LOCUS       RR208760                7977 bp    DNA     circular  SYN 
DEFINITION  Tlr9 (Myc-DDK-tagged ORF) - Rat toll-like receptor 9 (Tlr9), (10 ug)
ACCESSION   NM_198131
VERSION     NM_198131.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4134..4225 
                     /label="Myc-DDK"  
     misc_feature    4255..4840 
                     /label="PolyA signal"  
     misc_feature    4312..4331 
                     /label="Restriction Enzyme"  
     misc_feature    5116..5788 
                     /label="ColE1"  
     misc_feature    6218..7012 
                     /label="Kan/Neo"  
     misc_feature    7047..7349 
                     /label="SV40 ori"  
     misc_feature    7435..7912 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4116..4122 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4116 
                     /label="Tlr9"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTTCTCTGT AGCAGGACCC TGCACCCCTT GTCTCTCCTG GTACAGGCTG 
     1081 CAGTGCTGGC TGAGGCTCTG GCCCTGGGTA CCCTGCCTGC CTTCCTACCC TGTGAACTGA 
     1141 AGCCTCATGG CCTGGTAGAC TGCAACTGGC TGTTCCTGAA GTCTGTGCCT CACTTCTCTG 
     1201 CCGCAGAACC CCGTTCCAAC ATCACCAGCC TTTCCTTGAT CGCCAACCGC ATCCACCACC 
     1261 TGCACAACCT CGACTTTGTC CACCTGCCCA ACGTGCGACA GCTGAACCTC AAGTGGAACT 
     1321 GTCCGCCCCC TGGTCTCAGC CCCTTGCACT TCTCCTGCCG CATGACCATT GAGCCCAAAA 
     1381 CCTTCCTGGC TATGCGCATG CTGGAAGAGC TGAACCTGAG CTATAACGGT ATCACCACTG 
     1441 TGCCCCGCCT GCCCAGCTCC CTGACGAATC TGAGCCTAAG CCACACCAAC ATCCTGGTAC 
     1501 TCGATGCCAG CAGCCTTGCT GGCCTGCACA GCCTGCGAGT TCTCTTCATG GACGGGAACT 
     1561 GCTACTACAA GAACCCCTGC AACGGGGCGG TGAACGTGAC CCCGGACGCC TTCCTGGGCT 
     1621 TGAGCAACCT CACCCACTTG TCCCTTAAGT ATAACAACCT CACAGAGGTG CCCCGCCAAC 
     1681 TGCCCCCCAG CCTGGAGTAC CTCCTGCTGT CCTATAACCT CATCGTCAAG CTGGGGCCCG 
     1741 AAGACCTAGC CAACCTGACC TCCCTTCGAG TGCTTGATGT GGGTGGGAAT TGCCGTCGCT 
     1801 GTGATCACGC CCCCGACCTC TGTACAGAAT GCCGGCAGAA GTCCCTTGAT CTGCACCCTC 
     1861 AGACTTTCCA TCACCTGAGC CACCTTGAAG GCCTGGTGCT GAAGGACAGT TCTCTCCACT 
     1921 CGCTGAACTC CAAGTGGTTC CAGGGTCTGG TGAACCTCTC GGTGCTGGAC CTAAGCGAGA 
     1981 ACTTTCTCTA CGAGAGCATC AACAAAACCA GCGCCTTTCA GAACCTGACC CGTCTGCGCA 
     2041 AGCTCGACCT GTCCTTCAAT TACTGCAAGA AGGTATCGTT CGCCCGCCTC CACCTGGCAA 
     2101 GTTCCTTCAA GAGCCTGGTG TCGCTGCAGG AGCTGAACAT GAACGGCATC TTCTTCCGCT 
     2161 TGCTCAACAA GAACACGCTC AGGTGGCTGG CTGGTCTGCC CAAGCTCCAC ACGCTGCACC 
     2221 TTCAAATGAA TTTCATCAAC CAGGCGCAGC TCAGCGTCTT TAGTACCTTC CGAGCCCTTC 
     2281 GCTTTGTGGA CCTGTCCAAT AATCGCATCA GCGGGCCTCC AACGCTGTCC AGAGTCGCCC 
     2341 CCGAAAAGGC AGACGAGGCG GAGAAGGGGG TTCCATGGCC TGCAAGTCTC ACCCCAGCTC 
     2401 TCCCGAGCAC TCCCGTCTCA AAGAACTTCA TGGTCAGGTG TAAGAACCTC AGATTCACCA 
     2461 TGGACCTGTC TCGGAACAAC CTGGTGACTA TCAAGCCAGA GATGTTCGTC AACCTCTCCC 
     2521 ATCTCCAGTG TCTGAGCCTG AGCCACAACT GCATCGCGCA GGCTGTCAAT GGCTCTCAGT 
     2581 TCCTGCCGCT GACCAACCTG AAGGTGCTGG ACCTGTCCTA TAACAAGCTG GACCTGTACC 
     2641 ATTCGAAATC GTTCAGTGAG CTCCCACAGT TGCAGGCCCT GGACCTGAGC TACAACAGCC 
     2701 AGCCCTTCAG CATGCAGGGG ATAGGCCACA ACTTCAGTTT TCTGGCCAAT CTGTCCAGGT 
     2761 TACAGAACCT TAGCCTGGCA CACAATGACA TTCACAGCCG CGTGTCCTCA CGCCTCTACA 
     2821 GCACCTCAGT GGAGTATCTG GACTTCAGCG GCAACGGTGT GGGCCGCATG TGGGACGAGG 
     2881 AGGACCTTTA CCTCTATTTC TTCCAAGACC TGAGAAGCCT GATTCATCTG GACCTATCTC 
     2941 AGAATAAGCT GCACATCCTC CGGCCCCAGA ACCTCAACTA CCTCCCCAAG AGCCTGACGA 
     3001 AGCTGAGTTT CCGTGACAAT CACCTCTCTT TCTTTAACTG GAGCAGTCTG GCCTTCCTGC 
     3061 CCAATCTGCG AGACCTGGAC CTGGCAGGCA ATCTACTAAA GGCCCTGACC AACGGCACCC 
     3121 TGCCTAATGG CACGCTCCTC CAGAAACTGG ATGTCAGTAG CAACAGTATC GTCTTTGTGG 
     3181 TCCCAGCCTT CTTTGCTCTG GCGGTAGAGC TAAAAGAGGT CAACCTCAGC CATAACATCC 
     3241 TCAAGACTGT GGATCGCTCC TGGTTTGGGC CCATTGTGAT GAACCTGACG GTTCTAGACG 
     3301 TGAGCAGCAA CCCTCTGCAT TGTGCCTGCG GTGCACCCTT TGTAGACTTA CTGCTGGAAG 
     3361 TGCAGACCAA GGTGCCTGGC CTGGCTAACG GTGTGAAGTG TGGCAGTCCC CGCCAGCTGC 
     3421 AGGGCCGCAG CATCTTTGCG CAAGACCTGC GGCTGTGCCT GGATGACGTC CTTTCTCGGG 
     3481 ACTGCTTTGG CCTTTCACTC CTGGCTGTGG CCGTGGGCAC GGTGTTGCCT TTACTGCAGC 
     3541 ATCTCTGCGG CTGGGACGTC TGGTACTGTT TCCATCTGTG CCTGGCATGG CTACCTTTGC 
     3601 TGACCCGTGG CCGGCGCAGC GCCCAAGCTC TCCCTTATGA TGCCTTCGTG GTGTTCGATA 
     3661 AGGCGCAGAG CGCGGTTGCT GACTGGGTGT ATAACGAGCT TCGAGTGCGG CTAGAGGAGC 
