LOCUS       RR208942                8721 bp    DNA     circular  SYN 
DEFINITION  Dctn1 (Myc-DDK-tagged ORF) - Rat dynactin 1 (Dctn1), (10 ug)
ACCESSION   NM_024130
VERSION     NM_024130.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4878..4969 
                     /label="Myc-DDK"  
     misc_feature    4999..5584 
                     /label="PolyA signal"  
     misc_feature    5056..5075 
                     /label="Restriction Enzyme"  
     misc_feature    5860..6532 
                     /label="ColE1"  
     misc_feature    6962..7756 
                     /label="Kan/Neo"  
     misc_feature    7791..8093 
                     /label="SV40 ori"  
     misc_feature    8179..8656 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4860..4866 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4860 
                     /label="Dctn1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCCAGAGC AAGAGGCACA TGTACAATCG GACGCCCAGT GGCAGCAGGA 
     1081 TGAGTACGGA GGCAAGCGCC CGGCCCCTGC GGGTCGGCTC CCGTGTGGAG GTGATTGGGA 
     1141 AGGGCCACCG TGGTACTGTG GCCTATGTTG GAGCTACACT CTTTGCCACT GGCAAGTGGG 
     1201 TGGGCGTGAT CCTGGATGAA GCAAAAGGCA AGAACGATGG TACCGTTCAG GGAAGGAAGT 
     1261 ATTTCACATG CGATGAAGGC CACGGCATCT TTGTACGCCA ATCCCAGATC CAAGTATTTG 
     1321 AAGATGGAGC AGATACTACT TCCCCAGAGA CTCCTGATTC TTCTGCTTCA AAGATCCTCA 
     1381 AGAGAGAGGG AGCTGATGCA GCTGCAAAGA CCAGCAAACT GCGGGGACTG AAGCCTAAGA 
     1441 AGGCACCGAC AGCCCGAAAG ACCACAACTC GACGGCCCAA GCCTACTCGC CCAGCCAGTA 
     1501 CTGGGGTGGC TGGGCCCAGT AGCTCCTTGG GCCCCTCTGG ATCAGCATCA GCCGGTGAAC 
     1561 TGAGCAGCAG TGAGCCCAGC ACCCCAGCTC AGACTCCGCT GGCAGCACCC ATCATCCCCA 
     1621 CACCGGCCCT CACCTCTCCT GGAGCAGCAC CCCCACTTCC TTCTCCCTCT AAGGAAGAGG 
     1681 AAGGGCTGAG GGACCAGGTA CGGGACCTGG AGGAGAAGCT GGAGACCTTG CGTCTGAAAC 
     1741 GCTCAGAAGA CAAAGCAAAG CTGAAGGAGC TGGAGAAGCA CAAGATCCAG CTGGAGCAGG 
     1801 TGCAGGAATG GAAGAGCAAA ATGCAGGAGC AGCAGGCGGA CCTGCAGCGG CGCCTCAAGG 
     1861 AGGCGAAGGA AGCCAAGGAG GCACTGGAGG CAAAGGAGCG CTACATGGAG GAGATGGCAG 
     1921 ACACAGCCGA CGCCATCGAG ATGGCCACTC TGGACAAGGA GATGGCCGAG GAGCGGGCCG 
     1981 AGTCCCTGCA GCAGGAGGTG GAGGCACTGA AGGAACGAGT AGACGAACTC ACCACAGACT 
     2041 TGGAGATCCT CAAGGCTGAA ATTGAAGAGA AAGGCTCTGA TGGGGCTGCG TCCAGCTACC 
     2101 AGCTCAAACA GCTGGAGGAG CAGAATGCCC GCCTGAAGGA CGCCCTGGTG AGGATGCGAG 
     2161 ATCTCTCTTC CTCAGAGAAG CAGGAGCATG TGAAGCTCCA GAAGCTCATG GAAAAGAAGA 
     2221 ACCAGGAGCT GGAGGTTGTG AGGCAGCAGC GTGAGCGTCT TCAGGAGGAA CTGAGCCAGG 
     2281 CAGAGAGCAC CATCGATGAG CTCAAAGAGC AGGTGGACGC TGCTCTGGGA GCTGAGGAGA 
     2341 TGGTGGAGAT GCTGACTGAC CGGAACCTGA ATCTGGAGGA GAAAGTGCGA GAATTACGGG 
     2401 AGACTGTGGG GGACTTGGAA GCGATGAATG AGATGAATGA CGAGCTGCAG GAGAACGCAC 
     2461 GCGAGACAGA ACTGGAGCTG CGTGAGCAGC TGGACATGGC AGGCGCCCGA GTAAGGGAGG 
     2521 CCCAGAAGCG AGTGGAAGCC GCCCAGGAGA CGGTCGCCGA CTACCAGCAG ACCATCAAGA 
     2581 AGTACCGCCA GTTGACTGCC CACCTACAGG ATGTCAATCG GGAGCTGACC AACCAGCAGG 
     2641 AAGCGTCTGT GGAGAGACAG CAGCAGCCAC CGCCAGAGAC TTTTGATTTC AAAATCAAGT 
     2701 TTGCTGAGAC CAAGGCTCAT GCCAAGGCGA TCGAGATGGA GTTGAGGCAG ATGGAAGTTG 
     2761 CCCAGGCCAA CCGGCACATG TCCCTGCTGA CGGCCTTTAT GCCTGACAGC TTCCTTCGGC 
     2821 CAGGGGGAGA CCATGACTGT GTCCTGGTGC TGCTGCTCAT GCCCCGACTC ATCTGCAAGG 
     2881 CAGAGCTCAT CCGGAAGCAG GCCCAGGAGA AGTTTGACCT GAGTGAGAAC TGTTCGGAGC 
     2941 GGCCTGGGCT GCGAGGGGCT GCTGGGGAGC AGCTAAGCTT TGCCGCCGGG CTGGTGTACT 
     3001 CGCTGAGTCT GCTGCAGGCC ACACTGCACC GCTACGAGCA TGCCCTCTCT CAGTGCAGCG 
     3061 TGGACGTGTA CAAGAAGGTA GGCAGCCTGT ACCCGGAGAT GAGTGCCCAC GAGCGCTCCT 
     3121 TAGATTTCCT CATCGAGCTG CTGCACAAGG ATCAGCTGGA CGAGACTGTC AACGTGGAGC 
     3181 CCCTCACCAA GGCCATCAAG TACTACCAGC ATCTGTACAG CATCCACCTC GCTGAGCAGC 
     3241 CCGAGGAGTC CACCATGCAG CTGGCTGACC ACATCAAGTT CACCCAGAGT GCCCTGGATT 
     3301 GTATGAGCGT GGAGGTGGGG CGGCTGCGTG CCTTCTTGCA GGGTGGGCAG GAGGCAACAG 
     3361 ATATTGCTCT TCTTCTGCGA GACCTGGAAA CATCCTGCAG TGACATCCGT CAGTTCTGCA 
     3421 AGAAGATCCG ACGGCGAATG CCAGGGACGG ATGCTCCTGG GATCCCAGCA GCGCTGGCCT 
     3481 TCGGCTCACA GGTGTCTGAC ACACTCCTGG ACTGCAGGAA GCACCTGACG TGGGTGGTAG 
     3541 CTGTTCTGCA GGAGGTGGCA GCTGCAGCCG CCCAGCTCAT CGCCCCCTTG GCAGAGAACG 
     3601 AGGGGCTGCC CGTGGCTGCA CTGGAGGAGC TGGCTTTCAA AGCAAGCGAG CAGATCTACG 
     3661 GGAGCCCCTC CAGCAGCCCC TACGAATGTC TGCGCCAGTC ATGCTCCATC CTCATCAGCA 
     3721 CGATGAACAA GTTGGCCACA GCCATGCAGG AAGGCGAGTA TGACGCAGAG CGGCCCCCGA 
     3781 GCAAGCCTCC TCCAGTTGAA CCTTGGCCTG CAGCACTGCG TGCAGAGATC ACAGATGCTG 
     3841 AAGGTCTGGG TTTGAAGCTT GAGGATCGAG AGACAGTCAT CAAGGAGTTA AAGAAGTCAC 
     3901 TCAAGATTAA GGGAGAGGAG CTGAGTGAGG CCAACGTGCG GCTGAGCCTC CTGGAGAAGA 
     3961 AGCTGGACAG CGCTGCCAAG GATGCTGATG AGCGAATCGA GAAAGTTCAG ACTCGGCTGG 
     4021 AGGAGACGCA GACCCTGCTA CGGAAGAAGG AGAAAGAGTT TGAGGAGACA ATGGATGCAC 
