LOCUS       RR209031                8877 bp    DNA     circular  SYN 
DEFINITION  Synrg (Myc-DDK-tagged ORF) - Rat AP1 gamma subunit binding protein 1 (Ap1gbp1), (10 ug)
ACCESSION   NM_053419
VERSION     NM_053419.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5032..5123 
                     /label="Myc-DDK"  
     misc_feature    5153..5738 
                     /label="PolyA signal"  
     misc_feature    5210..5229 
                     /label="Restriction Enzyme"  
     misc_feature    6014..6686 
                     /label="ColE1"  
     misc_feature    7116..7910 
                     /label="Kan/Neo"  
     misc_feature    7945..8247 
                     /label="SV40 ori"  
     misc_feature    8333..8810 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5007..5014 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5007 
                     /label="Synrg"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGCTGCGG CCGGGAGCCG GAGCCAGCGG CGCGGCGGGG GCGGGCACAG 
     1081 GGCCCGGCGG GGCAGGCAGC TTCATGTTTC CTGTCGCAGG TGGCATGAGA CCCCCTCAAG 
     1141 GCCTGATTCC AATGCAGCAG CAAGGATTTC CTATGGTCTC TGTCATGCAG CCCAATATGC 
     1201 AAGGCATGAT GGGAATGAAT TACAGCTCTC AGATGTCCCA GGGACCCATT GCAATGCAGG 
     1261 CAGGAATACC AATGGGGCCA ATGCCAGCAG CGGGTGTACC TTTCCTGGGT CAGCCACCTT 
     1321 TTCTGGGCAT GCGTCCTGCA GCCCCACAGT ACACTCCAGA CATGCAGAAG CAGTTTGCGG 
     1381 AAGAGCAACA AAAACGATTT GAACAACAAC AAAAGCTCTT AGAAGAAGAA AGAAAACGAC 
     1441 GTCAGTTTGA AGAGCAAAAA CAAAAGCTCA GACTTCTGAG TAGCGTGAAA CCCAAGACAG 
     1501 GAGAGAAGAA CAGAGACGAC GCTTTGGAAG CCATCAAAGG AAACTTGGAT GGGTTTTCCA 
     1561 GAGATGCTAA GATGCACCCC ACTCCCGCAT CTCACCCAAA GAAACCAGAC TGCCCCACAT 
     1621 CATCTCATTC TACTAAAACT GTCTCCCCGT CATCTGCCTT TCTTGGTGAA GATGAATTCA 
     1681 GTGGCTTTAT GCAGGGGCCT GTTGAGCTTC CCACTTGTGG GCCTTCTTCC ACAGCCCAGC 
     1741 CCTTTCAGTC TTTCCTCCCC AGCACCCCAC TCGGCCAGTT ACACACACAG AAGGCTGGGG 
     1801 CACAGCCTCT ACCTCCAGGT CAGGCTCCAG TGTCTTTTGC CGTACATGGT GTCCATGGGC 
     1861 AAATTCCTTG TTTATCTGCT GCTTCAGCTT CCCACAGTAT GCAGAAAGCA GGCCCTTCCT 
     1921 TGGAAGAGAA GCTCCTAGTA TCTTGTGATA TAAGTGCATC TGGGCAGGAA CACATTAAAT 
     1981 TAAGCTCTCC TGAAGCTGGG CACAGAGCCG TAGTCCCAGG TTCCAGTAAG AACTCTCCCG 
     2041 GTTTAATGGC CCATAACGGG GGTGCTGTAG ATGGATGTGT GAGTGGTCCC ACCACTGCAG 
     2101 TCGCAGAAAA GACTTCAGAT CAAAACCTGT CAAAGGAAGA GAGTGGTGTG GGCGTGTTTC 
     2161 CCTCTCAGGA TCCTGTTCAG CCCAGAATGC CTCCTTGGAT TTACAATGAG AGTTTGGTTC 
     2221 CAGATGCTTA TAAGAAAATT TTAGAAACCA CAATGACTCC AACCGGAATA GACACTGCTA 
     2281 AACTTTATCC CATTCTGATG TCATCTGGAC TTCCCAGGGA AACTCTTGGA CAGATATGGG 
     2341 CCTTAGCTAA TCGAACTACG CCTGGCAAGC TCACTAAGGA GGAGCTTTAC ACCGTTCTCG 
     2401 CCATGGTAGC AGTAACACAG AGAGGTGTTC CTGCCATGAG CCCTGATACT TTGAACCAGT 
     2461 TCCCTGCGGC TCCCATTCCT ACCTTAAGCG GCTTTCCTAT GACTCTGCCT ACCCCAGTGA 
     2521 GCCAGCCAAC AGCGATGACC TCTGGCCCCG CAGGCTCCAT ACCCCTCAGC CTTGGACAGC 
     2581 CCATCATGGG CATTAACCTT GTTGGACCAG TGGGTGGAGC TGCAGCGCCA ACTTCCAGTG 
     2641 GCTTCATGCC AGCCTACCCC TCAAACCAGG TGGGAAAGAC AGAAGAAGAT GACTTCCAGG 
     2701 ATTTTCAAGA TGCTTCGAAG TCAGGATCCA TCGATGATTC TTTCACTGAC TTCCAAGAGG 
     2761 TGCCTGCGTC CTCAAAAACC AGTAATTCCC AGCATGGAAA CAGTGCTCCC TCACTCTTGA 
     2821 TCCCACTTCC TGGAACTAAA GCCTCCACAG ACAAATATGC AGTGTTTAAA GGGATTTCTG 
