LOCUS       RR208565                9090 bp    DNA     circular  SYN 
DEFINITION  Brd4 (Myc-DDK-tagged ORF) - Rat bromodomain containing 4 (Brd4), (10 ug)
ACCESSION   NM_001100903
VERSION     NM_001100903.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5247..5338 
                     /label="Myc-DDK"  
     misc_feature    5368..5953 
                     /label="PolyA signal"  
     misc_feature    5425..5444 
                     /label="Restriction Enzyme"  
     misc_feature    6229..6901 
                     /label="ColE1"  
     misc_feature    7331..8125 
                     /label="Kan/Neo"  
     misc_feature    8160..8462 
                     /label="SV40 ori"  
     misc_feature    8548..9025 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5229..5235 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5229 
                     /label="Brd4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCTACGGAG AGCGGCCCTG GGACAAGATT GAGAAATCTG CCAGTAATGG 
     1081 GGGATGGACT AGAAACCTCC CAAATGTCTA CAACGCAGGC CCAGGCCCAA CCCCAGTCAG 
     1141 CAAATGCAGC CAGCACCAAT CCTCCACCCC CAGAGACCTC CAACCCTAAC AAGCCCAAGA 
     1201 GGCAGACCAA CCAACTGCAA TATCTGCTCA GAGTGGTGCT CAAGACACTA TGGAAACACC 
     1261 AGTTTGCGTG GCCTTTCCAG CAGCCTGTGG ATGCCGTCAA GCTGAACCTC CCTGATTACT 
     1321 ATAAGATTAT TAAAACACCC ATGGATATGG GAACAATAAA GAAGCGCTTG GAAAACAACT 
     1381 ATTACTGGAA TGCTCAGGAA TGTATCCAGG ACTTCAACAC TATGTTTACA AATTGTTACA 
     1441 TCTATAACAA GCCTGGAGAT GACATCGTCT TAATGGCAGA AGCTCTGGAG AAGCTCTTCT 
     1501 TGCAGAAAAT CAATGAACTG CCTACAGAAG AAACCGAGAT CATGATAGTC CAGGCAAAGG 
     1561 GACGAGGACG AGGGAGGAAA GAAACAGGGA CAGCAAAGCC TGGTGTATCC ACGGTACCAA 
     1621 ACACAACTCA AGCATCAACT TCTCCGCAGA CCCAGATGCC TCAGCAGAAC CCTCCTCCAC 
     1681 CTGTGCAGGC CACAACTCAC CCCTTTCCTG CTGTCACCCC AGACCTCATT GCCCAGCCTC 
     1741 CTGTCATGAC AATGGTGCCC CCCCAGCCAC TTCAGACTCC TTCACCGGTA CCCCCTCAGC 
     1801 CACCACCCCC ACCTGCTCCA GTTCCACAGC CTGTGCAGAG TCACCCTCCC ATCATTGCGA 
     1861 CCACCCCACA GCCTGTGAAG ACAAAGAAAG GGGTGAAGAG GAAAGCAGAT ACCACCACCC 
     1921 CCACCACCAT CGACCCCATT CATGAGCCGC CCTCACTGGC CCCGGAGCCC AAGACCGCCA 
     1981 AGCTGGGTCC TCGGCGGGAG AGCAGCAGAC CTGTGAAGCC CCCAAAGAAG GATGTACCAG 
     2041 ACTCCCAGCA GCACCCAGGG CCAGAGAAGA GCAGCAAGAT CTCTGAGCAG CTGAAGTGCT 
     2101 GCAGTGGCAT CCTCAAGGAG ATGTTTGCTA AGAAACATGC TGCCTATGCC TGGCCTTTCT 
     2161 ACAAGCCCGT GGATGTGGAG GCACTGGGTC TGCACGACTA CTGTGACATC ATTAAACACC 
     2221 CCATGGACAT GAGCACAATC AAGTCTAAAC TAGAGTCCCG GGAGTACAGA GATGCCCAGG 
     2281 AGTTTGGTGC TGATGTCCGA TTGATGTTTT CCAACTGCTA CAAGTACAAT CCCCCTGACC 
     2341 ATGAAGTGGT AGCCATGGCT CGAAAACTCC AGGATGTGTT TGAAATGCGC TTTGCCAAGA 
     2401 TGCCTGATGA GCCTGAAGAG CCAGTTGTTA CGGTGTCCTC CCCTGCAGTG CCACCCCCTA 
     2461 CAAAGGTGGT AGCCCCACCC TCATCTAGTG ACAGCAGCAG TGATAGTTCC TCTGACAGTG 
     2521 ACAGTTCCAC TGACGACTCT GAGGAAGAGC GAGCCCAGCG GCTGGCTGAG CTCCAGGAGC 
     2581 AGCTCAAAGC CGTGCATGAG CAGCTTGCAG CCCTCTCACA GCCCCAACAG AACAAACCAA 
     2641 AGAAAAAGGA GAAGGACAAG AAGGAAAAGA AAAAGGAAAA GCACAAAAAG AAAGAAGAAA 
     2701 TGGAGGAAAA TAAAAAAAGC AAAGCCAAGG AACTTCCTCC CAAAAAGACA AAGAAAAATA 
     2761 ACAGCAGCAA CAGCAATGTG AGCAAGAAGG AACCAGCACC CACGAAGACC AAGCCGCCTC 
     2821 CCACATATGA ATCAGAAGAA GAGGACAAGT GTAAGCCTAT GTCTTATGAG GAGAAGCGAC 
     2881 AGCTAAGTCT AGATATCAAC AAACTTCCTG GTGAGAAGCT AGGCCGTGTA GTACACATAA 
     2941 TTCAGTCGAG GGAACCATCA CTTAAAAACT CCAACCCTGA TGAGATTGAG ATTGATTTTG 
