LOCUS       RR208638                8874 bp    DNA     circular  SYN 
DEFINITION  Xdh (Myc-DDK-tagged ORF) - Rat xanthine dehydrogenase (Xdh), (10 ug)
ACCESSION   NM_017154
VERSION     NM_017154.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5031..5122 
                     /label="Myc-DDK"  
     misc_feature    5152..5737 
                     /label="PolyA signal"  
     misc_feature    5209..5228 
                     /label="Restriction Enzyme"  
     misc_feature    6013..6685 
                     /label="ColE1"  
     misc_feature    7115..7909 
                     /label="Kan/Neo"  
     misc_feature    7944..8246 
                     /label="SV40 ori"  
     misc_feature    8332..8809 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5013..5019 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5013 
                     /label="Xdh"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACTGCGGAT GAGTTGGTCT TCTTTGTGAA TGGCAAAAAG GTGGTGGAGA 
     1081 AAAATGCGGA CCCTGAAACA ACACTTCTGG TCTACCTGAG AAGAAAGTTG GGGCTATGTG 
     1141 GGACCAAGCT TGGCTGTGGA GAAGGTGGCT GTGGGGCATG CACCGTGATG ATCTCCAAGT 
     1201 ATGACCGTCT TCAGAACAAG ATTGTTCATT TTTCTGTCAA TGCCTGCTTG GCTCCCATCT 
     1261 GCTCCTTGCA CCATGTTGCT GTGACCACCG TGGAAGGCAT AGGAAACACC CAGAAGCTGC 
     1321 ATCCTGTACA GGAGAGAATT GCCAGAAGCC ATGGTTCCCA GTGTGGGTTC TGCACTCCTG 
     1381 GCATTGTCAT GAGTATGTAC ACACTGCTCC GGAACCAGCC TGAGCCTACT GTTGAGGAGA 
     1441 TCGAGAATGC CTTCCAAGGA AACCTCTGTC GCTGTACAGG CTACAGACCC ATCCTCCAGG 
     1501 GATTCCGGAC CTTTGCCAAG GATGGTGGGT GCTGTGGAGG GAGTGGAAAC AACCCAAACT 
     1561 GCTGTATGAA CCAGACGAAA GACCAAACGG TTTCTCTCTC ACCTTCTTTA TTCAACCCAG 
     1621 AGGATTTCAA ACCTTTAGAT CCCACGCAAG AGCCCATCTT CCCCCCAGAG TTGCTGAGGC 
     1681 TGAAAGACAC TCCCCAGAAG AAGCTGCGTT TTGAAGGGGA ACGTGTGACC TGGATCCAGG 
     1741 CTTCAACTAT GGAGGAGCTG CTTGACCTGA AAGCTCAGCA CCCTGATGCC AAGCTGGTGG 
     1801 TGGGAAACAC AGAGATAGGC ATTGAAATGA AATTTAAGAA TATGCTATTT CCTCTGATCG 
     1861 TCTGCCCAGC CTGGATCCCT GAACTGAATT CAGTGGTGCA TGGGCCTGAG GGAATCTCCT 
     1921 TCGGAGCTTC TTGCCCCCTT AGCTTGGTGG AAAGTGTCCT GGCGGAGGAG ATTGCTAAAC 
     1981 TTCCAGAGCA AAAGACAGAG GTGTTCAGAG GCGTGATGGA GCAGCTGCGC TGGTTTGCCG 
     2041 GCAAGCAGGT CAAGTCCGTG GCGTCCATCG GAGGGAACAT CATCACTGCC AGCCCCATCT 
     2101 CTGACCTCAA CCCTGTGTTC ATGGCCAGTG GAGCCAAGCT GACTCTGGTG TCTAGAGGTA 
     2161 CCAGGAGAAC TGTTCGGATG GATCATACCT TCTTCCCTGG CTACAGAAAG ACTCTGCTCA 
     2221 GACCAGAGGA GATATTGCTG TCCATCGAGA TCCCCTATAG CAAGGAGGGA GAGTTTTTCT 
     2281 CAGCCTTCAA GCAGGCCTCC AGGAGGGAAG ATGACATTGC CAAGGTGACT AGTGGCATGA 
     2341 GAGTCCTGTT CAAACCGGGG ACCATTGAAG TGCAGGAACT GTCCCTTTGC TTCGGAGGGA 
     2401 TGGCCGACAG AACTATCTCA GCCCTCAAGA CCACTCCGAA GCAGCTATCG AAGTCCTGGA 
     2461 ATGAGGAGCT GCTGCAGAGC GTGTGTGCCG GCTTGGCAGA GGAGCTGCAG CTGGCCCCCG 
     2521 ATGCCCCTGG TGGTATGGTG GAATTCCGGC GCACCCTCAC CCTCAGCTTC TTCTTCAAGT 
     2581 TCTACCTGAC AGTGCTCCAG AAGCTGGGCA GAGCGGACCT TGAGGATATG TGTGGTAAAC 
     2641 TGGACCCCAC CTTTGCCAGT GCCACCCTGC TCTTTCAGAA GGACCCTCCA GCTAATGTCC 
     2701 AGCTTTTCCA AGAGGTGCCA AAGGATCAGT CTGAGGAGGA CATGGTGGGC CGGCCCCTGC 
     2761 CTCACCTGGC GGCAAACATG CAGGCATCGG GAGAGGCCGT GTACTGTGAT GACATTCCCC 
     2821 GCTATGAGAA TGAGCTCTCT CTCAGGCTGG TCACCAGCAC CCGGGCGCAT GCTAAAATCA 
