LOCUS       RR208800                8856 bp    DNA     circular  SYN 
DEFINITION  Usp42 (Myc-DDK-tagged ORF) - Rat ubiquitin specific peptidase 42 (Usp42), (10 ug)
ACCESSION   NM_001105909
VERSION     NM_001105909.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5013..5104 
                     /label="Myc-DDK"  
     misc_feature    5134..5719 
                     /label="PolyA signal"  
     misc_feature    5191..5210 
                     /label="Restriction Enzyme"  
     misc_feature    5995..6667 
                     /label="ColE1"  
     misc_feature    7097..7891 
                     /label="Kan/Neo"  
     misc_feature    7926..8228 
                     /label="SV40 ori"  
     misc_feature    8314..8791 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4995..5001 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4995 
                     /label="Usp42"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACCATAGTT GACAAAACTG AACCTTCAGA CCCATCAACC TGTCAGAACC 
     1081 AGCCTGGCAG TTCTGAGGCG GTCTCACCTG AAGACATGGA CACAGGCTCT GCCAGCTGGG 
     1141 GTGCCGTGTC TTCCATAAGT GATGTCTCAA ATCATACACT TTCATTGGGG CCGGTGCCTG 
     1201 GTGCTGTAGT TTACAATAAC TCGTCTGTAC CTGAGAAATC AAAACCATCA CCACCAAAGG 
     1261 ATCAAGTCCT AGGTGATGGC ATTGCTCCTC CTCAAAAGGT CCTATTTCCA TCTGAAAAGA 
     1321 TTTGTCTTAA GTGGCAACAA AGTCATCGAG TTGGCGCTGG GCTGCAGAAT TTGGGCAATA 
     1381 CCTGTTTTGC CAATGCTGCG TTGCAATGTC TGACTTACAC ACCACCCCTC GCCAACTACA 
     1441 TGCTATCCCA TGAACACTCC AAGACATGCC ATGCAGAAGG ATTCTGTATG ATGTGCACAA 
     1501 TGCAGACGCA CATTACCCAG GCACTTAGCA ACCCTGGGGA TGTTATCAAG CCCATGTTCG 
     1561 TCATCAATGA AATGCGGCGT ATAGCTAGAC ATTTCCGTTT TGGTAACCAA GAAGATGCCC 
     1621 ATGAATTTCT TCAGTACACG GTCGATGCCA TGCAGAAAGC ATGTTTAAAT GGCAGCAATA 
     1681 AATTAGACAG ACATACCCAG GCCACCACCC TGGTCTGCCA GATATTTGGA GGCTACCTAA 
     1741 GATCCCGAGT CAAGTGTTTA AATTGCAAGG GTGTTTCAGA CACCTTTGAT CCATACCTGG 
     1801 ACATAACACT GGAGATTAAG GCTGCACAGA GCGTTACCAA GGCGCTGGAA CAGTTCGTGA 
     1861 AGCCAGAACA ACTGGATGGA GAAAACTCCT ACAAATGTAG CAAGTGCAAA AAGATGGTTC 
     1921 CAGCTTCAAA GAGATTCACC ATCCATAGGT CCTCTAATGT TCTTACCATT TCACTGAAAC 
     1981 GGTTTGCCAA CTTTACCGGT GGAAAAATTG CTAAGGACGT GAAATATCCC GAGTACCTTG 
     2041 ATATCCGGCC CTATATGTCT CAGCCCAACG GAGAGCCCAT CATTTATGTT TTGTATGCTG 
     2101 TGCTGGTGCA CACCGGTTTT AACTGTCATG CTGGCCACTA CTTTTGCTAC ATCAAGGCTA 
     2161 GCAATGGCCT CTGGTATCAG ATGAATGACT CTATCGTGTC CACCAGTGAT ATTAGATCGG 
     2221 TGCTTAACCA GCAGGCTTAC GTGCTCTTTT ATATCAGGTC CCATGATGTG AAAAATGGAG 
     2281 GGGAGACCAC TCATCCTGCC CATAGCCCTG GCCAGTCCTC CCCCCGGCCA GGGATCAGTC 
     2341 AGCGTGTAGT CAACAGCAAG CAGGCGGCGC CAGGATTCAT CGGGCCGCAG CTGCCTCCCC 
     2401 ATGTGATGAA GAACACACCA CACTTGAATG GCACCACGCC GGTGAAAGAC ACACCAAGCA 
     2461 GTTCTGTTTC AAGCCCTAAC GGAAACACCA GCGTCAATAG GGCCAGTCCT GCTACGGCTT 
     2521 CGACGTCTGT GCAGAACTGG TCTGTTAACA GGCCCTCAGT TATTCCGGAA CACCCTAAGA 
     2581 AACAAAAAAT TACTATCAGT ATTCACAACA AGTTGCCTGT TCGTCAAGGT CAGGCACAGC 
     2641 TGAATAACAA CCTCCATGGC CCTTGTCTGG AGGCCCCTAG CAAGGCGGCC CCCTCCTCCA 
     2701 CCATCACTAA CCCTTCTGCA ATACAGTCTA CCTCGAATGC ACCCACGACA TCAGTTTCCC 
     2761 CAAGTGAGTC CTGTCCCAAG CCCATGGTGA ATGGCAAGGC TAAAGTGGGC TCCAGTGTGC 
     2821 TCGTCCCCTA TGGGGCTGAG TCCTCAGAGG AGTCTGATGA GGAGTCCAAG GGCCTGGCCA 
