LOCUS       RR208980                9459 bp    DNA     circular  SYN 
DEFINITION  Top2a (Myc-DDK-tagged ORF) - Rat topoisomerase (DNA) II alpha (Top2a), (10 ug)
ACCESSION   NM_022183
VERSION     NM_022183.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5616..5707 
                     /label="Myc-DDK"  
     misc_feature    5737..6322 
                     /label="PolyA signal"  
     misc_feature    5794..5813 
                     /label="Restriction Enzyme"  
     misc_feature    6598..7270 
                     /label="ColE1"  
     misc_feature    7700..8494 
                     /label="Kan/Neo"  
     misc_feature    8529..8831 
                     /label="SV40 ori"  
     misc_feature    8917..9394 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5598..5604 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5598 
                     /label="Top2a"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAAGTGTCA CCATTGCAGC CTGTAAATGA AAATATGCTA CTCAACAAAA 
     1081 AGAAGAATGA AGATGGCAAG AAAAGACTGT CCGTTGAAAG AATCTATCAG AAAAAAACCC 
     1141 AACTGGAACA TATACTGCTC CGCCCAGATA CCTACATCGG CTCTGTGGAG TTAGTGACCC 
     1201 AGCAAATGTG GGTTTACGAT GAAGATGTTG GTATTAACTA CAGGGAAGTC ACTTTTGTTC 
     1261 CTGGTTTGTA TAAAATCTTT GATGAAATTC TAGTTAATGC TGCTGATAAC AAACAAAGGG 
     1321 ACCCAAAAAT GTCTTGCATT AGAGTCACAA TGATGCGAAA TAATTTAATT AGTATATGGA 
     1381 ATAATGGAAA GGGAATTCCT GTTGTTGAAC ACAAAGTGGA GAAAATGTAT GTCCCAGCTC 
     1441 TCATATTTGG ACAGCTCCTG ACCTCCAGTA ACTACGATGA TGATGAGAAG AAAGTGACAG 
     1501 GTGGTCGAAA TGGCTATGGA GCTAAACTGT GTAACATATT CAGCACCAAA TTTACTGTGG 
     1561 AAACAGCCAG TAGGGAATAC AAGAAAATGT TCAAACAGAC ATGGATGGAT AACATGGGGA 
     1621 GAGCTGGTGA CATGGAGCTC AAGCCCTTTA GCGGAGAGGA TTACACGTGT ATTACCTTCC 
     1681 AGCCTGACTT ATCTAAGTTT AAAATGCAAA GCCTGGACAA AGATATTGTC GCACTGATGG 
     1741 TCAGAAGAGC ATACGATATT GCTGGTTCCA CCAAAGATGT AAAAGTCTTT CTTAATGGAA 
     1801 ACAGGCTGCC AGTCAAAGGG TTCCGTAGTT ACGTGGATAT GTATTTGAAG GATAAGGTTG 
     1861 ATGAAACTGG GAACGCTCTG AAGGTGGTGC ATGAACAAGT AAATCCCCGG TGGGAAGTGT 
     1921 GCTTAACAAT GAGCGAGAAA GGCTTTCAGC AGATTAGCTT CGTCAACAGC ATCGCTACTT 
     1981 CTAAGGGTGG CAGACATGTC GATTATGTAG CCGATCAGAT TGTGAGTAAA CTCGTTGATG 
     2041 TGGTGAAAAA GAAGAACAAG GGAGGTGTTG CAGTGAAGGC CGATCAGGTG AAGAATCACA 
     2101 TGTGGATTTT TGGAAATGCG GTAATTGAAA ACCCAACCTT TGATTCTCAA ACAAAAGAAA 
     2161 ACATGACTTT ACAAGCCAAG AGCTTTGGAT CAACTTGTCA ATTAAGTGAA AAATTCATCA 
     2221 AAGCTGCAAT TGGTTGTGGT ATTGTCGAAA GCATACTAAA CTGGGTGAAG TTTAAGGCCC 
     2281 AAATCCAATT AAACAAGAAG TGTTCAGCTG TAAAACATAA CAGGATCAAG GGAATCCCCA 
     2341 AACTTGACGA CGCCAATGAC GCAGGGAGCC GAAACTCTGC TGAATGCACA CTTATCCTGA 
     2401 CTGAAGGGGA CTCAGCCAAA ACTCTGGCAG TCTCAGGACT TGGAGTGGTT GGAAGAGACA 
     2461 AATATGGGGT GTTCCCTCTC AGAGGAAAAA TACTCAATGT GCGAGAAGCT TCTCATAAAC 
     2521 AGATCATGGA AAATGCTGAA ATTAACAATA TCATCAAGAT TGTGGGGCTT CAGTACAAGA 
     2581 AAAACTATGA GGATGAAGAT TCATTGAAAA CTCTTCGTTA TGGGAAGATC ATGATTATGA 
     2641 CAGATCAGGA CCAAGATGGT TCCCACATCA AAGGCTTGCT GATCAATTTT ATCCATCACA 
     2701 ATTGGCCATC TCTTCTGCGA CATCGTTTTC TAGAAGAATT TATCACTCCT ATTGTAAAGG 
     2761 TATCTAAAAA CAAGCAAGAA ATAGCATTCT ACAGTCTTCC CGAGTTTGAA GAATGGAAGA 
     2821 GTACAAATCC AAATCACAAA AAATGGAAAG TCAAGTACTA CAAAGGTTTG GGCACGAGCA 
     2881 CATCAAAGGA AGCTAAGGAG TATTTTGCAA ATATGAAACG ACATCGTATT CAGTTCAAAT 
     2941 ACTCTGGCCC TGAAGACGAT GCTGCGATCA GCCTGGCCTT TAGTAAAAAA CAGGTCGATG 
     3001 ATCGAAAGGA ATGGTTAACT AATTTCATGG AAGATAGAAG ACAGCGGAAG TTACTTGGCC 
     3061 TTCCTGAGGA TTATTTGTAT GGACAAACCA CTATGTACCT GACCTATAAT GATTTCATTA 
