LOCUS       RR208361                8556 bp    DNA     circular  SYN 
DEFINITION  Wnk4 (Myc-DDK-tagged ORF) - Rat WNK lysine deficient protein kinase 4 (Wnk4), (10 ug)
ACCESSION   NM_175579
VERSION     NM_175579.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4711..4802 
                     /label="Myc-DDK"  
     misc_feature    4832..5417 
                     /label="PolyA signal"  
     misc_feature    4889..4908 
                     /label="Restriction Enzyme"  
     misc_feature    5693..6365 
                     /label="ColE1"  
     misc_feature    6795..7589 
                     /label="Kan/Neo"  
     misc_feature    7624..7926 
                     /label="SV40 ori"  
     misc_feature    8012..8489 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4686..4693 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4686 
                     /label="Wnk4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTAGCACCT CGAAATACAG AGACCGGGGT CCACATGTCC CAAACGGAGG 
     1081 CCGACCTGGC TTTGCGGCCA TCTCCGTCTC TCACCTCCAT GGGGCCGACC CGCCTGGGGC 
     1141 CACCTCCTCG CCGAGTGCGC CGCTTCTCTG GGAAGGCTGA GCCCCGGCCG CGCTCTTCGA 
     1201 GACCGAGCCG CCGCAGCTCA GTCGATCTGG GACTGTTGAG CTCCTGGTCT CAACCAGCCT 
     1261 CACTCCTTCC GGAACCTCCT GATCCTCCGG ACTCCGCTGG TCCCATGAGG AGCCCACCTT 
     1321 CAAACTCTAA AGAACACCCC GAGGGCACAT GGACGGGGGC AGCCCCGGTG AAGGCTGCAG 
     1381 ACTCTGCCTG TCCTGAGCTT ACAGTATCTT CCGGAGGTCC GGGGTCCCGG GAGCCACCAA 
     1441 GGGTTCCTGA TGCTGCAGCC CGGGAACGGC GGCGGGAGCA GGAAGAAAAA GAGGACACAG 
     1501 AGACCCAGGC TGTGGCAACG TCTCCCGACG GCCGATACCT CAAGTTTGAC ATTGAGATTG 
     1561 GAAGAGGTTC CTTCAAGACC GTGTATCGAG GGCTAGACAC CGACACCACG GTAGAGGTGG 
     1621 CCTGGTGTGA GCTGCAGGCT CGGAAACTCT CTCGGGCTGA ACGGCAGCGT TTCTCTGAGG 
     1681 AAGTTGAGAT GCTCAAGGGG CTGCAGCACC CCAACATCGT CCGCTTCTAT GACTCCTGGA 
     1741 AGTCGGTGCT GAGGGGTCAG GTTTGCATCG TGCTGGTCAC AGAACTCATG ACCTCGGGCA 
     1801 CGCTCAAGAC GTACCTGAGG AGATTCCGAG AAATGAAGCC CCGAGTCCTT CAGCGCTGGA 
     1861 GTCGCCAGAT CCTACGGGGA CTTCATTTCT TACATTCCCG AGTGCCCCCC ATCCTGCACC 
     1921 GAGATCTCAA ATGTGACAAT GTCTTCATTA CCGGCCCTTC AGGCTCTGTC AAAATCGGAG 
     1981 ATCTCGGACT GGCCACGCTC AAGCGCGCCT CCTTTGCCAA GAGCGTCATT GGGACCCCGG 
     2041 AATTCATGGC CCCTGAGATG TATGAGGAAA AGTACGACGA GGCTGTGGAT GTGTACGCCT 
     2101 TTGGCATGTG CATGCTGGAG ATGGCTACGT CTGAGTATCC CTACTCTGAG TGTCAGAACG 
     2161 CAGCACAAAT CTACCGAAAG GTCACTTCGG GCACAAAGCC CAACAGCTTC TACAAAGTGA 
     2221 AGATGCCAGA GGTGAAGGAG ATCATTGAAG GCTGCATCCG CACGGATAAG AATGAGAGGT 
     2281 TCACTATCCA GGATCTTCTG ACCCACGCGT TCTTCCGCGA GGAACGTGGT GTGCATGTGG 
     2341 AGCTGGCAGA GGAAGATGAT GGAGAGAAGC CGGGTCTCAA ACTCTGGCTG CGCATGGAGG 
     2401 ACGCACGACG CGGGGGGCGC CCAAGGGACA ACCAGGCCAT CGAGTTCCTG TTCCAGCTCG 
     2461 GTCGGGATGC AGCGGAAGAG GTGGCTCAGG AGATGGTAGC CTTGGGCTTA GTCTGCGAAG 
     2521 CAGACTACCA GCCAGTGGCC CGTGCAGTTC GAGAACGGGT TGCTGCCATC CAGCGGAAGC 
     2581 GGGAGAAGCT AAGAAAAGCC AGGGAATTGG AGGTTCTCCC ACCAGACTCA GGACCTCCTC 
     2641 CAGCAACTGT GTCTATGACT CCTGGTCCCC CCAGTGCCTT CCCCCCAGAG CCCGAGGAGC 
     2701 CAGAGGCTGA CCAGCACCAA TCCTTCCTCT TCCGCCATGC CAGCTACTCA TCCACTACAT 
     2761 CTGATTGCGA GACTGATGGC TACCTCAGCT CCTCCGGCTT CCTGGATGCC TCAGACCCTG 
     2821 CCCTTCAGCC CCCCGGGGGG ATGCCATCCA GCCCCGCTGA GCCCCATCTC TGCTTGCCCT 
     2881 CGGGTTTTGC CTTGTCCATT CCACGGTCTG GCCCTGGCAG TGACTTTTCT CCTGGGGACA 
     2941 GCTACGCCTC AGATGCAGCC TCAGGCCTCA GTGACATGGG AGAAGGGGGG CAAATGAGGA 
     3001 AAAATCCAGT GAAGACTCTG CGACGGAGAC CTCGATCCCG GCTTCGGGTC ACGAGTGTCT 
     3061 CAGACCAGAG CGACAGAGTC GTGGAGTGCC AGCTGCAAAC TCACAACAGC AAGATGGTGA 
     3121 CGTTCCGATT TGATCTGGAT GGGGACAGCC CAGAAGAAAT CGCAGCTGCC ATGGTTTATA 
     3181 ATGAATTCAT TCTGCCCTCG GAGCGAGACG GATTCCTGAG CCGGATCCGG GAGATTATCC 
     3241 AGCGAGTGGA GACCCTGCTG AAGAGAGATG CTGGTCCCTC GGAGGCCACT GAAGATGCGC 
     3301 TGAGCCCTCA GGAAGAGGCA GCAGCCATGC CTGCCCTCCC AGGCCCATCC GATGAAGAGC 
     3361 TCCAGAGAAG CATCTCCCCG GAACAGAGGA GCTGGGCAGC CTTCTCTACA TCTCCATCTT 
     3421 CTCCTGGCAC CCCCTTGTCC CCTGGAACCC CCTTTTCCCC TGGGACCCCT CCTGTCTTCC 
     3481 CATGCCCCAT CTTCCCTATC ACTTCACCCT CATGCCATCC CTACCCGTTC TCACAGGTCT 
     3541 CTTCAAATCC CTGTCCACAG GCCCCCAGCT CCCTACTTCC CTCCTCCTCC GGTGCTTCTC 
     3601 AGGTTCCATT CCCATCCCCT TCCCTTCCCA CCAGCTCTCC CCTCCCATTC TCTCCTAGTT 
     3661 ATCCCCAAGT CCCTCTTCAC CCTGCTTCTC TTCCCACCTG CCCCTCCCCT CCCCCTCTCC 
     3721 CCTCCACCAC AGCAGCCCCT CTCCTCTCTC TGGCTAGTGC CTTCTCTCTG GCTGTGATGA 
     3781 CTGTGGCCCA GTCCCTGCTG TCCCCATCCC CTGGGCTTCT CTCTCAGTCT CCTCCAGCCC 
     3841 CTCCAGGTCC TCTGCCTAGC ATGCCCCTTC CCCTTGCTTC TTGTGACCAG GAGAGCCTTT 
     3901 CAGCCCAAAC AGCTGAGACA GAGAATGAGG CTTCCCGAAA TCCTGCTCAG CCACTACTGG 
     3961 GTGATGCTAG ACTGGCACCC ATATCTGAAG AGGGAAAACC CCAGCTGGTT GGCCGATTCC 