     3001 AGACCCTGAA GCCATCTACA CTACGAGAGT TGGAGCGATA TGTCACCTCC TGTTTGCGGA 
     3061 AGAAAAGGAA ACCTCAAGCT GAAAAAGTTG ATGTGATTGC TGGTTCTTCC AAGATGAAGG 
     3121 GCTTCTCATC CTCTGAGTCG GAGAGCACCA GCGAATCTAG CTCCTCTGAC AGTGAAGACT 
     3181 CTGAAACAGA GATGGCTCCC AAGTCAAAAA AGAAGGGGCA CACTGGGAGG GACCAGAAAA 
     3241 AGCATCATCA CCATCACCAC CCACAGATGC AGCCAGCCCC AGCTCCTGTG CCCCAGCAGC 
     3301 CGCCGCCACC TCCACAGCAG CCTCCTCCAC CCCCACCTCC TCAGCAGCAG CAGCAGCAGC 
     3361 AGCAGCAGCA GCCCCCACCC CCACCACCTC CGCCCTCCAT GCCACAGCAG ACTGCCCCAG 
     3421 CGATGAAGTC CTCGCCCCCA CCCTTCATCA CTGCCCAGGT ACCCGTCCTG GAACCACAGC 
     3481 TACCAGGCAG TGTCTTTGAC CCTATTGGCC ACTTCACCCA GCCCATCTTG CACCTGCCGC 
     3541 AGCCAGAGCT GCCTCCTCAC CTGCCCCAGC CACCGGAGCA CAGCACTCCA CCCCATCTCA 
     3601 ACCAGCATGC TGTGGTCTCT CCTCCAGCTT TGCACAATGC GCTGCCCCAA CAACCATCTC 
     3661 GGCCCAGCAA CCGAGCTGCT GCTCTGCCCC CAAAGCCTGC CCGACCCCCA GCTGTGTCCC 
     3721 CTGCCTTGGC CCAGCCCCCC CTGCTCCCAC AGCCACCTAT GGCTCAGCCC CCCCAAGTGC 
     3781 TGCTGGAGGA TGAAGAGCCG CCTGCCCCAC CCCTCACCTC CATGCAGATG CAGTTGTACC 
     3841 TACAGCAGCT GCAGAAGGTG CAGCCTCCCA CACCACTACT CCCTTCCGTG AAGGTGCAGT 
     3901 CCCAACCCCC ACCACCTTTG CCGCCTCCAC CCCACCCTTC TGTGCAGCAA CAGCAGCTCC 
     3961 AGCAGCAGCC ACCACCACCC CCACCTCCTC AGCCACAGCC ACCACCCCAG CAACAACACC 
     4021 AGCCCCCACC ACGACCAGTT CACTTGCCAT CCATGCCCTT CTCTGCTCAC ATTCAGCAGC 
     4081 CCCCACCACC CACAGGACAG CAACCCACCC ACCCACCCCC AGGACAGCAG CCACCCCCAC 
     4141 CACAGCCTGC CAAGCCTCAG CAAGTCATCC AGCATCACCC TTCACCCCGG CACCACAAGT 
     4201 CAGACCCCTA CTCAGCTGGT CATCTTCGTG AAGCCCCTTC TCCGCTTATG ATACATTCCC 
     4261 CTCAAATGCC ACAGTTCCAG AGCCTGACCC ATCAGTCTCC ACCCCAGCAA AACGTCCAGC 
     4321 CCAAGAAGCA GGTAAAGGGC AGGGCTGAGC CACAGCCCCC AGGGCCAGTC ATGGGCCGAG 
     4381 GCCAGGGATG CCCACCTGCC TCACCGGCTG CCGTACCTAT GCTGTCCCAG GAGCTGCGCC 
     4441 CACCGTCAGT CGTCCAGCCC CAGCCCCTGG TGGTAGTGAA GGAGGAGAAG ATTCACTCAC 
     4501 CAATCATCCG CAGTGAGCCT TTTAGCACCT CACTTCGCCC AGAGCCGCCC AAGCACCCAG 
     4561 AGAACATGAA GGCCCCAGTC CACCTGCCTC AGCGGCCTGA GATGAAGCCT GTAGATGTAG 
     4621 GGAGGCCTGT GATCCGGCCT CCAGAGCAGA GTGCACCCCC ACCAGGGGCC CCTGACAAGG 
     4681 ACAAACAGAA ACAGGAGCCA AAAACACCAG TGGCGCCCAA AAAGGACCTG AAAATTAAGA 
     4741 ACATGGGCTC CTGGGCCAGC CTGGTACAGA AGCATCCGAC CACCCCATCC TCCACAGCCA 
     4801 AGTCCTCAAG CGACAGCTTT GAGCATTTCC GCCGCGCTGC TCGGGAGAAG GAGGAGCGGG 
     4861 AAAAGGCCCT GAAGGCTCAG GCTGAGCACG CAGAGAAGGA GAAGGAGCGG CTGAGGCAGG 
     4921 AGCGCATGAG GAGCCGAGAG GATGAGGATG CGCTGGAACA GGCACGTCGA GCCCATGAGG 
     4981 AGGCACGACG GCGCCAGGAG CAGCAGCAGC AGCAGCAGAG ACAGGAGCAG CAGCAGCAGC 
     5041 AGCAACAGCA GGCAGCTGCT GTGGCCGCTG CCTCCGCCCC CCAGGCCCAG AGCTCCCAGC 
     5101 CCCAGTCTAT GCTGGACCAG CAGAGGGAGT TGGCCCGAAA GCGAGAACAG GAGCGGAGGC 
     5161 GCAGGGAGGC AATGGCAGCT ACAATTGACA TGAATTTCCA GAGTGATCTT TTGTCAATAT 
     5221 TTGAAGAAAA TCTTTTTACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     5281 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     5341 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     5401 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     5461 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     5521 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     5581 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     5641 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     5701 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5761 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5821 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5881 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5941 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     6001 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     6061 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     6121 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     6181 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     6241 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     6301 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     6361 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     6421 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     6481 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     6541 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     6601 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     6661 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     6721 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6781 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6841 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6901 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6961 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     7021 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     7081 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     7141 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     7201 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     7261 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     7321 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     7381 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     7441 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     7501 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     7561 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     7621 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     7681 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     7741 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7801 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7861 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7921 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7981 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     8041 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     8101 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     8161 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     8221 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     8281 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     8341 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8401 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8461 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     8521 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     8581 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     8641 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     8701 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     8761 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     8821 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     8881 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8941 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     9001 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     9061 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//