LOCUS       RR209068                6479 bp    DNA     circular  SYN 
DEFINITION  Ocln (Myc-DDK-tagged ORF) - Rat occludin (Ocln), (10 ug)
ACCESSION   NM_031329
VERSION     NM_031329.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2636..2727 
                     /label="Myc-DDK"  
     misc_feature    2757..3342 
                     /label="PolyA signal"  
     misc_feature    2814..2833 
                     /label="Restriction Enzyme"  
     misc_feature    3618..4290 
                     /label="ColE1"  
     misc_feature    4720..5514 
                     /label="Kan/Neo"  
     misc_feature    5549..5851 
                     /label="SV40 ori"  
     misc_feature    5937..6414 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2618..2624 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2618 
                     /label="Ocln"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCTGTGAGG CCTTTTGAGA GTCCACCTCC TTACAGGCCG GATGAATTCA 
     1081 AACCCAATCA TTATGCACCA AGCAATGACA TGTATGGCGG AGAGATGCAC GTTCGACCAA 
     1141 TGCTCTCTCA GCCGGCATAC TCTTTTTACC CGGAAGACGA AATTCTTCAC TTCTACAAGT 
     1201 GGACGTCGCC TCCAGGGGTG ATTCGGATCC TGTCTATGCT CGTCATCGTG ATGTGCATCG 
     1261 CTGTATTCGC CTGCGTGGCT TCCACACTTG CTTGGGACAG AGCCTATGGA ACGGGCATCT 
     1321 TTGGAGGAAG TATGAACTAC CCTTACGGCA GCGGCTTCGG TAGCTATGGA GGTGGCTTCG 
     1381 GAGGTTACGG TTACGGCTAC GGTTACGGCT ATGGAGGGTA CACAGACCCC AGAGCGGCAA 
     1441 AGGGTTTCCT GCTGGCCATG GCGGCCTTTT GCTTCATCGC TTCCTTGGTG ATCTTTGTTA 
     1501 CCAGCGTCAT TAGATCGGGA ATGTCCAGAA CGAGAAGATA CTACTTGATC GTGATCATAG 
     1561 TCAGCGCAAT CCTGGGCATC ATGGTGTTCA TTGCCACGAT CGTGTACATA ATGGGAGTCA 
     1621 ACCCGACTGC CCAGGCTTCT GGATCTATGT ACGGTTCACA GATCTATACC ATCTGCAGCC 
     1681 AGTTTTATAC TCCTGGAGGC ACTGGTCTCT ACGTGGACCA ATATTTGTAT CACTACTGTG 
     1741 TGGTGGATCC CCAGGAGGCT ATAGCCATTG TCCTGGGGTT CATGATTATT GTGGCTTTTG 
     1801 CTTTAATCAT TGTTTTTGCT GTGAAAACCC GAAGAAAGAT GGACCGGTAT GATAAGTCTA 
     1861 ATATTTTGTG GGATAAGGAA CACATTTATG ATGAACAGCC CCCTAATGTG GAAGAGTGGG 
     1921 TTAAAAATGT GTCTGCAGGC ACACAAGACA TGCCTCCACC CCCATCTGAC TATGCGGAAA 
     1981 GAGTCGACAG TCCAATGGCC TACTCCTCCA ACGGCAAAGT GAATGGCAAG AGATCCTACC 
     2041 CAGACTCTTT GTATAAGTCA CCGCCTCTGG TACCTGAAGT AGCCCAGGAG ATTCCTCTGA 
     2101 CCTTGTCCGT GGATGACTTC AGGCAGCCTC GGTACAGCAG CAACGATAAC CTAGAGACAC 
     2161 CTTCTAAAAG GACTCCCACG AAGGGGAAAG CAGGGAAGGC GAAGAGGACA GACCCAGACC 
     2221 ACTATGAAAC CGACTACACG ACAGGTGGCG AGTCCTGCGA TGAGCTGGAG GAGGACTGGC 
     2281 TCAGGGAATA TCCACCTATC ACTTCAGATC AACAGAGACA ACTGTACAAG AGAAATTTTG 
     2341 ATGCAGGTTT ACAGGAGTAT AAGAGTTTAC TGGCAGAACT CGACGAGGTC AACAAAGAGC 
     2401 TCTCTCGTCT CGACAGAGAA CTGGATGACT ACAGAGAGGA GAGCGAAGAG TACATGGCTG 
     2461 CTGCTGATGA ATATAACAGA CTAAAGCAAG TTAAGGGATC TGCAGATTAT AAAAGCAAGA 
     2521 AGAATTACTG CAAGCAGTTA AAGAGCAAAT TATCACACAT CAAGAGGATG GTGGGAGACT 
     2581 ATGACAGACG GAAAACCTGG TGGGAGACTA TGACAGACGG AAAACCACGC GTACGCGGCC 
     2641 GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA 
     2701 GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC 
     2761 CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC 
     2821 CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT 
     2881 CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA 
     2941 CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT 
     3001 GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT 
     3061 TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC 
     3121 GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC 
     3181 CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC 
     3241 TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC 
     3301 ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT 
     3361 CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC 
     3421 TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA 
     3481 GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT 
     3541 AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC 
     3601 CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT 
     3661 GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG 
     3721 CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG 
     3781 GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT 
     3841 CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG 
     3901 ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC 
     3961 GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA 
     4021 AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT 
     4081 GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT 
     4141 TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA 
     4201 TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC 
     4261 TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG 
     4321 AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG 
     4381 TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC 
     4441 CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC 
     4501 ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG 
     4561 TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG 
     4621 AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT 
     4681 GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT 
     4741 CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA 
     4801 CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG 
     4861 CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC 
     4921 GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT 
     4981 CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT 
     5041 GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT 
     5101 CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC 
     5161 GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA 
     5221 GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT 
     5281 CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT 
     5341 CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT 
     5401 GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT 
     5461 AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA 
     5521 TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA 
     5581 AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA 
     5641 TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG 
     5701 GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT 
     5761 TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG 
     5821 AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC 
     5881 TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT 
     5941 GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA 
     6001 ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA 
     6061 GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA 
     6121 GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT 
     6181 TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC 
     6241 ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT 
     6301 AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC 
     6361 AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC 
     6421 CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//