     3721 GGCGCGGTCG CCGAGCCCTA CGCTTGTGTC TGGAGGACCG AGATTGGCTG CCTGGCCAGA 
     3781 CACTCTTCGA GAACCTCTGG GCCTCCATCT ATGGCAGCCG CAAGACTCTG TTTGTGCTGG 
     3841 CCCACACGGA CAAGGTCAGT GGCCTCCTGC GCACCAGCTT CCTGCTGGCT CAGCAGCGCC 
     3901 TGCTGGAGGA CCGCAAGGAC GTGGTGGTGT TGGTGATCCT GCGCCCTGAT GCCCACCGCT 
     3961 CCCGCTACGT GCGACTGCGC CAGCGCCTCT GCCGCCAGAG TGTGCTCTTC TGGCCCCATC 
     4021 AGCCCAACGG GCAGGGCAGC TTCTGGGCCC AGCTGAGTAC AGCCCTGACT AGGGACAACC 
     4081 ACCACTTCTA TAACCGGAAC TTCTGCCGGG GACCTACAGC AGAAACGCGT ACGCGGCCGC 
     4141 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4201 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4261 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4321 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4381 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4441 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4501 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4561 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4621 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4681 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     4741 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     4801 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     4861 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     4921 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     4981 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5041 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5101 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5161 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5221 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5281 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5341 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5401 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5461 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     5521 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     5581 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     5641 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     5701 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     5761 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     5821 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     5881 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     5941 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6001 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6061 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6121 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6181 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6241 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6301 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6361 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6421 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6481 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     6541 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     6601 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     6661 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     6721 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     6781 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     6841 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     6901 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     6961 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7021 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7081 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7141 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7201 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7261 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7321 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7381 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7441 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7501 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     7561 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     7621 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     7681 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     7741 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     7801 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     7861 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     7921 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//