LOCUS       RR208604                8241 bp    DNA     circular  SYN 
DEFINITION  Tek (Myc-DDK-tagged ORF) - Rat TEK tyrosine kinase, endothelial (Tek), (10 ug)
ACCESSION   NM_001105737
VERSION     NM_001105737.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4398..4489 
                     /label="Myc-DDK"  
     misc_feature    4519..5104 
                     /label="PolyA signal"  
     misc_feature    4576..4595 
                     /label="Restriction Enzyme"  
     misc_feature    5380..6052 
                     /label="ColE1"  
     misc_feature    6482..7276 
                     /label="Kan/Neo"  
     misc_feature    7311..7613 
                     /label="SV40 ori"  
     misc_feature    7699..8176 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4380..4386 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4380 
                     /label="Tek"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACTCTTTA GCCGGCTTAG TTCTCTGTGG AGTCAGCTTG CTCCTTTATG 
     1081 GACTAGTAGA AGGAGCCATG GACCTGATCT TGATCAATTC CCTACCTCTT GTGTCTGATG 
     1141 CCGAAACATC CCTCACCTGC ATTGCTTCTG GGTGGCACCC CCACGAGCCC ATCACCATAG 
     1201 GAAGGGACTT TGAAGCCTTA ATGAACCAGC ACCAAGATCC ACTGGAAGTT ACTCAAGATG 
     1261 TGACCAGAGA ATGGGCGAAA AAGGTTGTTT GGAAGAGAGA AAAGGCCAGT AAGATTAATG 
     1321 GTGCTTATTT CTGTGAAGGT CGAGTTCGAG GACAGGCTAT AAGGATACGA ACCATGAAGA 
     1381 TGCGTCAACA AGCTTCCTTT CTACCTGCTA CTCTAACTAT GACCGTGGAC AGGGGAGATA 
     1441 ATGTGAACAT ATCCTTCAAA AAGGTGTTAA TTAAAGAAGA AGATGCAGTG ATTTACAAAA 
     1501 ATGGTTCCTT CATTCATTCA GTGCCCCGGC ATGAAGTTCC TGATATTTTA GAAGTGCATC 
     1561 TGCCTCACGC TCAGCCCCAG GATGCTGGTG TGTACTCGGC CAGGTACATA GGAGGAAACC 
     1621 TGTTCACCTC GGCCTTCACC AGGCTGATTG TCCGGAGATG TGAAGCTCAG AAGTGGGGAC 
     1681 CCGACTGTAA CCGTCCTTGT ACTACTTGCA AGAACAATGG TGTCTGCCAT GAAGATACTG 
     1741 GGGAGTGCAT TTGCCCTCCT GGGTTTATGG GGAGGACATG TGAAAAGGCT TGTGAGCCAC 
     1801 ACACATTTGG CAGGACCTGT AAGGAACGGT GTAGTGGATC AGAGGGATGC AAGTCATATG 
     1861 TGTTCTGTCT GCCAGACCCC TATGGGTGTT CCTGTGCCAC AGGCTGGAGG GGGCTGCAGT 
     1921 GCAATGAAGC ATGTCCATAC GGTCACTATG GGCCAGACTG TAAGCTCAGG TGCCACTGTA 
     1981 CCAATGAAGA GATGTGTGAC CGCTTCCAAG GATGCCTCTG CTCTCAAGGA TGGCAAGGGC 
     2041 TGCAGTGTGA GAAAGAAGGC AGGCCAAGGA TGACTCCACA GATAGAGGAT TTGCCAGATC 
     2101 ACATAGAAGT AAACAGTGGG AAATTTAACC CGATCTGCAA GGCCTCTGGC TGGCCACTAC 
     2161 CTACTAGTGA AGAAATGACC CTGGTGAAGC CGGATGGGAC AGTGCTCCAA CCAAATGACT 
     2221 TCAACCATAC AGATCATTTC TCAGTGGCCA TATTCACTGT CAACCGGATC TTACCTCCTG 
     2281 ACTCAGGAGT TTGGGTCTGC AGTGTGAACA CAGTGGCTGG GATGGTGGAA AAGCCTTTCA 
     2341 ACATTTCTGT CAAAGTTCTT CCAGAGCCCC TGCATGCCCC AAATGTTATT GACACTGGAC 
     2401 ACAACTTTGC TATCATCAAC ATCAGCTCTG AGCCTTACTT TGGGGATGGA CCGATCAAAT 
     2461 CCAAGAAGCT CTTCTATAAA CCTGTCAATC AGGCTTGGAA ATACATTCAA GTGATGAATG 
     2521 AGATTGTCAC ACTCAACTAC CTGGAGCCTC GGACTGACTA CGAGCTGTGT GTACAGCTGG 
     2581 TCCGTCCTGG AGAGGGTGGA GAAGGACATC CTGGACCTGT GAGAAGATTC ACAACAGCGT 
     2641 CTATCGGACT CCCTCCTCCA AGAGGTCTCA GTCTCCTACC CAAAAGCCAG ACAGCTCTGA 
     2701 ATTTGACTTG GCAACCGATA TTTACAAGCT CAGAAGATGA ATTTTATGTG GAAGTTGAGA 
     2761 GGTGGTCCCA GCAAACAAGA AGTGATCAGC AGAACATCAA AGTGCCTGGG AACCTGACTT 
     2821 CCGTGCTGCT GAACAACTTA CTCCCCAGGG AGCAGTACAG CGTCCGAGCT AGAGTCAACA 
     2881 CCAAGGCCCA GGGGGAGTGG AGTGAAGAAC TCAGGGCCTG GACCCTTAGT GACATTCTCC 
     2941 CTCCTCAGCC AGAAAACATC AAGATCACCA ACATCACTGA TTACACAGCT CTGGTTTCTT 
     3001 GGACAATCGT GGACGGCTAT TCGATTTCTT CCATCATCAT CCGGTATAAG GTTCAGGGCA 
     3061 AAAATGAAGA CCAGCACATT GACGTGAAGA TCAAGAATGC CACCATCACT CAATACCAGC 
     3121 TCAAGGGCCT AGAGCCAGAG ACTACATACC ATGTGGATAT TTTTGCTGAG AACAACATAG 
     3181 GATCAAGCAA CCCAGCCTTT TCCCAAGAAA TTAGGACACT TCCAGCCCCT AAAGACCTTG 
     3241 GAGGGGGAAA GATGCTACTT ATAGCCATTC TTGGGTCGGC TGGAATGACT TGCATCACCG 
     3301 TGCTATTGGC GTTTCTGATT ATGTTGCAAC TGAAGAGAGC AAATGTCCAA AGAAGAATGG 
     3361 CCCAGGCCTT CCAGAACGTG AGAGAAGAAC CAGCTGTTCA GTTCAACTCA GGAACTCTGG 
     3421 CCCTAAACAG GAAGGCCAAA AACAATCCGG ATCCCACAAT TTATCCTGTG CTTGACTGGA 
     3481 ATGACATCAA GTTCCAAGAT GTGATTGGAG AGGGCAACTT TGGCCAGGTT CTGAAGGCGC 
     3541 GCATCAAGAA GGATGGGTTA CGGATGGACG CTGCCATCAA GAGGATGAAA GAATATGCCT 
     3601 CCAAAGATGA TCACAGGGAC TTTGCAGGAG AACTGGAGGT TCTTTGTAAA CTTGGACACC 
     3661 ATCCGAACAT CATTAATCTC TTGGGAGCAT GTGAACACAG AGGCTACTTA TACCTGGCTA 
     3721 TTGAGTATGC CCCACATGGA AACCTCCTGG ACTTTCTGCG TAAGAGCCGA GTGCTAGAGA 
     3781 CAGACCCTGC CTTTGCCATC GCCAACAGCA CGGCTTCCAC ACTGTCCTCC CAGCAGCTTC 