     2881 CGTCCATCGA CACTTCAGAA GCCAAGAAGG TGCCAGGGTT TGTTTGCTTC CTCACCGCAG 
     2941 AGGATGTCCC TAATAGTAAT GCAACCGGCC TTTTCAATGA TGAAACTGTC TTTGCGAAGG 
     3001 ATGAGGTTAC TTGTGTTGGG CACATCATTG GTGCTGTGGT CGCTGACACC CCAGAACACG 
     3061 CACAGAGAGC TGCGAGAGGG GTGAAAATCA CCTATGAAGA TCTTCCAGCC ATTATCACAA 
     3121 TCCAGGATGC TATAAACAAC AACTCCTTTT ATGGCTCTGA GATAAAAATT GAGAAAGGAG 
     3181 ATCTCAAGAA AGGCTTTTCA GAAGCTGACA ATGTTGTCTC AGGAGAGTTG TATATCGGTG 
     3241 GCCAGGAGCA CTTCTACCTG GAGACCAACT GCACCATTGC CGTGCCAAAA GGCGAGGCAG 
     3301 GCGAGATGGA GCTGTTCGTG AGCACACAGA ACACCATGAA AACCCAGAGC TTTGTTGCAA 
     3361 AAATGTTGGG CGTTCCGGAC AACAGAATCG TAGTCCGAGT GAAGAGGATG GGTGGAGGCT 
     3421 TTGGAGGGAA GGAGACCCGG AGCACTGTGG TGTCCACAGC ACTGGCCTTG GCTGCACACA 
     3481 AGACTGGCCG GCCCGTACGT TGCATGTTGG ACCGAGATGA GGACATGCTG ATAACTGGTG 
     3541 GCAGACATCC CTTCCTGGCT AAATACAAGG TTGGCTTCAT GAAGACTGGG ACTGTAGTGG 
     3601 CTCTCGAGGT GGCTCACTTC AGCAATGGTG GTAACACTGA GGATCTCTCT CGGAGTATAA 
     3661 TGGAACGAGC TTTGTTCCAC ATGGATAACG CCTATAAGAT CCCCAACATT CGAGGCACTG 
     3721 GGAGGATTTG CAAGACTAAT CTGCCCTCCA ACACAGCCTT CAGAGGTTTT GGGGGTCCTC 
     3781 AGGGGATGCT AATCGCAGAA TACTGGATGA GCGAGGTCGC CATAACCTGT GGGCTGCCTG 
     3841 CAGAGGAGGT ACGGAGGAAA AACATGTACA AAGAAGGGGA CCTGACTCAC TTCAACCAGA 
     3901 AGCTGGAGGG GTTCACCTTG CCCAGGTGCT GGGATGAATG CATCGCCAGC TCTCAGTATC 
     3961 TTGCTCGCAA GAGGGAAGTG GAGAAATTCA ACAGGGAGAA TTGTTGGAAA AAGAGAGGGC 
     4021 TGTGTATAAT CCCAACTAAG TTTGGAATAA GCTTTACACT TCCTTTTCTG AACCAGGGAG 
     4081 GCGCTCTGGT TCACGTGTAC ACTGATGGTT CGGTGCTGTT GACCCATGGA GGGACTGAGA 
     4141 TGGGCCAAGG CCTTCACACC AAGATGGTTC AGGTGGCCAG CAGAGCTCTG AAAATCCCCA 
     4201 CCTCCAAGAT TCATATAAGT GAGACAAGCA CTAACACCGT CCCCAACACT TCTCCCACAG 
     4261 CTGCCTCTGC CAGTGCTGAC CTCAATGGAC AGGGTGTTTA TGAAGCATGC CAGACCATAC 
     4321 TGAAAAGGCT GGAACCTTTC AAGAAGAAGA AACCCACCGG CCCCTGGGAG GCATGGGTGA 
     4381 TGGACGCCTA TACGAGCGCA GTGAGTTTGT CCGCAACTGG ATTTTATAAG ACACCCAACC 
     4441 TTGGCTACAG CTTTGAGACA AACTCCGGAA ATCCCTTCCA CTATTTCAGT TATGGGGTGG 
     4501 CTTGCTCTGA AGTAGAAATT GACTGCTTAA CAGGGGATCA TAAGAATCTC CGTACGGATA 
     4561 TCGTCATGGA TGTTGGTTCC AGCTTGAATC CTGCCATTGA TATTGGACAA GTAGAGGGGG 
     4621 CATTTGTCCA GGGCCTTGGT CTCTTCACTA TGGAGGAGCT GCACTACTCC CCTGAGGGGA 
     4681 GCCTGCATAC TCGTGGCCCC AGTACCTACA AAATCCCTGC ATTTGGTAGC ATCCCCATTG 
     4741 AGTTCAGAGT ATCCCTACTC CGGGACTGCC CCAACAAGAG GGCCATCTAT GCATCCAAGG 
     4801 CTGTTGGGGA GCCACCTCTT TTCCTGGCTT CCTCTATCTT CTTTGCCATC AAAGATGCCA 
     4861 TTCGTGCAGC TCGAGCTCAG CACGGAGATA ACGCAAAACA ACTTTTCCAG CTAGACAGCC 
     4921 CTGCCACTCC GGAGAAGATC CGAAACGCCT GTGTGGACCA GTTCACCACC CTGTGTGTCA 
     4981 CTGGAGTACC AGAAAACTGT AAATCCTGGT CTGTGAGGAT CACGCGTACG CGGCCGCTCG 
     5041 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     5101 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     5161 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     5221 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     5281 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     5341 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     5401 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5461 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5521 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5581 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5641 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5701 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5761 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5821 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5881 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5941 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     6001 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     6061 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     6121 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     6181 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     6241 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     6301 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     6361 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6421 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6481 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6541 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6601 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6661 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6721 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6781 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6841 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6901 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6961 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     7021 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     7081 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     7141 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     7201 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     7261 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     7321 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     7381 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7441 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7501 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7561 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7621 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7681 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7741 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7801 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7861 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7921 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7981 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     8041 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     8101 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8161 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     8221 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     8281 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     8341 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     8401 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8461 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8521 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8581 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8641 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8701 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8761 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8821 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//