LOCUS       RR208788                8127 bp    DNA     circular  SYN 
DEFINITION  Usp28 (Myc-DDK-tagged ORF) - Rat ubiquitin specific peptidase 28 (Usp28), (10 ug)
ACCESSION   NM_001108144
VERSION     NM_001108144.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4282..4373 
                     /label="Myc-DDK"  
     misc_feature    4403..4988 
                     /label="PolyA signal"  
     misc_feature    4460..4479 
                     /label="Restriction Enzyme"  
     misc_feature    5264..5936 
                     /label="ColE1"  
     misc_feature    6366..7160 
                     /label="Kan/Neo"  
     misc_feature    7195..7497 
                     /label="SV40 ori"  
     misc_feature    7583..8060 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4257..4264 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4257 
                     /label="Usp28"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACTGCGGAG CTGCAGCAGG ACGACGCGGC GGGGGCGGCG GACGGCCATG 
     1081 GCTCGAGCTG CCAGATGCTG TTAAATCAGC TGCGAGAAAT CACGGGCATC CAGGATCCTT 
     1141 CCTTTCTTCA TGAAGCATTA AAGGCCAGTA ATGGTGACAT CACCCAGGCT GTCAGCCTTC 
     1201 TCACAGACCA AAGAGTTAAG GAGCCCAGTC ACGACACAGC TGCTACAGAA CCATCTGAAG 
     1261 TAGAAGAGAG TGCTACCAGC AAAGATCTCT TAGCAAAAGT GATAGACCTT ACACATGACA 
     1321 ACAAAGATGA CCTTCAGGCA GCCATTGCCT TGAGTCTCCT GGAGTCTCCT AACATTCAGA 
     1381 CTGACAGCAG AGATCTTAAT AGAATACATG AAGCTAACTC TGCAGAGACT AAACGATCAA 
     1441 AGAGAAAACG CTGTGAAGTT TGGGGAGAAA ACCACAATCC CAATAACTGG AGGAGAGTGG 
     1501 ATGGTTGGCC AGTTGGGCTG AAAAATGTTG GCAACACATG TTGGTTCAGT GCTGTTATTC 
     1561 AGTTGCCTGA ATTTCGAAGA CTTGTTCTCA GTTACAGCCT GCCACAGAAC ATTCTTGAAA 
     1621 ATTGTCGAAG TCACACTGAA AAAAGAAATA TCATGTTTAT GCAAGAGCTT CAGTATTTAT 
     1681 TTGCTCTGTT ATTGGGATCA AATCGGAAAT TTGTAGATCC TTCTGCTGCC CTGGATCTAC 
     1741 TGAAGGGGGC CTTCCGATCC TCAGAGGAGC AACAGCAAGA TGTGAGTGAA TTCACACACA 
     1801 AGCTCCTGGA TTGGCTGGAG GATGCGTTCC AGCTAGCTGT TAATGTTAAC AGCAATCCCA 
     1861 GGACTAAATC TGAAAACCCC ATGGTGCAGC TGTTCTACGG TACCTTCCTG ACAGAAGGGG 
     1921 TCCGAGAAGG AAAACCCTTT TGTAACAACG AGACCTTCGG CCAGTACCCT CTTCAGGTAA 
     1981 ACGGTTACCA CAACTTAGAC GAGTGTTTGG AAGGGGCCAT GGTGGAAGGT GACATTGCGC 
     2041 TGCTTCCTTC TGATCGCTCA GTGAAATACG GACAAGAGCG TTGGTTTACA AAGCTGCCTC 
     2101 CAGTGTTGAC CTTCGAACTC TCCAGATTTG AGTTTAATCA GTCTCTTGGG CAGCCTGAGA 
     2161 AAATTCATAA TAAGCTGGAA TTTCCTCAGG TTATTTATAT GGATAGGTAC ATGTACAAAA 
     2221 GCAAAGAGCT TATCCGAAGT AAGAGAGAGA GCATTCGAAA GTTGAAGGAG GAAATACAAG 
     2281 TTCTGCAGCA GAAATTAGAA AGGTATGTGA AGTACGGCTC CGGTCCATCT CGCTTCCCAC 
     2341 TCCCCGACAT GTTGAAATAC GTTATTGAAT TTGCCAGTAC AAAACCTGCC TCTGAAAGCT 
     2401 GTCTGTCTGG AAGTGTTGAG CATATGACGT TACCACTTCC TTCTGTGCAC TGCCCGATTT 
     2461 CTGACCTCAC AGCCAAGGAA AGTTCAAGTC CCAAAAGCTG CTCTCAGAAT GCTGAGGGTT 
     2521 CCTTTTCCTC TCCGGAAGAT GCTCTACCCA ACTCTGAGGT AATGAATGGG CCATTTACTT 
     2581 CTCCTCACTC TTCCCTGGAA ATGCCTGCAC CCCCACCAGC TCCTCGGACA GTCACAGATG 
     2641 AGGAAATGAA TTTCGTTAAG ACCTGTCTTC AGAGGTGGCG GAGTGAAATT GAACAGGATA 
     2701 TACAAGACTT AAAGAATTGT ATCTCGAGCA CCACCCAGGC TATTGAGCAG ATGTACTGTG 
     2761 ATCCTCTTCT TCGTCAGGTG CCTTATCGCT TACATGCAGT TCTTGTTCAT GAAGGACAAG 
     2821 CCAGTGCAGG GCACTACTGG GCGTACATCT ATAACCAGCC CCGGCAGATC TGGCTCAAAT 
     2881 ACAACGACAT CTCTGTCACT GAGTCTTCCT GGGAAGAACT TGAAAGAGAT TCTTACGGGG 
     2941 GCCTGAGAAA TGTCAGCGCA TACTGCTTGA TGTATATCAA TGACAAGTTG CCCCACTGCA 
     3001 GTGCAGAGGC AGCGCATGGT GAATCTGATC AGACTGCAGA AGTGGAAGCC CTGTCTGTGG 
     3061 AACTCAGGCA GTACATTCAG GAAGATAACT GGAGGTTTGA GCAAGAGGTG GAGGAATGGG 
     3121 AGGAAGAACA GTCCTGTAAG ATCCCACAGA TGGAGTCCTC CCCAAACTCT TCCTCACAGG 
     3181 ATTTTTCCAC ATCACAAGAG TCTTCAGCAG CGTCCTCGCA TGGCGTTCGA TGTTTGTCTT 
     3241 CTGAACATGC TGTGATTGCA AAGGAGCAGA CTGCCCAGGC CATCGCAAAC ACGGCTCATG 
     3301 CCTATGAGAA GAGCGGGGTA GAAGCAGCCT TGAGTGAGGT GATGCTGAGC CCTGCCATGC 
     3361 AAGGGGTCCT CCTGGCCATA GCTAAGGCGC GCCAGACCTT TGACCGAGAC GGGTCTGAAG 
     3421 CAGGGCTTAT TAAGGCATTT CATGAAGAGT ACTCCAGGCT CTATCAGCTT GCCAAAGAGA 
     3481 CACCCACCTC TCACAGTGAT CCTCGCCTTC AGCATGTTCT CGTCTACTTT TTCCAAAATG 
     3541 AAGCACCCAA GAGGGTAGTA GAACGAACCC TTCTGGAACA GTTTGCAGAT AGAAACCTTA 
     3601 GCTATGATGA GAGATCTATC AGTATCATGA AGGTGGCTCA GGCCAAACTG ATGGAAATTG 
     3661 GTCCAGAGGA CATGAACATG GAAGAGTATA AGAGGTGGCA TGAAGACTAT AGTCTGTTCC 
     3721 GAAAGGTGTC TGTGTACCTG CTAACGGGCC TGGAACTCTT CCAAAAAGGA AAGTACCAGG 
     3781 AAGCTTTGTC CTACCTGGTG TACGCGTACC AGAGCAATGC TGGCCTGCTG GTGAAGGGGC 
