LOCUS       RR208848                7944 bp    DNA     circular  SYN 
DEFINITION  Clmn (Myc-DDK-tagged ORF) - Rat calmin (Clmn), (10 ug)
ACCESSION   NM_001106755
VERSION     NM_001106755.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4101..4192 
                     /label="Myc-DDK"  
     misc_feature    4222..4807 
                     /label="PolyA signal"  
     misc_feature    4279..4298 
                     /label="Restriction Enzyme"  
     misc_feature    5083..5755 
                     /label="ColE1"  
     misc_feature    6185..6979 
                     /label="Kan/Neo"  
     misc_feature    7014..7316 
                     /label="SV40 ori"  
     misc_feature    7402..7879 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4083..4089 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4083 
                     /label="Clmn"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTGCTCAG GAGTGGGACT GGTTCCAGCG CGAGGAGCTC ATCGGGCAGA 
     1081 TCAGCGACAT CCGTGTGCAG AACCTGCAAG TGGAGAGGGA AAACGTGCAG AAGAGGACTT 
     1141 TCACACGATG GATAAACCTA CACCTCGAAA AGTGCGACCC ACCTCTAGAA GTTACGGACC 
     1201 TGTTTGTTGA TATCCAAGAT GGCAAAATCC TAATGGCTTT GTTAGAAGTT CTCTCTGGGC 
     1261 GAAATCTGCT ACATGAATAC AAATCCTCAT CACATCGTAT TTTTCGGTTG AACAACATAG 
     1321 CGAAAGCACT CAAGTTTCTG GAAGATAGCA ATGTCAAGCT GGTTAGCATC GACGCGGCAG 
     1381 AGATAGCTGA CGGCAACCCC TCGCTGGTTC TCGGGCTGAT ATGGAATATC ATCCTCTTCT 
     1441 TCCAGATAAA GGAGCTCACA GGCAACCTCA GCAGGAGTTC TCCATCTTCC AGCTTGTCAC 
     1501 CTGGCTCCGG GGGCACCGAC TCAGATTCTT CCTACCCACC AACCCCCACC ACAGAGAGGA 
     1561 GTGTGGCAGT GGCAGTGAAA GACCAGAGGA AGGCCATCAA GACCCTGCTG TCATGGGTGC 
     1621 AGAGGAAAAC CAGGAAGTAT GGTGTGGCGG TCCAAGACTT CGCTGGCAGT TGGAGGAGTG 
     1681 GGCTAGCTTT CCTGGCTGTC ATCAAAGCTA TTGACCCCAG CCTCGTAGAC ATGAAGCAGG 
     1741 CTCTGGAAGA TTCTACCCGG GAAAATCTAG AGAAGGCATT CCGCATTGCA CATGATTCCC 
     1801 TGCATATCCC CAGGCTCCTC GAGCCGGAAG ACATCATGGT TGACATGCCG GATGAGCAGT 
     1861 CCATTGTGAC TTATGTGGCC CAGTTTCTAG AGCGCTTTCC GGAGTTGGAT CCCGAGGATT 
     1921 TTGTGAATCC AGACAAAGAA GCCCCTATCG AGTCCACCTT TGTCCGAATC AAAGAAAGCC 
     1981 CCTCTGAGCA AGAGAGCCGA GTCCTCCTCC TTAGCCAGAA TGGAGAGCAG CCCTACACTG 
     2041 TCAGCCATGA GACCAGCCAC CCACCGCCTG ACAAGGTCTT CGTCTGTGAC CAGCTTGAGA 
     2101 GCCCCACGGG CTTTAGTCTG GGTAGTGCTC CCAGCCACAA ACTGCCAGAC AGCCCCACAG 
     2161 ACTTTATGCA CCAGATTATT GACCAAGTCT TCCAGGCGAG CATGGGGAAC ACTGAATCCA 
     2221 CCACAGAGCC AACTCCAGAG TCATCCATCT TGTCAACCAG AAAGGATGGG AGGAGGTCCA 
     2281 ACTCTTTGCC TGTCAAGAAA AGCGTCCACT TCGAGACTGA CTTGCACAAG GATGCCTCGT 
     2341 GCAGCAAGGA CCCTTTCTAC AGCTCCGAGT TCCGCTTTGA GGGGAGCCCC AAGGCAACCA 
     2401 AGGAGCTGTC AAAGCAGGAT GGCCATGCCT CATTGGCTGA GGTTTCCAAG GAAAAGAAAC 
     2461 CAGAACAGGA AGCCAGGCTA GTGCTGGAAG CTGCCTCAGA CAAGGCCCCT GAAGATACTA 
     2521 TTGTTGACAC AGATGCTATG TCCCAAGATG CTCAGCTTCC CCAGGACTCC TCATTCTGTA 
     2581 ATGGCAATGT AGAGAATCCA GCCAGTCTAG GTGAGAAAGG CCCTTCGCCA CCATCCCCTA 
     2641 GAGATAATAC TATCCTGGCC AACTCCGTGG AGCTCAAGGT TCCGCTGCTG ACCGTTGAGC 
     2701 CTATGGACAA AGACGACTAC TTTGAATGTA TCCCGCTAAA AGCTTCCAAG TTTAACAGGG 
     2761 ACCTAGTAGA TTTCGCCTCC ACCAGCCAGG CTTTTGTTGA GGATCCTTCG TCCCACAAGA 
     2821 AGACACCTGG GGAGGAAGAG GGTTCTGAAA ATTACACCGA GAAACCAGGA AAAAGGAAAT 
     2881 CAAAGTCTCC CCATGCAGAA ACAGAGTCCA CCGAGTCCAG ATTGGAGCCC ATCAAGCTCG 
     2941 AGACTCCTCC TAAGGATCCT GAGCAAGAAG ACCAAGGCCA CACTTTGCCC CCAGAAACAG 
     3001 CCGCAGACAA AAAGCCCGAA GTGTATGAAA AGGCCAAGAG GAAATCCACT CGTCATCACA 
     3061 GCGAGGAAGA AGGAGAAGCA GAAGGCGGGC TCTCTGCAGT CGGCGGAGAA ATGCCATCCA 
     3121 ATCCTCCAAG TACCAGCGTC AGCCTGGAAA CCCTCAGAAG CCACAGCGAA GAAGGTTTGG 
     3181 ATTTCAAACC TTCGCCACCC CTCTCAAAGA TCTCCGTCAT CCCCCATGAC CTGTTCTACT 
     3241 ACCCACATTA CGAGGTGCCC CTGGCCGCAG TGCTAGAGGC CTATGCGGAA GGGGGCGAGG 
     3301 ATTTGAAAAG TGAAGATGCA GACCTAGAAC ATCCAGATGG GAGCTATCTG CAGGACTCCA 
     3361 GGGAGGAGGA GGAGGAGGAG GACGAGGAGG AGGAGGAGGC CCACAGCTCC CACAGCAGCT 
     3421 GCAATTTCTC CTTGCCTGTT GACAACAGCT ACCCCAGGGT CAATGACCAT GTGTCCCATG 
     3481 TTGACGGGAG TTCTGAGGGA CCCACATCAG CCTCGGAACC TGGTTCTCCG CCCTCCCATG 
     3541 AGGACCACCA GCCAATGGAA ACCAAAGAGA ATGGCCCTGT GGAAAGCCAG CAGTCCCAAG 
     3601 AACCCCCAAC CCCAGAACTG TCCACAAAGC CATTAGAAGA AAAGTTAACG GAAGTCCCAA 
     3661 CTAGCAGTAA AAAGAAGGAA AAAAGGAAAC ACATGGACCA CGTAGAAAGT TCATTATTTA 
