LOCUS       RR208609                9042 bp    DNA     circular  SYN 
DEFINITION  Map3k4 (Myc-DDK-tagged ORF) - Rat mitogen activated protein kinase kinase kinase 4 (Map3k4), (10 ug)
ACCESSION   NM_001107456
VERSION     NM_001107456.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5199..5290 
                     /label="Myc-DDK"  
     misc_feature    5320..5905 
                     /label="PolyA signal"  
     misc_feature    5377..5396 
                     /label="Restriction Enzyme"  
     misc_feature    6181..6853 
                     /label="ColE1"  
     misc_feature    7283..8077 
                     /label="Kan/Neo"  
     misc_feature    8112..8414 
                     /label="SV40 ori"  
     misc_feature    8500..8977 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5181..5187 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5181 
                     /label="Map3k4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAACACACCG AGTCAGTCTC CACATAAAGA CTTGGGGAAG GGAGTGGAGG 
     1081 CCGTGGAGGA GTACAGCTAC AAGCAGGGGA AGAAGACGCG AGCGACTCTG AGGACAACAG 
     1141 AGCGAGACCA TAAGAAAAAT GCACAGTGCT CGTTCATGTT GGACTCAGTG GCCGGGTCTT 
     1201 TGCCAAAAAA ATCTATTCCA GATGTGGATC TCAATAAGCC TTACCTCAGC CTCGGCTGTA 
     1261 GCAACGCCAA GCTGCCAGTC TCTATGCCCA TGCCTATAGC CAGAACTGCG CGGCAGACTT 
     1321 CCCGGACGGA CTGCCCAGCG GATCGGTTAA AGTTCTTTGA AACACTGCGC CTTTTGCTAA 
     1381 AGCTTACCTC AGTCTCGAAG AAGAAGGACA GGGAACAGAG GGGACAGGAA AACACGGCTG 
     1441 CTTTCTGGTT CAACCGGTCA AATGAACTGA TCTGGTTAGA GCTCCAGGCC TGGCACGCAG 
     1501 GCCGCACCAT CAATGACCAG GACCTCTTTT TATACACAGC CCGCCAAGCC ATCCCAGATA 
     1561 TTATCAATGA AATCCTCACA TTCAAAGTTA ATTATGGAAG CGCTGCCTTC TCTAGAAATG 
     1621 GAGCTGGTTT CAACGGTACC TCGCTAGAAG GTCAGTGCAG AACCCTTCAT GGGACGAACA 
     1681 GTCTAGGCTG TTCAACCTAC CACGAGCACC TCCAGCGCCA GAGGGTCTCA TTTGAGCAGG 
     1741 TGAAACGGAT AATGGAGCTG CTAGAGTACA TGGAAGCACT TTATCCGTCC TTGCAGGCTC 
     1801 TGCAGAAGGA CTACGAGAGA TACGCCGCAA AGGACTTTGA GGACAGAGTG CAGGCACTCT 
     1861 GCCTGTGGCT AAACATCACG AAAGATTTAA ATCAGAAGTT GAGGATTATG GGCACTGTTC 
     1921 TGGGCATCAA GAACTTATCA GACATTGGCT GGCCAGTGTT TGAAATCCCT TCCCCTCGGC 
     1981 CATCCAAAGG CTACGAGCCT GAGGAGGAGG GCGAGGACAC AGAGGGAGAG CTGAGGGAGC 
     2041 TGGAGAGTGG GACGGAGGAG AGTGATGAGG AGCGAAGTCC CAATCCGAGG GTGCCAGAGC 
     2101 TCAGGCTGTC TGCCGACACC ATCTTGGACA GTCACTCCCA GGGCTGCGCG TCCAGGAAAC 
     2161 TTGAGAGGCT CGAGTCCGAG GAGGACTCTG TAGGCTGGGG GATAGCAGAC TGTGGTCCCG 
     2221 AAGCCAGTAG GCATTGTTTG ACTTCTATCT ATAGACCGTT CGTGGACAAA GCACTGAAGC 
     2281 AAATGGGGCT GAGGAAGTTA ATTTTACGAC TTCATAAGCT AATGAATGGC TCCTTGCAAA 
     2341 GGGCTCGTGT AGCTCTGGTA AAGGATGACC GTCCTGTGGA GTTCTCTGAC TTTCCAGGTC 
     2401 CCATGTGGGG CTCGGATTAT GTGCAGTTGT CGGGGACGCC TCCTTCCTCG GAGCAGAAGT 
     2461 GCAGTGCTGT GTCCTGGGAA GAGCTGAGGG CCATGGACCT GCCCTCCTTT GAGCCCGCCT 
     2521 TCCTGGTGCT CTGCCGGGTC CTGCTGAACG TGATCCATGA GTGCCTGAAG CTGCGGCTGG 
     2581 AACAGAGGCC GGCCGGGGAG CCTTCCCTCT TGAGTATCAA GCAGGTGTAT TTTGACTACA 
     2641 TGAGAAGCTG GATCCAAATG CTACAGCAGT TACCTCAGGC TTCCCATAGC TTAAAAAACC 
     2701 TGCTAGAAGA AGAATGGAAT TTCACCAAAG AAATAACCCA TTACATCCGG GGAGGAGAAG 
     2761 CACAGGCTGG AAAGCTTTTC TGTGACATTG CAGGGATGCT GCTGAAATCC ACGGGGAGCT 
     2821 TTCTGGACTC TGGCCTACAG GAGAGCTGTG CTGAACTGTG GATCAGCGCC GACGACAACG 
     2881 GTGCTGCCGA CGAGCTAAGG AGGTCTGTCA TCGAGATCAG CCGAGCACTC AAGGAGCTCT 
     2941 TCCATGAGGC CCGGGAAAGA GCCTCCAAGG CCCTGGGCTT TGCTAAAATG CTGAGGAAGG 