     4021 AAGTGACTTC ATCCAAGGAA CCAGCAGAGC CTCCCCTACA ACCAGCATCT CCAACTCTCT 
     4081 CCAGATCCCT AAAGCTTCCA ACCCCTCAGC TGACCTCAGA GAGTTCAGAC ACAGAGGACA 
     4141 GTGCTGCAGG AGGACCAGAG ACCAGGGAGG CTCTGGCAGA GAGTGACCGT GCAGCCGAGG 
     4201 GCTTGGGGGT CGCCATCGAT GAGGAAAAGG ATGAAGGGAA GGAACCCCAA ATTGGGGGCA 
     4261 GTTCCCCAAT CTTGAGCCAG CCCAGCCCAG TGTGGATGAA CTACTCCTAC AGCAGCCTGT 
     4321 GTCTAAGCAG TGAGGAGTCA GAGAGCAGCG GGGAGGACGA GGAGTTCTGG GCTGAGCTGC 
     4381 AGAACCTTCG GCAGAAGCAC TTGTCGGAAG TGGAAGCACT ACAGACGCTA CAGAAGAAGG 
     4441 AAATCGAAGA CTTGTACAGC CGGCTTGGAA AGCAACCCCC ACCTGGTATT GTAGCTCCAG 
     4501 CGGCTATGCT GTCCTGCCGC CAGCGCCGCC TCTCCAAGGG AAGCTTTCCC ACCTCCCGCC 
     4561 GCAACAGCCT GCAGCGCTCT GATCTCCCTG GCCCTGGCAT CATGCGGAAG AACTCTCTGA 
     4621 GTGGCAGCAG CACCGGCTCC CAGGAGCAGC GGGCAAGCAA GGGGGTGACA TTCGCCGGGG 
     4681 ATGTTGGCAG GATGAGCGGA CCGACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4741 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4801 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4861 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4921 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4981 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     5041 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     5101 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     5161 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5221 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5281 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5341 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5401 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5461 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5521 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5581 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5641 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5701 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5761 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5821 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5881 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5941 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     6001 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     6061 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     6121 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     6181 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6241 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6301 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6361 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6421 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6481 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6541 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6601 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6661 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6721 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6781 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6841 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6901 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6961 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     7021 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     7081 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     7141 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     7201 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7261 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7321 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7381 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7441 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7501 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7561 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7621 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7681 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7741 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7801 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7861 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7921 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7981 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     8041 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     8101 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     8161 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     8221 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     8281 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8341 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8401 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8461 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8521 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//