LOCUS       RR208372                6947 bp    DNA     circular  SYN 
DEFINITION  Prkg1 (Myc-DDK-tagged ORF) - Rat protein kinase, cGMP-dependent, type 1 (Prkg1), (10 ug)
ACCESSION   NM_001105731
VERSION     NM_001105731.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3104..3195 
                     /label="Myc-DDK"  
     misc_feature    3225..3810 
                     /label="PolyA signal"  
     misc_feature    3282..3301 
                     /label="Restriction Enzyme"  
     misc_feature    4086..4758 
                     /label="ColE1"  
     misc_feature    5188..5982 
                     /label="Kan/Neo"  
     misc_feature    6017..6319 
                     /label="SV40 ori"  
     misc_feature    6405..6882 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3086..3092 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3086 
                     /label="Prkg1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGCGAGCTG GAGGAAGACT TTGCCAAGAT TCTCATGCTC AAGGAGGAGA 
     1081 GGATCAAAGA GCTGGAGAAG CGGCTGTCAG AGAAAGAGGA AGAAATCCAG GAGCTGAAGA 
     1141 GGAAACTCCA CAAATGCCAG TCGGTGCTGC CCGTGCCCTC CACCCACATT GGCCCGCGGA 
     1201 CCACCCGGGC GCAGGGCATC TCCGCGGAGC CGCAGACCTA CAGGTCCTTC CACGACCTGC 
     1261 GCCAGGCGTT CCGGAAGTTC ACTAAATCCG AGAGGTCGAA GGATCTCATA AAGGAGGCTA 
     1321 TCCTTGACAA TGACTTCATG AAGAACTTGG AGCTGTCACA GATCCAGGAG ATTGTGGATT 
     1381 GTATGTACCC CGTGGAATAT GGGAAGGACA GCTGCATCAT CAAGGAAGGC GATGTGGGGT 
     1441 CACTGGTGTA TGTCATGGAA GATGGGAAGG TCGAAGTCAC AAAAGAAGGT GTGAAGTTGT 
     1501 GCACCATGGG TCCCGGGAAA GTGTTCGGGG AGCTGGCTAT TCTTTACAAC TGTACCCGGA 
     1561 CAGCGACCGT CAAGACTCTT GTAAATGTGA AACTCTGGGC CATTGATCGA CAATGTTTTC 
     1621 AAACAATAAT GATGAGAACA GGACTCATCA AGCACACCGA GTACATGGAG TTTTTAAAAA 
     1681 GTGTTCCAAC ATTCCAGAGC CTTCCTGATG AAATCCTCAG CAAGCTGGCT GATGTCCTCG 
     1741 AAGAGACTCA CTATGAAAAT GGAGAATATA TCATAAGGCA GGGTGCAAGA GGAGACACCT 
     1801 TCTTCATCAT CAGCAAAGGG AAGGTAAGCG TTACCCGAGA AGACTCACCA AGTGAAGACC 
     1861 CAGTCTTTCT TAGAACTTTA GGGAAGGGAG ATTGGTTTGA AGAGAAAGCC TTGCAGGGGG 
     1921 AGGATGTAAG AACAGCGAAC GTCATTGCTG CAGAGGCTGT CACCTGCCTC GTGATTGACA 
     1981 GAGACTCTTT CAAGCATTTA ATTGGAGGAC TGGATGATGT CTCTAACAAA GCATATGAGG 
     2041 ATGCAGAAGC GAAAGCAAAA TATGAAGCCG AAGCTGCCTT CTTCGCCAAC CTGAAGCTGT 
     2101 CTGACTTCAA CATCATTGAC ACCCTTGGAG TGGGAGGTTT CGGACGAGTG GAGCTGGTCC 
     2161 AGTTGAAGAG TGAAGAATCT AAAACATTTG CGATGAAGAT CCTCAAGAAA CGACACATTG 
     2221 TGGACACGAG ACAGCAGGAG CACATCCGCT CAGAGAAGCA GATCATGCAG GGGGCTCATT 
     2281 CCGATTTCAT CGTGAGGCTA TACCGGACAT TTAAAGACAG CAAATACTTG TATATGTTGA 
     2341 TGGAAGCTTG CCTAGGTGGA GAACTCTGGA CTATTCTCAG GGATAGGGGT TCATTTGAAG 
     2401 ACTCGACAAC CAGATTTTAC ACAGCATGTG TGGTAGAAGC GTTTGCCTAT CTGCATTCCA 
     2461 AAGGAATCAT TTACAGGGAC CTCAAGCCAG AAAATCTCAT CCTAGATCAC CGAGGTTATG 
     2521 CCGAACTGGT TGACTTTGGC TTTGCAAAGA AAATAGGATT TGGAAAGAAA ACATGGACTT 
     2581 TTTGTGGGAC CCCAGAATAC GTGGCCCCAG AGATCATCCT GAACAAAGGC CATGACATTT 
     2641 CTGCTGACTA CTGGTCACTA GGGATTCTGA TGTATGAGCT TCTGACTGGC AGCCCACCTT 
     2701 TCTCAGGCCC AGATCCTATG AAAACCTACA ACATCATACT GCGGGGGATT GACATGATAG 
     2761 AATTTCCAAA GAAGATTGCA AAAAGTGCTG CTAATTTAAT TAAAAAACTA TGCAGGGACA 
     2821 ACCCATCAGA AAGGTTAGGA AATTTGAAAA ACGGAGTCAA AGACATTCAA AAACACAAGT 
     2881 GGTTTGAGGG CTTTAACTGG GAAGGCTTAA GAAAAGGCAC CTTGACTCCT CCCATAATTC 
     2941 CGAGCGTTGC GTCGCCCACG GACACAAGCA ATTTTGACAG TTTCCCTGAG GACAGCGACG 
     3001 AGCCACCGCC TGATGACAAC TCAGGATGGG ACATAGACTT CAGGACAGCG ACGAGCCACC 
     3061 GCCTGATGAC AACTCAGGAT GGGACATAGA CTTCACGCGT ACGCGGCCGC TCGAGCAGAA 
     3121 ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA 
     3181 TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC 
     3241 CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA 
     3301 GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA 
     3361 TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG 
     3421 CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA 
     3481 TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC 
     3541 AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA 
     3601 TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA 
     3661 AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT 
     3721 AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG 
     3781 TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA 
     3841 TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA 
     3901 ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG 
     3961 CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC 
     4021 CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA 
     4081 TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG 
     4141 CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC 
     4201 TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC 
     4261 GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC 
     4321 CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG 
     4381 AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA 
     4441 AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT 
     4501 AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG 
     4561 CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT 
     4621 GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG 
     4681 ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT 
     4741 GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT 
     4801 TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA 
     4861 TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA 
     4921 ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT 
     4981 CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC 
     5041 TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG 
     5101 AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC 
     5161 GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC 
     5221 GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT 
     5281 CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT 
     5341 AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA 
     5401 CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA 
     5461 TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA 
     5521 GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT 
     5581 TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT 
     5641 CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG 
     5701 GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG 
     5761 CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT 
     5821 TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC 
     5881 GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC 
     5941 TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG 
     6001 ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT 
     6061 CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA 
     6121 ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC 
     6181 GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG 
     6241 CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT 
     6301 TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC 
     6361 CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC 
     6421 AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG 
     6481 GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA 
     6541 GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC 
     6601 CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG 
     6661 CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA 
     6721 AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC 
     6781 GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA 
     6841 CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT 
     6901 ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//