     3001 ACCTAGAAAT AGCAGCAGAG TTTGTTCTGT CTGCGTCTGC CCGAGAGCTC CTAGACGTTC 
     3061 TGAGAGCAAA GCAGTATGTT AAGGTACAGA TTCCTGGGCT AGAGAATTTG CACGTGTTTG 
     3121 TCCCCGACAG CCTCGCTGAG GAGAAGAAAA TTATTTTGCA GCTACTCAAT GCTGCCACAG 
     3181 GAAAGGACTG CTCAAAGGAT CCGGACGACG TCTTTGTGGA TGCCTTCCTG CTCCTGACCA 
     3241 AGCATGGGGA CCGAGCCCGT GACTCAGAAG ATGGCTGGAG CACATGGGAA GCACGAGCTA 
     3301 TCAAAATTGT GCCTCAGGTG GAGACCGTGG ACACCCTGAG AAGCATGCAG GTGGACAACC 
     3361 TTCTGCTGGT TGTCATGGAG TCTGCTCACC TCGTACTTCA GAGAAAGGCC TTCCAGCAGT 
     3421 CCATTGAGGG GCTGATGACT GTACACCATG AGCAGACATC CAGCCAGCCA GTCATTGCCA 
     3481 AAGCTTTACA GCAGCTAAAG AACGATGCGC TCGAGCTGTG CAACAGAATA AGCGATGCCA 
     3541 TCGACCGCGT GGACCACATG TTCACGCTGG AGTTGGATGC CGAGGTCGAG GAGTCCGAGT 
     3601 CGGCCACGCT GCAGCAGTAC TACCGAGAGG CCATGATTCA GGGCTACAAC TTCGGGTTTG 
     3661 AGTATCATAA AGAAGTTGTT CGTTTGATGT CTGGAGAATT CAGACAGAAG ATAGGAGACA 
     3721 AATATATAAG CTTTGCCCAG AAGTGGATGA ATTATGTGCT GACCAAATGT GAGAGCGGCA 
     3781 GAGGCACAAG ACCCAGATGG GCCACCCAAG GTTTTGATTT CCTACAAGCC ATTGAACCTG 
     3841 CCTTTATTTC AGCTTTACCA GAAGATGACT TCTTGAGTTT GCAAGCCCTG ATGAATGAGT 
     3901 GCATTGGGCA CGTCATAGGA AAGCCACACA GCCCTGTCAC AGCTATCCAT CGGAACAGCC 
     3961 CCCGCCCTGT GAAGGTGCCC CGATGCCACA GTGACCCTCC TAACCCTCAC CTCATCATCC 
     4021 CAACTCCAGA GGGATTCAGG GGTTCCAGCG TCCCTGAAAA TGACCGCTTG GCCTCCATAG 
     4081 CTGCAGAACT GCAGTTCAGG TCTCTGAGTC GGCACTCGAG CCCCACGGAA GAGCGAGACG 
     4141 AGCCAGCATA TCCTCGGAGC GACTCAAGTG GATCAACACG GAGAAGCTGG GAACTTCGAA 
     4201 CACTCATCAG CCAGACCAAA GACTCGGCCT CTAAGCAGGG GCCCATAGAG GCCATCCAGA 
     4261 AGTCAGTCCG ACTGTTTGAA GAGAGGAGGT ATCGAGAGAT GAGGAGGAAG AATATCATCG 
     4321 GTCAAGTGTG CGATACCCCT AAGTCCTATG ATAACATCAT GCATGTTGGA CTGAGGAAGG 
     4381 TGACGTTTAA GTGGCAAAGA GGAAACAAAA TCGGAGAAGG GCAGTATGGA AAAGTGTACA 
     4441 CCTGCATCAG CGTCGACACA GGGGAGCTGA TGGCCATGAA GGAGATTCGA TTTCAGCCTA 
     4501 ACGACCATAA GACTATCAAG GAAACTGCAG ACGAGTTGAA AATATTTGAA GGCATCAAGC 
     4561 ACCCCAACCT GGTCCGGTAC TTTGGTGTGG AGCTTCACAG GGAAGAGATG TACATCTTCA 
     4621 TGGAGTACTG TGATGAAGGC ACACTAGAGG AGGTGTCAAG GCTGGGCCTT CAGGAGCACG 
     4681 TCATCCGGTT GTATTCCAAG CAGATCACTG TCGCCATCAA CGTCCTCCAT GAGCATGGCA 
     4741 TCGTTCACCG AGACATCAAA GGTGCCAATA TCTTCCTTAC ATCGTCTGGA CTAATCAAAC 
     4801 TGGGAGATTT TGGATGCTCA GTGAAACTTA AAAACAATGC TCAGACCATG CCTGGCGAGG 
     4861 TGAACAGCAC CCTAGGGACA GCAGCTTACA TGGCCCCTGA AGTTATTACC CGAGCTAAAG 
     4921 GAGAAGGCCA CGGACGTGCG GCAGACATCT GGAGTCTGGG GTGCGTCGTC ATAGAGATGG 
     4981 TGACTGGCAA GCGGCCTTGG CATGAGTTTG AACACAACTT TCAGATTATG TACAAGGTGG 
     5041 GAATGGGACA CAAGCCACCA ATCCCCGAAA GGTTAAGCCC TGAAGGAAAG GACTTTCTCT 
     5101 CGCACTGCCT GGAAAGTGAC CCGAAGATAA GGTGGACAGC CAGCCAGCTC CTTGACCACG 
     5161 CTTTTGTCAA GGTTTGCACA GATGAAGAGA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     5221 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     5281 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     5341 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     5401 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     5461 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     5521 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     5581 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     5641 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     5701 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     5761 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     5821 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     5881 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     5941 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     6001 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     6061 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     6121 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     6181 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     6241 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     6301 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     6361 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     6421 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     6481 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     6541 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     6601 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     6661 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     6721 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     6781 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     6841 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     6901 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     6961 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     7021 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     7081 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     7141 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     7201 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     7261 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     7321 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     7381 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     7441 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     7501 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     7561 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     7621 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     7681 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     7741 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     7801 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     7861 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     7921 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     7981 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     8041 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     8101 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     8161 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     8221 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     8281 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8341 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8401 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     8461 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     8521 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     8581 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     8641 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     8701 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     8761 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     8821 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     8881 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     8941 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     9001 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//