     3121 ACAAGGAACT TATCCTCTTT TCAAACTCTG ATAATGAAAG ATCTATCCCA TCTATGGTAG 
     3181 ATGGTTTGAA ACCAGGTCAG AGAAAGGTTT TGTTTACTTG TTTCAAACGG AATGACAAGC 
     3241 GAGAGGTGAA AGTTGCCCAG TTAGCTGGGT CAGTGGCAGA AATGTCCTCT TATCACCATG 
     3301 GTGAGATGTC GCTGATGATG ACCATTATCA ATTTGGCTCA GAATTTTGTG GGTAGCAATA 
     3361 ATTTGAACCT TCTACAGCCT ATTGGTCAGT TTGGTACCAG GCTGCATGGT GGCAAGGACT 
     3421 CAGCCAGCCC TAGGTACATC TTTACAATGC TCAGCCCTTT GGCTCGGTTG TTATTTCCAT 
     3481 CAAAAGATGA TCACACGTTA CGGTTTCTGT ATGACGACAA CCAGCGTGTT GAGCCTGAAT 
     3541 GGTACATCCC TATTATACCC ATGGTGCTGA TAAATGGTGC TGAGGGAATT GGTACTGGGT 
     3601 GGTCCTGCAA AATCCCCAAC TTTGATGTTC GTGAAGTCGT AAATAATATC AGGCGGCTGT 
     3661 TGGATGGAGA AGAGCCTCTG CCCATGCTCC CAAGTTATAA GAATTACAAA GGTACTATTG 
     3721 AAGAACTGGC TTCAAATCAG TATGTGATTA ATGGAGAAGT AGCTATTCTG AATTCCACAA 
     3781 CCATTGAGAT CACTGAGCTT CCCATCAGAA CATGGACCCA GACATATAAG GAACAGGTTC 
     3841 TAGAACCTAT GTTGAATGGC ACCGAGAAGA CACCCCCTCT AATAACAGAC TATAGGGAAT 
     3901 ACCACACAGA TACCACTGTG AAGTTTGTCA TAAAGATGAC TGAAGAAAAA TTGGCAGAGG 
     3961 CAGAGAGAGT GGGACTACAC AAAGTCTTCA AACTCCAGAC TAGTCTCACT TGCAACTCTA 
     4021 TGGTACTTTT TGACCATGTT GGTTGTTTAA AGAAATATGA CACTGTGTTG GATATTCTAA 
     4081 GAGACTTCTT TGAGCTCAGG CTTAAATATT ATGGATTAAG AAAAGAATGG CTTCTAGGAA 
     4141 TGCTTGGTGC AGAATCTTCT AAACTGAATA ATCAAGCTCG CTTTATATTA GAGAAAATAG 
     4201 ATGGCAAAAT AGTCATTGAA AATAAACCTA AGAAAGAATT AATTAAAGTT CTGATTCAGA 
     4261 GAGGCTATGA CTCCGACCCT GTGAAAGCCT GGAAAGAAGC TCAGCAGAAG GTCCCAGAAG 
     4321 AAGAGGAAAA TGAAGAGAAT GAAGAGAGCG AGAGTGAAAG CACGAGCCCT GCAGCGGAGT 
     4381 CTGGACCGAC CTTCAACTAC CTTCTTGATA TGCCCCTGTG GTATCTAACC AAGGAGAAGA 
     4441 AGGACGAGCT GTGCAAGCAA AGGGACGAGA AGGAACAAGA GCTCAACACG TTAAAGAAAA 
     4501 AGACTCCATC GGATTTGTGG AAGGAAGACC TGGCTGCTTT TGTGGAAGAA CTGGAGGTTG 
     4561 TTGAAGCCAA GGAAAAACAG GATGAACAAG TAGGACTTCC TGGAAAGGGG GTGAAAGCGA 
     4621 AGGGGAAGAA AGCGCAGATA TCTGAAGTCC TGCCTTCTCC TGTGGGGAAA AGGGTCATCC 
     4681 CGCAGGTGAC TATGGAGATG AGAGCTGAGG CAGAGAAGAA AATCAGGAGG AAAATTAAGA 
     4741 GCGAGAATGT TGAGGGCACC CCTGCTGAGG ATGGTGCTGA GCCAGGACTC CGGCAGAGAC 
     4801 TAGAGAAGAG GCAGAAGAGA GAGCCAGGTA CCAGGGCAAA GAAGCAAACT ACATTGCCAT 
     4861 TTAAGCCTAT CAAAAAAGCC CAAAAGCAAA ACCCTTGGTC TGATTCTGAA TCAGATATGA 
     4921 GCAGCAACGA AAGCAATTTT GATGTCCCTC CACGAGAGAA AGAGCCAAGA ATTGCTGCAA 
     4981 CAAAAGCCAA ATTCACGGCG GATTTAGACT CTGATGACGA TTTCTCAGGC TTGGATGAGA 
     5041 AGGATGAGGA TGAAGATTTC TTCCCATTAG ATGATACTCC ACCTAAGACC AAAATGCCCC 
     5101 CAAAAAATAC TAAAAAAGCA CTGAAGCCAC AGAAGAGCTC CACATCAGTT GATCTTGAAA 
     5161 GTGATGGGAA AGACAGTGTA CCAGCTTCTC CAGGCGCTTC TGCTGCTGAC GTCCCAGCTG 
     5221 AGACTGAACC CTCCAAGCCG TCCTCCAAAC AGACAGTGGG AGTGAAGAGG ACAATAACCA 
     5281 AAGGTCAGTC TTTAACCTCC ACTGCTGGCA CCAAAAAGAG AGCTGTACCA AAGGAGACCA 
     5341 AGTCAGATTC AGCCTTGAAT GCTCATGTCT CCAAAAAGCC TGCTCCTGCC AAAGCCAAGA 
     5401 ACAGTCGCAA GAGGATGCCA TCTTCTTCTG ACAGCTCGGA CTCTGAGTTT GAGAAGGCGA 
     5461 TTTCTAAAGG TGCCACAAGC AAGAAACTCA AGGGAGAGGA GCGGGATTTC CATGTGGACT 
     5521 TAGATGACAC TGTGGCTCCT CGAGCAAAAT CCGGACGAGC AAGGAAGCCG ATTAAGTACC 
     5581 TGGAGGAGTC GGATGACGAC CTCTTCACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     5641 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     5701 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     5761 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     5821 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     5881 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     5941 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     6001 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     6061 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     6121 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     6181 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     6241 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     6301 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     6361 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     6421 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     6481 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     6541 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     6601 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     6661 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     6721 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     6781 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     6841 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     6901 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     6961 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     7021 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     7081 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     7141 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     7201 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     7261 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     7321 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     7381 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     7441 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     7501 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     7561 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     7621 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     7681 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     7741 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     7801 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     7861 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     7921 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     7981 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     8041 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     8101 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     8161 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     8221 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     8281 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     8341 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     8401 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     8461 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     8521 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     8581 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     8641 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     8701 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     8761 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     8821 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     8881 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     8941 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     9001 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     9061 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     9121 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     9181 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     9241 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     9301 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     9361 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     9421 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//