LOCUS       RR209008                8052 bp    DNA     circular  SYN 
DEFINITION  Herc4 (Myc-DDK-tagged ORF) - Rat hect domain and RLD 4 (Herc4), (10 ug)
ACCESSION   NM_001012074
VERSION     NM_001012074.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4209..4300 
                     /label="Myc-DDK"  
     misc_feature    4330..4915 
                     /label="PolyA signal"  
     misc_feature    4387..4406 
                     /label="Restriction Enzyme"  
     misc_feature    5191..5863 
                     /label="ColE1"  
     misc_feature    6293..7087 
                     /label="Kan/Neo"  
     misc_feature    7122..7424 
                     /label="SV40 ori"  
     misc_feature    7510..7987 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4191..4197 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4191 
                     /label="Herc4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTGTGCTGG GGAAACGCAT CCTATGGACA ACTAGGTTTG GGTGGAATTG 
     1081 ATGAAGAAAT TGTACTAGAG CCCAGGAAAA GTGACTTTTT CGTAAATAAA AAGGTCCGAG 
     1141 ATGTAGGATG TGGACTCAGA CATACTGTGT TCGTCCTGGA CGACGGAACT GTGTACACAT 
     1201 GTGGATGTAA TGATCTAGGA CAGCTAGGTC ATGAAAAGTC CAGAAAGAAA CCAGAGCAGG 
     1261 TTGTTGCCCT GGATGCCCAA AATATCGTAG CTGTTTCATG TGGAGAAGCT CATACATTAG 
     1321 CACTTAATGA CAAGGGCCAG GTGTATGCTT GGGGTCTCGA CTCTGATGGA CAGCTTGGCC 
     1381 TGCAAGGATC AGAGGAATGT ATCAGAGTAC CCAGAAATAT TAAAAGTCTT TCGGACATCC 
     1441 AGATAGTACA GGTTGCATGT GGTTACTATC ATTCACTTGC ACTTTCTAAA GCAAGTGAAG 
     1501 TTTTCTGTTG GGGACAGAAT AAATATGGCC AGCTCGGTCT CGGCATTGAA TGTCAAAAGC 
     1561 AAACCTCACC ACAGCTGATT AAGTCTTTGC TTGGAATACC ATTCATGCAA GTCGCAGCAG 
     1621 GAGGAGCCCA TAGTTTTGTA CTCACCCTTT CTGGAGCTAT CTTTGGATGG GGACGTAACA 
     1681 AATTTGGTCA GCTAGGTCTT AATGATGAGA ACGATAGGTA TGTTCCTAAT TTACTAAAGT 
     1741 CACTAAGATC TCAGAAAATA GTTTATATTT GTTGCGGAGA AGATCATACT GCTGCATTAA 
     1801 CCAAGGAAGG TGGAGTGTTT ACCTTTGGAG CTGGAGGCTA TGGCCAGCTG GGTCATAATT 
     1861 CTACCAGTCA TGAAATAAAC CCCAGGAAAG TGTTTGAGCT TATGGGAAGC ATTGTTACTC 
     1921 AAATTGCTTG TGGAAGGCAG CATACTTCTG CATTTGTTCC TTCTTCAGGA CGAATTTACT 
     1981 CCTTCGGTCT CGGTGGTAAT GGGCAGTTGG GAACTGGTTC AACAAGCAAC AGAAAAAGCC 
     2041 CTTTCACTGT AAAAGGAAAC TGGTTTTCTT ATAATGGACA ATGTCCACAA GATATTGGTT 
     2101 CTGAAGACTA TTTCTGTGTC AAAAGAATTT TCTCAGGTGG AGATCAAAGC TTTTCACATT 
     2161 ACTCTAGTCC TCAGAGCTGT GGACCTCCAG ATGACTTTAG ACGCTCTGAC CCTTCAAAGC 
     2221 AGATCTGGAC AGTGAATGAA GCTCTGATTC AGAAATGGCT CAGCTACCCC TCTGGAAGGT 
     2281 TCCCTGTGGA GATAGCCAAT GAGATAGATG GAACATTTTC TTCGTCTGGT TGCCTAAATG 
     2341 GAAGTTTTCT GGCTGTTAGC AATGATGATC ACTATAGAAC AGGCACCAGA TTTTCAGGGG 
     2401 TCGACATGAA TGCCGCTAGA CTCTTATTCC ACAGACTCAT ACAGCCTGAC CACCCGCAGA 
     2461 TATCTCAGCA GGTGGCAGCT AGTTTGGAAA AGAACCTTAT CCCTAAACTG ACTAGTTCCT 
     2521 TACCTGATGT TGAAGCTTTG AGGTTTTATC TTACCCTGCC AGAGTGCCCC CTGATGAGTG 
     2581 ACTGCAACAA TTTCACAACC ATAGCAATCC CCTTTGGCAC AGCCCTTGTG AACCTAGAAA 
     2641 AAGCACCACT GAAAGTACTT GAAAACTGGT GGTCAGTACT TGAACCTCCA CTATTCCTCA 
     2701 AGATAGTAGA ACTTTTTAAG GAAGTTGTGG TACATCTTTT GAAACTCTAC AAGATCGGCA 
     2761 TTCCCCCTTC TGAAAGAAGA ATTTTCAACA GTTTTCTTCA TACCGCATTA AAGGTTTTAG 
     2821 AAATATTACA TAGGGTAAAT GAGAGAACAG GACAACTTAT ACAGTACGAC AAGTTTTACA 
     2881 TACATGAAGT CCAAGAGCTG ATAGACATAA GGAATGACTA TATCAACTGG GTCCAGCAGC 
     2941 AAGCCTATGG AGTGGATGTC AGCCATGGAA TAACTGAGTT GGCAGATATC CCTGTGACAA 
     3001 TTTGTACATA TCCATTTGTA TTTGATGCCC AGGCAAAAAC TACTCTGCTA CAGACAGACG 
     3061 CAGTCTTGCA GATGCAGATG GCTATTGACC AGGCCCATAG GCAGAATGTC TCCTCTCTTT 
     3121 TTCTGCCAGT GATTGAGTCT GTGAATCCTT GCTTAATTCT GGTTGTTCGC AGAGAAAATA 
     3181 TTGTAGGAGA TGCAATGGAA GTCCTCAGGA AAACCAAGAA TATAGATTAT AAAAAGCCAC 
     3241 TCAAAGTTAT ATTTGTTGGA GAAGATGCTG TTGATGCTGG AGGTGTACGC AAAGAATTTT 
     3301 TCTTGCTCAT CATGAGGGAA TTATTGGATC CTAAATATGG CATGTTTCGA TATTATGAAG 
     3361 ATTCCAGGCT TATTTGGTTT TCAGATAAGA CATTTGAAGA CAGTGATTTG TTCCACTTAA 
     3421 TTGGTGTTAT CTGTGGATTA GCAATTTATA ATTTTACTAT TGTGGACCTC CACTTCCCTT 
     3481 TGGCTTTATA TAAGAAGCTA CTGAAAAGGA AGCCATCCCT GGATGATCTG AAAGAGCTGA 
     3541 TGCCAGATGT AGGGAGAAGC ATGCAACAAT TGCTGGACTA CCCGGAGGAC GATATAGAGG 
     3601 AAACATTTTG TCTAAACTTT ACGATCACAG TTGAAAATTT TGGTGCAACA GAAGTGAAAG 
     3661 AGCTGGTTCT GAATGGTGCA GACACCGCTG TTAACAAACA GAATCGGCAG GAGTTTGTTG 
     3721 ATGCCTATGT GGATTACATA TTCAATAAAT CAGTGGCATC TTTATTTGAT GCTTTCCATG 
     3781 CCGGCTTTCA TAAGGTCTGT GGAGGAAAAG TTCTTCTGCT CTTCCAGCCC AATGAATTAC 
