LOCUS       RR208659                7362 bp    DNA     circular  SYN 
DEFINITION  Elac2 (Myc-DDK-tagged ORF) - Rat elaC homolog 2 (E. coli) (Elac2), (10 ug)
ACCESSION   NM_172326
VERSION     NM_172326.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3519..3610 
                     /label="Myc-DDK"  
     misc_feature    3640..4225 
                     /label="PolyA signal"  
     misc_feature    3697..3716 
                     /label="Restriction Enzyme"  
     misc_feature    4501..5173 
                     /label="ColE1"  
     misc_feature    5603..6397 
                     /label="Kan/Neo"  
     misc_feature    6432..6734 
                     /label="SV40 ori"  
     misc_feature    6820..7297 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3501..3507 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3501 
                     /label="Elac2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTGGGCCCTC CGCTCGCTGC TGCGTCCCCT TGGCCTGCGC ACCATGTCGC 
     1081 AGGGTTCGGC TCGCCGGCCG CGGCCTCCCA AAGACCCACT GCGACACCTG CGCACGAGGG 
     1141 AGAAGCGCGG CCCGGGTTGG GGTCCCGGGG GCCCGAACAC CGTGTACCTG CAGGTGGTGG 
     1201 CGGCGGGCGG CCGGGACGCG GCGGCTGCTC TCTACGTCTT CTCGGAATAC AACAGGTACC 
     1261 TTTTTAACTG CGGAGAAGGC GTCCAACGAC TTATGCAGGA ACACAAACTG AAAGTCGCTC 
     1321 GCTTGGACAA CATATTTCTC ACTCGGATGC ATTGGTCAAA TGTTGGGGGT TTGTGTGGAA 
     1381 TGATTTTAAC TTTAAAGGAA ACCGGGCTTC CCAAATGCGT TCTGTCTGGA CCTCCACAAC 
     1441 TGGAGAAATA TCTAGAAGCA ATCAAAATAT TTTCTGGTCC ATTGAAAGGA ATAGACCTGG 
     1501 CTGTGCGGCC TCACTCTGCA CCAGAATACA AGGATGAGAC CATGACTGTT TACCAGGTCC 
     1561 CTATCCACAG TGAACGGAGG TGTGGAGAGC AAGAGCCATC ACGGAGCCCC AAAAGATCTC 
     1621 CCAACAGGCT CAGTCCCAAA CAGTCGTCCT CAGACCCTGG ATCCGCTGAA AATGGGCAGT 
     1681 GCCTTCCAGA GGGCAGCAGT GCAGGTGTCA ACGGGAAAGC ATGGGGCAGG GACCCCTCCT 
     1741 TAGTGGTAGC TTTTGTCTGC AAGCTTCACT TGAGGAAAGG AAACTTCTTG GTGCTCAAAG 
     1801 CAAAGGAGCT GGGCCTTCCT GTTGGGACGG CCGCCATTGC ACCCATCATT GCTGCTGTCA 
     1861 AGGACGGGAA GAGTATCACT TATGAAGGAA GGGAGATTGC TGCTGAAGAG CTTTGTACAC 
     1921 CACCAGATCC TGGTCTCGTG TTCATCGTGG TAGAGTGTCC CGATGAAGGA TTCATCCAGC 
     1981 CCATCTGTGA GAATGACACC TTTCAAAGGT ACCAGGGAGA GGCCGATGCA CCTGTGGCAG 
     2041 TGGTGGTCCA CATAGCCCCA GAATCTGTAC TCATCGACAG CAGGTACCAG CAGTGGATGG 
     2101 AGAGGTTCGG GCCTGACACA CAGCATCTGA TTCTGAATGA GAACTGCCCC TCAGTCCACA 
     2161 ACCTGCGCAG CCACAAGATT CAGACTCAGC TCAGCCTCAT CCACCCGGAC ATCTTCCCTC 
     2221 AGCTCACCAG CTTCCACAGT AAGGAGGAAG GTTCTACCTT CAGCTTGCCG ACAGTTCGGG 
     2281 GTGAATGCCT TCTCAAGTAT CACGTCCGCC CCAAGAGAGA GTGGCAGAGG GACACCACAC 
     2341 TCGACTGCAA TACTGATGAA TTCATAGCTG AGGCCTTGGA GCTCCCCAAT TTCCAGGAGA 
     2401 GTGTGGAGGA GTACAGGAAG AATATGCAGG CCAGCCCAGC CCCAGCAGAG AAAAGAAGCC 
     2461 AGTATCCTGA AATTGTCTTC CTGGGTACGG GGTCTGCCAT CCCAATGAAG ATCCGAAATG 
     2521 TCAGTTCCAC ACTCGTCAAC CTAAGCCCTG ACAAGTCTGT GCTGCTGGAT TGTGGAGAAG 
     2581 GCACTTTTGG GCAGTTATGC CGTCATTATG GACAGCAAAT AGACAGAGTC CTCTGCAACC 
     2641 TTACTGCTGT GTTTGTGTCC CACCTGCATG CGGACCACCA CACGGGCTTG TTGAATATCT 
     2701 TGCTGCAGAG GGAGCATGCG CTGGCGTCTC TGGGGAAACC TTTTCAGCCC TTGCTAGTGG 
     2761 TGGCCCCTAC CCAGCTCAGG GCCTGGCTGC AGCAGTATCA CAATCAGTGC CAGGAGATTC 
     2821 TGCATCACAT CAGTATGATT CCTGCCAAAT GCCTTCAGAA AGGAGCAGAG GTCCCCAGTC 
     2881 CCCCAGTTGA AAGGCTGATC AGCTTGCTGT TGGAAACATG TGACTTACAA GAATTTCAGA 
     2941 CCTGCCTGGT ACGTCACTGC AAGCATGCTT TTGGCTGTGC GCTGGTGCAT TCATCTGGCT 
     3001 GGAAAGTGGT CTACTCAGGG GACACCATGC CCTGTGAGGC TCTGGTCCAG ATGGGGAAAG 
     3061 ATGCCACCCT CCTGATACAC GAAGCCACTC TGGAGGATGG CTTGGAAGAG GAAGCAGTGG 
     3121 AGAAGACGCA TAGTACCACC TCCCAGGCTA TTGGTGTGGG GATGCGGATG AATGCTGAGT 
     3181 TCATCATGCT GAACCACTTC AGTCAGCGGT ACGCCAAGAT CCCCCTTTTC AGCCCTGACT 
     3241 TCAATGAGAA AGTTGGCATC GCCTTTGACC ACATGAAGGT CTGCTTTGGA GACTTCCCGA 
     3301 CAGTGCCCAA GCTTATTCCT CCGCTGAAGG CCCTGTTTGC AGATGACATT GAAGAGATGG 
     3361 TGGAACGCAG GGAGAAGAGG GAGCTACGGC TGGTGCGGGC AGCCCTCCTG ACTCAGCAGG 
     3421 CAGACAGCTC AGAGGACAGA GAACCCCACC AGAAGCGGGC CCACTCAGAG GAACCACACA 
     3481 GCCCACAGAG CAAGAAGGTC AGAGCCCAGA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     3541 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     3601 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     3661 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     3721 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     3781 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     3841 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     3901 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     3961 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     4021 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     4081 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     4141 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     4201 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     4261 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     4321 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     4381 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     4441 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     4501 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     4561 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     4621 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     4681 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     4741 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     4801 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     4861 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     4921 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     4981 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     5041 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     5101 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     5161 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     5221 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     5281 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     5341 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     5401 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     5461 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     5521 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     5581 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     5641 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     5701 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     5761 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     5821 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     5881 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     5941 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     6001 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     6061 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     6121 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     6181 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     6241 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     6301 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     6361 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     6421 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     6481 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     6541 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     6601 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     6661 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     6721 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     6781 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     6841 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     6901 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     6961 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     7021 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     7081 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     7141 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     7201 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     7261 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     7321 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//