     3841 TTCATTTTGC TGCAGATGTG GCCCGGGGGA TGGACTACTT GAGCCAAAAA CAGTTTATCC 
     3901 ACAGGGACCT GGCTGCCAGA AACATTTTAG TTGGCGAAAA CTACATAGCC AAAATAGCAG 
     3961 ATTTTGGATT GTCACGAGGT CAAGAAGTGT ATGTGAAAAA GACAATGGGA AGGCTCCCAG 
     4021 TGCGCTGGAT GGCAATTGAG TCTCTGAACT ATAGTGTCTA TACAACCAAC AGTGATGTAT 
     4081 GGTCCTATGG TGTATTGCTC TGGGAGATCG TTAGCTTAGG AGGCACTCCA TACTGCGGCA 
     4141 TGACATGTGC AGAACTCTAT GAGAAGCTGC CCCAGGGCTA CAGATTGGAG AAGCCCCTGA 
     4201 ACTGTGATGA TGAGGTGTAT GATCTAATGA GACAATGCTG GAGGGAGAAG CCTTATGAGA 
     4261 GACCATCATT TGCCCAGATA TTGGTGTCCT TAAACAGAAT GCTGGAAGAA CGAAAGACAT 
     4321 ACGTGAACAC CACACTTTAT GAGAAGTTTA CCTACGCAGG AATTGACTGT TCTGCTGAAG 
     4381 AAGCAGCCAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4441 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4501 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4561 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4621 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4681 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4741 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     4801 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     4861 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     4921 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     4981 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5041 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5101 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5161 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5221 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5281 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5341 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5401 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5461 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5521 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5581 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     5641 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     5701 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     5761 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     5821 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     5881 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     5941 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6001 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6061 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6121 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6181 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6241 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6301 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6361 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6421 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6481 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6541 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6601 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6661 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     6721 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     6781 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     6841 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     6901 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     6961 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7021 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7081 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7141 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7201 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7261 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7321 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7381 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7441 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7501 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7561 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7621 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7681 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     7741 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     7801 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     7861 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     7921 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     7981 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8041 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8101 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8161 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8221 CAAAAATTTA ACGCGAATTT T
//