LOCUS       RR208592                9321 bp    DNA     circular  SYN 
DEFINITION  Gli2 (Myc-DDK-tagged ORF) - Rat GLI-Kruppel family member GLI2 (Gli2), (10 ug)
ACCESSION   NM_001107169
VERSION     NM_001107169.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5478..5569 
                     /label="Myc-DDK"  
     misc_feature    5599..6184 
                     /label="PolyA signal"  
     misc_feature    5656..5675 
                     /label="Restriction Enzyme"  
     misc_feature    6460..7132 
                     /label="ColE1"  
     misc_feature    7562..8356 
                     /label="Kan/Neo"  
     misc_feature    8391..8693 
                     /label="SV40 ori"  
     misc_feature    8779..9256 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5460..5466 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5460 
                     /label="Gli2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGACACCAG GAGGGAAGGT ACCATTATGA CCCTCACCCT GTCCACGGTG 
     1081 TGCACGGGCC TCCCACGCTA AGTGGCAGTC CTGTCATCTC AGATATCTCC TTGATACGAC 
     1141 TTTCTCCACA CCCTGCTGGC CCTGGAGAAT CGCCCTTCAG CGCCCACCAC CCCTACATGA 
     1201 ACCCCCACAT GGAGCACTAC CTCCGGTCTG TGCACAGCAG CCCCACACTC TCAATGATCT 
     1261 CTGCCGCCAG GGGCCTCAGC CCTGCTGATG TGGCCCATGA ACATCTGAAA GAGAGGGGAC 
     1321 TATTTAGTCT CGCTGCCCCA GGCACCAACC CTTCAGACTA TTACCACCAG ATGACCCTCA 
     1381 TGGCAAGCCA CCCCACACCC TACGGGGACA TTCTAATGCA AAGTGGCGGT GCTGCTAGCG 
     1441 CACCCCATCT CCATGACTAC CTCAACCCTG TGGATGCATC ACGATTCTCC AGCCCACGTG 
     1501 TGACCCCGCG ACTGAGCCGC AAGCGGGCTC TATCCATCTC CCCGCTCTCA GATGCCAGCC 
     1561 TTGACCTACA GCGCATGATC CGGACCTCTC CCAACTCACT GGTAGCCTAC ATCAACAACT 
     1621 CCAGGAGCAG CTCAGCAGCC AGCGGTTCTT ACGGGCATCT GTCAGCTGGT GCCCTCAGCC 
     1681 CAGCCTTCAC CTTCCCCCAC CCCATCAATC CCGTGGCGTA CCAGCAGATC CTGAGCCAGC 
     1741 AGCGGGGCCT GGGTTCAGCC TTTGGACATA CACCACCCCT GATCCAGCCT TCACCCACCT 
     1801 TCTTGGCTCA GCAGCCCATG ACTCTCACCT CCATCACCGT GCCTACCCAG CTAGGCAGCA 
     1861 ATAGCAGCAA CTGTATAAGT GATGCCAACC AGAATAAGCA GAACAGCGAG TCGGCCGTGA 
     1921 GCAGCACCGT GAACCCCATC ACCATCCATA AGCGGAGCAA GGTCAAGACT GAGGCTGAGG 
     1981 GCCTACGGCC AGCCTCCCCG CTTGGACTGA CACAGGAGCA ACTGGCTGAT CTCAAGGAAG 
     2041 ATCTGGACAG GGATGACTGT AAGCAGGAGG CTGAAGTGGT CATCTACGAG ACCAACTGCC 
     2101 ACTGGGCAGA CTGCACGAAG GAATATGACA CCCAGGAGCA GCTGGTGCAT CACATCAACA 
     2161 ATGAGCACAT CCACGGGGAG AAGAAGGAGT TCGTGTGCCG CTGGCAGGCC TGCACGAGAG 
     2221 AGCAGAAGCC CTTCAAGGCC CAGTACATGC TGGTTGTCCA CATGCGCAGG CACACGGGTG 
     2281 AGAAGCCGCA CAAGTGCACG TTTGAAGGCT GCTCAAAGGC CTACTCTCGC CTGGAGAACT 
     2341 TGAAGACACA CCTGCGGTCC CACACAGGGG AGAAGCCCTA TGTGTGTGAA CACGAAGGCT 
     2401 GCAACAAAGC CTTCTCCAAT GCCTCGGACC GTGCCAAGCA CCAGAACCGC ACTCACTCCA 
     2461 ATGAGAAACC CTACATCTGT AAGATCCCAG GCTGCACTAA GAGGTACACA GACCCCAGCT 
     2521 CACTCCGCAA GCATGTGAAG ACCGTCCACG GGCCAGATGC CCATGTGACC AAGAAACAGC 
     2581 GTAATGATGT GCACCTCCGT GCTCCACTGC TCAAGGAGAA TGGGGACAAT GAGGCCGGTG 
     2641 CTGAGCCAGG TGGCCGGGGA CCCGAGGAGA GTGTGGAGGC CAGTAGCACC AGTCACACTG 
     2701 CGGAGGACTG CCTGCATATC AAAGCCATCA AGACAGAGAG CTCCGGGCTG TGCCAGTCCA 
     2761 GCCCCGGGGC CCAGTCATCC TGCAGCAGCG AGCCCTCTCC CCTGGGCAGT GTCCCCAACA 
     2821 ATGACAGTGG CGTGGAGATG CCGGGGACAG GGCCCGGGAG TCTGGGAGAC CTGACAGCAC 
     2881 TGGACGACAC GTCTCCAGGA GCTGACACAT CAGCCCTGGC TGTACCTTCC ACTGGTGGCC 
     2941 TGCAGCTGCG CAAACACATG ACCACCATGC ATCGCTTTGA GCAGCTGAAG AGAGAGAAGC 
     3001 TCAAGTCTCT AAAGGATTCC TGCTCGTGGG CTGGCCCAGC CCCACACACC CGCAACACCA 