     2881 AGGAGAACGG TGTGGACATG ATGACCAGCA CTCAGTCCAG TAGACCAGAA ACCGCAGATG 
     2941 AGGACGGTGA GGCTTCCCCT CATGAGCTTC AAGAGTCCGT CATGCTAAAT GGTGCTAATA 
     3001 GCGCAGACAG TGACTCGAGA GAGAGCAGCC TGGCATTTGA CAGTGCCAGC TGCCAGGTCC 
     3061 AGCCCGATCT ACACACAGAA AACCTCTTTA ACAAACTCAA TGGTCTTCCT GGAAAGGTGA 
     3121 CGCCCGCTCC ATTGCAGTCT GTTCCTGAAG ACAAAATCCT TGAGACTTTC AAGCTTACCA 
     3181 ACCAGGCAAA AGGTCCAGCG GTTGAAGAGA GTTGGACTAC AACAGGGGGA AGCTCTCCAA 
     3241 GGGCCTCTGT TTCACAGCCG GAGCCCATCA CCGATGAGAC CAGTCCCCTT GAGATACCGG 
     3301 AGGCAGTCAC CAACGGGAGC ATGAAGACCC CTTCCACCAC GTCACCTCTG GAGCCCACCA 
     3361 TCAGCTGTAC CAAAGAAGAC TCTTCCGTCG TCGTCGTCTC AGCAGAACCT ATGGAGGTTT 
     3421 TGCCTTCCGT CCCTGCTCTC TGTAACAGCA CCGGTACTAC CTTGGGGGAT GCCCCAGTGC 
     3481 CCGAATCGTG TGACCCTGGA GACTTGACTG CCAACCCGAG CCAACCATCG CAAGCGGTGA 
     3541 AGGGTGATCC AGCTGAGAAC TCTGAGAACT CCACCTTGGC TGAAGTGGTG GAGAGGCTGA 
     3601 GCCCTGCTCC CTCTGTGCTC ACAGGGGACG GGTGTGAGCA GAAACTCTTC CTTTACCTCA 
     3661 GTACAGAGGG GTCCGAGGAG GCAGAAGACT CCTCCAGGAG CTCGGCAGTC TCTGCTGACA 
     3721 CGGTGCCCCC TCAGCCTGAC AGGACCACCA GCTCCTGTGC AGGGGCTGCC GAGCAGGCTG 
     3781 CCGGGGATAG AGGCGATGGA GGCAGTGTGG GACCCAAAGC TCAGGAGCCC TCCCCGGCCA 
     3841 AGGAAAAGAT GAACAGCCTT CGGAAAGTGG ATCGAGGACA CTATCGGAGC CGGAGAGACC 
     3901 GGTCCTCCAG TGGGGAGCAC GTGAGGGACA GCAGGGCCCG ACCAGAGGAC CATCACCATA 
     3961 AGAAGCGGCG CTGCTACAGC CGAGAGCGGC CCAAGCAGGA GCGCCACCCT GCTAACACAT 
     4021 ACTGCAATGG GGGCCAGCAC CTGGGCCACG GCGACAGAGC CAGCCCCGAG CGCCGCTCCC 
     4081 TGAGCAGGTA CAGCCACCAT CACTCACGGG TTAGGAGTGG CCTGGAGCAG GACTGGAGCC 
     4141 GGTACCACCA CTCGGAAAAT GAGCCTGCCT GGGCCAGGGA GAGGTTCTAC CCGGACAAGA 
     4201 TGCGCTGGGA CAAGTGTAGG TATTACCACG ACAGGTACAC TACGACCCTG TACACGGCCC 
     4261 GGGAGGCAGC CCGCGAGTGG CGGCCTCTAC ATGGTCGTGA GCATGAACGC CTTGTCCAGT 
     4321 CTGGACGGCC ACACAAGGAC AGCTACTGGG GCCGCAAGGG CTGGGACCTA CAATCCCGGG 
     4381 GGAAGGAACG GCCCCACTTC CATAGCCCAC GAGAGGCCCC TGGCCTTGCT GTACCCCTCG 
     4441 AGAGGCATCT CCAAGAGAAG GCTGCGCTGA GTGTGCAGGA CAGCAGCCAC AGTCTCCCTG 
     4501 AGCGCTTTCA TGAACACGAA AGTGTCAAGT TGAGGAAAAG GAGGTATGAG ACTCTAGACA 
     4561 ATAATGACGA CCATCCAGAA AAGAAAGTCC ACAAAAGCCT GGAGAAGGAC GCGCTAGAAG 
     4621 AGCCAAGGGC AAAGAAGCAC AAAAAATCTA AAAAGAAAAA GAAGTCCAAA GATAAACACC 
     4681 GGGCTCGGGA TAGCAGGCAC CAGCAAGAAT CCGACTTTTC AGGAGCATAC TCTGATGCTG 
     4741 ACCTCCATAG ACACCGGAAG AAAAAGAAGA AAAAGAAAAG GCATTCAAGG AAGTCGGAGG 
     4801 ACTTTATAAA AGATGTTGAG ATGCATTTAC CGAAGCTCTC GAGCTATGAG GCCGTTGGCC 
     4861 ATTTCCGGAG AACAGAGGGC ACCTTTCTAC TGGCTGATGG TCTGCCTGTG GAAGACAGCG 
     4921 GCCCTTTCCG GGAGAAAACG AAGCATTTAA GGATGGAAAG CCGGCCTGAC AGATGTCATC 
     4981 TGTCAGAGTA TGGCCAGGGT AACACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     5041 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     5101 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     5161 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     5221 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     5281 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     5341 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     5401 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     5461 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5521 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5581 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5641 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5701 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5761 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5821 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5881 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5941 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     6001 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     6061 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     6121 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     6181 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     6241 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     6301 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     6361 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     6421 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     6481 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6541 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6601 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6661 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6721 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6781 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6841 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6901 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6961 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     7021 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     7081 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     7141 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     7201 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     7261 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     7321 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     7381 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     7441 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     7501 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7561 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7621 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7681 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7741 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7801 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7861 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7921 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7981 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     8041 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     8101 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8161 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8221 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     8281 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     8341 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     8401 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     8461 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     8521 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     8581 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8641 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8701 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8761 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8821 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//