     3721 TAGCTCCTGG GACGGTCCGC TCCTCAGATG ACCTTGAAGA AAACCCCTGC GAACACAAGG 
     3781 TCCCTTCCAG GAATAGTCAC AGTGACTCCA GCATTTACAT TCGGCGACAC ACTAATAGGT 
     3841 CTTTGGAATT GGACCATTTT AGCTATGTTC AATTGAGGAA CGCAGCCGAT GTGGATGACA 
     3901 GGAGAAACAG ACTGTTAAAC AGGACTGGCA CCAACTCCTC TGGAGAAGCC GTGCCCCTGG 
     3961 GGAGCCAGAG CCCGCCCAAC GACTCTCTGA CGCAGTTTGT ACAGCAGCCC GACGTGATAT 
     4021 ATTTCATTCT CTTCCTCTGG CTCCTGGTTT ACTGCTTACT ACTCTTCCCC CAGCTCGATG 
     4081 TCAGCCGGCT CACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4141 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4201 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4261 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4321 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4381 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4441 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4501 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4561 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4621 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     4681 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     4741 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     4801 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     4861 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     4921 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     4981 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5041 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5101 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5161 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5221 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5281 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5341 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5401 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5461 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5521 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     5581 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     5641 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     5701 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     5761 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     5821 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     5881 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     5941 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6001 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6061 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6121 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6181 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6241 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6301 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6361 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6421 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6481 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6541 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     6601 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     6661 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     6721 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     6781 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     6841 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     6901 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     6961 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7021 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7081 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7141 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7201 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7261 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7321 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7381 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7441 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7501 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7561 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     7621 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     7681 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     7741 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     7801 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     7861 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     7921 TAACAAAAAT TTAACGCGAA TTTT
//