LOCUS       RR208691                8397 bp    DNA     circular  SYN 
DEFINITION  Mcf2l (Myc-DDK-tagged ORF) - Rat MCF.2 cell line derived transforming sequence-like (Mcf2l), (10 ug)
ACCESSION   NM_053951
VERSION     NM_053951.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4554..4645 
                     /label="Myc-DDK"  
     misc_feature    4675..5260 
                     /label="PolyA signal"  
     misc_feature    4732..4751 
                     /label="Restriction Enzyme"  
     misc_feature    5536..6208 
                     /label="ColE1"  
     misc_feature    6638..7432 
                     /label="Kan/Neo"  
     misc_feature    7467..7769 
                     /label="SV40 ori"  
     misc_feature    7855..8332 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4536..4542 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4536 
                     /label="Mcf2l"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGGTTTTGG CTGAGAAATG AAGAGATGGC CCTAGAAGAA ATGGTGCAGA 
     1081 GATTAAACGC GGTTTCCAAG AACACTGATG AAATCATGCA CCAGGACATC GTCCCACTCT 
     1141 GTGCCGCTGA CATCCAGGAG CAGCTGAAGA AGCGCTTTGC TTACCTGTCT GGTGGGCGGG 
     1201 GACAGGACGG AAGCCCAGTC ATCACCTTCC CAGACTACCC AGCCTTCAGC GAGATCCCGG 
     1261 ACAAGGAGTT CCAGAATGTC ATGACCTATC TCACCAGCAT CCCCAGTTTG CAAGATGCTG 
     1321 GCATCGGGTT CATCTTGGTC ATAGACCGAA GACAGGACAA ATGGACTTCT GTGAAGGCGT 
     1381 CAGTCCTGCG AATAGCCGCA TCATTCCCAG CCAACCTGCA GCTCGTCCTC GTTCTCCGAC 
     1441 CCACGGGCTT CTTCCAGAGG ACTCTCTCGG ACATAGCCTT CAAGTTCAAC AGAGATGAGT 
     1501 TTAAGATGAA AGTACCGGTC ATGATGCTAA GCTCAGTGCC AGAACTACAT GGCTACATTG 
     1561 ACAAGTCACA GCTGACCGAG GACCTTGGGG GGACCCTGGA CTACTGCCAC TCCAGGTGGC 
     1621 TGTGCCACCG CACAGCGATT GAAAGCTTCG CCCTGATGGT GAAGCAGACA GCCCAGATGC 
     1681 TGCAGGCCTT TGGGACCGAG CTGGCCGAGA CTGAGCTGCC CAACGACGTC CAGTCAACCA 
     1741 GCCTGGTGCT CAGCGCACAC ACAGAGAAGA AGGCTAAAGT GAAGGAGGAT CTGCAGCTGG 
     1801 CGCTGACGGA GGGGAACAGC ATCCTTGAGA GCCTCAGGGA GCCGCTGGCT GAGAGCATAG 
     1861 TCCACAGTGT GAACCAGGAC CAGCTGGACA ATCAGGCCAC TGTGAAGAGG CTCCTGACCC 
     1921 AGCTGAATGA GACCGAGGCT GCCTTTGACG AGTTCTGGGC AAAGCATCAG CAGAAGCTGG 
     1981 AACAGTGCCT ACAGCTGCGA CATTTTGAAC AAGGTTTCCG GGAGGTCAAA ACCGCCTTGG 
     2041 ATTCCATGTC CCAGAAGATA GCCGCGTTCA CAGATGTTGG CAACAGCCTG GCCCATGTGC 
     2101 AGCACCTCCT GAAGGACTTG ACCACCTTTG AGGAGAAGTC CAGCGTGGCC GTGGACAAGG 
     2161 CCAGAGCCCT GTCCCTGGAG GGGCAGCAGC TGATTGAGAA CCGGCACTAT GCTGTAGACT 
     2221 CTATCCATCC GAAGTGTGAG GAACTCCAGC ACCTCTGTGA CCACTTCGCC TCTGAGGTCA 
     2281 CCAGGAGGCG GGACCTTCTC AGCAAGTCCT TGGAGCTGCA TAGCCTCCTG GAGACGTCCA 
     2341 TGAAGTGGAG CGACGAGGGC ATCTTCCTGC TGGCCTCTCA GCCTGTGGAC AAATGCCAAT 
     2401 CTCAGGATGG TGCGGAGGCA GCCCTGCAGG AGATCGAGAA GTTTCTGGAG ACCGGTGCAG 
     2461 AAAACAAGAT TCAGGAGCTC AATAAGATTT ACAAGGAATA TGAATGCATC CTCAACCAGG 
     2521 ACCTTCTGGA ACACGTGCAA AAAGTCTTTC AGAAGCAAGA GAGCACCGAG GAGATGTTCC 
     2581 ACCGCAGGCA GGCTAGCCTG AAGAAACTGG CAGCCAAGCA GACACGGCCT GTGCAGCCCG 
     2641 TGGCCCCCCG ACCAGAGGCG CTAACCAAGT CCCCCTCCCC CTCGCCAGGC TCCTGGCGAA 
     2701 GCTCTGAGAA CTCCAGCTCT GAGGGCAACG CCCTCCGCAG AGGGCCCTAC AGGAGAGCCA 
     2761 AGAGCGAAAT GAGTGAGCCC CGGCAGGGCC GCACCAGCTC CACAGGAGAA GAGGAGGAGA 
     2821 GCCTGGCCAT CCTGCGCAGA CACGTGATGA ATGAACTTCT GGACACGGAG CGGGCCTACG 
     2881 TGGAGGAGCT GCTCTGTGTC TTAGAGGGCT ATGCTGCAGA GATGGACAAC CCCTTGATGG 
     2941 CACACCTCAT CTCAACAGGC CTGCAAAACA AGAAGAACAT TCTGTTTGGA AACATGGAGG 
     3001 AAATCTATCA CTTCCACAAC AGAATATTCC TGCGGGAGCT GGAGAGCTGC ATCGACTGCC 
     3061 CAGAGCTGGT GGGGAGATGC TTTCTGGAGA GGATGGAGGA GTTCCAGATC TATGAGAAGT 
     3121 ACTGTCAGAA CAAGCCACGC TCCGAGAGCC TGTGGAGACA GTGCTCTGAC TGTCCCTTCT 
     3181 TCCAGGAGTG CCAGAAGAAG CTGGACCACA AGCTGAGCCT CGACTCCTAC CTGCTGAAGC 
     3241 CTGTGCAGAG GATAACCAAG TACCAGCTGC TGCTCAAGGA AATGCTGAAG TACAGCAAGC 
     3301 ACTGTGAAGG GGCCGAGGAC CTGCAAGAGG CACTGAGCTC CATCCTGGGC ATCCTCAAGG 
     3361 CAGTGAACGA CTCCATGCAC CTCATAGCCA TCACTGGCTA TGATGGAAAC CTTGGGGACC 
     3421 TGGGGAAGCT GCTGATGCAA GGCTCCTTCA GCGTGTGGAC GGACCACAAG AAGGGCCACA 
     3481 CCAAGGTGAA GGAGCTAGCC AGGTTCAAGC CCATGCAGCG GCACCTCTTC CTTCACGAGA 
     3541 AGGCCGTCCT CTTCTGCAAG AAGAGGGAGG AGAATGGGGA GGGGTACGAG AAGGCTCCTT 
     3601 CCTACAGCTA CAAGCAGTCC TTGAATATGA CTGCTGTTGG CATCACAGAG AATGTGAAGG 
     3661 GTGACACCAA GAAGTTCGAG ATCTGGTACA ACGCAAGGGA GGAGGTTTAC ATCATTCAGG 
     3721 CACCAACTCC CGAGATTAAA GCAGCGTGGG TGAATGAGAT TCGGAAGGTG CTGACCAGCC 
     3781 AACTGCAGGC CTGCCGGGAA GCCAGCCAGC ACCGAGCCCT GGAGCAGTCC CACAGCCTTC 
     3841 CCTTGCCCAC GCCAGCCAGC ACCAGTCCCA CAAAAGGGAG CACAAGAAAT GTCAAGAAGC 