     4081 TCCAGGCTGA CATAGACCAG CTGGAGGCAG AGAAGACAGA GCTCAAGCAG CGCCTGAACA 
     4141 GCCAGTCCAA GCGCACTATT GAGGGACTCC GGGGCCCCCC TCCATCAGGC ATTGCTACCC 
     4201 TGGTCTCTGG CATTGCTGGT GAGGAACAAC AGCGAGGGGG CACTCCTGGG CAGGCTCCAG 
     4261 GCGCCTTGCC AGGCCCGGGG CCGGTGAAGG ACTCACCACT GCTGCTTCAG CAGATCTCTG 
     4321 CCATGAGGTT ACACATCTCT CAGCTCCAGC ATGAGAACAG CATCCTCAGA GGAGCCCAGA 
     4381 TGAAGGCGTC CTTGGCAGCT CTGCCCCCTC TGCATGTTGC AAAGTTTTCC CTCCCACCCC 
     4441 ATGAGGGCCC TGGTGGTAAC CTCCTGTCTG GGGCACTGTA TCGCAAGACC AGCCAGCTGT 
     4501 TGGAGAAACT AAACCAACTG AGCACTTACA CCCACGTAGT AGACATCACC CGAAGCAGCC 
     4561 CGGCCTGCAA GAGCCCATCC GCTCAGCTTA TGGAACAAGT GGCCCAGCTC AAGTCTCTGA 
     4621 GCGACACCAT CGAGAAGCTC AAGGATGAGG TCCTCAAGGA GACAGTAACT CAGCGTCCTG 
     4681 GAGCTACTGT TCCCACTGAC TTTGCCACCT TCCCTTCATC TGCCTTCCTG AGGGCCAAGG 
     4741 AAGAGCAGCA AGACGACACA GTCTACATGG GCAAAGTGAC CTTCTCGTGT GCGGCAGGCC 
     4801 TCGGACAGCG ACACCGCCTG GTGCTGACCC AGGAGCAGCT GCACCAGCTT CACGGTCGTC 
     4861 TCATCTCCAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4921 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4981 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     5041 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     5101 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     5161 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     5221 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     5281 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     5341 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5401 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5461 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5521 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5581 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5641 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5701 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5761 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5821 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5881 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5941 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     6001 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     6061 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     6121 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     6181 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     6241 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     6301 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     6361 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6421 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6481 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6541 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6601 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6661 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6721 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6781 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6841 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6901 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6961 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     7021 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     7081 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     7141 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     7201 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     7261 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     7321 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     7381 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7441 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7501 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7561 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7621 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7681 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7741 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7801 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7861 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7921 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7981 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8041 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8101 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     8161 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     8221 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     8281 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     8341 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     8401 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     8461 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8521 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8581 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8641 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8701 CAAAAATTTA ACGCGAATTT T
//