA GGCCCTCCTC ACGCCCCTGG 
     1681 TGGGCCGGCT TGTCCGTCTC TTGGAGGCCA CACCCACAGA CTCTTGCGGC TACTTCCGGG 
     1741 AGACCATTCG TCAAGATATC CGGAAGGCAC GGGAGCGGTT CAGCGGGCAG CAGCTGAGGC 
     1801 AGGAGCTGGC TCGCCTGCAG CGGAGGCTGG ACAGCGTAGA GCTGTTGAGC CCCGACATCA 
     1861 TCATGAATCT GCTTCTGTCA TACCGCGACG TCCAGGACTA CTCCGCCATC ATTGAGCTGG 
     1921 TGGAGACGCT GCAGGCTTTG CCCACCTGTG ATGTGGCCGA ACAGCACAAT GTCTGCTTCC 
     1981 ACTACACGTT TGCCCTCAAC AGGAGGAACA GGCCTGGGGA CCGTGAGAAG GCCCTGGCTG 
     2041 TCCTTCTGCC ATTGGTGAAG CATGAAGGCC CCGTGGCTCC AGACCTGTAC TGCATGTGTG 
     2101 GTCGTATCTA CAAGGACATG TTCTTCAGCT CCAGCTTCCA AAGTACTGGG CACCTGGAGC 
     2161 AGGCCTATCA CTGGTACCGC AAGGCATTTG ATGTGGAGCC CAGCCTACAC TCGGGCATCA 
     2221 ATGCAGCTGT GCTGCTCATT GCAGCTGGGA AACACTTTGA CGACTCTGAG GAGCTCCAGC 
     2281 TGATAGGCAT GAAGCTGGGC TGCCTGTTAG CCCGCAAAGG CTGTGTGGAG AAGATGCAGT 
     2341 ATTACTGGGA CGTGGGGTTC TACCTGGGAG CCCAGATTCT TGCCAACGAC CCCATCCAGG 
     2401 TGGTGCTGGC TGCAGAACAA CTATACAAGC TCAATGCCCC CATATGGTAC TTGGTGTCAG 
     2461 TGATGGAGAC CTTCTTACTG TACCAGCATT TCAGACCCAC ACCAGAGCCC TCGGGAGGAC 
     2521 CCGTGCTGCG AGCTCACTTT TGGCTCCATT TCTTGCTACA ATCCTGCCAG CCTTTCAAGG 
     2581 CGACCCCTCC TCAGGAGGAC CAGTGCCTGG TACTGGTGCT GGAGATAAAC AAGGTTCTGC 
     2641 TGCCTGCCAG ACTTGAGATT CAGGGAGCCG ACCCCATGAG CGCAGTGACC CTAAGCTTGC 
     2701 TGGAGCCAGA GACTCAGGAT GATCCTTCCA GCTGGACCTT CCCAGTCACC TCCATCTGTG 
     2761 GGATCAGCGC CTCCAAGCTG GACCAGCGCT GCTGCTTCCT GTACGCACTT CCTCCGGCCC 
     2821 AGGACGTCCA GCTGTGCTTT CCCAGCGTAG AGCGCTGCCG GTGGTTCTGT AGCCAAATTC 
     2881 AGGTCTTGGT GATGAATCCA GATTCCTCGG CGCCCACTGA GGAGGCAGAA GGCACAAGGG 
     2941 AGGTGCTGGA GTTTGATTAT GAATATTCGG AGACGGGTGA GCGGCTGGTG CTGGGCAAAG 
     3001 GCACGTACGG GGTGGTGTAT GCTGGCCGCG ACAGGCACAC GAGGGTACGA ATCGCCATCA 
     3061 AAGAGATCCC AGAGAGAGAT AGCAGATTCT CTCAGCCTCT GCACGAGGAG ATCGCTCTCC 
     3121 ACAAACGCCT TCGCCACAAG AATATAGTGC GCTATTTGGG CTCAGCCAGC CAGGGCGGCT 
     3181 ACCTCAAGAT CTTCATGGAG GAAGTGCCTG GAGGCAGCCT GTCCTCCTTG CTTAGGTCAG 
     3241 TGTGGGGACC CCTAAAGGAC AACGAGAGTA CTATTAGCTT CTACACGCGT CAGATCCTGC 
     3301 AAGGACTTAG CTACCTGCAT GAGAACCGCA TTGTTCACCG AGACATCAAG GGGGACAATG 
     3361 TACTGATCAA CACCTTCAGT GGGCTGCTCA AGATTTCTGA CTTTGGCACC TCCAAGCGGC 
     3421 TGGCAGGCAT CACACCCTGC ACGGAGACTT TCACAGGGAC CCTGCAGTAT ATGGCCCCAG 
     3481 AAATCATTGA CCAGGGCCCA CGAGGATATG GAAAGGCGGC TGACATCTGG TCTCTGGGCT 
     3541 GCACTGTGAT TGAGATGGCC ACAGGTTGGC CGCCCTTTCA CGAACTAGGG AGCCCGCAGG 
     3601 CTGCCATGTT TCAGGTGGGC ATGTACAAGG TACATCCACC AGTGCCAAGT TCCCTGTCAG 
     3661 CTGAGGCCCA AGCCTTCCTC CTCCGAACTT TTGAGCCAGA TCCCCGCCTC CGAGCCAGTG 
     3721 CCCAAGAGCT GTTGGGAGAC CCCTTCCTGC AACCAGGGAA GAGGAGCCGC AGCCCTGGCT 
     3781 CTCCTCGGCA CACTCCCCGG CCCTCAGGCA CCCCTTCCAG TACTTCCAGT CCTTCAGCTG 
     3841 ACTCAGCCAC ACAGTCTCAG ACATTCCCAA GGCCCCAGGC ACCCTCTCAG CACCCACCCA 
     3901 GCCCTCCGAA GCGCTGCCTT AGTTATGGGG ACACCAGTCA GCTCCGTGTG CCCGAGGAAT 
     3961 CCGCTGCCGA GGAACCCGCG TCCCCAGAAG AGAGTTCAGG CCTGAGCCTG CTGCACCAGG 
     4021 AGAGCAAGCG CCGAGCCATG CTGGCTGCAG TGCTGGAACA GGAGGTGCCC ATACTAGCAG 
     4081 AGAATCTCCT GGATCAGGAA CAGGATTCTC GACTCAGCAG GAATCATGTG GAACTTCTGC 
     4141 TTCGGTGCCT CGGGGCACAA ATCCATACTC CTAACCGCCG GCAGCTGGCC CAGGAGCTGC 
     4201 GGGCCCTGCA AGCTCAACTG CGGGCCCAGA GCCTGAGCCC TGCGCTTTTG AAGGGGCCGC 
     4261 TCTTCGCCTT TCCAGACGCG GTGAAGCAGA TCCTCCGCAG ACGCCAGATC CGCCCACACT 
     4321 GGATGTTCGT GTTGGACTCG CTGCTCAGCC GGGCAGTCCG GGCGGCGCTG GCCGTACTGG 
     4381 ACGCGGAGGC AGAGAAGAAA GCGGTCTCAC CGAAGTCAGA GGAGTGGAGT CAAGGGGAGT 
     4441 CCCAGCAGAA ACCGCTGGAG AGCCAGCCGC CGCTGCAGAG TCAGCTCCCA CCAGAGCAGG 
     4501 AACCTCCATC CCTGATGATA CAGCTGGGTC TCTTGCGAGC TGAGACTGAC AGGCTTCGGG 
     4561 ACATTCTGGC TGAGAAGGAA CGTGAGTGCC AGGCCCTCGT GCAACAGGCC CTGCATCGGG 
     4621 TGCACGCAGA GACCAGGACG TATGCCCTGG CTTCAGAGAC CCCGGCCACT CTCCCAAAGG 
     4681 ACCAGAAACT GGTGCAGTGG CTACAGGACC TGAGTGTAGA TCCAACCACT ATCCAAACGC 
     4741 TCCTGAGCCA TAGCTTTACC CTTCAAACCC TGCTCACCTG TGCCACTCAG GATGACCTCG 
     4801 TCTACACCAG AATCAGGGGA GGGATGGTAT GCAGAATTTG GAGAGCCATC TTGGCACAGC 
     4861 GAGCAGGAGC CACGTCGGTC ACCCCTGGAC CCCGGGATGC TGAGAGCGGA CCGACGCGTA 
     4921 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4981 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     5041 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     5101 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     5161 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     5221 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     5281 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     5341 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     5401 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5461 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5521 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5581 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5641 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5701 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5761 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5821 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5881 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5941 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     6001 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     6061 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     6121 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     6181 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     6241 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     6301 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     6361 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     6421 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6481 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6541 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6601 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6661 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6721 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6781 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6841 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6901 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6961 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     7021 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     7081 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     7141 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     7201 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     7261 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     7321 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     7381 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     7441 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7501 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7561 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7621 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7681 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7741 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7801 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7861 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7921 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7981 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     8041 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     8101 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     8161 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     8221 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     8281 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     8341 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     8401 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     8461 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8521 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8581 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8641 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8701 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8761 AATTTT
//