     3901 TCGAAGATCG GAAGACGGAC CCGCTCTGCC TGGAAGGCTG CGTGAGCTCA TCATTACCAA 
     3961 AGCCCCCCGA GAAGGGCAAA GACGATGCGG TCCCTAGTTC TACCTCTGAA AGCTCTGCGC 
     4021 TTTCCAGAAA GCGCTTCACG CTGCAGGGCT TGGCTAACCT CAAAGCCTCT CCGACCAGTC 
     4081 CTGACAAAAA AGCTAAGCGG CATGAAGTAA AGAGCGACCC GACTCCCTTT GGTTTGCGAG 
     4141 GCTGGAGCAA AACATCCCAC TCACTGGAGG CCCCTGAGGA GGACGGAGGC TGGTCCAGTG 
     4201 CTGAGGAGCT CATCAATTCA TCCGATGCTG AGGAAGACGG AGGAGTGGGC CCTAGGAAGC 
     4261 TGGTTCCAGG TAAATACACA GTGCTGATGG ATGGTGAGAA AGGGGGCTCT GACACCCTTG 
     4321 CCATGAGGAG TGGAGATATG GTGGAGGTTG TGGAAGAAGG AACTGAGGGC CTCTGGTATG 
     4381 TTCGGGACCT GACCAGCAGC AAGGAGGGAT GGGTGCCAGC CAGCAGCCTA GCCACCCTCC 
     4441 TGGGCAAATC CAGCTCAGCC CAGTGTCTGA GCAGCTCAGG TAAGACCCAC TGTGCGCGCC 
     4501 AGCTGTGCCC TGAGCCTGCC AAGATCCTCA GCCCAGAGCC TGTTACGCGT ACGCGGCCGC 
     4561 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4621 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4681 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4741 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4801 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4861 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4921 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4981 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     5041 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     5101 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5161 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5221 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5281 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5341 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5401 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5461 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5521 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5581 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5641 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5701 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5761 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5821 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5881 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     5941 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     6001 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     6061 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     6121 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6181 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6241 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6301 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6361 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6421 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6481 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6541 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6601 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6661 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6721 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6781 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6841 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6901 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     6961 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     7021 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     7081 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     7141 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7201 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7261 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7321 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7381 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7441 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7501 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7561 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7621 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7681 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7741 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7801 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7861 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7921 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     7981 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     8041 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     8101 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     8161 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8221 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8281 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8341 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//