     2881 CTGAGAAGCC CTCTGAAAAC CCTGCTTCAT TTGGAGAGTC CGGTGATAAA TACAGTGCTT 
     2941 TCAGGGAACT CGAGCCAACA GCAGACAGTA AGCCTTTAGG AGAGAGCTTT GCGGAGTTCA 
     3001 GATCCACAGG AACCGATGAT GGCTTCACTG ACTTCAAGAC TGCTGATAGT GTGTCACCCC 
     3061 TAGAGCCACC AACAAAAGAC TCTTTTCCAT CGGCCTTTGC CTCAGGAGCT GCACAGCAGA 
     3121 CACAGACACA GGTGAAAACC CCTCTGAACT TAGCAGACCT AGATATGTTC TCCTCAGTGG 
     3181 ATTGCAGTGG GGAGAAACCA GTGCCCTTTT CAGCTGCATT TAGCACCTCA AAGTCAGTTT 
     3241 CCTCAAGACC TCAGCCGGCG GGTTCTGCAG CAGCCCCAGC ATCACTGGCA TCCACTAAAG 
     3301 CGTCCAGTTT GGCAGATGAT TTTGGAGAGT TCAACCTCTT TGGGGAATAT TCGAACCCTG 
     3361 CGTCTGTTGG GGAGCAGGAC GACTTTGCAG ATTTCATGGC TTTCGGTAAC AGCTCCATCC 
     3421 CCTCTGAGCC AAAAGCAGAT GACAAGTATG AAGCCCTCAG AGAAGAAGGG AGCCCCGGCG 
     3481 CCTTGTCCAC CAGCACTGTG GAGGGTGCAC ACAACCCACC TGTTTCGTCA TCCAAGTACG 
     3541 ACGTCTTCAA ACAGCTTTCC CTAGAGGGAG CTGGACTGGC CATCGAGGAG TTCAAAGAGA 
     3601 ACACTCCTTC CACAAAAAGT GACGGTGACT TTGCTGACTT CCACTCCAGT AAATTCTCAT 
     3661 CCACGAGCTC AGACAAATCC CTGGGAGAGA AAGCAGTGGC TTTCAGACAT GCCAAAGAAG 
     3721 ACTCCGCCTC GGTGAAGTCT TTAGACCTCC CCTCCATCGG TGGCAGCAGT GTTGGCAAGG 
     3781 AGGACTCTGA AGATGCGCTT TCTGTTCAGT TTGACATGAA GTTGGCTGAT GTAGGAGGAG 
     3841 ATCTTAAGCA TGTCATGTCT GATAGCTCTT TGGATTTACC AACAGTTAGT GGCCAACATC 
     3901 CTCCTGCCGC AGGATCAGCC TTGGCCAGTG AGGACGCTCT TCCAGAGACT CCCTTCCCAG 
     3961 CTTTTGCCAG CTTTAAAGAC ATGATGCCCC AGACCACTGA GCAGAAAGAG TATGAAAGTG 
     4021 GGGACTTCCA GGATTTCACA AGGCAGGACA TGCCCATGGT GGACCGGAGC CAAGAGAACA 
     4081 CATGTCCCAG CCCAGCTTCC AGTGTGGCCT CGCACGAAAC CCCCAAGGAG GGTGCAGACG 
     4141 ACTTTGGAGA ATTTCAAAGT GAAAAGCCCA AAATCAGCAA GTTTGACTTT TTAGTAGCTA 
     4201 ATTCACAGAG CAAAATGAAG TCTAGTGAAG AAATGATCAA AAGTGAGCTG GCAACCTTTG 
     4261 ACCTTTCTGT TCAAGGATCA CACAAGAGGA GCTTGAGCCT TGGGGATAAA GAAATAAGCA 
     4321 GGTCCTCTCC TTCCCCGGCC CTGGAGCAGC CCTTCAGAGA CCGGTCCAAC ACTCTGAGTG 
     4381 AGAGAGCTGC ACTGCCCGTC ATCCGAGACA AGTACAAGGA TCTGACTGGC GAAGTGGAGG 
     4441 AAAATGAGAG ATACGCGTAT GAGTGGCAGA GATGCCTGGG GAGCGCCCTG GATGTCATTA 
     4501 AGAAGGCAAA CGACACCTTA AACGGCATTA GCAGCAGTGC TGTCTGCACA GAAGTTATCC 
     4561 AGTCAGCCCA AGGCATGGAG TATCTCCTAG GCGTGGTGGA AGTGTACCGA GTAACCAAGC 
     4621 GCGTGGAGCT GGGGATCAAA GCCACTGCTG TGTGTAGTGA GAAACTCCAG CAGTTGCTGA 
     4681 AGGACATCGA TAAAGTGTGG AACAACTTAA TCGGCTTCAT GTCACTTACC ACACTCACGC 
     4741 CAGATGAGAA TTCACTGGAT TTTTCCTCCT GTATGTTACG GCCTGGGATC AAAAACGCCC 
     4801 AGGAGCTGGC TTGTGGAGTG TGTCTCCTAA ATGTGGACTC CAGAAGCCGG AAAGAAGAGA 
     4861 CACCTGCAGA AGAACAGCCT AAAAAAGCAT TCAACTCAGA AACAGACAGT TTCAAGCTGG 
     4921 CCTATGGGGG ACATCAGTAC CACGCCAGCT GTGCCAACTT CTGGATCAAC TGTGTTGAAC 
     4981 CCAAGCCTCC CGGCCTCCTC TTGCCAGATC TGCTCAGCGG ACCGACGCGT ACGCGGCCGC 
     5041 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     5101 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     5161 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     5221 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     5281 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     5341 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     5401 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     5461 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     5521 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     5581 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5641 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5701 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5761 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5821 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5881 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5941 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     6001 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     6061 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     6121 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     6181 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     6241 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     6301 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     6361 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     6421 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     6481 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     6541 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     6601 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6661 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6721 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6781 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6841 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6901 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6961 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     7021 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     7081 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     7141 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     7201 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     7261 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     7321 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     7381 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     7441 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     7501 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     7561 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     7621 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7681 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7741 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7801 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7861 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7921 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7981 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     8041 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     8101 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8161 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     8221 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     8281 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     8341 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     8401 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     8461 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     8521 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     8581 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     8641 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8701 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8761 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8821 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//