     3841 CCCGCAGGGG CGTGAAGGAG TCTGTGATTG CTTTGTACCG CAGAAAATGC CTTCTGGAGT 
     3901 TGAATGCCAA GGCTGCTTCT CTTTTTGAAA CAAATGACGA CCATTCTGTA ACAGAGGGCA 
     3961 TTAATGTGAT GAATGAGCTG ATCATTCCCT GCATCCACCT GATCATTAAT AATGACATTT 
     4021 CCAAGGACGA CCTTGATGCC ATCGAGGTCA TGAGGAACCA CTGGTGCTCT TACCTGGGGA 
     4081 AGGACATCGC AGAAAATCTG CAGCTGTGTT TAGGGGAGTT CCTACCCAGG CTTCTAGATC 
     4141 CTTCCGCAGA AATCATCGTC CTAAAGGAGC CTCCAACTAT TCGACCGAAT TCTCCCTATG 
     4201 ATCTTTGCAG CCGGTTTGCA GCTGTCATGG AGTCCATTCA AGGAGTTTCA ACAGTAACAG 
     4261 TGAAGAGCGG ACCGACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4321 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     4381 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4441 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4501 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4561 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4621 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4681 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     4741 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     4801 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     4861 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     4921 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     4981 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5041 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     5101 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5161 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5221 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     5281 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     5341 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     5401 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     5461 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     5521 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     5581 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     5641 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     5701 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     5761 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     5821 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     5881 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     5941 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6001 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6061 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6121 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6181 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6241 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6301 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     6361 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6421 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     6481 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     6541 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     6601 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     6661 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     6721 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     6781 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     6841 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     6901 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     6961 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7021 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7081 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7141 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7201 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7261 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7321 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     7381 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7441 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7501 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     7561 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     7621 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     7681 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     7741 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     7801 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     7861 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     7921 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     7981 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     8041 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     8101 ATTTAACAAA AATTTAACGC GAATTTT
//