LOCUS       RR208414                8445 bp    DNA     circular  SYN 
DEFINITION  Cilp2 (Myc-DDK-tagged ORF) - Rat cartilage intermediate layer protein 2 (Cilp2), (10 ug)
ACCESSION   NM_001107307
VERSION     NM_001107307.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4602..4693 
                     /label="Myc-DDK"  
     misc_feature    4723..5308 
                     /label="PolyA signal"  
     misc_feature    4780..4799 
                     /label="Restriction Enzyme"  
     misc_feature    5584..6256 
                     /label="ColE1"  
     misc_feature    6686..7480 
                     /label="Kan/Neo"  
     misc_feature    7515..7817 
                     /label="SV40 ori"  
     misc_feature    7903..8380 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4584..4590 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4584 
                     /label="Cilp2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCGTCACCT CTGCCCTTGC TCTATCTCTG CATCGCTGCC GCACACCTAG 
     1081 CGGGAGCGCG AGATGCCACC CCCACCGAGG AGCACACTGC AACCGTCAGG GGTCTTCAGG 
     1141 GACGGCCACC AGACCCCGGC CAGCCCTCGC CGGCCCTGGA GGACTGGGAG GAGGCCAGCG 
     1201 AGTGGACCTC GTGGTTCAAC GTGGACCACC CGGGAGGAGA TGGTGACTTC GAGAGCCTGG 
     1261 CAGCCATTCG CTTCTACTAT GGTCCCGCGC GCGTGTGCCC GAGGCCGCTG GCACTGGAGG 
     1321 CGCGCACCAC GGACTGGGCA CTACCCGCTG CAATAGGCGA GAGGGTGCAC GCCAACCCAG 
     1381 AGCGCGGATT CTGGTGTCTT AATCGCGAGC AGCCGCGAGG CCGCCGCTGC TCCAACTACC 
     1441 ACGTGCGCTT CCGCTGCCCA CTGGATCCAC CTTATCTCCC TCCTTGCTCT GCAGAGGCGG 
     1501 CATGGGGCGC TTGGGGCGCT TGGGGCCTCT GTTCCAAGAG TTGCGGGCCA GGCCGTCGCC 
     1561 TGCGCCGTCG CAGCTGTCAA AGCTCATCTG GGGACACATG TCCTGGGAGC CCCCAGGAGG 
     1621 CACAGAAGTG CGTGAGATCC CGTTGCCCAG CTCCCCCCAA GAGTTCTCTC TGCCCCTGCT 
     1681 GCTCCCATGT TCCCCTAGGA TGTAGCTCTG ACACCTGCGG GTGCCCCAAC CACATCCTCT 
     1741 TGGGCTCGGT GGTCACCCCA TCTGGGCGAC CGCTGTCAGG AGCCAGGGTC TCCCTGCGAA 
     1801 CACGACCTGG CACCGTAGCC ACCAGTGACA CCCATGGAAC CTTCCGGGTG CCTGGAGTCT 
     1861 GCGCTGGCAG CAAGGCCAAC GTCAGTGCCC GGATGAATGG CTTCTCTACT GGTACAGCCC 
     1921 AGGCCCATGC CAACACCTCC AACTCTGCTG TGGTCACCAT CGTCCTTGAA GAGTTAGGGA 
     1981 AGCCGTACCT GGTAAAGCAC CCTGAGTCTC GAGTTCGAGA GGCAGGTCAG AATGTGACCT 
     2041 TCTGCTGCAA GGCCTCTGGG ACACCCATGC CCAAGAAATA TTCCTGGTTC CACAACGGGA 
     2101 CCTTACTGGA CCGACGCCAG CAGGGGTCTG GGCCACACCT AGAGCTCCAG GGGCTTCGTG 
     2161 AGGAGCAGGC AGGGGAGTAC CACTGCAAAG CCTGGAACGA GGCGGGCACC GTGCGATCCC 
     2221 GCGCTGCCCA CCTCACGGTG CTCGCTCCCG GCCAGCCAGC CTGTGACCCC CGACCTCAGG 
     2281 AACACCTGAT CAAGCTTCCG GATGACTGCG GCCAGCCGGG TGGCGGCCCC ACGTACCTAG 
     2341 ACGTGGGACT GTGCGCAGAC ACGCGCTGCC CGGGCCCCGT GGGCTCCGGC CCGCGCTGTG 
     2401 GGGACGCGGG CTCTCGCTGC TGCTCAGTGC TGCGCCTGGA GAGCAGAGAT ATCCGCTGCT 
     2461 CTGGCTATGT GCTCCCAGTG AAGGTGGTGG CTGAGTGTGG CTGTCGCAAG TGCCTGCCCC 
     2521 GTAGGGGATT GGTGCGAGGC CGCGTGGTGG CTGCGGACTC TGGGGAGCCC CTGCGTTTCG 
     2581 CCAGGATTCT ATTGGGCCTT GCACCCATCG GCTTCACCTC CTACCAGGGC GACTTCACCA 
     2641 TCGAGGTGCC GCCGGCCACC GAGCGCCTCG TGGTGACCTT CGTGGACTCG AGTGGTGAGT 
     2701 TCGTGGACAG CATCCGCGTG TTGCCTTTCG ACCCTCGCGG CGCTGGTGTG TACCACGAGA 
     2761 TCCGCGTGCT GCGCAAGGCG GCCGCAGTGC TGCTGGACGC GGAGCAGGGC GGTGAAATTC 
     2821 CGCTGGGCGG CACAGAGGAG GCGCCAGCGC TAGGCGAGCT AGTCTTCCCG CCCCGGACCT 
     2881 TCCACCACCC CGACGGCCGT CCCTACTCGG GGCCCGTGGA GGCGCGTGTC ACCTTCGTGG 
     2941 ACCCTCGTGA CCTGGCGTCG GCGGCCGCGG CGTCCAGCGA CCTGCGCTAC CTGGATAGCT 
     3001 CGGGTGAGCT GGCGCCGCTG CGCACCTATG GCATGTTTGC AGTGGACCTG CACGCGCCAG 
     3061 GCTCCACCGA GCAGCTGCAC GTGGCGCGCG CGGACGTGCG CGTGGACGCC GACCACGTGC 
     3121 GCATGCCCGG ACACGCCGAT GCTCTGGCAC TCTGGTCGTT GGACCCAGAG ACCGGCCTGT 
     3181 GGGAGGAGGA GGGGGCTGAG CAGGGAGGAG GCGGCTTCCG GCGTGAGAAG GAGGCGGTGC 
     3241 GCGTGCGCAG GGAGGAGCGC ACGTTCCTCG TGGGCGCGCT CACGGTGCGC GAGCGCCGCT 
     3301 TATTCAACCT AGACGTGCCC GAGCGGCGCC GCTGCTTCGT GAAGGTTCGC GCCTACGGTA 
     3361 CAGATCGCTT CGCGCCCTCA GAGCAGGTGC AGGGCGTGGT GGTGACGCTG CTCAATCTGG 
     3421 AACCCGCGCC CGGTTTCACG GCCAACCCAC GCGCGTGGGG CCGCTTCGAC AGCGCGGTCA 
     3481 CAGGGCCCAA CGGCGCGTGC GTGCCGGCCT TCTGCGACGC CGAGCGGCCC GACGCCTACA 
     3541 CGGCCTTCGT GACCGCGTCT CTAGGAGGCG AGGAGCTGGA GGCCGCGCCG TCGCGGCCAC 
     3601 GCGCGACCGC GGCCACCGTG GGCGTGGCAG AGCCCTACCT GGAGCGCCTC GGCTACCAGC 
     3661 GCACCGATCA CGACGACCCC GCGCTCAAGC GCACCGGCTT CCGCTTGAAC CTTGCACGTC 
     3721 CACGCACGGG CCACGAGTCC GAGGCGCACG GGCCCGTGTA TCCGTGGCGC CGCCTGCGCG 
     3781 ACTGCGAGGA CGCGCCGGTC ACCGACAGTC ACTTCCGATT CTCGCGCGTA GAGGCAGACA 
     3841 AGTACGAGTA CGACGTGGTG CCATTCCACG AGGGAGCGCC CGCCTCGTGG ACAGGCGACC 
     3901 TGCTGGCCTG GTGGCCCAAC CCGCAGGAGT TCCGAGCGTG CTTCCTGAAG GTGCGGCTGC 