     3841 AAGCTATGGT TATTGGGAAT ACAAATTATG ATTGGAAAGA ACTGGAGAAG AATACAGAAT 
     3901 ACAAAGGAGA GTATTGGGCA GAACATCCCA CGATAAAAAT TTTTTGGGAA GTATTTCATG 
     3961 AATTACCACT AGAAAAGAAG AAACAATTCC TATTATTTTT GACAGGTAGT GATCGAATTC 
     4021 CTATTCTTGG TATGAAGAGT CTGAAACTAG TCATCCAGTC AACAGGAGGT GGTGAGAGCT 
     4081 ATCTCCCGGT TTCCCATACC TGTTTTAATC TTCTAGATCT CCCAAAATAT ACAGAGAAAG 
     4141 AAACCCTACG ATGTAAACTG ATCCAAGCTA TTGATCACAA TGAAGGCTTC AGTTTAATAA 
     4201 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     4261 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4321 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4381 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4441 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4501 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     4561 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     4621 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     4681 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     4741 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     4801 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     4861 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     4921 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     4981 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     5041 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     5101 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     5161 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     5221 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     5281 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     5341 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     5401 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     5461 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     5521 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     5581 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     5641 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     5701 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     5761 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     5821 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     5881 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     5941 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     6001 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     6061 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     6121 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     6181 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     6241 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     6301 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     6361 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     6421 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     6481 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     6541 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     6601 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     6661 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     6721 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     6781 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     6841 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     6901 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     6961 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     7021 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     7081 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     7141 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     7201 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     7261 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     7321 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     7381 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7441 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     7501 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     7561 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     7621 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     7681 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     7741 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     7801 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     7861 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     7921 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     7981 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     8041 AACGCGAATT TT
//