     3061 AGCTGCCTCC CCTTCCAGTG AATGGTTCTG TTCTGGAAAA CTTCAACAAT ACTGGGGGCT 
     3121 GTGGACCAGC GGGACTGCTG CCCAACCAGC GGCTACCAGA ACTGACAGAA GTGACCATGC 
     3181 TGAGCCAGCT GCAGGAACGG AGAGACAGCT CCACCAGCAC TATGAGCTCA GCCTACACCG 
     3241 TGAGCCGCCG CTCCTCCGGC ATCTCCCCAT ACTTCTCCAG CCGCCGTTCC AGCGAGGCCT 
     3301 CGCCTCTTGG TGGCATACGC CCGCACAATG CCAGCTCAGC GGACTCCTAC GACCCCATCT 
     3361 CCACAGACGC CTCTCGGCGC TCCAGTGAGG CCAGTCAGTG CAGCGGTGGT GGCCCCGGGC 
     3421 TGCTCAACCT GACACCCGCA CAGCAGTATA GCCTGCGTGC CAAGTACGCA GCGGCCACAG 
     3481 GCGGGCCACC ACCCACGCCA CTGCCGGGCC TCGACCGCGT CAGCCTTCGT ACCCGCCTGG 
     3541 CATTACTAGA TGCTCCTGAG CGTGCACTGC CAGGCGCCTG CCCACAACCT CTGGGGCCAC 
     3601 GGCGTGGTAG CGACGGGCCT ACCTATAACC ACGGCCATGG CTATGTAGGT GCGGCTCCTG 
     3661 CCTTACCCCA TGAGGGGCCT AACGGCAGCA CACGGCGGGC CAGCGACCCT GTGCGGCGAC 
     3721 CTGACCCCCT TGTTCTGCCT CGAGTGCAAC GTTTCCACAG CATCCACAAC ATGAATCCAG 
     3781 GTCCGCTGCC CCCCTGCACT GATCGGCGTG GCCTGCATTT ACAGAGCCAC CCCAGCGTAG 
     3841 ATGGCAGCCT GACCCGCCAT GCCTACTCTC CGCGACCCCC TAGCATCAAT GAGAACGTGG 
     3901 TGATGGAAGC CGTGGCTGCT GGGGTAGAGG GTGCCGGGCT AGAGTCTGAC CTGGGGCTGG 
     3961 TGGAGGATGA CCTCGTGCTG CCAGATGATG TGGTACAGTA CATCAAGGCT CACACAGGTG 
     4021 GTGCCTTGGA TGACAGCACA CGGCAGGTGT ATCCCACAGA AGGTACTGGC TTCCCCGAGA 
     4081 ACTCTAAACT GCCCAGTCCC GGGCTACAAG GCCACCGCAG GCTAGCGGCT GCCGACTCCA 
     4141 ACATGAGCCC TTCTGCTCCT GGGCTGGGAG GCTGCCAGCT GAGCTACAGC CCCTCCTCCA 
     4201 GCCTCAACAA GAGCAACATG CCTGTGCAGT GGAACGAGGT GAGTTCTGGC ACCGTGGATG 
     4261 CCCTGCCCAC CCAGGTGAAG CCACCCTCTT TTCCTCAGAG CAACCTGGTT CAGCAGAAGC 
     4321 CAGCCTTTGC CCACTATCCA GGCTATAGCC CACAAGCCCT GCAGAGCGGC TCTGGAGGTC 
     4381 TAGACAGCAC CCAACCACAC CTACAGCTTC GAGGGGCCCC CTCTGCATCA AGAGGGAGCT 
     4441 ACACGCAACA GCCTCGACTG CCAGCTACAG GCAGTCAGTG CCTGGGTGTA ACTGCAGCCA 
     4501 TGAGCCCTCA GGCCAACTAC AGCCAAACCC ACCACCAGCT GAGTCCAAAC GTCGTCAGTG 
     4561 GATCTCTGAA CCAGTTTGCC CCCTCCTGCA GCAATATGGC AGCCAAGCCT AGCCACCTGG 
     4621 GACTCCCTCA GCAAATGGAA GTTGTCCCCA ATGCCACCAT CATAAGTGGC CATCAACGGG 
     4681 AACTTGGGAT TGCCAGTTCA TCCCTGGCTG CAGTGTCACA ACCTCACCCA GTCCTGGGCT 
     4741 ATCCCCAGCA GGACGGCTAC CAACAGGTCT CTGGCCTTCC GTCATCCCAT CAGCCTAGCT 
     4801 TCATGGAGTC CCAGCAGAAC ACGGGCTTTG GTCTCATGCA ACCTCGGCCG CCCCTGGAGC 
     4861 CCAACACAGC TAGTCGTCAC CGAGGAGTAC GTTCTGGCCA ACAGCAGCAG CAGCTGTATG 
     4921 CCAGGACCGC TGGCCAAGCC GTGGTCACAT CAGCCAACCA AGAGACAGCA GAAGCTATGC 
     4981 CCAAGGGAAC AGCAGGGACC GTGGTATCCC TAACTCCTCA GCCATCTCAG GACACAGGGC 
     5041 GGTCACAGGA CCAGAACACG CTCTACTACT ATGGCCAGAT CCACATGTAT GAACAGAACG 
     5101 GAGGCTGCCC AGCCATGCAG CCCCAGCCGC CACAACCACA AGGTTGCTCA GACAATATCC 
     5161 AGCCTGAACC TCTGCCTTCA CCAGGAGTCA ACCAGGTGTC CAGCACCGTG GACTCCCAGC 
     5221 TCCTGGAGCC CCCCCAGATT GACTTTGATG CCATCATGGA CGATGGCGAT CACTCGAGTT 
     5281 TGTTCTCTGG TGCACTGAGC CCAAGCCTGC TCCACAATCT CTCCCAGAAT TCCTCACGCC 
     5341 TCACCACACC CCGGAATTCC CTGACACTGC CCTCCATCCC CGTGGGCATC AGCAACATGG 
     5401 CTGTTGGGGA CATGAGTTCC ATGCTCACCA GCTTGGCTGA AGAGAACAAG TTTTTAAACA 
     5461 TGATGACCAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     5521 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     5581 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     5641 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     5701 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     5761 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     5821 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     5881 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     5941 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     6001 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     6061 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     6121 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     6181 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     6241 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     6301 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     6361 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     6421 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     6481 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     6541 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     6601 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     6661 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     6721 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     6781 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     6841 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     6901 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     6961 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     7021 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     7081 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     7141 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     7201 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     7261 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     7321 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     7381 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     7441 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     7501 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     7561 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     7621 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     7681 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     7741 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     7801 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     7861 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     7921 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     7981 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     8041 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     8101 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     8161 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     8221 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     8281 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     8341 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     8401 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     8461 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     8521 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     8581 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8641 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8701 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     8761 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     8821 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     8881 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     8941 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     9001 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     9061 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     9121 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     9181 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     9241 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     9301 CAAAAATTTA ACGCGAATTT T
//