     3961 AGGGCCCCCA GGAGTACATG GTGCGTTCGC ACAACGCGGG CGGCACCCAC GAAGACACGC 
     4021 GCGGCCACCT CTATGGGCTG CGCGACACTC GCAGCGTGCG CAACCCTGAG CGCCCGGGCG 
     4081 CCTCGGCCGC CTGCGTGGAG TTTAAGTGCG GTGGCATGCT GTTTGACCAG CGCCAAGTGG 
     4141 ACAGGACACT GGTGACTGTG ACCCCGCAGG GCAGCTGCCG CCGCGTGGCC GTCAACACGC 
     4201 TCCTGCAGGA CTATCTAGCC CGGCACCCGC CGCTCGCCCC CGCGGACGAC CCCGCCACCT 
     4261 TCGCCATGCT GGCTCCGCTT GACGCCCTGG GCCACAACTA TGGTGTCTAC ACCGTCACCG 
     4321 ACCAGAGCCC ACGGCTGGCC AAGGAAATCG CCATCGGCCG CTGCTTCGAC GGCTCCTCGG 
     4381 ACGGCTTCTC CCGGGAGATG AAGGCGGACG CGGGCACAGC GGTTACCTTC CAGTGCCGAG 
     4441 AGCCGCCCGC CAGACCCAGC CTCTTCCAGA GGCTGCTGGA GAACCCCGCA TCGGCGCTTG 
     4501 GCGACATTCG TAGGGAGATG GGCCAAGCCG CCCGCGATTC CCGGGTTACC CAAACCCAGG 
     4561 CTGGGGACAC CGGCCCCTTC GGTTCTGGAC AGACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4621 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4681 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4741 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4801 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4861 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4921 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4981 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     5041 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5101 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5161 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5221 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5281 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5341 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5401 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5461 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5521 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5581 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5641 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5701 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     5761 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     5821 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     5881 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     5941 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     6001 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     6061 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6121 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6181 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6241 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6301 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6361 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6421 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6481 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6541 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6601 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6661 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6721 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6781 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     6841 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     6901 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     6961 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     7021 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     7081 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7141 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7201 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7261 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7321 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7381 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7441 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7501 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7561 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7621 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7681 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7741 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7801 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     7861 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7921 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     7981 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     8041 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     8101 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8161 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8221 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8281 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8341 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8401 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//